SSGCID: Target Page

BupsA.17527.a

Putaive vitamin B12 transport protein

CENTER ID:	BupsA.17527.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17527.a.A2.GE31326	Mature protein( BupsA.17527.a )		49	332

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17527.a.A2.PS01043	Mature protein( BupsA.17527.a )		49	332

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.1207
RefSeq:	YP_332607.1
UniProt:	Q3JUZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRADASGRSR ASFRIDHTGL TPMRPALRRA ARIALAALFC ASACAHALAA AHADIAVTDD AGHAITLAAP ARRVVSLAPH VTELIYAAGG GAKLVGAVSY SDYPPAAKAI ARVGSNKALD LERIAALKPD LIVVWRHGNA EHETERLRAL GIPLYFSEPR HLDDVAASLD KLGLLLGTHE IASAAADAYR RRIAQLRARY ADKPPVTVFF QAWDKPLITL NGDHIVSDVI ALCGGRNVFA RLQPVAPSVT DEAVLAANPE AIVTTAAGAS PTRDALPDFT RWRAWPKLTA VARGNLFAID GDLLTRPAPR IAQGAEQLCR DLDVARARRP KP

NT Sequence

GTGCGTGCCG ACGCTAGCGG CCGCAGCCGC GCATCCTTCC GAATCGACCA CACGGGATTG ACACCGATGC GCCCCGCCCT GCGCCGCGCC GCACGCATCG CACTCGCCGC CCTCTTCTGC GCGAGCGCAT GCGCGCATGC GCTCGCCGCC GCGCATGCGG ACATCGCCGT CACCGACGAC GCCGGGCACG CGATCACGCT CGCCGCGCCC GCACGGCGCG TCGTGAGCCT CGCGCCGCAC GTGACCGAGC TGATCTATGC GGCGGGCGGC GGCGCGAAGC TCGTCGGCGC GGTGTCGTAC AGCGACTACC CGCCCGCCGC GAAGGCGATT GCGCGCGTCG GCTCGAACAA GGCGCTCGAT CTCGAACGGA TCGCGGCGCT CAAGCCCGAT CTGATCGTCG TCTGGCGCCA CGGCAACGCC GAGCACGAGA CCGAGCGGCT GCGCGCGCTC GGCATTCCGC TTTACTTCAG CGAGCCACGG CATCTCGACG ATGTCGCCGC ATCGCTCGAC AAGCTCGGCC TGCTGCTCGG CACGCACGAG ATCGCGTCGG CCGCGGCCGA CGCGTACCGG CGGCGAATCG CGCAGCTGCG CGCGCGCTAC GCGGACAAGC CGCCCGTCAC GGTGTTCTTC CAGGCGTGGG ACAAACCGCT CATCACGCTG AACGGCGATC ACATCGTGAG CGACGTGATC GCGCTGTGCG GCGGGCGCAA CGTGTTCGCG CGGCTGCAGC CGGTCGCGCC GTCCGTCACC GACGAAGCGG TGCTCGCGGC AAATCCTGAG GCGATCGTGA CGACGGCGGC GGGCGCGAGC CCGACGCGCG ACGCACTGCC CGACTTCACG CGCTGGCGCG CATGGCCGAA GCTGACCGCG GTCGCGCGGG GCAATCTGTT CGCGATCGAC GGCGATCTGC TGACGCGGCC CGCGCCGCGC ATCGCGCAGG GCGCCGAGCA ACTGTGCCGG GATCTCGACG TCGCGCGCGC GAGACGGCCG AAGCCG

Details for BupsA.17527.a.A2.GE31326

HARVESTED ON:	1/26/2011
SEQUENCED ON:	1/31/2011
EXPECTED MW:	32kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMAAAHAD IAVTDDAGHA ITLAAPARRV VSLAPHVTEL IYAAGGGAKL VGAVSYSDYP PAAKAIARVG SNKALDLERI AALKPDLIVV WRHGNAEHET ERLRALGIPL YFSEPRHLDD VAASLDKLGL LLGTHEIASA AADAYRRRIA QLRARYADKP PVTVFFQAWD KPLITLNGDH IVSDVIALCG GRNVFARLQP VAPSVTDEAV LAANPEAIVT TAAGASPTRD ALPDFTRWRA WPKLTAVARG NLFAIDGDLL TRPAPRIAQG AEQLCRDLDV ARAR

Validated NT Sequence

gtctcgcgcg cgcgacgtcg agatcccggc acagttgctc ggcgccctgc gcgatgcgcg gcgcgggccg cgtcagcaga tcgccgtcga tcgcgaacag attgccccgc gcgaccgcgg tcagcttcgg ccatgcgcgc cagcgcgtga agtcgggcag tgcgtcgcgc gtcgggctcg cgcccgccgc cgtcgtcacg atcgcctcag gatttgccgc gagcaccgct tcgtcggtga cggacggcgc gaccggctgc agccgcgcga acacgttgcg cccgccgcac agcgcgatca cgtcgctcac gatgtgatcg ccgttcagcg tgatgagcgg tttgtcccac gcctggaaga acaccgtgac gggcggcttg tccgcgtagc gcgcgcgcag ctgcgcgatt cgccgccggt acgcgtcggc cgcggccgac gcgatctcgt gcgtgccgag cagcaggccg agcttgtcga gcgatgcggc gacatcgtcg agatgccgtg gctcgctgaa gtaaagcgga atgccgagcg cgcgcagccg ctcggtctcg tgctcggcgt tgccgtggcg ccagacgacg atcagatcgg gcttgagcgc cgcgatccgt tcgagatcga gcgccttgtt cgagccgacg cgcgcaatcg ccttcgcggc gggcgggtag tcgctgtacg acaccgcgcc gacgagcttc gcgccgccgc ccgccgcata gatcagctcg gtcacgtgcg gcgcgaggct cacgacgcgc cgtgcgggcg cggcgagcgt gatcgcgtgc ccggcgtcgt cggtgacggc gatgtccgca tgcgcggcgg ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAAAHADIA VTDDAGHAIT LAAPARRVVS LAPHVTELIY AAGGGAKLVG AVSYSDYPPA AKAIARVGSN KALDLERIAA LKPDLIVVWR HGNAEHETER LRALGIPLYF SEPRHLDDVA ASLDKLGLLL GTHEIASAAA DAYRRRIAQL RARYADKPPV TVFFQAWDKP LITLNGDHIV SDVIALCGGR NVFARLQPVA PSVTDEAVLA ANPEAIVTTA AGASPTRDAL PDFTRWRAWP KLTAVARGNL FAIDGDLLTR PAPRIAQGAE QLCRDLDVAR ARRPKP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgccgcg catgcggaca tcgccgtcac cgacgacgcc gggcacgcga tcacgctcgc cgcgcccgca cggcgcgtcg tgagcctcgc gccgcacgtg accgagctga tctatgcggc gggcggcggc gcgaagctcg tcggcgcggt gtcgtacagc gactacccgc ccgccgcgaa ggcgattgcg cgcgtcggct cgaacaaggc gctcgatctc gaacggatcg cggcgctcaa gcccgatctg atcgtcgtct ggcgccacgg caacgccgag cacgagaccg agcggctgcg cgcgctcggc attccgcttt acttcagcga gccacggcat ctcgacgatg tcgccgcatc gctcgacaag ctcggcctgc tgctcggcac gcacgagatc gcgtcggccg cggccgacgc gtaccggcgg cgaatcgcgc agctgcgcgc gcgctacgcg gacaagccgc ccgtcacggt gttcttccag gcgtgggaca aaccgctcat cacgctgaac ggcgatcaca tcgtgagcga cgtgatcgcg ctgtgcggcg ggcgcaacgt gttcgcgcgg ctgcagccgg tcgcgccgtc cgtcaccgac gaagcggtgc tcgcggcaaa tcctgaggcg atcgtgacga cggcggcggg cgcgagcccg acgcgcgacg cactgcccga cttcacgcgc tggcgcgcat ggccgaagct gaccgcggtc gcgcggggca atctgttcgc gatcgacggc gatctgctga cgcggcccgc gccgcgcatc gcgcagggcg ccgagcaact gtgccgggat ctcgacgtcg cgcgcgcgag acggccgaag ccgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BupsA.17527.a.A2.PS01043

PURIFICATION DATe:	3/29/2011
CONCENTRATION:	16.04mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGCCGCG CATGCGGACA TCGCCGTCAC CGACGACGCC GGGCACGCGA TCACGCTCGC CGCGCCCGCA CGGCGCGTCG TGAGCCTCGC GCCGCACGTG ACCGAGCTGA TCTATGCGGC GGGCGGCGGC GCGAAGCTCG TCGGCGCGGT GTCGTACAGC GACTACCCGC CCGCCGCGAA GGCGATTGCG CGCGTCGGCT CGAACAAGGC GCTCGATCTC GAACGGATCG CGGCGCTCAA GCCCGATCTG ATCGTCGTCT GGCGCCACGG CAACGCCGAG CACGAGACCG AGCGGCTGCG CGCGCTCGGC ATTCCGCTTT ACTTCAGCGA GCCACGGCAT CTCGACGATG TCGCCGCATC GCTCGACAAG CTCGGCCTGC TGCTCGGCAC GCACGAGATC GCGTCGGCCG CGGCCGACGC GTACCGGCGG CGAATCGCGC AGCTGCGCGC GCGCTACGCG GACAAGCCGC CCGTCACGGT GTTCTTCCAG GCGTGGGACA AACCGCTCAT CACGCTGAAC GGCGATCACA TCGTGAGCGA CGTGATCGCG CTGTGCGGCG GGCGCAACGT GTTCGCGCGG CTGCAGCCGG TCGCGCCGTC CGTCACCGAC GAAGCGGTGC TCGCGGCAAA TCCTGAGGCG ATCGTGACGA CGGCGGCGGG CGCGAGCCCG ACGCGCGACG CACTGCCCGA CTTCACGCGC TGGCGCGCAT GGCCGAAGCT GACCGCGGTC GCGCGGGGCA ATCTGTTCGC GATCGACGGC GATCTGCTGA CGCGGCCCGC GCCGCGCATC GCGCAGGGCG CCGAGCAACT GTGCCGGGAT CTCGACGTCG CGCGCGCGAG ACGGCCGAAG CCGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA