BupsA.17399.a

Phenylacetate-CoA oxygenase/reductase, PaaK subunit

CENTER ID: BupsA.17399.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17399.a.A1.GE31256 Full length( BupsA.17399.a ) 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.7
RefSeq: YP_331429.1
UniProt: Q3JYC4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATPQFHPLR IRDVRPETAD AVTVSFEVPP ELRDAYRFTQ GQFVTLKAHV DGEETRRSYS ICVGTTDYDR DGELRIGIKR VRGGRFSNFA FDTLKPGHTI DVMTPDGRFF THLNAGHGKQ YVAFAGGSGI TPVLAIVKTT LELEPRSTFT LIYGNRSVDS IMFAEELEDL KNRFMQRLAL YHVLSDDVQD VELFNGVLDQ AKCAAFLDSL VPAATIDEAF ICGPAPMMDA AEAALAAAGV PRERVHVERF GTPLPQAGAP AVEITADTPA ADLEIVLDGK KRKLRLPYEG ASLLDVGLRA GLALPYACKG GVCCTCRAKV LEGEVRMEKN YTLEPQEIAD GFVLTCQCHP VSEKIVVSYD ER
NT Sequence
ATGGCGACTC CGCAATTCCA TCCGCTGCGC ATTCGCGACG TGCGGCCCGA AACCGCCGAC GCCGTCACGG TGTCGTTCGA AGTGCCGCCC GAGCTGCGCG ACGCTTACCG CTTCACGCAA GGCCAGTTCG TCACGCTGAA GGCGCACGTC GACGGCGAGG AAACCCGCCG CTCGTATTCG ATCTGCGTCG GCACGACGGA TTACGACCGC GACGGCGAGT TGCGCATCGG CATCAAGCGC GTGCGCGGCG GCCGCTTCTC GAACTTCGCG TTCGATACGC TCAAGCCCGG CCACACGATC GACGTGATGA CGCCGGACGG CCGCTTCTTC ACGCACCTGA ACGCCGGGCA CGGCAAGCAG TACGTCGCGT TCGCGGGCGG CTCGGGGATC ACGCCCGTGC TCGCGATCGT CAAGACGACG CTCGAGCTCG AGCCGCGCAG CACGTTCACG CTGATCTACG GCAATCGCAG CGTCGATTCG ATCATGTTCG CCGAGGAACT CGAGGACCTG AAGAACCGCT TCATGCAGCG GCTCGCCCTC TACCACGTGC TGTCCGACGA CGTGCAGGAC GTCGAGCTGT TCAACGGCGT GCTCGATCAG GCGAAATGCG CGGCGTTTCT CGATTCGCTC GTGCCGGCCG CGACGATCGA CGAGGCGTTC ATCTGCGGGC CGGCGCCGAT GATGGACGCC GCGGAAGCCG CGCTCGCCGC GGCCGGCGTG CCGCGCGAGC GCGTGCATGT CGAGCGCTTC GGCACGCCGC TGCCGCAGGC GGGCGCGCCC GCCGTCGAAA TCACCGCCGA CACGCCCGCC GCCGACCTCG AAATCGTGCT CGACGGCAAG AAGCGCAAGC TGCGGCTGCC GTACGAAGGC GCGAGCCTCC TTGATGTCGG GCTGCGCGCG GGCCTCGCGC TGCCGTACGC GTGCAAGGGC GGCGTCTGCT GCACGTGCCG CGCGAAGGTG CTCGAAGGCG AGGTGCGGAT GGAGAAGAAC TACACGCTGG AGCCGCAGGA GATCGCCGAC GGATTCGTGC TCACCTGCCA GTGCCATCCG GTCAGCGAGA AGATCGTCGT CAGCTACGAC GAACGG
Details for BupsA.17399.a.A1.GE31256
HARVESTED ON: 1/11/2011
SEQUENCED ON: 1/18/2011
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 87
Validated AA Sequence
MATPQFHPLR IRXXXPETAD AVTVSFEVPP ELRDAYRFTQ GQFVTLKAHV DGEETRRSYS ICVGTTDYDR DGELRIGIKR VRGGRFSNFA FDTLKPGHTI DVMTPDGRFF THLNAGHGKQ YVAFAGGSGI TPVLAIVKTT LELEPRSTFT LIYGNRSVDS IMFAEELEDL KNRFMQRLAL YHVLSDDVQD VELFNGVLDQ AKCAAFLDSL VPAATIDEAF ICGPAPMMDA AEAALAAAGV PRERVHVERF GTPLPQAGAP AVEITADTPA ADLEIVLDGK KRKLRLPYEG ASLLDVGLRA GLALPYACKG GVCCTCRAKV LEGEVRMEKN YTLEPQE
Validated NT Sequence
accctggaag ctcagaccca gggtcctggt tcgatggcga ctccgcaatt ccatccgctg cgcattcgcg nnnngnggcc cgaaaccgcc gacgccgtca cggtgtcgtt cgaagtgccg cccgagctgc gcgacgctta ccgcttcacg caaggccagt tcgtcacgct gaaggcgcac gtcgacggcg aggaaacccg ccgctcgtat tcgatctgcg tcggcacgac ggattacgac cgcgacggcg agttgcgcat cggcatcaag cgcgtgcgcg gcggccgctt ctcgaacttc gcgttcgata cgctcaagcc cggccacacg atcgacgtga tgacgccgga cggccgcttc ttcacgcacc tgaacgccgg gcacggcaag cagtacgtcg cgttcgcggg cggctcgggg atcacgcccg tgctcgcgat cgtcaagacg acgctcgagc tcgagccgcg cagcacgttc acgctgatct acggcaatcg cagcgtcgat tcgatcatgt tcgccgagga actcgaggac ctgaagaacc gcttcatgca gcggctcgcc ctctaccacg tgctgtccga cgacgtgcag gacgtcgagc tgttcaacgg cgtgctcgat caggcgaaat gcgcggcgtt tctcgattcg ctcgtgccgg ccgcgacgat cgacgaggcg ttcatctgcg ggccggcgcc gatgatggac gccgcggaag ccgcgctcgc cgcggccggc gtgccgcgcg agcgcgtgca tgtcgagcgc ttcggcacgc cgctgccgca ggcgggcgcg cccgccgtcg aaatcaccgc cgacacgccc gccgccgacc tcgaaatcgt gctcgacggc aagaagcgca agctgcggct gccgtacgaa ggcgcgagcc tccttgatgt cgggctgcgc gcgggcctcg cgctgccgta cgcgtgcaag ggcggcgtct gctgcacgtg ccgcgcgaag gtgctcgaag gcgaggtgcg gatggagaag aactacacgc tggagccgca ggagat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATPQFHPL RIRDVRPETA DAVTVSFEVP PELRDAYRFT QGQFVTLKAH VDGEETRRSY SICVGTTDYD RDGELRIGIK RVRGGRFSNF AFDTLKPGHT IDVMTPDGRF FTHLNAGHGK QYVAFAGGSG ITPVLAIVKT TLELEPRSTF TLIYGNRSVD SIMFAEELED LKNRFMQRLA LYHVLSDDVQ DVELFNGVLD QAKCAAFLDS LVPAATIDEA FICGPAPMMD AAEAALAAAG VPRERVHVER FGTPLPQAGA PAVEITADTP AADLEIVLDG KKRKLRLPYE GASLLDVGLR AGLALPYACK GGVCCTCRAK VLEGEVRMEK NYTLEPQEIA DGFVLTCQCH PVSEKIVVSY DER
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgactccg caattccatc cgctgcgcat tcgcgacgtg cggcccgaaa ccgccgacgc cgtcacggtg tcgttcgaag tgccgcccga gctgcgcgac gcttaccgct tcacgcaagg ccagttcgtc acgctgaagg cgcacgtcga cggcgaggaa acccgccgct cgtattcgat ctgcgtcggc acgacggatt acgaccgcga cggcgagttg cgcatcggca tcaagcgcgt gcgcggcggc cgcttctcga acttcgcgtt cgatacgctc aagcccggcc acacgatcga cgtgatgacg ccggacggcc gcttcttcac gcacctgaac gccgggcacg gcaagcagta cgtcgcgttc gcgggcggct cggggatcac gcccgtgctc gcgatcgtca agacgacgct cgagctcgag ccgcgcagca cgttcacgct gatctacggc aatcgcagcg tcgattcgat catgttcgcc gaggaactcg aggacctgaa gaaccgcttc atgcagcggc tcgccctcta ccacgtgctg tccgacgacg tgcaggacgt cgagctgttc aacggcgtgc tcgatcaggc gaaatgcgcg gcgtttctcg attcgctcgt gccggccgcg acgatcgacg aggcgttcat ctgcgggccg gcgccgatga tggacgccgc ggaagccgcg ctcgccgcgg ccggcgtgcc gcgcgagcgc gtgcatgtcg agcgcttcgg cacgccgctg ccgcaggcgg gcgcgcccgc cgtcgaaatc accgccgaca cgcccgccgc cgacctcgaa atcgtgctcg acggcaagaa gcgcaagctg cggctgccgt acgaaggcgc gagcctcctt gatgtcgggc tgcgcgcggg cctcgcgctg ccgtacgcgt gcaagggcgg cgtctgctgc acgtgccgcg cgaaggtgct cgaaggcgag gtgcggatgg agaagaacta cacgctggag ccgcaggaga tcgccgacgg attcgtgctc acctgccagt gccatccggt cagcgagaag atcgtcgtca gctacgacga acggtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa