SSGCID: Target Page

BupsA.17519.a

Lipopolysaccharide heptosyltransferase II

CENTER ID:	BupsA.17519.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17519.a.A1.GE31325	Full length( BupsA.17519.a )		1	346

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.1001
RefSeq:	YP_332402.1
UniProt:	Q3JVK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRALVIAPN WIGDALMAQP LFALLKKLHP RIVIDAVAPA WVAPVLERMP EIHDVHATEL AHGKLQMLHR WQLASDLREL GYDAAYVLPN SLKSALIPWL AGIPLRIGYT GEHRYALLNV RHANPGKARE TRAPMAQHYA ALAYAPGAKL PESFQTLPPP RLDADLNETA RVSARFNLDT RKPLIVFCPG AEYGPAKRWP PEHFAALAQS VSQSFPYTQI VALGSPKDAA AAQAIAERAP NVRSLCGQTS LTEACALIAR ANAVVTNDSG LMHVAAALRR PLVALYGSTD PRHTPPLSEL AKVQWLHLEC SPCFERECPL GHLKCLRELS PEQVFGDLRG MLVGQR

NT Sequence

ATGCGTCGCG CGTTGGTTAT CGCACCGAAC TGGATCGGTG ACGCATTGAT GGCGCAGCCG CTTTTCGCGC TGCTGAAGAA ACTGCATCCC CGCATCGTCA TCGATGCCGT GGCGCCCGCG TGGGTCGCGC CCGTGCTCGA GCGGATGCCC GAAATCCACG ATGTCCACGC GACCGAGCTC GCGCACGGCA AGCTGCAGAT GCTGCACCGC TGGCAGCTCG CGAGCGACTT GCGCGAGCTC GGTTACGACG CCGCGTATGT GCTGCCGAAT TCGCTGAAAT CGGCGCTGAT TCCGTGGCTC GCCGGCATCC CGCTTCGGAT CGGCTACACG GGCGAGCATC GCTACGCGCT CCTGAACGTG CGGCATGCGA ATCCGGGCAA GGCGCGCGAG ACGCGCGCGC CGATGGCACA ACATTACGCG GCGCTTGCAT ACGCGCCCGG CGCGAAGCTG CCCGAATCGT TCCAGACGCT GCCGCCGCCG CGGCTCGATG CGGACCTGAA CGAGACGGCG CGCGTGTCGG CGCGCTTTAA CCTCGACACG CGCAAGCCGC TCATCGTGTT CTGTCCGGGC GCCGAGTACG GCCCGGCCAA GCGCTGGCCC CCCGAGCATT TCGCCGCGCT CGCGCAGAGC GTGAGCCAGT CCTTTCCGTA CACGCAGATC GTCGCGCTCG GCTCGCCGAA GGACGCCGCG GCCGCGCAGG CGATCGCCGA GCGCGCGCCG AACGTGCGCA GCCTCTGCGG CCAGACCTCG CTCACCGAGG CGTGCGCGCT GATCGCGCGC GCGAACGCGG TCGTCACGAA CGATTCCGGG CTGATGCACG TCGCGGCGGC GCTGCGCCGG CCGCTCGTCG CGCTCTACGG ATCGACCGAT CCGCGTCATA CCCCGCCGCT GTCGGAGCTG GCAAAGGTAC AATGGCTGCA TCTCGAATGC AGTCCCTGCT TCGAACGCGA ATGCCCGCTC GGCCACCTGA AGTGCCTGCG CGAGCTGAGC CCGGAGCAGG TGTTCGGCGA TTTGCGCGGG ATGCTCGTCG GACAGCGC

Details for BupsA.17519.a.A1.GE31325

HARVESTED ON:	1/26/2011
SEQUENCED ON:	1/31/2011
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ccgacgagca tcccgcgcaa atcgccgaac acctgctccg ggctcagctc gcgcaggcac ttcaggtggc cgagcgggca ttcgcgttcg aagcagggac tgcattcgag atgcagccat tgtacctttg ccagctccga cagcggcggg gtatgacgcg gatcggtcga tccgtagagc gcgacgagcg gccggcgcag cgccgccgcg acgtgcatca gcccggaatc gttcgtgacg accgcgttcg cgcgcgcgat cagcgcgcac gcctcggtga gcgaggtctg gccgcagagg ctgcgcacgt tcggcgcgcg ctcggcgatc gcctgcgcgg ccgcggcgtc cttcggcgag ccgagcgcga cgatctgcgt gtacggaaag gactggctca cgctctgcgc gagcgcggcg aaatgctcgg ggggccagcg cttggccggg ccgtactcgg cgcccggaca gaacacgatg agcggcttgc gcgtgtcgag gttaaagcgc gccgacacgc gcgccgtctc gttcaggtcc gcatcgagcc gcggcggcgg cagcgtctgg aacgattcgg gcagcttcgc gccgggcgcg tatgcaagcg ccgcgtaatg ttgtgccatc ggcgcgcgcg tctcgcgcgc cttgcccgga ttcgcatgcc gcacgttcag gagcgcgtag cgatgctcgc ccgtgtagcc gatccgaagc gggatgccgg cgagccacgg aatcagcgcc gatttcagcg aattcggcag cacatacgcg gcgtcgtaac cgagctcgcg caagtcgctc gcgagctgcc agcggtgcag catctgcagc ttgccgtgcg cgagctcggt cgcgtggaca tcgtggattt cgggcatccg ctcgagcacg ggcgcgaccc acgcgggcgc cacggcatcg atgacgatgc ggggatgcag tttcttcagc agcgcgaaaa gcggctgcgc catcaatgcg tcaccgatcc agttcggtgc gataaccaac gcgcgacgca tcgaaccagg accctgggtc tgagcttcca gggtaccc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRRALVIAP NWIGDALMAQ PLFALLKKLH PRIVIDAVAP AWVAPVLERM PEIHDVHATE LAHGKLQMLH RWQLASDLRE LGYDAAYVLP NSLKSALIPW LAGIPLRIGY TGEHRYALLN VRHANPGKAR ETRAPMAQHY AALAYAPGAK LPESFQTLPP PRLDADLNET ARVSARFNLD TRKPLIVFCP GAEYGPAKRW PPEHFAALAQ SVSQSFPYTQ IVALGSPKDA AAAQAIAERA PNVRSLCGQT SLTEACALIA RANAVVTNDS GLMHVAAALR RPLVALYGST DPRHTPPLSE LAKVQWLHLE CSPCFERECP LGHLKCLREL SPEQVFGDLR GMLVGQR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtcgcgcg ttggttatcg caccgaactg gatcggtgac gcattgatgg cgcagccgct tttcgcgctg ctgaagaaac tgcatccccg catcgtcatc gatgccgtgg cgcccgcgtg ggtcgcgccc gtgctcgagc ggatgcccga aatccacgat gtccacgcga ccgagctcgc gcacggcaag ctgcagatgc tgcaccgctg gcagctcgcg agcgacttgc gcgagctcgg ttacgacgcc gcgtatgtgc tgccgaattc gctgaaatcg gcgctgattc cgtggctcgc cggcatcccg cttcggatcg gctacacggg cgagcatcgc tacgcgctcc tgaacgtgcg gcatgcgaat ccgggcaagg cgcgcgagac gcgcgcgccg atggcacaac attacgcggc gcttgcatac gcgcccggcg cgaagctgcc cgaatcgttc cagacgctgc cgccgccgcg gctcgatgcg gacctgaacg agacggcgcg cgtgtcggcg cgctttaacc tcgacacgcg caagccgctc atcgtgttct gtccgggcgc cgagtacggc ccggccaagc gctggccccc cgagcatttc gccgcgctcg cgcagagcgt gagccagtcc tttccgtaca cgcagatcgt cgcgctcggc tcgccgaagg acgccgcggc cgcgcaggcg atcgccgagc gcgcgccgaa cgtgcgcagc ctctgcggcc agacctcgct caccgaggcg tgcgcgctga tcgcgcgcgc gaacgcggtc gtcacgaacg attccgggct gatgcacgtc gcggcggcgc tgcgccggcc gctcgtcgcg ctctacggat cgaccgatcc gcgtcatacc ccgccgctgt cggagctggc aaaggtacaa tggctgcatc tcgaatgcag tccctgcttc gaacgcgaat gcccgctcgg ccacctgaag tgcctgcgcg agctgagccc ggagcaggtg ttcggcgatt tgcgcgggat gctcgtcgga cagcgctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa