BupsA.17570.a

Chlorohydrolase family protein

CENTER ID: BupsA.17570.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17570.a.A1.GE31346 Full length( BupsA.17570.a ) 1 476
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2646
RefSeq: YP_333918.1
UniProt: Q3JR85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MERHPSARAG AHSLSQPPSL SPNRSKTLVV KHADVLVTMD GARRELRDAG LYVEDNRIVA VGPSAELPEQ ADEVLDLRGH LVIPGLVNTH HHMYQSLTRA IPAAQNAELF GWLTNLYRIW AHLTPEMIEV SALTAMAELL LSGCTTSSDH LYIYPNGSRL DDSIAAARRI GMRFHASRGS MSVGQRDGGL PPDAVVEREA DILRDTQRVI ETYHDEGRYA MLRIAVAPCS PFSVSRGLMR DAAALAREHR VSLHTHLAEN VNDVAYSREK FGMTPAEYAE DLGWVGRDVW HAHCVRLDEP GIALFARTGT GVAHCPCSNM RLASGIAPIA RMRRAGVPVG LGVDGCASND GAQMVAEARQ ALLLQRVGFG PDALSARDAL EIATLGGARV LNRDDIGALA PGMAADFVAF DLRTPQFAGA LHDPVAALVF CAPPQAAYSV VNGRVVVREG RLTTLEIEPL VERHNALARA LCDAAR
NT Sequence
ATGGAACGAC ACCCGAGCGC GCGAGCCGGC GCGCACTCCC TATCCCAGCC CCCCTCCCTT TCCCCGAACC GATCGAAGAC GCTCGTCGTC AAGCACGCCG ACGTGCTCGT GACGATGGAC GGCGCGCGCC GCGAACTGCG CGATGCGGGC CTGTATGTCG AGGACAACCG GATCGTCGCG GTCGGCCCGA GCGCCGAGTT GCCCGAGCAG GCGGACGAAG TGCTCGATCT GCGCGGGCAT CTCGTGATCC CGGGGCTCGT CAACACGCAT CATCATATGT ATCAGAGCCT CACGCGCGCG ATTCCCGCCG CGCAGAACGC CGAGCTGTTC GGCTGGCTCA CGAATCTATA CCGGATCTGG GCGCATCTGA CGCCGGAGAT GATCGAGGTA TCGGCGCTGA CCGCGATGGC CGAGCTGCTG CTGTCCGGCT GCACGACGTC GAGCGATCAT CTGTACATCT ATCCGAACGG CAGCCGGCTC GACGACAGCA TCGCGGCCGC GCGGCGCATC GGCATGCGCT TTCACGCGAG CCGCGGCAGC ATGAGCGTCG GGCAGCGCGA CGGCGGGTTG CCGCCCGATG CGGTCGTCGA GCGCGAGGCG GACATCCTGC GCGATACGCA GCGCGTGATC GAGACCTACC ATGACGAAGG CCGCTATGCG ATGCTGCGTA TCGCCGTCGC GCCGTGTTCG CCGTTCTCGG TGAGCCGCGG CCTGATGCGC GACGCGGCGG CGCTCGCGCG CGAGCACCGC GTGTCGCTGC ACACGCACCT CGCGGAGAAC GTGAACGACG TCGCGTACAG CCGCGAGAAG TTCGGGATGA CGCCGGCCGA GTATGCGGAG GATCTCGGCT GGGTGGGGCG CGACGTGTGG CACGCGCATT GCGTGCGGCT CGACGAGCCC GGCATCGCGC TTTTTGCGCG CACCGGCACG GGCGTCGCGC ATTGCCCTTG CTCGAACATG CGGCTGGCGT CCGGGATCGC CCCCATCGCG CGAATGCGGC GCGCGGGCGT GCCGGTCGGG CTCGGCGTCG ACGGTTGCGC GTCGAACGAC GGCGCGCAGA TGGTGGCCGA GGCGCGGCAG GCGCTGCTGC TGCAGCGCGT CGGATTCGGG CCGGACGCGC TGAGCGCGCG CGACGCGCTC GAGATCGCGA CGCTCGGCGG CGCGCGCGTG CTGAACCGCG ACGACATCGG CGCGCTCGCG CCGGGCATGG CCGCGGATTT CGTCGCGTTC GACCTGCGCA CGCCGCAGTT CGCGGGCGCG CTGCACGATC CCGTCGCGGC GCTCGTGTTC TGCGCACCGC CGCAGGCGGC GTACAGCGTC GTCAACGGGC GCGTCGTCGT GCGGGAAGGG CGGCTGACGA CGCTCGAGAT CGAGCCGCTC GTCGAGCGGC ACAACGCGCT GGCTCGCGCG CTTTGTGACG CGGCGCGC
Details for BupsA.17570.a.A1.GE31346
HARVESTED ON: 1/26/2011
SEQUENCED ON: 1/31/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 34
PERCENT COVERAGE: 10
Validated AA Sequence
MDGARRAARC GPVCRGXPDX XGRXXXXVAR AGGRXXRSAX XXRXXGXXXX XX
Validated NT Sequence
ggnncngccn tgtttnnntn nctcntngnn atccanaann nnnnacaann nnannncann annntnncaa nnnggncntg gntnnanggn ncgacacccg agcgcgcgag ccggcgcgca ctccctatcc cagcccccct ccctttcccc gaanngatcg aagacgctcg ncgtcaagca cgccganntg ntcgtgacga tggacggngc gcgccgngct gcgcgatgcg ggcctgtntg tcgaggacnn ccggatcnnc ncggncggnc ngnnngccna gttgcccgag caggcggacg anntgnncgn tcngcnnggg nntnncgngn nnncggnnnn nnncnacncg nnnngnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMERHPSARA GAHSLSQPPS LSPNRSKTLV VKHADVLVTM DGARRELRDA GLYVEDNRIV AVGPSAELPE QADEVLDLRG HLVIPGLVNT HHHMYQSLTR AIPAAQNAEL FGWLTNLYRI WAHLTPEMIE VSALTAMAEL LLSGCTTSSD HLYIYPNGSR LDDSIAAARR IGMRFHASRG SMSVGQRDGG LPPDAVVERE ADILRDTQRV IETYHDEGRY AMLRIAVAPC SPFSVSRGLM RDAAALAREH RVSLHTHLAE NVNDVAYSRE KFGMTPAEYA EDLGWVGRDV WHAHCVRLDE PGIALFARTG TGVAHCPCSN MRLASGIAPI ARMRRAGVPV GLGVDGCASN DGAQMVAEAR QALLLQRVGF GPDALSARDA LEIATLGGAR VLNRDDIGAL APGMAADFVA FDLRTPQFAG ALHDPVAALV FCAPPQAAYS VVNGRVVVRE GRLTTLEIEP LVERHNALAR ALCDAAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaacgacac ccgagcgcgc gagccggcgc gcactcccta tcccagcccc cctccctttc cccgaaccga tcgaagacgc tcgtcgtcaa gcacgccgac gtgctcgtga cgatggacgg cgcgcgccgc gaactgcgcg atgcgggcct gtatgtcgag gacaaccgga tcgtcgcggt cggcccgagc gccgagttgc ccgagcaggc ggacgaagtg ctcgatctgc gcgggcatct cgtgatcccg gggctcgtca acacgcatca tcatatgtat cagagcctca cgcgcgcgat tcccgccgcg cagaacgccg agctgttcgg ctggctcacg aatctatacc ggatctgggc gcatctgacg ccggagatga tcgaggtatc ggcgctgacc gcgatggccg agctgctgct gtccggctgc acgacgtcga gcgatcatct gtacatctat ccgaacggca gccggctcga cgacagcatc gcggccgcgc ggcgcatcgg catgcgcttt cacgcgagcc gcggcagcat gagcgtcggg cagcgcgacg gcgggttgcc gcccgatgcg gtcgtcgagc gcgaggcgga catcctgcgc gatacgcagc gcgtgatcga gacctaccat gacgaaggcc gctatgcgat gctgcgtatc gccgtcgcgc cgtgttcgcc gttctcggtg agccgcggcc tgatgcgcga cgcggcggcg ctcgcgcgcg agcaccgcgt gtcgctgcac acgcacctcg cggagaacgt gaacgacgtc gcgtacagcc gcgagaagtt cgggatgacg ccggccgagt atgcggagga tctcggctgg gtggggcgcg acgtgtggca cgcgcattgc gtgcggctcg acgagcccgg catcgcgctt tttgcgcgca ccggcacggg cgtcgcgcat tgcccttgct cgaacatgcg gctggcgtcc gggatcgccc ccatcgcgcg aatgcggcgc gcgggcgtgc cggtcgggct cggcgtcgac ggttgcgcgt cgaacgacgg cgcgcagatg gtggccgagg cgcggcaggc gctgctgctg cagcgcgtcg gattcgggcc ggacgcgctg agcgcgcgcg acgcgctcga gatcgcgacg ctcggcggcg cgcgcgtgct gaaccgcgac gacatcggcg cgctcgcgcc gggcatggcc gcggatttcg tcgcgttcga cctgcgcacg ccgcagttcg cgggcgcgct gcacgatccc gtcgcggcgc tcgtgttctg cgcaccgccg caggcggcgt acagcgtcgt caacgggcgc gtcgtcgtgc gggaagggcg gctgacgacg ctcgagatcg agccgctcgt cgagcggcac aacgcgctgg ctcgcgcgct ttgtgacgcg gcgcgctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa