BupsA.17478.a
Chitin binding domain
| CENTER ID: | BupsA.17478.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.17478.a.A1.GE31308 | Full length( BupsA.17478.a ) | 1 | 214 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.17478.a.A1.PW31202 | Full length( BupsA.17478.a ) | 1 | 214 |
Structures
3UAM
DEPOSITED: 10/21/2011
DETERMINATION: XRay
CLONE: BupsA.17478.a.A1.GE31308
PROTEIN: BupsA.17478.a.A1.PW31202
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_153512 |
| PATRIC ID: | fig|320372.6.peg.113 |
| RefSeq: | YP_331531.1 |
| UniProt: | Q3JY22 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTTDSRSAT KSSSISPRHG RVITPESRAV YLYEAGRLDF GQVNELEGGK FFPATQSGLR DPDAPDDVAN GMPPRDGEIA SGGRTADARA QLNEPDSVAH WQKHAVRSGQ SLQISWSYSM PHKTRRWTYW ITKPGWDTQA RLARAHFEPD PLKVYLNTYQ PYWGPDADKE LIPQGETIHE FNLPTRTGYH VLLAVWDVAD TANAFYQVID LNFA
NT Sequence
ATGAGCACTA CCGATTCGAG AAGCGCAACG AAGTCTTCGT CCATTTCTCC GCGGCATGGC CGCGTCATCA CGCCCGAGTC GCGCGCCGTG TATCTCTACG AAGCAGGAAG GCTCGATTTC GGGCAGGTCA ATGAGCTGGA GGGCGGCAAA TTCTTTCCCG CGACCCAAAG CGGGCTCAGA GATCCGGACG CGCCGGACGA CGTCGCCAAC GGCATGCCGC CGCGCGACGG CGAAATCGCG AGCGGCGGGC GCACGGCGGA TGCCCGCGCG CAACTCAACG AACCCGATTC CGTTGCGCAT TGGCAGAAAC ATGCGGTGCG ATCGGGGCAA TCGCTGCAAA TCTCGTGGTC GTATTCGATG CCGCACAAGA CGCGGCGCTG GACTTACTGG ATCACGAAGC CGGGATGGGA CACCCAGGCG CGGCTTGCGC GCGCGCATTT CGAGCCCGAT CCGCTGAAGG TCTACCTGAA CACGTATCAG CCGTACTGGG GGCCGGATGC GGACAAGGAG TTGATCCCCC AGGGCGAGAC GATTCATGAA TTCAACCTGC CGACTCGCAC CGGCTATCAC GTACTGCTCG CGGTGTGGGA CGTCGCCGAT ACCGCCAACG CGTTCTATCA GGTGATCGAT CTGAATTTCG CG
Details for BupsA.17478.a.A1.GE31308
| HARVESTED ON: | 1/26/2011 |
| SEQUENCED ON: | 1/31/2011 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTTDSRSA TKSSSISPRH GRVITPESRA VYLYEAGRLD FGQVNELEGG KFFPATQSGL RDPDAPDDVA NGMPPRDGEI ASGGRTADAR AQLNEPDSVA HWQKHAVRSG QSLQISWSYS MPHKTRRWTY WITKPGWDTQ ARLARAHFEP DPLKVYLNTY QPYWGPDADK ELIPQGETIH EFNLPTRTGY HVLLAVWDVA DTANAFYQVI DLNFA
Validated NT Sequence
ttattacgcg aaattcagat cgatcacctg atagaacgcg ttggcggtat cggcgacgtc ccacaccgcg agcagtacgt gatagccggt gcgagtcggc aggttgaatt catgaatcgt ctcgccctgg gggatcaact ccttgtccgc atccggcccc cagtacggct gatacgtgtt caggtagacc ttcagcggat cgggctcgaa atgcgcgcgc gcaagccgcg cctgggtgtc ccatcccggc ttcgtgatcc agtaagtcca gcgccgcgtc ttgtgcggca tcgaatacga ccacgagatt tgcagcgatt gccccgatcg caccgcatgt ttctgccaat gcgcaacgga atcgggttcg ttgagttgcg cgcgggcatc cgccgtgcgc ccgccgctcg cgatttcgcc gtcgcgcggc ggcatgccgt tggcgacgtc gtccggcgcg tccggatctc tgagcccgct ttgggtcgcg ggaaagaatt tgccgccctc cagctcattg acctgcccga aatcgagcct tcctgcttcg tagagataca cggcgcgcga ctcgggcgtg atgacgcggc catgccgcgg agaaatggac gaagacttcg ttgcgcttct cgaatcggta gtgctcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTTDSRSA TKSSSISPRH GRVITPESRA VYLYEAGRLD FGQVNELEGG KFFPATQSGL RDPDAPDDVA NGMPPRDGEI ASGGRTADAR AQLNEPDSVA HWQKHAVRSG QSLQISWSYS MPHKTRRWTY WITKPGWDTQ ARLARAHFEP DPLKVYLNTY QPYWGPDADK ELIPQGETIH EFNLPTRTGY HVLLAVWDVA DTANAFYQVI DLNFA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcactacc gattcgagaa gcgcaacgaa gtcttcgtcc atttctccgc ggcatggccg cgtcatcacg cccgagtcgc gcgccgtgta tctctacgaa gcaggaaggc tcgatttcgg gcaggtcaat gagctggagg gcggcaaatt ctttcccgcg acccaaagcg ggctcagaga tccggacgcg ccggacgacg tcgccaacgg catgccgccg cgcgacggcg aaatcgcgag cggcgggcgc acggcggatg cccgcgcgca actcaacgaa cccgattccg ttgcgcattg gcagaaacat gcggtgcgat cggggcaatc gctgcaaatc tcgtggtcgt attcgatgcc gcacaagacg cggcgctgga cttactggat cacgaagccg ggatgggaca cccaggcgcg gcttgcgcgc gcgcatttcg agcccgatcc gctgaaggtc tacctgaaca cgtatcagcc gtactggggg ccggatgcgg acaaggagtt gatcccccag ggcgagacga ttcatgaatt caacctgccg actcgcaccg gctatcacgt actgctcgcg gtgtgggacg tcgccgatac cgccaacgcg ttctatcagg tgatcgatct gaatttcgcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BupsA.17478.a.A1.PW31202
| PURIFICATION DATe: | 4/29/2011 |
| CONCENTRATION: | 42.69mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTTDSRSA TKSSSISPRH GRVITPESRA VYLYEAGRLD FGQVNELEGG KFFPATQSGL RDPDAPDDVA NGMPPRDGEI ASGGRTADAR AQLNEPDSVA HWQKHAVRSG QSLQISWSYS MPHKTRRWTY WITKPGWDTQ ARLARAHFEP DPLKVYLNTY QPYWGPDADK ELIPQGETIH EFNLPTRTGY HVLLAVWDVA DTANAFYQVI DLNFA
Validated NT Sequence
ttattacgcg aaattcagat cgatcacctg atagaacgcg ttggcggtat cggcgacgtc ccacaccgcg agcagtacgt gatagccggt gcgagtcggc aggttgaatt catgaatcgt ctcgccctgg gggatcaact ccttgtccgc atccggcccc cagtacggct gatacgtgtt caggtagacc ttcagcggat cgggctcgaa atgcgcgcgc gcaagccgcg cctgggtgtc ccatcccggc ttcgtgatcc agtaagtcca gcgccgcgtc ttgtgcggca tcgaatacga ccacgagatt tgcagcgatt gccccgatcg caccgcatgt ttctgccaat gcgcaacgga atcgggttcg ttgagttgcg cgcgggcatc cgccgtgcgc ccgccgctcg cgatttcgcc gtcgcgcggc ggcatgccgt tggcgacgtc gtccggcgcg tccggatctc tgagcccgct ttgggtcgcg ggaaagaatt tgccgccctc cagctcattg acctgcccga aatcgagcct tcctgcttcg tagagataca cggcgcgcga ctcgggcgtg atgacgcggc catgccgcgg agaaatggac gaagacttcg ttgcgcttct cgaatcggta gtgctcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTTDSRSA TKSSSISPRH GRVITPESRA VYLYEAGRLD FGQVNELEGG KFFPATQSGL RDPDAPDDVA NGMPPRDGEI ASGGRTADAR AQLNEPDSVA HWQKHAVRSG QSLQISWSYS MPHKTRRWTY WITKPGWDTQ ARLARAHFEP DPLKVYLNTY QPYWGPDADK ELIPQGETIH EFNLPTRTGY HVLLAVWDVA DTANAFYQVI DLNFA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCACTACC GATTCGAGAA GCGCAACGAA GTCTTCGTCC ATTTCTCCGC GGCATGGCCG CGTCATCACG CCCGAGTCGC GCGCCGTGTA TCTCTACGAA GCAGGAAGGC TCGATTTCGG GCAGGTCAAT GAGCTGGAGG GCGGCAAATT CTTTCCCGCG ACCCAAAGCG GGCTCAGAGA TCCGGACGCG CCGGACGACG TCGCCAACGG CATGCCGCCG CGCGACGGCG AAATCGCGAG CGGCGGGCGC ACGGCGGATG CCCGCGCGCA ACTCAACGAA CCCGATTCCG TTGCGCATTG GCAGAAACAT GCGGTGCGAT CGGGGCAATC GCTGCAAATC TCGTGGTCGT ATTCGATGCC GCACAAGACG CGGCGCTGGA CTTACTGGAT CACGAAGCCG GGATGGGACA CCCAGGCGCG GCTTGCGCGC GCGCATTTCG AGCCCGATCC GCTGAAGGTC TACCTGAACA CGTATCAGCC GTACTGGGGG CCGGATGCGG ACAAGGAGTT GATCCCCCAG GGCGAGACGA TTCATGAATT CAACCTGCCG ACTCGCACCG GCTATCACGT ACTGCTCGCG GTGTGGGACG TCGCCGATAC CGCCAACGCG TTCTATCAGG TGATCGATCT GAATTTCGCG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA