BupsA.17546.a

Phosphotransferase enzyme family domain protein

CENTER ID: BupsA.17546.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17546.a.A1.GE31334 Full length( BupsA.17546.a ) 1 401
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.879
RefSeq: YP_332283.1
UniProt: Q3JVX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRPRNRTVT AASRIPGETG KRTAWRAAGP RRRELTRDAS KKSWGIIWQD VPPPRLSMTQ PPADPRLARL TAWLATFADR YALELASLAP ASADASFRRY FRVASAAAPG GAAIAVDAPP PEKCREFAQI AQLLDAAGVH VPKVLEHDFD AGFMLVTDLG TRPYISALDP AAPAAARPLM RDALDALIRW QRASREGVLP PFDEAFLRRE MALMPEWYVG RHLGKALSDD ARGVLDRTFA LLAASAKAQP QGYMLRDFMP RNLMIAEPNP GVLDFQDAVY GPLTYDVVSL LRDAFISWDE EFELDCFAYY WEQAKKAGLP VEPDFGEFYR QLEWMGLQRH IKVLGLFARI NYRDHKPHYL ADLPRFLGYA RRVALRYRPL APFAKLLDDL EGKTADVGYT F
NT Sequence
ATGCCGCGCC CGCGCAATCG AACCGTGACT GCGGCTTCGC GCATACCGGG GGAAACGGGC AAACGGACGG CATGGCGGGC TGCGGGCCCG CGCCGGCGGG AATTGACACG GGACGCGTCA AAAAAGTCGT GGGGTATTAT ATGGCAAGAC GTTCCCCCTC CCCGACTTTC AATGACGCAG CCCCCAGCCG ATCCCCGTCT CGCCCGCCTC ACCGCCTGGC TCGCCACTTT CGCCGATCGC TACGCGCTCG AGCTCGCCTC GCTCGCCCCG GCGTCCGCCG ACGCGAGCTT TCGCCGCTAT TTCCGCGTCG CGAGCGCGGC CGCGCCGGGC GGCGCGGCGA TCGCCGTCGA CGCGCCGCCG CCGGAGAAAT GCCGCGAGTT CGCGCAGATC GCGCAACTGC TCGACGCGGC GGGCGTCCAC GTGCCGAAGG TGCTCGAGCA CGACTTCGAC GCGGGCTTCA TGCTCGTCAC CGATCTCGGC ACGCGCCCGT ACATCTCGGC GCTCGATCCC GCCGCGCCCG CCGCCGCGCG GCCGCTGATG CGCGACGCGC TCGACGCGCT GATCCGCTGG CAGCGCGCGT CGCGCGAGGG CGTGCTGCCG CCGTTCGACG AAGCGTTCCT GCGCCGCGAG ATGGCGCTGA TGCCCGAATG GTACGTCGGC CGGCATCTCG GCAAGGCGCT CTCGGACGAC GCGCGCGGCG TGCTCGATCG CACGTTCGCG CTGTTGGCCG CGAGCGCGAA GGCGCAGCCG CAGGGGTACA TGCTGCGCGA CTTCATGCCC CGCAACCTGA TGATCGCGGA GCCGAACCCG GGCGTGCTCG ACTTCCAGGA CGCCGTGTAC GGACCGCTCA CGTACGACGT CGTGTCGCTC CTGCGCGACG CGTTCATCAG TTGGGACGAG GAATTCGAGC TCGACTGCTT CGCGTACTAC TGGGAACAGG CGAAGAAGGC GGGGCTGCCC GTCGAGCCGG ATTTCGGCGA GTTCTACCGG CAGCTCGAAT GGATGGGCCT GCAGCGCCAC ATCAAGGTGC TCGGGCTGTT CGCTCGAATC AATTACCGCG ATCACAAGCC GCACTATCTC GCCGATCTGC CGCGCTTTCT AGGCTACGCG CGCCGCGTCG CGCTGCGCTA CCGGCCGCTC GCGCCGTTCG CGAAGCTCCT CGACGATCTC GAAGGCAAGA CGGCCGACGT CGGCTATACG TTC
Details for BupsA.17546.a.A1.GE31334
HARVESTED ON: 1/26/2011
SEQUENCED ON: 1/31/2011
EXPECTED MW: 47kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 71
PERCENT COVERAGE: 52
Validated AA Sequence
MFXXLXXXXX XXXXXXXXSS XXGPGSMPRP RNRTVTAASR IPGETGKRTA WRAAGPRRRE LTRDASKKSW GIIWQDVPPP RLSMTQPPAD PRLARLTAWL ATFADRYALE LASLAPASAD ASFRRYFRVA SAAAPGGAAI AVDAPPPEKC REFAQIAQLL DAAGVHVPXV LXTTSTRASC SXXXSXRART SRXXXXXXXX PRXXDARXXX XXXXXXXRXX X
Validated NT Sequence
gnnnngccat gtttnnnnnn ctcnnnnnnn nnnnnannna annannannn gnnnncannn nnagctcana ccnnggtcct ggttcgatgc cgcgcccgcg caatcgaacc gtgactgcgg cttcgcgcat accgggggaa acgggcaaac ggacggcatg gcgggctgcg ggcccgcgcc ggcgggaatt gacacgggac gcgtcaaaaa agtcgtgggg tattatatgg caagacgttc cccctccccg actttcaatg acgcagcccc cagccgatcc ccgtctcgcc cgcctcaccg cctggctcgc cactttcgcc gatcgctacg cgctcgagct cgcctcgctc gccccggcgt ccgccgacgc gagctttcgc cgctatttcc gcgtcgcgag cgcggccgcg ccgggcggcg cggcgatcgc cgtcgacgcg ccgccgccgg agaaatgccg cgagttcgcg cagatcgcgc aactgctcga cgcggcgggc gtccacgtgc cgaangtgct cancacgact tcgacgcggg cttcntgctc gtnnnnnanc tcggnacgcg cccgtacatc tcggngcnnn annnngncgc ncnngncgcc gcgcgnnnnc gatgcgcgac nnnntcnann nnctgntncn ntnnnnnngc gcgntnnnnn nng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPRPRNRTV TAASRIPGET GKRTAWRAAG PRRRELTRDA SKKSWGIIWQ DVPPPRLSMT QPPADPRLAR LTAWLATFAD RYALELASLA PASADASFRR YFRVASAAAP GGAAIAVDAP PPEKCREFAQ IAQLLDAAGV HVPKVLEHDF DAGFMLVTDL GTRPYISALD PAAPAAARPL MRDALDALIR WQRASREGVL PPFDEAFLRR EMALMPEWYV GRHLGKALSD DARGVLDRTF ALLAASAKAQ PQGYMLRDFM PRNLMIAEPN PGVLDFQDAV YGPLTYDVVS LLRDAFISWD EEFELDCFAY YWEQAKKAGL PVEPDFGEFY RQLEWMGLQR HIKVLGLFAR INYRDHKPHY LADLPRFLGY ARRVALRYRP LAPFAKLLDD LEGKTADVGY TF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgcgcccg cgcaatcgaa ccgtgactgc ggcttcgcgc ataccggggg aaacgggcaa acggacggca tggcgggctg cgggcccgcg ccggcgggaa ttgacacggg acgcgtcaaa aaagtcgtgg ggtattatat ggcaagacgt tccccctccc cgactttcaa tgacgcagcc cccagccgat ccccgtctcg cccgcctcac cgcctggctc gccactttcg ccgatcgcta cgcgctcgag ctcgcctcgc tcgccccggc gtccgccgac gcgagctttc gccgctattt ccgcgtcgcg agcgcggccg cgccgggcgg cgcggcgatc gccgtcgacg cgccgccgcc ggagaaatgc cgcgagttcg cgcagatcgc gcaactgctc gacgcggcgg gcgtccacgt gccgaaggtg ctcgagcacg acttcgacgc gggcttcatg ctcgtcaccg atctcggcac gcgcccgtac atctcggcgc tcgatcccgc cgcgcccgcc gccgcgcggc cgctgatgcg cgacgcgctc gacgcgctga tccgctggca gcgcgcgtcg cgcgagggcg tgctgccgcc gttcgacgaa gcgttcctgc gccgcgagat ggcgctgatg cccgaatggt acgtcggccg gcatctcggc aaggcgctct cggacgacgc gcgcggcgtg ctcgatcgca cgttcgcgct gttggccgcg agcgcgaagg cgcagccgca ggggtacatg ctgcgcgact tcatgccccg caacctgatg atcgcggagc cgaacccggg cgtgctcgac ttccaggacg ccgtgtacgg accgctcacg tacgacgtcg tgtcgctcct gcgcgacgcg ttcatcagtt gggacgagga attcgagctc gactgcttcg cgtactactg ggaacaggcg aagaaggcgg ggctgcccgt cgagccggat ttcggcgagt tctaccggca gctcgaatgg atgggcctgc agcgccacat caaggtgctc gggctgttcg ctcgaatcaa ttaccgcgat cacaagccgc actatctcgc cgatctgccg cgctttctag gctacgcgcg ccgcgtcgcg ctgcgctacc ggccgctcgc gccgttcgcg aagctcctcg acgatctcga aggcaagacg gccgacgtcg gctatacgtt ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa