BupsA.17533.a

CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehy drase reductase

CENTER ID: BupsA.17533.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17533.a.A1.GE31329 Full length( BupsA.17533.a ) 1 343
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17533.a.A1.PS01031 Full length( BupsA.17533.a ) 1 343
BupsA.17533.a.A1.PS01032 Full length( BupsA.17533.a ) 1 343

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.1161
RefSeq: YP_332563.1
UniProt: Q3JV40

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAFNVTLKQS GRQFQVEADE TVLAAALRQN VHLPYGCKNG ACGSCKGTIV QGQFEQGPHS ASALSNDERT RGLALLCCSK PQSDLEVDVR EIAGVDGVQV KKLPCRVAAL ERRADDVIVL KLQLPANERL QYLAGQYIEF ILKDGARRSY SMATAPHEEG PIELHIRHMP GGKFTDHVFG PMKERDILRF EGPLGTFFLR EDSDKPIVLL ASGTGFAPIK AIIEHAHHVK LERPMTLYWG ARRKKDLYLL DAAEQWAKEI PNFKFVPVLS EPDASDAWTG RTGFVHRAVI EDLPDLSGHQ VYACGAPVMV ESAQRDFTQH HRLPADEFYA DSFTSAADLA HTV
NT Sequence
ATGGCTTTTA ACGTAACCCT CAAGCAAAGC GGCCGGCAGT TCCAGGTCGA GGCCGACGAA ACCGTGCTCG CGGCGGCGCT GCGTCAGAAC GTCCATCTGC CCTACGGCTG CAAGAACGGC GCGTGCGGCT CGTGCAAGGG CACGATCGTG CAAGGCCAGT TCGAGCAGGG ACCGCATTCC GCGTCGGCGC TGTCGAACGA CGAGCGCACG CGCGGCCTCG CGCTGCTGTG CTGCTCGAAG CCGCAGAGCG ATCTCGAGGT CGACGTGCGC GAGATCGCGG GCGTCGACGG CGTTCAGGTG AAGAAGCTGC CGTGTCGCGT CGCGGCGCTC GAGCGCCGCG CGGACGACGT GATCGTGCTG AAGCTGCAGT TGCCCGCGAA CGAGCGCCTG CAGTATCTGG CCGGCCAGTA CATCGAGTTC ATCCTGAAGG ACGGCGCGCG CCGCAGCTAC TCGATGGCGA CCGCGCCGCA CGAGGAAGGT CCGATCGAGC TGCACATCCG CCACATGCCG GGCGGCAAGT TCACCGACCA CGTGTTCGGC CCGATGAAGG AGCGCGACAT CCTGCGCTTC GAAGGCCCGC TCGGCACGTT CTTCCTGCGC GAGGATTCGG ACAAGCCGAT CGTGCTGCTC GCCTCCGGCA CGGGCTTCGC GCCGATCAAG GCGATCATCG AGCACGCGCA CCACGTGAAG CTCGAGCGCC CGATGACGCT CTACTGGGGC GCGCGCCGCA AGAAAGATCT GTATCTGCTC GACGCCGCCG AGCAATGGGC GAAGGAGATC CCGAACTTCA AGTTCGTGCC GGTGCTTTCC GAGCCCGACG CGAGCGACGC GTGGACAGGC CGCACGGGCT TCGTGCACCG CGCGGTGATC GAGGATCTGC CCGACCTGTC CGGCCATCAG GTGTACGCGT GCGGCGCGCC GGTGATGGTC GAATCCGCGC AGCGCGACTT CACGCAGCAC CACCGCCTGC CCGCCGACGA GTTCTACGCG GATTCGTTCA CGAGCGCCGC GGACCTCGCG CATACGGTC
Details for BupsA.17533.a.A1.GE31329
HARVESTED ON: 1/26/2011
SEQUENCED ON: 1/31/2011
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 18
PERCENT COVERAGE: 96
Validated AA Sequence
MREVRGARER IRVELVGGQA VVLREVALRG FDHHRRAARV HLMAGQVGQI LDHRAVHEAR AACPRVARVG LGKHRHELEV RDLLRPLLGG VEQIQIFLAA RAPVERHRAL ELHVVRVLDD RLDRREARAG GEQHDRLVRI LAQEERAERA FEAQDVALLH RAEHVVGELA ARHVADVQLD RTFLVRRGRH RVAAARAVLQ DELDVLAGQI LQALVRGQLQ LQHDHVVRAA LERRDATRQL LHLNAVDARD LAHVDLEIAL RLRAAQQREA ARALVVRQRR RGMRSLLELA LHDRALARAA RAVLAAVGQM DVLTQRRREH GFVGLDLELP AALLEGYVKS HRTRTLGLSF QGT
Validated NT Sequence
ttattagacc gtatgcgcga ggtccgcggc gctcgtgaac gaatccgcgt agaactcgtc ggcgggcagg cggtggtgct gcgtgaagtc gcgctgcgcg gattcgacca tcaccggcgc gccgcacgcg tacacctgat ggccggacag gtcgggcaga tcctcgatca ccgcgcggtg cacgaagccc gtgcggcctg tccacgcgtc gctcgcgtcg ggctcggaaa gcaccggcac gaacttgaag ttcgggatct ccttcgccca ttgctcggcg gcgtcgagca gatacagatc tttcttgcgg cgcgcgcccc agtagagcgt catcgggcgc tcgagcttca cgtggtgcgc gtgctcgatg atcgccttga tcggcgcgaa gcccgtgccg gaggcgagca gcacgatcgg cttgtccgaa tcctcgcgca ggaagaacgt gccgagcggg ccttcgaagc gcaggatgtc gcgctccttc atcgggccga acacgtggtc ggtgaacttg ccgcccggca tgtggcggat gtgcagctcg atcggacctt cctcgtgcgg cgcggtcgcc atcgagtagc tgcggcgcgc gccgtccttc aggatgaact cgatgtactg gccggccaga tactgcaggc gctcgttcgc gggcaactgc agcttcagca cgatcacgtc gtccgcgcgg cgctcgagcg ccgcgacgcg acacggcagc ttcttcacct gaacgccgtc gacgcccgcg atctcgcgca cgtcgacctc gagatcgctc tgcggcttcg agcagcacag cagcgcgagg ccgcgcgtgc gctcgtcgtt cgacagcgcc gacgcggaat gcggtccctg ctcgaactgg ccttgcacga tcgtgccctt gcacgagccg cacgcgccgt tcttgcagcc gtagggcaga tggacgttct gacgcagcgc cgccgcgagc acggtttcgt cggcctcgac ctggaactgc cggccgcttt gcttgagggt tacgttaaaa gccatcgaac caggaccctg ggtctgagct tccagggtac cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFNVTLKQ SGRQFQVEAD ETVLAAALRQ NVHLPYGCKN GACGSCKGTI VQGQFEQGPH SASALSNDER TRGLALLCCS KPQSDLEVDV REIAGVDGVQ VKKLPCRVAA LERRADDVIV LKLQLPANER LQYLAGQYIE FILKDGARRS YSMATAPHEE GPIELHIRHM PGGKFTDHVF GPMKERDILR FEGPLGTFFL REDSDKPIVL LASGTGFAPI KAIIEHAHHV KLERPMTLYW GARRKKDLYL LDAAEQWAKE IPNFKFVPVL SEPDASDAWT GRTGFVHRAV IEDLPDLSGH QVYACGAPVM VESAQRDFTQ HHRLPADEFY ADSFTSAADL AHTV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcttttaac gtaaccctca agcaaagcgg ccggcagttc caggtcgagg ccgacgaaac cgtgctcgcg gcggcgctgc gtcagaacgt ccatctgccc tacggctgca agaacggcgc gtgcggctcg tgcaagggca cgatcgtgca aggccagttc gagcagggac cgcattccgc gtcggcgctg tcgaacgacg agcgcacgcg cggcctcgcg ctgctgtgct gctcgaagcc gcagagcgat ctcgaggtcg acgtgcgcga gatcgcgggc gtcgacggcg ttcaggtgaa gaagctgccg tgtcgcgtcg cggcgctcga gcgccgcgcg gacgacgtga tcgtgctgaa gctgcagttg cccgcgaacg agcgcctgca gtatctggcc ggccagtaca tcgagttcat cctgaaggac ggcgcgcgcc gcagctactc gatggcgacc gcgccgcacg aggaaggtcc gatcgagctg cacatccgcc acatgccggg cggcaagttc accgaccacg tgttcggccc gatgaaggag cgcgacatcc tgcgcttcga aggcccgctc ggcacgttct tcctgcgcga ggattcggac aagccgatcg tgctgctcgc ctccggcacg ggcttcgcgc cgatcaaggc gatcatcgag cacgcgcacc acgtgaagct cgagcgcccg atgacgctct actggggcgc gcgccgcaag aaagatctgt atctgctcga cgccgccgag caatgggcga aggagatccc gaacttcaag ttcgtgccgg tgctttccga gcccgacgcg agcgacgcgt ggacaggccg cacgggcttc gtgcaccgcg cggtgatcga ggatctgccc gacctgtccg gccatcaggt gtacgcgtgc ggcgcgccgg tgatggtcga atccgcgcag cgcgacttca cgcagcacca ccgcctgccc gccgacgagt tctacgcgga ttcgttcacg agcgccgcgg acctcgcgca tacggtctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BupsA.17533.a.A1.PS01031
PURIFICATION DATe: 4/5/2011
CONCENTRATION: 40.26mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 50µl
PERCENT IDENTITY: 18
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MREVRGARER IRVELVGGQA VVLREVALRG FDHHRRAARV HLMAGQVGQI LDHRAVHEAR AACPRVARVG LGKHRHELEV RDLLRPLLGG VEQIQIFLAA RAPVERHRAL ELHVVRVLDD RLDRREARAG GEQHDRLVRI LAQEERAERA FEAQDVALLH RAEHVVGELA ARHVADVQLD RTFLVRRGRH RVAAARAVLQ DELDVLAGQI LQALVRGQLQ LQHDHVVRAA LERRDATRQL LHLNAVDARD LAHVDLEIAL RLRAAQQREA ARALVVRQRR RGMRSLLELA LHDRALARAA RAVLAAVGQM DVLTQRRREH GFVGLDLELP AALLEGYVKS HRTRTLGLSF QGT
Validated NT Sequence
ttattagacc gtatgcgcga ggtccgcggc gctcgtgaac gaatccgcgt agaactcgtc ggcgggcagg cggtggtgct gcgtgaagtc gcgctgcgcg gattcgacca tcaccggcgc gccgcacgcg tacacctgat ggccggacag gtcgggcaga tcctcgatca ccgcgcggtg cacgaagccc gtgcggcctg tccacgcgtc gctcgcgtcg ggctcggaaa gcaccggcac gaacttgaag ttcgggatct ccttcgccca ttgctcggcg gcgtcgagca gatacagatc tttcttgcgg cgcgcgcccc agtagagcgt catcgggcgc tcgagcttca cgtggtgcgc gtgctcgatg atcgccttga tcggcgcgaa gcccgtgccg gaggcgagca gcacgatcgg cttgtccgaa tcctcgcgca ggaagaacgt gccgagcggg ccttcgaagc gcaggatgtc gcgctccttc atcgggccga acacgtggtc ggtgaacttg ccgcccggca tgtggcggat gtgcagctcg atcggacctt cctcgtgcgg cgcggtcgcc atcgagtagc tgcggcgcgc gccgtccttc aggatgaact cgatgtactg gccggccaga tactgcaggc gctcgttcgc gggcaactgc agcttcagca cgatcacgtc gtccgcgcgg cgctcgagcg ccgcgacgcg acacggcagc ttcttcacct gaacgccgtc gacgcccgcg atctcgcgca cgtcgacctc gagatcgctc tgcggcttcg agcagcacag cagcgcgagg ccgcgcgtgc gctcgtcgtt cgacagcgcc gacgcggaat gcggtccctg ctcgaactgg ccttgcacga tcgtgccctt gcacgagccg cacgcgccgt tcttgcagcc gtagggcaga tggacgttct gacgcagcgc cgccgcgagc acggtttcgt cggcctcgac ctggaactgc cggccgcttt gcttgagggt tacgttaaaa gccatcgaac caggaccctg ggtctgagct tccagggtac cc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFNVTLKQ SGRQFQVEAD ETVLAAALRQ NVHLPYGCKN GACGSCKGTI VQGQFEQGPH SASALSNDER TRGLALLCCS KPQSDLEVDV REIAGVDGVQ VKKLPCRVAA LERRADDVIV LKLQLPANER LQYLAGQYIE FILKDGARRS YSMATAPHEE GPIELHIRHM PGGKFTDHVF GPMKERDILR FEGPLGTFFL REDSDKPIVL LASGTGFAPI KAIIEHAHHV KLERPMTLYW GARRKKDLYL LDAAEQWAKE IPNFKFVPVL SEPDASDAWT GRTGFVHRAV IEDLPDLSGH QVYACGAPVM VESAQRDFTQ HHRLPADEFY ADSFTSAADL AHTV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTTTTAAC GTAACCCTCA AGCAAAGCGG CCGGCAGTTC CAGGTCGAGG CCGACGAAAC CGTGCTCGCG GCGGCGCTGC GTCAGAACGT CCATCTGCCC TACGGCTGCA AGAACGGCGC GTGCGGCTCG TGCAAGGGCA CGATCGTGCA AGGCCAGTTC GAGCAGGGAC CGCATTCCGC GTCGGCGCTG TCGAACGACG AGCGCACGCG CGGCCTCGCG CTGCTGTGCT GCTCGAAGCC GCAGAGCGAT CTCGAGGTCG ACGTGCGCGA GATCGCGGGC GTCGACGGCG TTCAGGTGAA GAAGCTGCCG TGTCGCGTCG CGGCGCTCGA GCGCCGCGCG GACGACGTGA TCGTGCTGAA GCTGCAGTTG CCCGCGAACG AGCGCCTGCA GTATCTGGCC GGCCAGTACA TCGAGTTCAT CCTGAAGGAC GGCGCGCGCC GCAGCTACTC GATGGCGACC GCGCCGCACG AGGAAGGTCC GATCGAGCTG CACATCCGCC ACATGCCGGG CGGCAAGTTC ACCGACCACG TGTTCGGCCC GATGAAGGAG CGCGACATCC TGCGCTTCGA AGGCCCGCTC GGCACGTTCT TCCTGCGCGA GGATTCGGAC AAGCCGATCG TGCTGCTCGC CTCCGGCACG GGCTTCGCGC CGATCAAGGC GATCATCGAG CACGCGCACC ACGTGAAGCT CGAGCGCCCG ATGACGCTCT ACTGGGGCGC GCGCCGCAAG AAAGATCTGT ATCTGCTCGA CGCCGCCGAG CAATGGGCGA AGGAGATCCC GAACTTCAAG TTCGTGCCGG TGCTTTCCGA GCCCGACGCG AGCGACGCGT GGACAGGCCG CACGGGCTTC GTGCACCGCG CGGTGATCGA GGATCTGCCC GACCTGTCCG GCCATCAGGT GTACGCGTGC GGCGCGCCGG TGATGGTCGA ATCCGCGCAG CGCGACTTCA CGCAGCACCA CCGCCTGCCC GCCGACGAGT TCTACGCGGA TTCGTTCACG AGCGCCGCGG ACCTCGCGCA TACGGTCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BupsA.17533.a.A1.PS01032
PURIFICATION DATe: 4/5/2011
CONCENTRATION: 31.19mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 18
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MREVRGARER IRVELVGGQA VVLREVALRG FDHHRRAARV HLMAGQVGQI LDHRAVHEAR AACPRVARVG LGKHRHELEV RDLLRPLLGG VEQIQIFLAA RAPVERHRAL ELHVVRVLDD RLDRREARAG GEQHDRLVRI LAQEERAERA FEAQDVALLH RAEHVVGELA ARHVADVQLD RTFLVRRGRH RVAAARAVLQ DELDVLAGQI LQALVRGQLQ LQHDHVVRAA LERRDATRQL LHLNAVDARD LAHVDLEIAL RLRAAQQREA ARALVVRQRR RGMRSLLELA LHDRALARAA RAVLAAVGQM DVLTQRRREH GFVGLDLELP AALLEGYVKS HRTRTLGLSF QGT
Validated NT Sequence
ttattagacc gtatgcgcga ggtccgcggc gctcgtgaac gaatccgcgt agaactcgtc ggcgggcagg cggtggtgct gcgtgaagtc gcgctgcgcg gattcgacca tcaccggcgc gccgcacgcg tacacctgat ggccggacag gtcgggcaga tcctcgatca ccgcgcggtg cacgaagccc gtgcggcctg tccacgcgtc gctcgcgtcg ggctcggaaa gcaccggcac gaacttgaag ttcgggatct ccttcgccca ttgctcggcg gcgtcgagca gatacagatc tttcttgcgg cgcgcgcccc agtagagcgt catcgggcgc tcgagcttca cgtggtgcgc gtgctcgatg atcgccttga tcggcgcgaa gcccgtgccg gaggcgagca gcacgatcgg cttgtccgaa tcctcgcgca ggaagaacgt gccgagcggg ccttcgaagc gcaggatgtc gcgctccttc atcgggccga acacgtggtc ggtgaacttg ccgcccggca tgtggcggat gtgcagctcg atcggacctt cctcgtgcgg cgcggtcgcc atcgagtagc tgcggcgcgc gccgtccttc aggatgaact cgatgtactg gccggccaga tactgcaggc gctcgttcgc gggcaactgc agcttcagca cgatcacgtc gtccgcgcgg cgctcgagcg ccgcgacgcg acacggcagc ttcttcacct gaacgccgtc gacgcccgcg atctcgcgca cgtcgacctc gagatcgctc tgcggcttcg agcagcacag cagcgcgagg ccgcgcgtgc gctcgtcgtt cgacagcgcc gacgcggaat gcggtccctg ctcgaactgg ccttgcacga tcgtgccctt gcacgagccg cacgcgccgt tcttgcagcc gtagggcaga tggacgttct gacgcagcgc cgccgcgagc acggtttcgt cggcctcgac ctggaactgc cggccgcttt gcttgagggt tacgttaaaa gccatcgaac caggaccctg ggtctgagct tccagggtac cc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFNVTLKQ SGRQFQVEAD ETVLAAALRQ NVHLPYGCKN GACGSCKGTI VQGQFEQGPH SASALSNDER TRGLALLCCS KPQSDLEVDV REIAGVDGVQ VKKLPCRVAA LERRADDVIV LKLQLPANER LQYLAGQYIE FILKDGARRS YSMATAPHEE GPIELHIRHM PGGKFTDHVF GPMKERDILR FEGPLGTFFL REDSDKPIVL LASGTGFAPI KAIIEHAHHV KLERPMTLYW GARRKKDLYL LDAAEQWAKE IPNFKFVPVL SEPDASDAWT GRTGFVHRAV IEDLPDLSGH QVYACGAPVM VESAQRDFTQ HHRLPADEFY ADSFTSAADL AHTV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTTTTAAC GTAACCCTCA AGCAAAGCGG CCGGCAGTTC CAGGTCGAGG CCGACGAAAC CGTGCTCGCG GCGGCGCTGC GTCAGAACGT CCATCTGCCC TACGGCTGCA AGAACGGCGC GTGCGGCTCG TGCAAGGGCA CGATCGTGCA AGGCCAGTTC GAGCAGGGAC CGCATTCCGC GTCGGCGCTG TCGAACGACG AGCGCACGCG CGGCCTCGCG CTGCTGTGCT GCTCGAAGCC GCAGAGCGAT CTCGAGGTCG ACGTGCGCGA GATCGCGGGC GTCGACGGCG TTCAGGTGAA GAAGCTGCCG TGTCGCGTCG CGGCGCTCGA GCGCCGCGCG GACGACGTGA TCGTGCTGAA GCTGCAGTTG CCCGCGAACG AGCGCCTGCA GTATCTGGCC GGCCAGTACA TCGAGTTCAT CCTGAAGGAC GGCGCGCGCC GCAGCTACTC GATGGCGACC GCGCCGCACG AGGAAGGTCC GATCGAGCTG CACATCCGCC ACATGCCGGG CGGCAAGTTC ACCGACCACG TGTTCGGCCC GATGAAGGAG CGCGACATCC TGCGCTTCGA AGGCCCGCTC GGCACGTTCT TCCTGCGCGA GGATTCGGAC AAGCCGATCG TGCTGCTCGC CTCCGGCACG GGCTTCGCGC CGATCAAGGC GATCATCGAG CACGCGCACC ACGTGAAGCT CGAGCGCCCG ATGACGCTCT ACTGGGGCGC GCGCCGCAAG AAAGATCTGT ATCTGCTCGA CGCCGCCGAG CAATGGGCGA AGGAGATCCC GAACTTCAAG TTCGTGCCGG TGCTTTCCGA GCCCGACGCG AGCGACGCGT GGACAGGCCG CACGGGCTTC GTGCACCGCG CGGTGATCGA GGATCTGCCC GACCTGTCCG GCCATCAGGT GTACGCGTGC GGCGCGCCGG TGATGGTCGA ATCCGCGCAG CGCGACTTCA CGCAGCACCA CCGCCTGCCC GCCGACGAGT TCTACGCGGA TTCGTTCACG AGCGCCGCGG ACCTCGCGCA TACGGTCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA