BupsA.17613.a

CpsI

CENTER ID: BupsA.17613.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17613.a.A1.GE31469 Full length( BupsA.17613.a ) 1 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3402
RefSeq: YP_334636.1
UniProt: Q3JP67

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIVQVLESS ATGTLSMVCL IANRLAREGH DVHVVYSLRP DTPANFHALF DATVKLRHIQ MKGVPLLPML LELRRTLVGI GPDVVHLHSS FAGFLGRIGT LFALREAAFF YSPHCISFMR RDISAFKRAA FVALERIACA KRCLYVACSD SERDAVRVHL RQPVVVVENA VGAMPAPAAE PDGARETVLR IVTVGGIRVQ KNPRLFAEIA RRLCGSRVRF AWIGDGDAAL KAELRDAGVE VAGWLGRGEV VARLRRTDVY LSTSSWEGMP VSVIEAMLLG LPVVASACAG NVDVVRHLQT GAIFRGADDA VELLASLERD AALRARLAHA ARQEARERFG EARFFRQLAR VYASRPARVS
NT Sequence
ATGAAGATCG TGCAGGTCCT CGAATCGAGC GCGACGGGCA CGCTGTCGAT GGTATGCCTG ATCGCGAACC GCCTCGCGCG CGAAGGGCAC GACGTGCACG TCGTCTATTC GCTGCGCCCC GACACGCCCG CGAACTTTCA CGCGTTGTTC GACGCGACGG TCAAGCTGCG CCATATCCAG ATGAAGGGTG TGCCGCTGCT GCCGATGTTG CTTGAATTGC GCCGCACGTT GGTCGGCATC GGGCCGGACG TCGTGCATCT GCATTCATCG TTTGCGGGAT TCCTCGGCAG AATCGGAACG CTGTTCGCGT TGCGCGAAGC GGCGTTCTTC TATAGTCCGC ATTGCATCTC GTTCATGCGG CGCGACATTT CGGCGTTCAA GCGAGCGGCG TTCGTCGCGC TCGAAAGGAT CGCTTGCGCG AAGCGCTGCC TCTACGTTGC GTGCTCGGAC AGCGAGCGTG ACGCGGTGCG CGTGCACCTG CGCCAGCCGG TCGTCGTGGT CGAGAACGCG GTGGGCGCGA TGCCCGCGCC CGCCGCCGAG CCCGACGGCG CGCGCGAGAC GGTGCTGCGG ATCGTGACGG TCGGCGGCAT TCGCGTGCAG AAGAATCCGC GGCTCTTCGC CGAAATCGCG CGCCGCCTGT GCGGCTCGCG TGTGCGCTTC GCGTGGATCG GCGACGGCGA TGCCGCGCTC AAGGCGGAGT TGCGCGACGC CGGCGTCGAG GTTGCCGGTT GGCTCGGCCG CGGCGAAGTC GTCGCGCGAT TGCGGCGCAC GGACGTATAT TTATCGACGT CGTCTTGGGA GGGCATGCCC GTGTCGGTCA TCGAGGCGAT GCTGCTCGGC TTGCCCGTCG TCGCGTCGGC GTGCGCGGGA AACGTCGACG TCGTACGGCA CTTGCAGACC GGCGCGATCT TTCGCGGTGC CGACGACGCG GTCGAATTGC TCGCGTCGCT CGAGCGCGAC GCGGCGCTGC GCGCGCGGCT CGCGCACGCG GCGAGGCAGG AGGCGCGCGA GCGTTTCGGC GAGGCGCGCT TCTTCCGTCA ACTGGCGCGG GTTTATGCGT CGCGGCCCGC GCGCGTATCT
Details for BupsA.17613.a.A1.GE31469
HARVESTED ON: 2/1/2011
SEQUENCED ON: 2/8/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 49
PERCENT COVERAGE: 76
Validated AA Sequence
MXXXXPXXXX HHHHMGTLEA QTQGPGSMKI VQVLESSATG TLSMVCLIAN RLAREGHDVH VVYSLRPDTP ANFHALFDAT VKLRHIQMKG VPLLPMLLEL RRTLVGIGPD VVHLHSSFAG FLGRIGTLFA LREAAFFYSP HCISFMRRXX XXXQASXXSX RXXXXXXXXX XTXXXXXXXX XXXXXXXXXX XXXXXXXXXX XPXXXXXPXX XXXXXXXXXX XXXXXXKXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ccnnnnnnnn nnnnnnnnnn ntgcggnnna gccatgtnnn nnnnnntncc ttgnnnnnnn naccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaag atcgtgcagg tcctcgaatc gagcgcgacg ggcacgctgt cgatggtatg cctgatcgcg aaccgcctcg cgcgcgaagg gcacgacgtg cacgtcgtct attcgctgcg ccccgacacg cccgcgaact ttcacgcgtt gttcgacgcg acggtcaagc tgcgccatat ccagatgaag ggtgtgccgc tgctgccgat gttgcttgaa ttgcgccgca cgttggtcgg catcgggccg gacgtcgtgc atctgcattc atcgtttgcg ggattcctcg gcagaatcgg aacgctgttc gcgttgcgcg aagcggcgtt cttctatagt ccgcattgca tctcgttcat gcggcgcgnc ntttncngcn ttcaagcnag cngnnnttcn ncncgcnngn aangntnnnt tgnnnnaann nntnncacnt tnnnnnntnn nnnnnnnnnn nnnnnnngnn gngnnnnnnc nnnnnccann nnnnnnnntg nnnnanaann cngnnnggnn nnnngcccnn nnncccnncn nannccnnnn nnnnnnnnnn nnnnggnnnn nnnnnnnngn ngnnnnnnnn nnnnnnnnna aaaacnnnnn nnnttnnnnn naannnnnnn nnnnnnnnnn nnnnnntnnn nnnnnnnnnn nnnnnnnncn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntnnnnnnn nnnnnnnnan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKIVQVLES SATGTLSMVC LIANRLAREG HDVHVVYSLR PDTPANFHAL FDATVKLRHI QMKGVPLLPM LLELRRTLVG IGPDVVHLHS SFAGFLGRIG TLFALREAAF FYSPHCISFM RRDISAFKRA AFVALERIAC AKRCLYVACS DSERDAVRVH LRQPVVVVEN AVGAMPAPAA EPDGARETVL RIVTVGGIRV QKNPRLFAEI ARRLCGSRVR FAWIGDGDAA LKAELRDAGV EVAGWLGRGE VVARLRRTDV YLSTSSWEGM PVSVIEAMLL GLPVVASACA GNVDVVRHLQ TGAIFRGADD AVELLASLER DAALRARLAH AARQEARERF GEARFFRQLA RVYASRPARV S
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagatcgtg caggtcctcg aatcgagcgc gacgggcacg ctgtcgatgg tatgcctgat cgcgaaccgc ctcgcgcgcg aagggcacga cgtgcacgtc gtctattcgc tgcgccccga cacgcccgcg aactttcacg cgttgttcga cgcgacggtc aagctgcgcc atatccagat gaagggtgtg ccgctgctgc cgatgttgct tgaattgcgc cgcacgttgg tcggcatcgg gccggacgtc gtgcatctgc attcatcgtt tgcgggattc ctcggcagaa tcggaacgct gttcgcgttg cgcgaagcgg cgttcttcta tagtccgcat tgcatctcgt tcatgcggcg cgacatttcg gcgttcaagc gagcggcgtt cgtcgcgctc gaaaggatcg cttgcgcgaa gcgctgcctc tacgttgcgt gctcggacag cgagcgtgac gcggtgcgcg tgcacctgcg ccagccggtc gtcgtggtcg agaacgcggt gggcgcgatg cccgcgcccg ccgccgagcc cgacggcgcg cgcgagacgg tgctgcggat cgtgacggtc ggcggcattc gcgtgcagaa gaatccgcgg ctcttcgccg aaatcgcgcg ccgcctgtgc ggctcgcgtg tgcgcttcgc gtggatcggc gacggcgatg ccgcgctcaa ggcggagttg cgcgacgccg gcgtcgaggt tgccggttgg ctcggccgcg gcgaagtcgt cgcgcgattg cggcgcacgg acgtatattt atcgacgtcg tcttgggagg gcatgcccgt gtcggtcatc gaggcgatgc tgctcggctt gcccgtcgtc gcgtcggcgt gcgcgggaaa cgtcgacgtc gtacggcact tgcagaccgg cgcgatcttt cgcggtgccg acgacgcggt cgaattgctc gcgtcgctcg agcgcgacgc ggcgctgcgc gcgcggctcg cgcacgcggc gaggcaggag gcgcgcgagc gtttcggcga ggcgcgcttc ttccgtcaac tggcgcgggt ttatgcgtcg cggcccgcgc gcgtatctta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a