SSGCID: Target Page

BupsA.17623.a

Outer membrane protein, OmpW family

CENTER ID:	BupsA.17623.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17623.a.A2.GE31472	Mature protein( BupsA.17623.a )		21	276

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.23
RefSeq:	YP_331444.1
UniProt:	Q3JYA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRKALCTALG VAALTPLAAH AQSAGSNVVT LGWFHVMPQQ SSTPLTTNVA PTPINTPLRL PPSFTSAGTG LSTSGADTVG LTVSHFLTDH IAVTSVAGVP PVFKVSGHGT ITPPGPAGAL GTQNIGLAQV NPIVKSVRQW SPAVVFQYYF AQATAKFRPF VGIGVSYNWF SDLQLNTNFI KQTQDNLGAI LAAGAGKPGV TQVSAKASSS WQPVFNAGLS YNITDHWGLV ASVTYIPLKT TSTVTIKAAD GTTLAESKSD LKADPIISYV AVSYKF

NT Sequence

ATGAGAAAAG CACTCTGCAC GGCGCTCGGC GTCGCGGCGC TGACGCCGCT CGCCGCGCAC GCGCAAAGCG CGGGCAGCAA CGTCGTCACG CTCGGCTGGT TTCACGTGAT GCCGCAGCAA AGCAGCACGC CGCTTACGAC GAATGTCGCG CCGACGCCGA TCAACACGCC GCTGCGTTTG CCGCCGTCGT TCACGTCGGC GGGCACCGGG CTTTCGACGA GCGGCGCGGA CACCGTCGGC CTGACCGTCA GCCACTTTCT GACCGACCAC ATCGCGGTTA CCTCGGTGGC GGGCGTGCCG CCCGTCTTCA AGGTGTCCGG CCACGGCACG ATCACGCCGC CCGGCCCGGC CGGCGCGCTC GGCACGCAGA ACATCGGGCT CGCCCAGGTC AATCCGATCG TGAAGAGCGT GCGGCAGTGG AGCCCCGCCG TCGTATTCCA GTACTACTTC GCGCAAGCCA CCGCGAAGTT CCGGCCGTTC GTCGGCATCG GCGTGTCGTA CAACTGGTTC AGCGATCTGC AACTGAACAC GAACTTCATC AAGCAGACGC AGGACAACCT CGGCGCGATT CTCGCCGCGG GCGCGGGCAA GCCGGGGGTC ACGCAGGTAT CGGCGAAGGC GTCGTCGTCG TGGCAGCCGG TGTTCAATGC GGGCCTGTCG TACAACATCA CCGATCATTG GGGGCTCGTC GCATCGGTCA CGTACATCCC GCTGAAGACG ACGTCGACGG TGACGATCAA GGCGGCCGAC GGCACGACGC TCGCCGAGTC GAAATCGGAT CTGAAGGCCG ATCCGATCAT CAGCTACGTG GCGGTGTCGT ACAAGTTC

Details for BupsA.17623.a.A2.GE31472

HARVESTED ON:	2/1/2011
SEQUENCED ON:	2/8/2011
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	67
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAQSAGSNV VTLGWFHVMP QQSSTPLTTN VAPTPINTPL RLPPSFTSAG TGLSTSGADT VGLTVSHFLT DHIAVTSVAG VPPVFKVSGH GTITPPGPAG ALGTQNIGLA QVNPIVKSVR QWSPAVVFQY YFAQATAKFR PFVGIGVSYN WXSDLQLNTN FIKQTXXTSA RFSXGXXAGX TQXSXXXRXX XSSXXXXXXX XXXXXXXXXR XXXXXXXXXX XXXXXXXXXX XXXXSXXXXX XXKXXXXXXX XXXXXXXXXX X

Validated NT Sequence

gnnnnnnnnn nnnnnnnnnn gannnnacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcgca aagcgcgggc agcaacgtcg tcacgctcgg ctggtttcac gtgatgccgc agcaaagcag cacgccgctt acgacgaatg tcgcgccgac gccgatcaac acgccgctgc gtttgccgcc gtcgttcacg tcggcgggca ccgggctttc gacgagcggc gcggacaccg tcggcctgac cgtcagccac tttctgaccg accacatcgc ggttacctcg gtggcgggcg tgccgcccgt cttcaaggtg tccggccacg gcacgatcac gccgcccggc ccggccggcg cgctcggcac gcagaacatc gggctcgccc aggtcaatcc gatcgtgaag agcgtgcggc agtggagccc cgccgtcgta ttccagtact acttcgcgca agccaccgcg aagttccggc cgttcgtcgg catcggcgtg tcgtacaact ggntcagcga tctgcaactg aacacgaact tcatcaagca gacgcnnncn acntcggcgc gattctcgng cgggcncngg gccggggnca cgcaggnntc nncnannnnt cgtcntcntg ncagctcang nngnnntgnc gnnnanntcn ncnatcntng ggnnngtcnn ntngnncgtn cnnncnctna nnnnnnnnnn nacgnnnacn ntnnnnnnna nnnncnnnnn nnnnnnannn gnnntgnnnn atcnancnnn nnnnnnnnnt nnnnnaaann nnnnnnnana ntnnncnnnn nnnnnnnnnn nnnannnnnn nnnnnnnnnn nncnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAQSAGSNV VTLGWFHVMP QQSSTPLTTN VAPTPINTPL RLPPSFTSAG TGLSTSGADT VGLTVSHFLT DHIAVTSVAG VPPVFKVSGH GTITPPGPAG ALGTQNIGLA QVNPIVKSVR QWSPAVVFQY YFAQATAKFR PFVGIGVSYN WFSDLQLNTN FIKQTQDNLG AILAAGAGKP GVTQVSAKAS SSWQPVFNAG LSYNITDHWG LVASVTYIPL KTTSTVTIKA ADGTTLAESK SDLKADPIIS YVAVSYKF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcaaagc gcgggcagca acgtcgtcac gctcggctgg tttcacgtga tgccgcagca aagcagcacg ccgcttacga cgaatgtcgc gccgacgccg atcaacacgc cgctgcgttt gccgccgtcg ttcacgtcgg cgggcaccgg gctttcgacg agcggcgcgg acaccgtcgg cctgaccgtc agccactttc tgaccgacca catcgcggtt acctcggtgg cgggcgtgcc gcccgtcttc aaggtgtccg gccacggcac gatcacgccg cccggcccgg ccggcgcgct cggcacgcag aacatcgggc tcgcccaggt caatccgatc gtgaagagcg tgcggcagtg gagccccgcc gtcgtattcc agtactactt cgcgcaagcc accgcgaagt tccggccgtt cgtcggcatc ggcgtgtcgt acaactggtt cagcgatctg caactgaaca cgaacttcat caagcagacg caggacaacc tcggcgcgat tctcgccgcg ggcgcgggca agccgggggt cacgcaggta tcggcgaagg cgtcgtcgtc gtggcagccg gtgttcaatg cgggcctgtc gtacaacatc accgatcatt gggggctcgt cgcatcggtc acgtacatcc cgctgaagac gacgtcgacg gtgacgatca aggcggccga cggcacgacg ctcgccgagt cgaaatcgga tctgaaggcc gatccgatca tcagctacgt ggcggtgtcg tacaagttct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa