BupsA.17564.a

ATPase, AAA family protein

CENTER ID: BupsA.17564.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17564.a.A1.GE31342 Full length( BupsA.17564.a ) 1 317
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.1352
RefSeq: YP_332741.1
UniProt: Q3JUL2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPPCGPAEH APHDTPAVRG RRRFCLILGF FRRKLAAMRF EGSSHYVATD DLKLAVNAAL TLERPLLIKG EPGTGKTMLA EEVAAALGMP LLQWHIKSTT KAQQGLYEYD AVSRLRDSQL GDERVKDIAN YIVKGVLWQA FEAQHPSVLL IDEIDKADIE FPNDLLRELD RMEFHVYETR ETVRARHRPL VIITSNNEKE LPDAFLRRCF FHYIQFPDPS TMQKIVEVHY PGIKQDLLRA ALESFFELRG VSGLKKKPST SELLDWLKLL LAEDIPPDAL RSKDQKQIVP PLAGALLKNE QDVSLFERLV YMNRHNR
NT Sequence
ATGCGTCCAC CATGCGGCCC TGCCGAGCAC GCCCCGCACG ATACACCCGC CGTGCGAGGC CGGCGGCGCT TCTGCCTTAT ACTGGGATTT TTTCGCCGGA AGCTCGCCGC CATGCGTTTC GAAGGTTCCT CGCACTACGT CGCGACAGAC GATCTGAAGC TCGCCGTCAA CGCCGCGCTC ACGCTCGAGC GCCCGCTCCT CATCAAGGGC GAGCCCGGCA CGGGCAAGAC CATGCTCGCC GAGGAAGTCG CGGCGGCGCT CGGCATGCCG CTGCTGCAAT GGCACATCAA ATCGACGACG AAGGCGCAGC AGGGGCTCTA CGAATACGAT GCGGTGTCGC GCCTGCGCGA CTCGCAGCTC GGCGACGAGC GCGTGAAGGA CATCGCGAAC TACATCGTGA AGGGCGTGCT GTGGCAGGCG TTCGAAGCGC AGCACCCGAG CGTGCTGCTC ATCGACGAGA TCGACAAGGC CGACATCGAA TTCCCGAACG ATCTGCTGCG CGAGCTCGAC CGGATGGAGT TCCACGTGTA CGAGACGCGC GAAACCGTGC GCGCGCGGCA CCGCCCGCTC GTCATCATCA CGTCGAACAA CGAGAAGGAG CTGCCCGACG CGTTCCTGCG CCGCTGTTTC TTCCACTACA TCCAGTTCCC CGATCCGTCG ACGATGCAGA AGATCGTCGA GGTCCACTAC CCCGGCATCA AGCAGGACCT GCTGCGCGCG GCGCTCGAGA GCTTCTTCGA GCTGCGCGGC GTATCGGGGC TGAAGAAGAA GCCGTCGACG TCCGAGCTGC TCGACTGGCT GAAGCTGCTG CTCGCCGAGG ACATCCCGCC CGATGCGCTG CGCTCGAAGG ACCAGAAGCA GATCGTGCCG CCGCTTGCCG GCGCGCTGCT CAAGAACGAG CAGGACGTGA GCCTGTTCGA GCGGCTCGTC TACATGAACC GCCACAACCG C
Details for BupsA.17564.a.A1.GE31342
HARVESTED ON: 1/26/2011
SEQUENCED ON: 1/31/2011
EXPECTED MW: 38kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 31
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMRPPCGP AEHAPHDTPA VRGRRRFCLI LGFFRRKLAA HAFRRFLALR RDRRSEARRQ RRAHARAPAP HQGRARHGQD HARRGSRGGA RHAAAAMAHQ IDDEGAAGAL RIRCGVAPAR LAARRRAREG HRELHREGRA VAGVRSAAPE RAAHRRDRQG RHRIPERSAA RARPDGVPRV RDARNRARAA PPARHHHVEQ REGAARRVPA PLFLPLHPVP RSVDDAEDRR GPLPRHQAGP AARGARELLR AARRIGAEEE AVDVRAARLA EAAARRGHPA RCAALEGPEA DRAAACRRAA QERAGREPVR AARLHEPPQP
Validated NT Sequence
gcggttgtgg cggttcatgt agacgagccg ctcgaacagg ctcacgtcct gctcgttctt gagcagcgcg ccggcaagcg gcggcacgat ctgcttctgg tccttcgagc gcagcgcatc gggcgggatg tcctcggcga gcagcagctt cagccagtcg agcagctcgg acgtcgacgg cttcttcttc agccccgata cgccgcgcag ctcgaagaag ctctcgagcg ccgcgcgcag caggtcctgc ttgatgccgg ggtagtggac ctcgacgatc ttctgcatcg tcgacggatc ggggaactgg atgtagtgga agaaacagcg gcgcaggaac gcgtcgggca gctccttctc gttgttcgac gtgatgatga cgagcgggcg gtgccgcgcg cgcacggttt cgcgcgtctc gtacacgtgg aactccatcc ggtcgagctc gcgcagcaga tcgttcggga attcgatgtc ggccttgtcg atctcgtcga tgagcagcac gctcgggtgc tgcgcttcga acgcctgcca cagcacgccc ttcacgatgt agttcgcgat gtccttcacg cgctcgtcgc cgagctgcga gtcgcgcagg cgcgacaccg catcgtattc gtagagcccc tgctgcgcct tcgtcgtcga tttgatgtgc cattgcagca gcggcatgcc gagcgccgcc gcgacttcct cggcgagcat ggtcttgccc gtgccgggct cgcccttgat gaggagcggg cgctcgagcg tgagcgcggc gttgacggcg agcttcagat cgtctgtcgc gacgtagtgc gaggaacctt cgaaacgcat gggcggcgag cttccggcga aaaaatccca gtataaggca gaagcgccgc cggcctcgca cggcgggtgt atcgtgcggg gcgtgctcgg cagggccgca tggtggacgc atcgaaccag gaccctgggt ctgagcttcc agggtaccca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRPPCGPAE HAPHDTPAVR GRRRFCLILG FFRRKLAAMR FEGSSHYVAT DDLKLAVNAA LTLERPLLIK GEPGTGKTML AEEVAAALGM PLLQWHIKST TKAQQGLYEY DAVSRLRDSQ LGDERVKDIA NYIVKGVLWQ AFEAQHPSVL LIDEIDKADI EFPNDLLREL DRMEFHVYET RETVRARHRP LVIITSNNEK ELPDAFLRRC FFHYIQFPDP STMQKIVEVH YPGIKQDLLR AALESFFELR GVSGLKKKPS TSELLDWLKL LLAEDIPPDA LRSKDQKQIV PPLAGALLKN EQDVSLFERL VYMNRHNR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtccacca tgcggccctg ccgagcacgc cccgcacgat acacccgccg tgcgaggccg gcggcgcttc tgccttatac tgggattttt tcgccggaag ctcgccgcca tgcgtttcga aggttcctcg cactacgtcg cgacagacga tctgaagctc gccgtcaacg ccgcgctcac gctcgagcgc ccgctcctca tcaagggcga gcccggcacg ggcaagacca tgctcgccga ggaagtcgcg gcggcgctcg gcatgccgct gctgcaatgg cacatcaaat cgacgacgaa ggcgcagcag gggctctacg aatacgatgc ggtgtcgcgc ctgcgcgact cgcagctcgg cgacgagcgc gtgaaggaca tcgcgaacta catcgtgaag ggcgtgctgt ggcaggcgtt cgaagcgcag cacccgagcg tgctgctcat cgacgagatc gacaaggccg acatcgaatt cccgaacgat ctgctgcgcg agctcgaccg gatggagttc cacgtgtacg agacgcgcga aaccgtgcgc gcgcggcacc gcccgctcgt catcatcacg tcgaacaacg agaaggagct gcccgacgcg ttcctgcgcc gctgtttctt ccactacatc cagttccccg atccgtcgac gatgcagaag atcgtcgagg tccactaccc cggcatcaag caggacctgc tgcgcgcggc gctcgagagc ttcttcgagc tgcgcggcgt atcggggctg aagaagaagc cgtcgacgtc cgagctgctc gactggctga agctgctgct cgccgaggac atcccgcccg atgcgctgcg ctcgaaggac cagaagcaga tcgtgccgcc gcttgccggc gcgctgctca agaacgagca ggacgtgagc ctgttcgagc ggctcgtcta catgaaccgc cacaaccgct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa