BupsA.17538.a

L-2-Amino-thiazoline-4-carboxylic acid hydrolase

CENTER ID: BupsA.17538.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17538.a.A1.GE31332 Full length( BupsA.17538.a ) 1 408
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.6662
RefSeq: YP_337605.1
UniProt: Q3JFP8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSYVLMRAMH AIRGAAERAD STDAARLPAV LHTLKRQGVT GAIAFGGEGN LRDPAFTIYR AGGRTWAVVG VLGGVGGVGG VGASGNAAAC VAAGVSGLSG GKAARLARFR ESRERIERRI EMATSPGGRP MTTIPIHPAA GGAAHDEPLG ILARRRIEAE IIKPIYEIMK REFGLERAQA VIAEAVRGAA LDAGRAFAAK EPGGTSIASF VALQVLWEKD DALDVDVHRA DDAHYDYDVR RCAYAQMYRE MGLAEIGHLL SCARDSVFIE GYDARIALTR TRTLMQGGTH CDFRYRLAQP PRDGAADATH AAPGGLAGGM REARVRENEP AGQPEPAPGD RAGPSPESPP RSRAESRTET GTEPGTASQA SGDAPGIRRA SSPTFDRRAV AASPAGEHTR PEAGDAAR
NT Sequence
GTGTCATACG TGCTGATGCG CGCCATGCAC GCCATACGCG GTGCCGCGGA GCGTGCGGAT TCGACCGACG CCGCGCGCCT GCCGGCGGTG CTCCATACGT TGAAGCGGCA AGGCGTGACG GGCGCGATCG CGTTCGGCGG CGAAGGCAAT CTGCGGGACC CGGCGTTCAC GATCTATCGG GCGGGCGGCC GCACATGGGC GGTGGTCGGC GTGCTCGGCG GTGTCGGCGG TGTCGGCGGT GTCGGCGCGA GCGGCAACGC GGCGGCCTGC GTCGCGGCGG GCGTGTCCGG CTTGTCCGGC GGTAAGGCCG CCCGGCTTGC CCGGTTTCGC GAAAGTCGCG AACGCATCGA AAGACGCATC GAAATGGCAA CCAGTCCAGG AGGCAGACCG ATGACGACTA TTCCGATCCA CCCGGCCGCG GGCGGCGCGG CGCACGACGA GCCGCTCGGC ATCCTCGCGC GGCGGCGCAT CGAGGCCGAG ATCATCAAGC CGATCTACGA GATCATGAAG CGCGAGTTCG GCCTCGAGCG CGCGCAGGCG GTGATCGCGG AGGCCGTGCG CGGCGCGGCG CTCGACGCGG GGCGCGCGTT TGCCGCGAAG GAGCCGGGCG GCACGAGCAT CGCGTCGTTC GTCGCGCTGC AGGTGCTATG GGAGAAGGAC GATGCGCTCG ACGTCGACGT GCATCGCGCG GACGACGCGC ATTACGACTA CGACGTGCGC CGCTGCGCGT ACGCGCAGAT GTATCGCGAG ATGGGGCTCG CCGAGATCGG GCATCTGTTG AGCTGCGCGC GCGACAGCGT GTTCATCGAG GGCTACGATG CGCGCATCGC GCTCACGCGC ACGCGCACGC TGATGCAGGG CGGCACGCAT TGCGACTTTC GTTACCGGCT GGCGCAGCCG CCGCGAGACG GCGCGGCGGA CGCAACGCAT GCGGCGCCGG GCGGGCTCGC CGGCGGCATG CGCGAGGCGC GGGTGCGGGA GAACGAGCCG GCCGGACAGC CGGAACCCGC GCCGGGGGAT CGGGCGGGGC CTTCGCCGGA ATCGCCGCCG CGATCGCGGG CGGAATCTCG AACGGAAACC GGAACGGAAC CCGGGACGGC ATCCCAGGCG AGCGGCGATG CGCCGGGCAT TCGTCGAGCG TCTTCCCCCA CGTTCGATCG GCGCGCCGTC GCAGCGTCGC CCGCCGGCGA GCACACACGC CCGGAGGCGG GCGATGCGGC GCGA
Details for BupsA.17538.a.A1.GE31332
HARVESTED ON: 1/26/2011
SEQUENCED ON: 1/31/2011
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 30
Validated AA Sequence
MVSYVLMRAM HAIRGAAERA DSTDAARLPA XLHTLXRQGV TGAIAFGGEG NLRDPAFTIY RAGGRTWAXV GVLGGVXGVX GVGASGXAAA CVAAGVXGLS GGKAXXXXRF RESRXRXXRR XQMAXXXAXR
Validated NT Sequence
ggnnnagcca tgtttacnnn nnntcctntn nnnnannnnn annnaannac nnnnnnnnnn cannnnnnnn tcanaccann ggtcctggtt cgatggtgtc atacgtgctg atgcgcgcca tgcacgccat acgcggtgcc gcggagcgtg cggattcaac cgacgccgcg cgcctgccgg cggngctcca tacgttgaan cggcaaggcg tgacgggcgc gatcgcgttc ggcggcgaag gcaatctgcg ggacccggcg ttcacgatct atcgggcggg cggccgcaca tgggcggngg tcggngtgct cggcggtgtc ngcggtgtcn gcggtgtcgg cgcnagcggn nacgcggcgg cctgcgtcgc ggcgggngtg tncggcttgt ccggnggnaa ggccgnncng nntgnccggt ttcgcgaaag tcgnnaacgc atnnaaagac gcanccaaat ggcnncnann tangcanacc gatgangant attnnnatcc nncnngnnnn nnnnngngcg cnaacnanca gnctntcgnn nnnntcncnn ggntnnnnan ntnggnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSYVLMRA MHAIRGAAER ADSTDAARLP AVLHTLKRQG VTGAIAFGGE GNLRDPAFTI YRAGGRTWAV VGVLGGVGGV GGVGASGNAA ACVAAGVSGL SGGKAARLAR FRESRERIER RIEMATSPGG RPMTTIPIHP AAGGAAHDEP LGILARRRIE AEIIKPIYEI MKREFGLERA QAVIAEAVRG AALDAGRAFA AKEPGGTSIA SFVALQVLWE KDDALDVDVH RADDAHYDYD VRRCAYAQMY REMGLAEIGH LLSCARDSVF IEGYDARIAL TRTRTLMQGG THCDFRYRLA QPPRDGAADA THAAPGGLAG GMREARVREN EPAGQPEPAP GDRAGPSPES PPRSRAESRT ETGTEPGTAS QASGDAPGIR RASSPTFDRR AVAASPAGEH TRPEAGDAAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcatac gtgctgatgc gcgccatgca cgccatacgc ggtgccgcgg agcgtgcgga ttcgaccgac gccgcgcgcc tgccggcggt gctccatacg ttgaagcggc aaggcgtgac gggcgcgatc gcgttcggcg gcgaaggcaa tctgcgggac ccggcgttca cgatctatcg ggcgggcggc cgcacatggg cggtggtcgg cgtgctcggc ggtgtcggcg gtgtcggcgg tgtcggcgcg agcggcaacg cggcggcctg cgtcgcggcg ggcgtgtccg gcttgtccgg cggtaaggcc gcccggcttg cccggtttcg cgaaagtcgc gaacgcatcg aaagacgcat cgaaatggca accagtccag gaggcagacc gatgacgact attccgatcc acccggccgc gggcggcgcg gcgcacgacg agccgctcgg catcctcgcg cggcggcgca tcgaggccga gatcatcaag ccgatctacg agatcatgaa gcgcgagttc ggcctcgagc gcgcgcaggc ggtgatcgcg gaggccgtgc gcggcgcggc gctcgacgcg gggcgcgcgt ttgccgcgaa ggagccgggc ggcacgagca tcgcgtcgtt cgtcgcgctg caggtgctat gggagaagga cgatgcgctc gacgtcgacg tgcatcgcgc ggacgacgcg cattacgact acgacgtgcg ccgctgcgcg tacgcgcaga tgtatcgcga gatggggctc gccgagatcg ggcatctgtt gagctgcgcg cgcgacagcg tgttcatcga gggctacgat gcgcgcatcg cgctcacgcg cacgcgcacg ctgatgcagg gcggcacgca ttgcgacttt cgttaccggc tggcgcagcc gccgcgagac ggcgcggcgg acgcaacgca tgcggcgccg ggcgggctcg ccggcggcat gcgcgaggcg cgggtgcggg agaacgagcc ggccggacag ccggaacccg cgccggggga tcgggcgggg ccttcgccgg aatcgccgcc gcgatcgcgg gcggaatctc gaacggaaac cggaacggaa cccgggacgg catcccaggc gagcggcgat gcgccgggca ttcgtcgagc gtcttccccc acgttcgatc ggcgcgccgt cgcagcgtcg cccgccggcg agcacacacg cccggaggcg ggcgatgcgg cgcgataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa