SSGCID: Target Page

BupsA.17551.a

Segregation and condensation protein B

CENTER ID:	BupsA.17551.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17551.a.A1.GE31336	Full length( BupsA.17551.a )		1	456

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17551.a.A1.PS01141	Full length( BupsA.17551.a )		1	456

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.1994
RefSeq:	YP_333310.1
UniProt:	Q3JSZ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNTQEAKIVL ETALICAQEP LKLGDLRKLF ADGVSADTVR TLLEDLKQEW TGRGVELVAL ASGWRFQSKP AMRTYLDRLH PEKPPKYSRA VLETLAIIAY RQPVTRGDIE EIRGVTVNTQ VVKQLEDRGW IEVIGHRDVP GRPALYATTK QFLDDLGLKA LDDLPALEEP AAHLEASLLA QQAIDFPDDA RGDGEAPAVA PETDAAAAQA GEVAEPASDS VVLRNEDTAR ADEPRQADAA PTRADEPRQE GATDGLAARI GDPADNDAGR ADVDVDAAPA LGEADAPRGG DASGHAAREI DSREMTEPAD AGGEAAGTTA SHTHAVAPYD HDAQSAADAV AAPHAGDVAA DGERAESPSG TNENANYVAQ PDAEPADAAH AATREVAADP AAGRATKEDH GAIGGIPHDA EPVRAHAEEA LDDTSDSLAD AVRSASEAAP EHERQDDEDA PAKRRA

NT Sequence

ATGAATACCC AAGAGGCGAA GATCGTCCTC GAGACTGCCT TGATCTGCGC GCAGGAACCG CTGAAGCTCG GCGATTTGCG CAAGCTCTTT GCCGACGGCG TGTCGGCGGA TACAGTCCGA ACACTGCTCG AAGATCTGAA ACAGGAATGG ACCGGACGGG GGGTCGAACT CGTCGCGCTC GCGTCGGGCT GGCGCTTCCA GAGCAAGCCG GCGATGCGCA CGTATCTCGA TCGCCTGCAT CCCGAGAAGC CGCCGAAGTA TTCGCGCGCC GTGCTGGAGA CGCTCGCGAT CATCGCGTAC CGGCAGCCGG TCACGCGCGG CGACATCGAG GAGATCCGCG GCGTCACGGT GAACACGCAG GTCGTCAAGC AGCTCGAGGA CCGCGGCTGG ATCGAAGTGA TCGGTCATCG CGACGTGCCG GGCCGTCCGG CGCTCTACGC GACGACAAAA CAGTTTCTCG ACGACCTCGG CCTGAAGGCG CTCGACGATC TGCCCGCGCT CGAGGAGCCG GCCGCGCATC TGGAGGCGAG CCTGCTCGCG CAGCAGGCGA TCGATTTCCC CGATGACGCG CGCGGCGACG GCGAAGCGCC TGCCGTCGCG CCCGAGACGG ACGCCGCCGC CGCGCAGGCG GGCGAGGTTG CCGAGCCGGC GAGCGATTCG GTGGTTTTGC GCAACGAAGA TACGGCGCGC GCCGACGAGC CGCGGCAGGC GGACGCGGCG CCGACGCGTG CCGACGAGCC GCGGCAGGAA GGGGCGACGG ACGGCTTGGC GGCACGGATC GGCGATCCAG CCGATAATGA CGCCGGGCGA GCCGATGTGG ACGTCGACGC GGCGCCGGCT CTCGGCGAAG CCGATGCGCC GCGCGGCGGC GATGCTTCAG GGCATGCGGC TCGCGAGATC GACTCGCGCG AGATGACGGA GCCGGCAGAC GCCGGCGGCG AGGCGGCCGG CACGACCGCA TCGCATACGC ATGCGGTCGC GCCGTACGAC CATGATGCGC AGAGCGCAGC GGACGCCGTC GCGGCGCCGC ACGCCGGCGA TGTAGCCGCT GATGGCGAGC GCGCCGAATC GCCCTCGGGG ACGAACGAAA ACGCAAACTA CGTCGCGCAG CCGGACGCGG AGCCGGCCGA CGCGGCACAC GCAGCAACAC GCGAGGTGGC CGCCGACCCG GCGGCGGGCC GAGCGACGAA GGAAGACCAC GGCGCGATCG GCGGCATCCC GCACGATGCC GAGCCGGTCC GGGCGCACGC GGAGGAGGCG TTGGACGACA CGTCCGACAG TCTCGCCGAC GCCGTGCGCA GTGCAAGCGA GGCGGCCCCC GAGCACGAGC GGCAGGACGA CGAGGACGCG CCTGCGAAGC GTCGCGCC

Details for BupsA.17551.a.A1.GE31336

HARVESTED ON:	data unavailable
SEQUENCED ON:	1/31/2011
EXPECTED MW:	50kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	74
PERCENT COVERAGE:	39

Validated AA Sequence

MNTQEAKIVL ETALICAQEP LXXXXXXKLF ADGVSADTVR TLLEDLKQEW TGRGVELVAL ASGWRFQSKP AMRTYLDRLH PEKPPKYSRA VLETLAXXAY RQPVTRGDIX XIRGVTVNTQ XXKXLEDRGW XEVIXHRDXP GRPALYXTTX XFXXXLXXXX LDXLXXXXXX XRAXXXEPLX XRXSIXX

Validated NT Sequence

cnnnnnnnnn nnnnnnnnnn nnnnngcggn nnagccntgt ttnnnnnnct ccnngnnnnn nnnnnnnnna nnnnnnnnng nnnaccnngn nagctcanac ccngggtcct ggttcgatga atacccaaga ggcgaagatc gtcctcgaga ctgccttgat ctgcgcgcag gaaccgctga annnnnnctn nnnnngcaag ctctttgccg acggcgtgtc ggcggataca gtccgaacac tgctcgaaga tctgaaacag gaatggaccg gacggggggt cgaactcgtc gcgctcgcgt cgggctggcg cttccagagc aagccggcga tgcgcacgta tctcgatcgc ctgcatcccg agaagccgcc gaagtattcg cgcgccgtgc tggagacgct cgcnancntc gcgtaccggc agccggtcac gcgcggcgac atcnngnaga tccgcggcgt cacggtgaac acgcaggncn ncaagcanct cgaggaccgc ggctggancg aagtgatcgn ncatcgcgac ntgccgggcc gtccggcgct ctacnnnacg acnaancnnt ttcnnnncna cctcnnnntg nnggngctcg acnatctgcn nncnntngan ganntnnnnc gcgcatntng angcgagccg ctcgngnnna ggcnatcnat ttnnnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNTQEAKIV LETALICAQE PLKLGDLRKL FADGVSADTV RTLLEDLKQE WTGRGVELVA LASGWRFQSK PAMRTYLDRL HPEKPPKYSR AVLETLAIIA YRQPVTRGDI EEIRGVTVNT QVVKQLEDRG WIEVIGHRDV PGRPALYATT KQFLDDLGLK ALDDLPALEE PAAHLEASLL AQQAIDFPDD ARGDGEAPAV APETDAAAAQ AGEVAEPASD SVVLRNEDTA RADEPRQADA APTRADEPRQ EGATDGLAAR IGDPADNDAG RADVDVDAAP ALGEADAPRG GDASGHAARE IDSREMTEPA DAGGEAAGTT ASHTHAVAPY DHDAQSAADA VAAPHAGDVA ADGERAESPS GTNENANYVA QPDAEPADAA HAATREVAAD PAAGRATKED HGAIGGIPHD AEPVRAHAEE ALDDTSDSLA DAVRSASEAA PEHERQDDED APAKRRA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatacccaa gaggcgaaga tcgtcctcga gactgccttg atctgcgcgc aggaaccgct gaagctcggc gatttgcgca agctctttgc cgacggcgtg tcggcggata cagtccgaac actgctcgaa gatctgaaac aggaatggac cggacggggg gtcgaactcg tcgcgctcgc gtcgggctgg cgcttccaga gcaagccggc gatgcgcacg tatctcgatc gcctgcatcc cgagaagccg ccgaagtatt cgcgcgccgt gctggagacg ctcgcgatca tcgcgtaccg gcagccggtc acgcgcggcg acatcgagga gatccgcggc gtcacggtga acacgcaggt cgtcaagcag ctcgaggacc gcggctggat cgaagtgatc ggtcatcgcg acgtgccggg ccgtccggcg ctctacgcga cgacaaaaca gtttctcgac gacctcggcc tgaaggcgct cgacgatctg cccgcgctcg aggagccggc cgcgcatctg gaggcgagcc tgctcgcgca gcaggcgatc gatttccccg atgacgcgcg cggcgacggc gaagcgcctg ccgtcgcgcc cgagacggac gccgccgccg cgcaggcggg cgaggttgcc gagccggcga gcgattcggt ggttttgcgc aacgaagata cggcgcgcgc cgacgagccg cggcaggcgg acgcggcgcc gacgcgtgcc gacgagccgc ggcaggaagg ggcgacggac ggcttggcgg cacggatcgg cgatccagcc gataatgacg ccgggcgagc cgatgtggac gtcgacgcgg cgccggctct cggcgaagcc gatgcgccgc gcggcggcga tgcttcaggg catgcggctc gcgagatcga ctcgcgcgag atgacggagc cggcagacgc cggcggcgag gcggccggca cgaccgcatc gcatacgcat gcggtcgcgc cgtacgacca tgatgcgcag agcgcagcgg acgccgtcgc ggcgccgcac gccggcgatg tagccgctga tggcgagcgc gccgaatcgc cctcggggac gaacgaaaac gcaaactacg tcgcgcagcc ggacgcggag ccggccgacg cggcacacgc agcaacacgc gaggtggccg ccgacccggc ggcgggccga gcgacgaagg aagaccacgg cgcgatcggc ggcatcccgc acgatgccga gccggtccgg gcgcacgcgg aggaggcgtt ggacgacacg tccgacagtc tcgccgacgc cgtgcgcagt gcaagcgagg cggcccccga gcacgagcgg caggacgacg aggacgcgcc tgcgaagcgt cgcgcctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BupsA.17551.a.A1.PS01141

PURIFICATION DATe:	7/1/2011
CONCENTRATION:	91.96mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	100µl
PERCENT IDENTITY:	74
PERCENT COVERAGE:	39

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATACCCAA GAGGCGAAGA TCGTCCTCGA GACTGCCTTG ATCTGCGCGC AGGAACCGCT GAAGCTCGGC GATTTGCGCA AGCTCTTTGC CGACGGCGTG TCGGCGGATA CAGTCCGAAC ACTGCTCGAA GATCTGAAAC AGGAATGGAC CGGACGGGGG GTCGAACTCG TCGCGCTCGC GTCGGGCTGG CGCTTCCAGA GCAAGCCGGC GATGCGCACG TATCTCGATC GCCTGCATCC CGAGAAGCCG CCGAAGTATT CGCGCGCCGT GCTGGAGACG CTCGCGATCA TCGCGTACCG GCAGCCGGTC ACGCGCGGCG ACATCGAGGA GATCCGCGGC GTCACGGTGA ACACGCAGGT CGTCAAGCAG CTCGAGGACC GCGGCTGGAT CGAAGTGATC GGTCATCGCG ACGTGCCGGG CCGTCCGGCG CTCTACGCGA CGACAAAACA GTTTCTCGAC GACCTCGGCC TGAAGGCGCT CGACGATCTG CCCGCGCTCG AGGAGCCGGC CGCGCATCTG GAGGCGAGCC TGCTCGCGCA GCAGGCGATC GATTTCCCCG ATGACGCGCG CGGCGACGGC GAAGCGCCTG CCGTCGCGCC CGAGACGGAC GCCGCCGCCG CGCAGGCGGG CGAGGTTGCC GAGCCGGCGA GCGATTCGGT GGTTTTGCGC AACGAAGATA CGGCGCGCGC CGACGAGCCG CGGCAGGCGG ACGCGGCGCC GACGCGTGCC GACGAGCCGC GGCAGGAAGG GGCGACGGAC GGCTTGGCGG CACGGATCGG CGATCCAGCC GATAATGACG CCGGGCGAGC CGATGTGGAC GTCGACGCGG CGCCGGCTCT CGGCGAAGCC GATGCGCCGC GCGGCGGCGA TGCTTCAGGG CATGCGGCTC GCGAGATCGA CTCGCGCGAG ATGACGGAGC CGGCAGACGC CGGCGGCGAG GCGGCCGGCA CGACCGCATC GCATACGCAT GCGGTCGCGC CGTACGACCA TGATGCGCAG AGCGCAGCGG ACGCCGTCGC GGCGCCGCAC GCCGGCGATG TAGCCGCTGA TGGCGAGCGC GCCGAATCGC CCTCGGGGAC GAACGAAAAC GCAAACTACG TCGCGCAGCC GGACGCGGAG CCGGCCGACG CGGCACACGC AGCAACACGC GAGGTGGCCG CCGACCCGGC GGCGGGCCGA GCGACGAAGG AAGACCACGG CGCGATCGGC GGCATCCCGC ACGATGCCGA GCCGGTCCGG GCGCACGCGG AGGAGGCGTT GGACGACACG TCCGACAGTC TCGCCGACGC CGTGCGCAGT GCAAGCGAGG CGGCCCCCGA GCACGAGCGG CAGGACGACG AGGACGCGCC TGCGAAGCGT CGCGCCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA