SSGCID: Target Page

BupsA.17517.a

Putative uncharacterized protein

CENTER ID:	BupsA.17517.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17517.a.A1.GE31323	Full length( BupsA.17517.a )		1	314

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.3403
RefSeq:	YP_334637.1
UniProt:	Q3JP66

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKVFILHPGK ANYPEIAAYR DFLGPRGFDV HDGDASAYAR FDGRHDCILW CIMGFYRALP PARFVIHDYR SLSVGRLAAL KDTVKRAWNA KPALRIFQNE RMRDAMAFRD GIPSLLLPMG VPDWIFGIAN EPAGAGPAGR FCYIGEMSRE RGFHKVLAAY RAAPRTARDS LVLVGQPEPE IYEAFRHVEG LVFVGRVPQR DALKIVQRSD YAVCYFPYHR PHRFQTPTKL LEYAALGKKI ICNDAPSNVD AINALGIRSH VTGAAIFDEL LPLAQVRAQR NDPARLKRLE WTAVIARSGV LAHIEAAASM RGVR

NT Sequence

ATGAAAGTAT TCATCCTTCA CCCGGGGAAG GCGAATTACC CGGAAATCGC CGCGTACCGC GACTTTCTCG GCCCGCGCGG CTTCGACGTC CACGATGGCG ACGCGAGCGC GTATGCGCGG TTCGATGGGC GACACGATTG CATTCTGTGG TGCATCATGG GCTTTTATCG CGCGTTGCCG CCCGCGCGGT TCGTGATTCA CGATTACCGG TCGCTGTCGG TCGGCCGGCT CGCGGCGCTC AAGGATACGG TCAAGCGTGC GTGGAACGCA AAGCCGGCTC TGCGGATTTT CCAGAACGAG CGCATGCGCG ACGCGATGGC GTTTCGCGAC GGCATACCGT CGCTGCTGCT GCCGATGGGC GTGCCGGACT GGATCTTCGG GATCGCGAAC GAACCGGCGG GCGCGGGCCC GGCCGGCCGG TTCTGCTACA TCGGCGAGAT GAGCCGCGAG CGCGGCTTCC ACAAGGTGTT GGCCGCGTAT CGTGCCGCGC CGCGCACGGC GCGCGACTCG CTCGTGCTCG TCGGGCAGCC GGAGCCGGAG ATCTACGAGG CGTTCAGACA TGTCGAGGGC CTCGTGTTCG TCGGCCGCGT GCCGCAGCGC GATGCGCTCA AGATCGTGCA ACGCTCCGAC TATGCGGTTT GCTATTTTCC GTACCACCGT CCACACCGCT TCCAGACGCC GACGAAGCTG CTCGAGTACG CGGCGCTCGG CAAGAAGATC ATTTGCAACG ACGCGCCGTC GAATGTCGAT GCGATCAACG CGCTCGGCAT CCGCTCGCAT GTCACGGGCG CGGCGATATT CGACGAGTTG CTGCCGCTCG CGCAGGTGCG CGCGCAGCGC AACGATCCCG CGCGGCTGAA GCGCCTCGAA TGGACGGCCG TGATCGCGCG ATCGGGCGTG CTCGCGCATA TCGAGGCGGC GGCCTCGATG CGAGGTGTGC GA

Details for BupsA.17517.a.A1.GE31323

HARVESTED ON:	1/26/2011
SEQUENCED ON:	1/31/2011
EXPECTED MW:	37kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	76
PERCENT COVERAGE:	51

Validated AA Sequence

MXXXXXXXXX XXXXXMGXLX AQTQGPGSMK VFILHPGKAN YPEIAAYRDF LGPRGFDVHD GDASAYARFD GRHDCILWCI MGFYRALPPA RFVIXDYRSL SVGRLAALKD TVXRAWNAKP ALRIFQNERM XDAMAFRDXY RRCCXRWACR XGSSGSRTNR XARARPAGSA TSXR

Validated NT Sequence

gnnnnnncca tgtnnnnnnn nnntnnctnt ngnncnnnnn nnnnnnnnna tnanatgggt nccctggnag ctcagaccca gggtcctggt tcgatgaaag tattcatcct tcacccgggg aaggcgaatt acccggaaat cgccgcgtac cgcgactttc tcggcccgcg cggcttcgac gtccacgatg gcgacgcgag cgcgtatgcg cggttcgatg ggcgacacga ttgcattctg tggtgcatca tgggctttta tcgcgcgttg ccgcccgcgc ggttcgtgat tcncgattac cggtcgctgt cggtcggccg gctcgcggcg ctcaaggata cggtcaancg tgcgtggaac gcaaagccgg ctctgcggat tttccagaac gagcgcatgc ncgacgcgat ggcgtttcgc gacgnatacc gtcgctgctg ctgncgatgg gcgtgccgga ntggatcttc nggatcgcga acgaaccggn gggcgcgggc ccggccggcc ggttctgcta catcnnngag atgagncgng agcgcggctt ccacaaggtg ttgnccggng ccnnnncgcg cacggcgcgc gactcgctcg tgctncnncn ngcaancnga nnnnnanatc tacgangcgt tcagacntgt cgagngcctc nngttcntcn gnngcgtgnc gcnncgcgat gcgctnnana nnnngnnann ctccgacnat nngntttgct atnttccgta ccnnnnnnnn nnncgnntnc cngangncnn nnnnnnnnnn tcnannnnnn nnnntnnnnn anaanannnn nngngacncg nnnnnaannn nnnncnnnnn nncnct

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKVFILHPG KANYPEIAAY RDFLGPRGFD VHDGDASAYA RFDGRHDCIL WCIMGFYRAL PPARFVIHDY RSLSVGRLAA LKDTVKRAWN AKPALRIFQN ERMRDAMAFR DGIPSLLLPM GVPDWIFGIA NEPAGAGPAG RFCYIGEMSR ERGFHKVLAA YRAAPRTARD SLVLVGQPEP EIYEAFRHVE GLVFVGRVPQ RDALKIVQRS DYAVCYFPYH RPHRFQTPTK LLEYAALGKK IICNDAPSNV DAINALGIRS HVTGAAIFDE LLPLAQVRAQ RNDPARLKRL EWTAVIARSG VLAHIEAAAS MRGVR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagtattc atccttcacc cggggaaggc gaattacccg gaaatcgccg cgtaccgcga ctttctcggc ccgcgcggct tcgacgtcca cgatggcgac gcgagcgcgt atgcgcggtt cgatgggcga cacgattgca ttctgtggtg catcatgggc ttttatcgcg cgttgccgcc cgcgcggttc gtgattcacg attaccggtc gctgtcggtc ggccggctcg cggcgctcaa ggatacggtc aagcgtgcgt ggaacgcaaa gccggctctg cggattttcc agaacgagcg catgcgcgac gcgatggcgt ttcgcgacgg cataccgtcg ctgctgctgc cgatgggcgt gccggactgg atcttcggga tcgcgaacga accggcgggc gcgggcccgg ccggccggtt ctgctacatc ggcgagatga gccgcgagcg cggcttccac aaggtgttgg ccgcgtatcg tgccgcgccg cgcacggcgc gcgactcgct cgtgctcgtc gggcagccgg agccggagat ctacgaggcg ttcagacatg tcgagggcct cgtgttcgtc ggccgcgtgc cgcagcgcga tgcgctcaag atcgtgcaac gctccgacta tgcggtttgc tattttccgt accaccgtcc acaccgcttc cagacgccga cgaagctgct cgagtacgcg gcgctcggca agaagatcat ttgcaacgac gcgccgtcga atgtcgatgc gatcaacgcg ctcggcatcc gctcgcatgt cacgggcgcg gcgatattcg acgagttgct gccgctcgcg caggtgcgcg cgcagcgcaa cgatcccgcg cggctgaagc gcctcgaatg gacggccgtg atcgcgcgat cgggcgtgct cgcgcatatc gaggcggcgg cctcgatgcg aggtgtgcga taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa