BupsA.17580.a

Putative uncharacterized protein

CENTER ID: BupsA.17580.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17580.a.A1.GE31352 Full length( BupsA.17580.a ) 1 200
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17580.a.A1.PS01026 Full length( BupsA.17580.a ) 1 200

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.1273
RefSeq: YP_332675.1
UniProt: Q3JUS8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPEPGVGAHA GGLDADAALA RFFVITGGPG SGKSTLIDAL EARGYARSHE AGRGVIQDQV AIGGHALPWR DRAAFADMML GWEMRSHHLA RRARGPVFFD RGVPDVIGYL KLSALPVPAH LVAAAERFRY RRDVFIAPPW PEIYAQDAER RQDYAEAVRT YGAMVDTYAA CGYRLIELPR ASVDERCRFV LDVVEAAAAV
NT Sequence
ATGCCTGAGC CGGGCGTCGG CGCGCACGCG GGCGGGCTCG ACGCCGATGC GGCGCTCGCC CGCTTCTTCG TGATCACGGG CGGGCCCGGC TCGGGCAAGA GCACGCTGAT CGACGCGCTC GAGGCGCGCG GTTACGCGCG CTCGCACGAA GCCGGGCGCG GCGTGATTCA GGATCAGGTG GCGATCGGCG GTCACGCGCT GCCGTGGCGG GACCGCGCCG CGTTCGCCGA CATGATGCTC GGCTGGGAGA TGCGCTCGCA CCACCTCGCA CGGCGCGCGC GCGGGCCGGT GTTCTTCGAT CGCGGCGTGC CCGACGTGAT CGGCTATCTG AAACTGTCCG CGCTGCCCGT GCCCGCGCAT CTCGTCGCGG CCGCCGAGCG GTTCCGCTAT CGCCGCGACG TGTTCATCGC GCCGCCGTGG CCGGAGATCT ACGCGCAGGA TGCCGAACGC AGGCAGGATT ACGCCGAGGC CGTGCGTACC TACGGCGCGA TGGTCGACAC GTATGCGGCG TGCGGCTACC GGCTGATCGA ACTGCCGCGC GCGAGCGTCG ACGAGCGTTG CCGTTTCGTG CTCGATGTCG TCGAGGCAGC CGCGGCGGTG
Details for BupsA.17580.a.A1.GE31352
HARVESTED ON: 1/26/2011
SEQUENCED ON: 1/31/2011
EXPECTED MW: 24kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEPGVGAH AGGLDADAAL ARFFVITGGP GSGKSTLIDA LEARGYARSH EAGRGVIQDQ VAIGGHALPW RDRAAFADMM LGWEMRSHHL ARRARGPVFF DRGVPDVIGY LKLSALPVPA HLVAAAERFR YRRDVFIAPP WPEIYAQDAE RRQDYAEAVR TYGAMVDTYA ACGYRLIELP RASVDERCRF VLDVVEAAAA V
Validated NT Sequence
ttattacacc gccgcggctg cctcgacgac atcgagcacg aaacggcaac gctcgtcgac gctcgcgcgc ggcagttcga tcagccggta gccgcacgcc gcatacgtgt cgaccatcgc gccgtaggta cgcacggcct cggcgtaatc ctgcctgcgt tcggcatcct gcgcgtagat ctccggccac ggcggcgcga tgaacacgtc gcggcgatag cggaaccgct cggcggccgc gacgagatgc gcgggcacgg gcagcgcgga cagtttcaga tagccgatca cgtcgggcac gccgcgatcg aagaacaccg gcccgcgcgc gcgccgtgcg aggtggtgcg agcgcatctc ccagccgagc atcatgtcgg cgaacgcggc gcggtcccgc cacggcagcg cgtgaccgcc gatcgccacc tgatcctgaa tcacgccgcg cccggcttcg tgcgagcgcg cgtaaccgcg cgcctcgagc gcgtcgatca gcgtgctctt gcccgagccg ggcccgcccg tgatcacgaa gaagcgggcg agcgccgcat cggcgtcgag cccgcccgcg tgcgcgccga cgcccggctc aggcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEPGVGAH AGGLDADAAL ARFFVITGGP GSGKSTLIDA LEARGYARSH EAGRGVIQDQ VAIGGHALPW RDRAAFADMM LGWEMRSHHL ARRARGPVFF DRGVPDVIGY LKLSALPVPA HLVAAAERFR YRRDVFIAPP WPEIYAQDAE RRQDYAEAVR TYGAMVDTYA ACGYRLIELP RASVDERCRF VLDVVEAAAA V
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctgagccg ggcgtcggcg cgcacgcggg cgggctcgac gccgatgcgg cgctcgcccg cttcttcgtg atcacgggcg ggcccggctc gggcaagagc acgctgatcg acgcgctcga ggcgcgcggt tacgcgcgct cgcacgaagc cgggcgcggc gtgattcagg atcaggtggc gatcggcggt cacgcgctgc cgtggcggga ccgcgccgcg ttcgccgaca tgatgctcgg ctgggagatg cgctcgcacc acctcgcacg gcgcgcgcgc gggccggtgt tcttcgatcg cggcgtgccc gacgtgatcg gctatctgaa actgtccgcg ctgcccgtgc ccgcgcatct cgtcgcggcc gccgagcggt tccgctatcg ccgcgacgtg ttcatcgcgc cgccgtggcc ggagatctac gcgcaggatg ccgaacgcag gcaggattac gccgaggccg tgcgtaccta cggcgcgatg gtcgacacgt atgcggcgtg cggctaccgg ctgatcgaac tgccgcgcgc gagcgtcgac gagcgttgcc gtttcgtgct cgatgtcgtc gaggcagccg cggcggtgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BupsA.17580.a.A1.PS01026
PURIFICATION DATe: 4/6/2011
CONCENTRATION: 8.58mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEPGVGAH AGGLDADAAL ARFFVITGGP GSGKSTLIDA LEARGYARSH EAGRGVIQDQ VAIGGHALPW RDRAAFADMM LGWEMRSHHL ARRARGPVFF DRGVPDVIGY LKLSALPVPA HLVAAAERFR YRRDVFIAPP WPEIYAQDAE RRQDYAEAVR TYGAMVDTYA ACGYRLIELP RASVDERCRF VLDVVEAAAA V
Validated NT Sequence
ttattacacc gccgcggctg cctcgacgac atcgagcacg aaacggcaac gctcgtcgac gctcgcgcgc ggcagttcga tcagccggta gccgcacgcc gcatacgtgt cgaccatcgc gccgtaggta cgcacggcct cggcgtaatc ctgcctgcgt tcggcatcct gcgcgtagat ctccggccac ggcggcgcga tgaacacgtc gcggcgatag cggaaccgct cggcggccgc gacgagatgc gcgggcacgg gcagcgcgga cagtttcaga tagccgatca cgtcgggcac gccgcgatcg aagaacaccg gcccgcgcgc gcgccgtgcg aggtggtgcg agcgcatctc ccagccgagc atcatgtcgg cgaacgcggc gcggtcccgc cacggcagcg cgtgaccgcc gatcgccacc tgatcctgaa tcacgccgcg cccggcttcg tgcgagcgcg cgtaaccgcg cgcctcgagc gcgtcgatca gcgtgctctt gcccgagccg ggcccgcccg tgatcacgaa gaagcgggcg agcgccgcat cggcgtcgag cccgcccgcg tgcgcgccga cgcccggctc aggcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEPGVGAH AGGLDADAAL ARFFVITGGP GSGKSTLIDA LEARGYARSH EAGRGVIQDQ VAIGGHALPW RDRAAFADMM LGWEMRSHHL ARRARGPVFF DRGVPDVIGY LKLSALPVPA HLVAAAERFR YRRDVFIAPP WPEIYAQDAE RRQDYAEAVR TYGAMVDTYA ACGYRLIELP RASVDERCRF VLDVVEAAAA V
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTGAGCCG GGCGTCGGCG CGCACGCGGG CGGGCTCGAC GCCGATGCGG CGCTCGCCCG CTTCTTCGTG ATCACGGGCG GGCCCGGCTC GGGCAAGAGC ACGCTGATCG ACGCGCTCGA GGCGCGCGGT TACGCGCGCT CGCACGAAGC CGGGCGCGGC GTGATTCAGG ATCAGGTGGC GATCGGCGGT CACGCGCTGC CGTGGCGGGA CCGCGCCGCG TTCGCCGACA TGATGCTCGG CTGGGAGATG CGCTCGCACC ACCTCGCACG GCGCGCGCGC GGGCCGGTGT TCTTCGATCG CGGCGTGCCC GACGTGATCG GCTATCTGAA ACTGTCCGCG CTGCCCGTGC CCGCGCATCT CGTCGCGGCC GCCGAGCGGT TCCGCTATCG CCGCGACGTG TTCATCGCGC CGCCGTGGCC GGAGATCTAC GCGCAGGATG CCGAACGCAG GCAGGATTAC GCCGAGGCCG TGCGTACCTA CGGCGCGATG GTCGACACGT ATGCGGCGTG CGGCTACCGG CTGATCGAAC TGCCGCGCGC GAGCGTCGAC GAGCGTTGCC GTTTCGTGCT CGATGTCGTC GAGGCAGCCG CGGCGGTGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A