SSGCID: Target Page

BupsA.17518.a

Universal stress protein family domain protein

CENTER ID:	BupsA.17518.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17518.a.A1.GE31324	Full length( BupsA.17518.a )		1	279

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17518.a.A1.PS01027	Full length( BupsA.17518.a )		1	279

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.6638
RefSeq:	YP_337581.1
UniProt:	Q3JFS2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSYKSILVHL DTSDRARARL ETALTLARQF GAYLSAVFAV YTPEPTSFYV MAGTADYFAD QQRRRDEKRA ALERLFHAEL KRADVEGQWI VADARANEAV PHYARYADLV IAGQTDPDDP ETYVDDSFPD TLVLSAGRPV LLLPYAGMPS AIGTRVLVAW DGSREATRAV HDAAPFLALA TKTTIVTVNG AAHEPPGARI PGADIALTLA RHHANIDVRD LERARDASIG DVLLSHAYES GTDLLVMGAY GHARWKELIL GGVTRTIFAS MTVPVLMSH

NT Sequence

ATGAGCTACA AGAGCATCCT GGTCCACCTC GACACGAGCG ATCGCGCACG CGCGCGGCTC GAGACCGCGC TGACGCTCGC GCGCCAGTTC GGCGCGTACC TGAGCGCGGT CTTCGCCGTC TACACCCCCG AGCCGACGTC GTTCTACGTG ATGGCGGGCA CCGCCGACTA CTTCGCCGAT CAGCAAAGGC GGCGCGACGA GAAGCGCGCG GCGCTCGAGC GCCTGTTCCA CGCCGAACTC AAGCGCGCGG ACGTCGAGGG CCAGTGGATC GTCGCCGACG CGCGCGCGAA CGAAGCGGTG CCGCATTACG CGCGCTACGC GGATCTCGTG ATCGCCGGCC AGACCGATCC GGACGATCCG GAAACCTATG TCGACGACAG CTTCCCCGAC ACGCTCGTGC TGTCGGCCGG CCGCCCGGTG CTGCTGCTGC CGTACGCGGG CATGCCGTCG GCGATCGGCA CGCGCGTGCT CGTCGCGTGG GACGGCAGCC GCGAGGCGAC GCGCGCCGTT CACGACGCGG CGCCGTTCCT CGCGCTCGCG ACGAAGACGA CGATCGTCAC CGTGAACGGC GCCGCGCACG AGCCGCCCGG CGCGCGAATT CCGGGCGCCG ACATCGCGCT CACGCTCGCG CGGCATCACG CGAACATCGA CGTGCGCGAC CTCGAACGCG CGCGCGACGC GTCGATCGGC GACGTGCTGC TGTCGCACGC GTACGAATCG GGCACCGATC TGCTCGTGAT GGGCGCATAC GGCCACGCGC GCTGGAAGGA GCTGATTCTC GGCGGCGTGA CGCGCACGAT CTTTGCGTCG ATGACGGTGC CGGTGCTGAT GTCGCAC

Details for BupsA.17518.a.A1.GE31324

HARVESTED ON:	1/26/2011
SEQUENCED ON:	1/31/2011
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMSYKSIL VHLDTSDRAR ARLETALTLA RQFGAYLSAV FAVYTPEPTS FYVMAGTADY FADQQRRRDE KRAALERLFH AELKRADVEG QWIVADARAN EAVPHYARYA DLVIAGQTDP DDPETYVDDS FPDTLVLSAG RPVLLLPYAG MPSAIGTRVL VAWXGSREAT RAVHDAAPFL ALATKTTIVT VNGAAHEPPG ARIPGADXAL TLARHHANIX VRDLERARDA SIGDVLLSHA YESGTDLLVM GAYGHARWKE LILGGVTRTI FASMTVPVLM SH

Validated NT Sequence

ttattagtgc gacatcagca ccggcaccgt catcgacgca aagatcgtgc gcgtcacgcc gccgagaatc agctccttcc agcgcgcgtg gccgtatgcg cccatcacga gcagatcggt gcccgattcg tacgcgtgcg acagcagcac gtcgccgatc gacgcgtcgc gcgcgcgttc gaggtcgcgc acgnngatgt tcgcgtgatg ccgcgcgagc gtgagcgcga ngtcggcgcc cggaattcgc gcgccgggcg gctcgtgcgc ggcgccgttc acggtgacga tcgtngtctt ngtcgcgagc gcgaggaacg gcgccgcgtc gtgaacggcg cgcgtcgcct cgcggctgcc ntcccacgcg acgagcacgc gcgtgccgat cgcngacggc atgcccgcgt acggcagcag cagcaccggg cggccggccg acagcacgag cgtgtcgggg aagctgtcgt cgacataggt ttccggatcg tccggatcgg tctggccggc gatcacgaga tccgcgtagc gcgcgtaatg cggcaccgct tcgttcgcgc gcgcgtcggc gacgatccac tggccctcga cgtccgcgcg cttgagttcg gcgtggaaca ggcgctcgag cgccgcgcgc ttctcgtcgc gccgcctttg ctgatcggcg aagtagtcgg cggtgcccgc catcacgtag aacgacgtcg gctcgggggt gtagacggcg aagaccgcgc tcaggtacgc gccgaactgg cgcgcgagcg tcagcgcggt ctcgagccgc gcgcgtgcgc gatcgctcgt gtcgaggtgg accaggatgc tcttgtagct catcgaacca ggaccctggg tctgagcttc cagggtaccc at

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSYKSILVH LDTSDRARAR LETALTLARQ FGAYLSAVFA VYTPEPTSFY VMAGTADYFA DQQRRRDEKR AALERLFHAE LKRADVEGQW IVADARANEA VPHYARYADL VIAGQTDPDD PETYVDDSFP DTLVLSAGRP VLLLPYAGMP SAIGTRVLVA WDGSREATRA VHDAAPFLAL ATKTTIVTVN GAAHEPPGAR IPGADIALTL ARHHANIDVR DLERARDASI GDVLLSHAYE SGTDLLVMGA YGHARWKELI LGGVTRTIFA SMTVPVLMSH

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagctacaag agcatcctgg tccacctcga cacgagcgat cgcgcacgcg cgcggctcga gaccgcgctg acgctcgcgc gccagttcgg cgcgtacctg agcgcggtct tcgccgtcta cacccccgag ccgacgtcgt tctacgtgat ggcgggcacc gccgactact tcgccgatca gcaaaggcgg cgcgacgaga agcgcgcggc gctcgagcgc ctgttccacg ccgaactcaa gcgcgcggac gtcgagggcc agtggatcgt cgccgacgcg cgcgcgaacg aagcggtgcc gcattacgcg cgctacgcgg atctcgtgat cgccggccag accgatccgg acgatccgga aacctatgtc gacgacagct tccccgacac gctcgtgctg tcggccggcc gcccggtgct gctgctgccg tacgcgggca tgccgtcggc gatcggcacg cgcgtgctcg tcgcgtggga cggcagccgc gaggcgacgc gcgccgttca cgacgcggcg ccgttcctcg cgctcgcgac gaagacgacg atcgtcaccg tgaacggcgc cgcgcacgag ccgcccggcg cgcgaattcc gggcgccgac atcgcgctca cgctcgcgcg gcatcacgcg aacatcgacg tgcgcgacct cgaacgcgcg cgcgacgcgt cgatcggcga cgtgctgctg tcgcacgcgt acgaatcggg caccgatctg ctcgtgatgg gcgcatacgg ccacgcgcgc tggaaggagc tgattctcgg cggcgtgacg cgcacgatct ttgcgtcgat gacggtgccg gtgctgatgt cgcactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BupsA.17518.a.A1.PS01027

PURIFICATION DATe:	4/6/2011
CONCENTRATION:	10.57mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCTACAAG AGCATCCTGG TCCACCTCGA CACGAGCGAT CGCGCACGCG CGCGGCTCGA GACCGCGCTG ACGCTCGCGC GCCAGTTCGG CGCGTACCTG AGCGCGGTCT TCGCCGTCTA CACCCCCGAG CCGACGTCGT TCTACGTGAT GGCGGGCACC GCCGACTACT TCGCCGATCA GCAAAGGCGG CGCGACGAGA AGCGCGCGGC GCTCGAGCGC CTGTTCCACG CCGAACTCAA GCGCGCGGAC GTCGAGGGCC AGTGGATCGT CGCCGACGCG CGCGCGAACG AAGCGGTGCC GCATTACGCG CGCTACGCGG ATCTCGTGAT CGCCGGCCAG ACCGATCCGG ACGATCCGGA AACCTATGTC GACGACAGCT TCCCCGACAC GCTCGTGCTG TCGGCCGGCC GCCCGGTGCT GCTGCTGCCG TACGCGGGCA TGCCGTCGGC GATCGGCACG CGCGTGCTCG TCGCGTGGGA CGGCAGCCGC GAGGCGACGC GCGCCGTTCA CGACGCGGCG CCGTTCCTCG CGCTCGCGAC GAAGACGACG ATCGTCACCG TGAACGGCGC CGCGCACGAG CCGCCCGGCG CGCGAATTCC GGGCGCCGAC ATCGCGCTCA CGCTCGCGCG GCATCACGCG AACATCGACG TGCGCGACCT CGAACGCGCG CGCGACGCGT CGATCGGCGA CGTGCTGCTG TCGCACGCGT ACGAATCGGG CACCGATCTG CTCGTGATGG GCGCATACGG CCACGCGCGC TGGAAGGAGC TGATTCTCGG CGGCGTGACG CGCACGATCT TTGCGTCGAT GACGGTGCCG GTGCTGATGT CGCACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA