SSGCID: Target Page

BupsA.17483.a

Cof-like hydrolase

CENTER ID:	BupsA.17483.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17483.a.A1.GE31310	Full length( BupsA.17483.a )		1	280

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.83
RefSeq:	YP_331503.1
UniProt:	Q3JY50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHPPMSAMYK AIATDLDGTL LNSDHQLDPY TIDTVRRLAG SGVPFVIATG RHYADVAGIR DLLGIRPYLI TSNGARVHGP DDTRIHAQDV PADAVRQLVR PELVGTHGRV IVNLFTNDGW LIDRDAPQLL AFHQDSGFRY EIVDTLAHDG ADIAKVLYIG EPEDLAVVSG NLARRFGDAL YVTYSLPDCL EVMTANVSKG RALRVVLERL GVDPAHCVAF GDNMNDIDLL ETAGYPFMMN NANPDLALRL PKVPRIGNNF EAGVARHLRA LFALEDSIAH

NT Sequence

TTGCATCCTC CGATGTCCGC CATGTATAAA GCCATCGCCA CCGATCTCGA CGGAACCCTG CTCAATAGCG ACCACCAGCT CGATCCTTAT ACGATCGACA CCGTGCGGCG GCTCGCGGGC AGCGGCGTGC CCTTCGTGAT CGCGACGGGG CGCCACTATG CGGATGTCGC GGGTATTCGC GACCTGCTCG GCATCCGTCC TTACCTGATC ACGTCGAACG GCGCGCGCGT GCACGGGCCG GACGACACGC GGATCCACGC GCAGGACGTG CCCGCCGACG CGGTGCGGCA ACTGGTGCGG CCCGAGCTCG TCGGCACGCA TGGCCGGGTG ATCGTCAATC TGTTCACGAA CGACGGCTGG CTGATCGATC GCGATGCGCC GCAACTGCTC GCATTCCATC AGGATTCCGG ATTTCGCTAC GAAATCGTCG ATACGCTGGC GCACGACGGC GCGGACATTG CGAAAGTGCT GTACATCGGC GAGCCCGAGG ATCTGGCCGT CGTCTCGGGC AATCTCGCGC GCCGGTTCGG CGACGCGCTG TACGTCACGT ATTCGCTGCC CGATTGCCTT GAGGTGATGA CGGCGAATGT ATCGAAGGGG CGCGCGCTGC GCGTCGTGCT CGAGCGGCTC GGCGTCGATC CCGCCCACTG CGTGGCGTTC GGCGACAACA TGAATGATAT CGACCTGCTC GAGACGGCCG GGTATCCGTT CATGATGAAC AACGCGAATC CCGACCTCGC CTTGCGCCTG CCGAAGGTGC CGCGGATCGG CAACAACTTC GAAGCGGGCG TCGCGCGCCA TCTGCGCGCG CTGTTCGCGC TCGAGGATTC CATCGCGCAC

Details for BupsA.17483.a.A1.GE31310

HARVESTED ON:	1/26/2011
SEQUENCED ON:	1/31/2011
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMLHPPMS AMYKAIATDL DGTLLNSDHQ LDPYTIDTVR RLAGSGVPFV IATGRHYADV AGIRDLLGIR PYLITSNGAR VHGPDDTRIH AQDVPADAVR QLVRPELVGT HGRVIVNLFT NDGWLIXRDA PQLLAFHQDS GFRYEIVDTL AHDGADIAKV LYIGEPEDLA VVSGNLARRF GDALYVTYSL PDCLEVMTAN VSKGRALRVV LERLGVDPAH CVAFGDNMND IDLLETAGYP FMMNNANPDL ALRLPKVPRI GNNFEAGVAR HLRALFALED SIAH

Validated NT Sequence

ttattagtgc gcgatggaat cctcgagcgc gaacagcgcg cgcagatggc gcgcgacgcc cgcttcgaag ttgttgccga tccgcggcac cttcggcagg cgcaaggcga ggtcgggatt cgcgttgttc atcatgaacg gatacccggc cgtctcgagc aggtcgatat cattcatgtt gtcgccgaac gccacgcagt gggcgggatc gacgccgagc cgctcgagca cgacgcgcag cgcgcgcccc ttcgatacat tcgccgtcat cacctcaagg caatcgggca gcgaatacgt gacgtacagc gcgtcgccga accggcgcgc gagattgccc gagacgacgg ccagatcctc gggctcgccg atgtacagca ctttcgcaat gtccgcgccg tcgtgcgcca gcgtatcgac gatttcgtag cgaaatccgg aatcctgatg gaatgcgagc agttgcggcg catcgcgatn gatcagccag ccgtcgttcg tgaacagatt gacgatcacc cggccatgcg tgccgacgag ctcgggccgc accagttgcc gcaccgcgtc ggcgggcacg tcctgcgcgt ggatccgcgt gtcgtccggc ccgtgcacgc gcgcgccgtt cgacgtgatc aggtaaggac ggatgccgag caggtcgcga atacccgcga catccgcata gtggcgcccc gtcgcgatca cgaagggcac gccgctgccc gcgagccgcc gcacggtgtc gatcgtataa ggatcgagct ggtggtcgct attgagcagg gttccgtcga gatcggtggc gatggcttta tacatggcgg acatcggagg atgcaacatc gaaccaggac cctgggtctg agcttccagg gtacccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLHPPMSAM YKAIATDLDG TLLNSDHQLD PYTIDTVRRL AGSGVPFVIA TGRHYADVAG IRDLLGIRPY LITSNGARVH GPDDTRIHAQ DVPADAVRQL VRPELVGTHG RVIVNLFTND GWLIDRDAPQ LLAFHQDSGF RYEIVDTLAH DGADIAKVLY IGEPEDLAVV SGNLARRFGD ALYVTYSLPD CLEVMTANVS KGRALRVVLE RLGVDPAHCV AFGDNMNDID LLETAGYPFM MNNANPDLAL RLPKVPRIGN NFEAGVARHL RALFALEDSI AH

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcatcct ccgatgtccg ccatgtataa agccatcgcc accgatctcg acggaaccct gctcaatagc gaccaccagc tcgatcctta tacgatcgac accgtgcggc ggctcgcggg cagcggcgtg cccttcgtga tcgcgacggg gcgccactat gcggatgtcg cgggtattcg cgacctgctc ggcatccgtc cttacctgat cacgtcgaac ggcgcgcgcg tgcacgggcc ggacgacacg cggatccacg cgcaggacgt gcccgccgac gcggtgcggc aactggtgcg gcccgagctc gtcggcacgc atggccgggt gatcgtcaat ctgttcacga acgacggctg gctgatcgat cgcgatgcgc cgcaactgct cgcattccat caggattccg gatttcgcta cgaaatcgtc gatacgctgg cgcacgacgg cgcggacatt gcgaaagtgc tgtacatcgg cgagcccgag gatctggccg tcgtctcggg caatctcgcg cgccggttcg gcgacgcgct gtacgtcacg tattcgctgc ccgattgcct tgaggtgatg acggcgaatg tatcgaaggg gcgcgcgctg cgcgtcgtgc tcgagcggct cggcgtcgat cccgcccact gcgtggcgtt cggcgacaac atgaatgata tcgacctgct cgagacggcc gggtatccgt tcatgatgaa caacgcgaat cccgacctcg ccttgcgcct gccgaaggtg ccgcggatcg gcaacaactt cgaagcgggc gtcgcgcgcc atctgcgcgc gctgttcgcg ctcgaggatt ccatcgcgca ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa