BupsA.17449.a

YdjC-like family protein

CENTER ID: BupsA.17449.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17449.a.A1.GE31290 Full length( BupsA.17449.a ) 1 289
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2988
RefSeq: YP_334242.1
UniProt: Q3JQB1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAACDAAPRA LIFTADDFGL HERVNAAVER AHRDGVLSAA SLMVGAPAAR DAIERAKRLP SLAVGLHIVL ADGPATLPAH AIAALVGPDG RFRDAMAKDG CRFFFLPHVR AQLRREIRAQ FEAFASTGLV LDHVNTHKHF HLHPTVLSMI VDIGREFGLA AMRLPYEPST PFWLKPWIAL VRARLDRAGI AHNDYVVGIE HTGAMDEAAL LAALAKLPAG VGEIYSHPAE AGDGPITPSM RDYRPADELA ALLSPRVAAA VQAAGVACGG FADVFGGGAR ARISGAQPS
NT Sequence
GTGGCGGCTT GTGACGCGGC GCCGCGCGCG CTGATCTTCA CGGCGGACGA CTTCGGCCTG CATGAGCGGG TCAACGCGGC CGTCGAGCGT GCGCACCGCG ACGGCGTGCT GTCGGCCGCG AGCCTGATGG TCGGCGCGCC GGCCGCGCGC GACGCGATCG AGCGGGCGAA GCGCCTGCCA TCGCTCGCCG TCGGCCTGCA CATCGTGCTC GCCGACGGCC CCGCGACGCT GCCCGCGCAT GCGATTGCGG CGCTCGTCGG CCCCGACGGG CGCTTTCGCG ACGCGATGGC GAAGGACGGC TGCCGCTTCT TCTTCCTGCC GCACGTGCGG GCGCAACTGC GCCGCGAGAT CCGCGCGCAG TTCGAAGCGT TCGCGTCGAC CGGGCTCGTG CTCGATCACG TGAACACGCA CAAGCATTTC CATCTGCATC CGACCGTGCT GTCGATGATC GTCGACATCG GCCGCGAGTT CGGGCTTGCC GCGATGCGGC TGCCGTACGA GCCTTCGACG CCGTTCTGGC TGAAGCCGTG GATCGCGCTC GTGCGCGCGC GGCTCGACCG CGCGGGCATC GCGCACAACG ATTACGTCGT CGGCATCGAA CACACGGGCG CGATGGACGA GGCGGCGCTG CTCGCCGCGC TCGCGAAGCT GCCGGCGGGC GTCGGCGAGA TCTATTCCCA TCCGGCCGAG GCGGGCGACG GCCCGATCAC GCCTTCGATG CGCGACTACC GGCCGGCCGA TGAGCTTGCC GCGCTGCTGT CGCCGCGCGT CGCGGCCGCG GTGCAGGCGG CGGGCGTCGC GTGCGGCGGC TTCGCCGACG TGTTCGGCGG CGGCGCGCGT GCGCGGATCA GCGGAGCGCA GCCGTCA
Details for BupsA.17449.a.A1.GE31290
HARVESTED ON: 1/26/2011
SEQUENCED ON: 1/31/2011
EXPECTED MW: 32kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMVAACDA APRALIFTAD DFGLHERVNA AVERAHRDGV LSAASLMVGA PAARDAIERA KRLPSLAVGL HIVLADGPAT LPAHAIAALV GPDGRFRDAM AKDGCRFFFL PHVRAQLRRE IRAQFEAFAS TGLVLDHVNT HKHFHLHPTV LSMIVDIGRE FGLAAMRLPY EPSTPFWLKP WIALVRARLD RAGIAHNDYV VGIEHTGAMD EAALLAALAK LPAGVGEIYS HPAEAGDGPI TPSMRDYRPA DELAALLSPR VAAAVQAAGV ACGGFADVFG GGARARISGA QPS
Validated NT Sequence
ttattatgac ggctgcgctc cgctgatccg cgcacgcgcg ccgccgccga acacgtcggc gaagccgccg cacgcgacgc ccgccgcctg caccgcggcc gcgacgcgcg gcgacagcag cgcggcaagc tcatcggccg gccggtagtc gcgcatcgaa ggcgtgatcg ggccgtcgcc cgcctcggcc ggatgggaat agatctcgcc gacgcccgcc ggcagcttcg cgagcgcggc gagcagcgcc gcctcgtcca tcgcgcccgt gtgttcgatg ccgacgacgt aatcgttgtg cgcgatgccc gcgcggtcga gccgcgcgcg cacgagcgcg atccacggct tcagccagaa cggcgtcgaa ggctcgtacg gcagccgcat cgcggcaagc ccgaactcgc ggccgatgtc gacgatcatc gacagcacgg tcggatgcag atggaaatgc ttgtgcgtgt tcacgtgatc gagcacgagc ccggtcgacg cgaacgcttc gaactgcgcg cggatctcgc ggcgcagttg cgcccgcacg tgcggcagga agaagaagcg gcagccgtcc ttcgccatcg cgtcgcgaaa gcgcccgtcg gggccgacga gcgccgcaat cgcatgcgcg ggcagcgtcg cggggccgtc ggcgagcacg atgtgcaggc cgacggcgag cgatggcagg cgcttcgccc gctcgatcgc gtcgcgcgcg gccggcgcgc cgaccatcag gctcgcggcc gacagcacgc cgtcgcggtg cgcacgctcg acggccgcgt tgacccgctc atgcaggccg aagtcgtccg ccgtgaagat cagcgcgcgc ggcgccgcgt cacaagccgc caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtganggng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAACDAAP RALIFTADDF GLHERVNAAV ERAHRDGVLS AASLMVGAPA ARDAIERAKR LPSLAVGLHI VLADGPATLP AHAIAALVGP DGRFRDAMAK DGCRFFFLPH VRAQLRREIR AQFEAFASTG LVLDHVNTHK HFHLHPTVLS MIVDIGREFG LAAMRLPYEP STPFWLKPWI ALVRARLDRA GIAHNDYVVG IEHTGAMDEA ALLAALAKLP AGVGEIYSHP AEAGDGPITP SMRDYRPADE LAALLSPRVA AAVQAAGVAC GGFADVFGGG ARARISGAQP S
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcggct tgtgacgcgg cgccgcgcgc gctgatcttc acggcggacg acttcggcct gcatgagcgg gtcaacgcgg ccgtcgagcg tgcgcaccgc gacggcgtgc tgtcggccgc gagcctgatg gtcggcgcgc cggccgcgcg cgacgcgatc gagcgggcga agcgcctgcc atcgctcgcc gtcggcctgc acatcgtgct cgccgacggc cccgcgacgc tgcccgcgca tgcgattgcg gcgctcgtcg gccccgacgg gcgctttcgc gacgcgatgg cgaaggacgg ctgccgcttc ttcttcctgc cgcacgtgcg ggcgcaactg cgccgcgaga tccgcgcgca gttcgaagcg ttcgcgtcga ccgggctcgt gctcgatcac gtgaacacgc acaagcattt ccatctgcat ccgaccgtgc tgtcgatgat cgtcgacatc ggccgcgagt tcgggcttgc cgcgatgcgg ctgccgtacg agccttcgac gccgttctgg ctgaagccgt ggatcgcgct cgtgcgcgcg cggctcgacc gcgcgggcat cgcgcacaac gattacgtcg tcggcatcga acacacgggc gcgatggacg aggcggcgct gctcgccgcg ctcgcgaagc tgccggcggg cgtcggcgag atctattccc atccggccga ggcgggcgac ggcccgatca cgccttcgat gcgcgactac cggccggccg atgagcttgc cgcgctgctg tcgccgcgcg tcgcggccgc ggtgcaggcg gcgggcgtcg cgtgcggcgg cttcgccgac gtgttcggcg gcggcgcgcg tgcgcggatc agcggagcgc agccgtcata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a