BupsA.17601.a

Nitroreductase family protein

CENTER ID: BupsA.17601.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17601.a.A1.GE31461 Full length( BupsA.17601.a ) 1 201
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17601.a.A1.PW31359 Full length( BupsA.17601.a ) 1 201

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3757
RefSeq: YP_334977.1
UniProt: Q3JN76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVKPAPTSV SIHELIAGRW SPRAYSSEPV SAEHLHAVLE AARWAPSAYN AQPWRFIVFD RSKDEVAFKR AFSTLVPFNQ GWNAPAPVLI AVTAHTLTSK GEPSPTALYD AGAAAMSLVL QAHALGLAAH QMSGFDVKAF RAAFAIPADV EPLAIISIGH YGDADKLDPV LRERERAVRT RHPIGEVVYA HAWQKPFPSA D
NT Sequence
ATGTCCGTCA AACCCGCTCC CACTTCCGTT TCGATTCACG AACTGATCGC CGGCCGCTGG AGCCCGCGCG CGTATTCGAG CGAGCCCGTT AGCGCCGAGC ATCTGCACGC CGTGCTCGAA GCGGCGCGCT GGGCGCCGTC CGCGTACAAC GCGCAACCGT GGCGCTTCAT CGTATTCGAC CGCAGCAAGG ACGAGGTCGC GTTCAAGCGC GCATTCTCGA CGCTCGTGCC GTTCAACCAG GGCTGGAACG CACCGGCGCC CGTGCTGATC GCGGTCACCG CGCACACGCT CACGTCGAAG GGCGAGCCGA GTCCGACGGC GCTCTACGAC GCCGGCGCGG CCGCGATGTC GCTCGTGTTG CAAGCGCACG CGCTCGGTCT CGCCGCGCAC CAGATGAGCG GTTTCGACGT GAAGGCGTTC CGCGCCGCAT TCGCGATTCC CGCCGACGTC GAGCCGCTCG CGATCATCTC GATCGGCCAT TACGGCGATG CGGACAAACT CGATCCGGTG CTGCGCGAGC GCGAACGCGC GGTGCGCACG CGCCATCCGA TCGGTGAGGT CGTCTACGCG CACGCGTGGC AGAAGCCGTT CCCGTCGGCA GAT
Details for BupsA.17601.a.A1.GE31461
HARVESTED ON: 2/1/2011
SEQUENCED ON: 2/8/2011
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 19
PERCENT COVERAGE: 80
Validated AA Sequence
MARAHRAFAL AQHRIEFVRI AVMADRDDRE RLDVGGNREC GAERLHVETA HLVRGETERV RLQHERHRGR AGVVERRRTR LALRRERVRG DRDQHGRRCV PALVERHERR ECALERDLVL AAVEYDEAPR LRVVRGRRPA RRFEHGVQML GANGLARIRA RAPAAGDQFV NRNGSGERV
Validated NT Sequence
ttattaatct gccgacggga acggcttctg ccacgcgtgc gcgtagacga cctcaccgat cggatggcgc gtgcgcaccg cgcgttcgcg ctcgcgcagc accggatcga gtttgtccgc atcgccgtaa tggccgatcg agatgatcgc gagcggctcg acgtcggcgg gaatcgcgaa tgcggcgcgg aacgccttca cgtcgaaacc gctcatctgg tgcgcggcga gaccgagcgc gtgcgcttgc aacacgagcg acatcgcggc cgcgccggcg tcgtagagcg ccgtcggact cggctcgccc ttcgacgtga gcgtgtgcgc ggtgaccgcg atcagcacgg gcgccggtgc gttccagccc tggttgaacg gcacgagcgt cgagaatgcg cgcttgaacg cgacctcgtc cttgctgcgg tcgaatacga tgaagcgcca cggttgcgcg ttgtacgcgg acggcgccca gcgcgccgct tcgagcacgg cgtgcagatg ctcggcgcta acgggctcgc tcgaatacgc gcgcgggctc cagcggccgg cgatcagttc gtgaatcgaa acggaagtgg ggagcgggtt tgacggacat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagn cnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVKPAPTS VSIHELIAGR WSPRAYSSEP VSAEHLHAVL EAARWAPSAY NAQPWRFIVF DRSKDEVAFK RAFSTLVPFN QGWNAPAPVL IAVTAHTLTS KGEPSPTALY DAGAAAMSLV LQAHALGLAA HQMSGFDVKA FRAAFAIPAD VEPLAIISIG HYGDADKLDP VLRERERAVR TRHPIGEVVY AHAWQKPFPS AD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgtcaaa cccgctccca cttccgtttc gattcacgaa ctgatcgccg gccgctggag cccgcgcgcg tattcgagcg agcccgttag cgccgagcat ctgcacgccg tgctcgaagc ggcgcgctgg gcgccgtccg cgtacaacgc gcaaccgtgg cgcttcatcg tattcgaccg cagcaaggac gaggtcgcgt tcaagcgcgc attctcgacg ctcgtgccgt tcaaccaggg ctggaacgca ccggcgcccg tgctgatcgc ggtcaccgcg cacacgctca cgtcgaaggg cgagccgagt ccgacggcgc tctacgacgc cggcgcggcc gcgatgtcgc tcgtgttgca agcgcacgcg ctcggtctcg ccgcgcacca gatgagcggt ttcgacgtga aggcgttccg cgccgcattc gcgattcccg ccgacgtcga gccgctcgcg atcatctcga tcggccatta cggcgatgcg gacaaactcg atccggtgct gcgcgagcgc gaacgcgcgg tgcgcacgcg ccatccgatc ggtgaggtcg tctacgcgca cgcgtggcag aagccgttcc cgtcggcaga ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BupsA.17601.a.A1.PW31359
PURIFICATION DATe: 4/15/2011
CONCENTRATION: 23.01mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 19
PERCENT COVERAGE: 80
Protocol Notes
notes unavailable
Validated AA Sequence
MARAHRAFAL AQHRIEFVRI AVMADRDDRE RLDVGGNREC GAERLHVETA HLVRGETERV RLQHERHRGR AGVVERRRTR LALRRERVRG DRDQHGRRCV PALVERHERR ECALERDLVL AAVEYDEAPR LRVVRGRRPA RRFEHGVQML GANGLARIRA RAPAAGDQFV NRNGSGERV
Validated NT Sequence
ttattaatct gccgacggga acggcttctg ccacgcgtgc gcgtagacga cctcaccgat cggatggcgc gtgcgcaccg cgcgttcgcg ctcgcgcagc accggatcga gtttgtccgc atcgccgtaa tggccgatcg agatgatcgc gagcggctcg acgtcggcgg gaatcgcgaa tgcggcgcgg aacgccttca cgtcgaaacc gctcatctgg tgcgcggcga gaccgagcgc gtgcgcttgc aacacgagcg acatcgcggc cgcgccggcg tcgtagagcg ccgtcggact cggctcgccc ttcgacgtga gcgtgtgcgc ggtgaccgcg atcagcacgg gcgccggtgc gttccagccc tggttgaacg gcacgagcgt cgagaatgcg cgcttgaacg cgacctcgtc cttgctgcgg tcgaatacga tgaagcgcca cggttgcgcg ttgtacgcgg acggcgccca gcgcgccgct tcgagcacgg cgtgcagatg ctcggcgcta acgggctcgc tcgaatacgc gcgcgggctc cagcggccgg cgatcagttc gtgaatcgaa acggaagtgg ggagcgggtt tgacggacat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagn cnt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVKPAPTS VSIHELIAGR WSPRAYSSEP VSAEHLHAVL EAARWAPSAY NAQPWRFIVF DRSKDEVAFK RAFSTLVPFN QGWNAPAPVL IAVTAHTLTS KGEPSPTALY DAGAAAMSLV LQAHALGLAA HQMSGFDVKA FRAAFAIPAD VEPLAIISIG HYGDADKLDP VLRERERAVR TRHPIGEVVY AHAWQKPFPS AD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCGTCAAA CCCGCTCCCA CTTCCGTTTC GATTCACGAA CTGATCGCCG GCCGCTGGAG CCCGCGCGCG TATTCGAGCG AGCCCGTTAG CGCCGAGCAT CTGCACGCCG TGCTCGAAGC GGCGCGCTGG GCGCCGTCCG CGTACAACGC GCAACCGTGG CGCTTCATCG TATTCGACCG CAGCAAGGAC GAGGTCGCGT TCAAGCGCGC ATTCTCGACG CTCGTGCCGT TCAACCAGGG CTGGAACGCA CCGGCGCCCG TGCTGATCGC GGTCACCGCG CACACGCTCA CGTCGAAGGG CGAGCCGAGT CCGACGGCGC TCTACGACGC CGGCGCGGCC GCGATGTCGC TCGTGTTGCA AGCGCACGCG CTCGGTCTCG CCGCGCACCA GATGAGCGGT TTCGACGTGA AGGCGTTCCG CGCCGCATTC GCGATTCCCG CCGACGTCGA GCCGCTCGCG ATCATCTCGA TCGGCCATTA CGGCGATGCG GACAAACTCG ATCCGGTGCT GCGCGAGCGC GAACGCGCGG TGCGCACGCG CCATCCGATC GGTGAGGTCG TCTACGCGCA CGCGTGGCAG AAGCCGTTCC CGTCGGCAGA TTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA