BupsA.17377.a

LacI family regulatory protein

CENTER ID: BupsA.17377.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17377.a.A1.GE31246 Full length( BupsA.17377.a ) 1 384
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: WP_011326322.1
RefSeq: YP_336344.1
UniProt: Q3JJA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRASILKRVS IAQDSFSFRI CSGPNSNREQ SPVSRITSIQ VAQLAGVSRT TVSFVLNGIT GMGISEETRN RVLAAARKLG YVPNAMARSL ASGSTKTIAL LMPYGDHIHV DVDAYLPRFL AGLSAACHAT GYKVILEPVK ESHRPGAFLD LVNSGRIDGL VVLNPRKLDD GHFHELAHKG FPLVVFGSNL TETKSMCAIG IDNRSAAQRA TEHLLHLGHE RIAHIGFASD DYQVVRDRLD GFRLAMETRG LDVRPEWIAF ADYSAQSGYE AMRKMLAQPS RMTALFAGND TVAFGAMAAI RDVGLRVPDD LAVVGYDDIP LAAFATPPLT TVRTEPFKEG NDAAATLLGL IAGNPVDGLH SRGLAPLIVR QSCGASGPNI EKPN
NT Sequence
ATGCGCGCTT CTATACTAAA GCGCGTTAGT ATCGCGCAGG ATTCATTTAG CTTTCGCATT TGCTCTGGAC CTAATTCGAA TCGGGAGCAA TCTCCAGTGA GTCGCATAAC CAGCATCCAA GTAGCCCAAC TTGCCGGCGT GTCGAGAACA ACGGTGTCGT TTGTGTTGAA CGGCATCACC GGCATGGGAA TTAGCGAAGA AACTCGCAAT CGTGTGCTCG CGGCGGCCCG GAAGCTCGGT TATGTTCCGA ACGCCATGGC TCGGTCACTC GCCAGTGGCT CGACAAAGAC GATTGCTCTG CTCATGCCTT ACGGCGACCA CATCCATGTC GATGTCGATG CCTATCTCCC TCGGTTCCTC GCAGGATTGA GTGCCGCCTG TCATGCAACC GGTTACAAGG TGATACTCGA GCCTGTTAAG GAATCGCACC GCCCCGGGGC ATTCCTTGAT CTCGTGAATA GTGGGCGCAT TGACGGATTG GTGGTTTTAA ATCCTCGAAA GCTAGACGAT GGTCACTTTC ATGAGCTCGC TCACAAAGGC TTTCCGCTCG TTGTCTTCGG CTCGAACCTA ACCGAGACGA AATCGATGTG CGCCATTGGC ATCGACAATC GATCTGCGGC GCAACGGGCA ACGGAACATC TACTCCATCT CGGCCATGAG CGGATTGCTC ATATCGGCTT CGCGTCCGAT GACTATCAGG TAGTGCGCGA TCGACTGGAT GGCTTCCGGC TGGCAATGGA AACACGTGGC CTCGATGTAC GTCCTGAATG GATTGCATTT GCCGATTACA GCGCACAAAG CGGATATGAA GCCATGCGGA AAATGCTCGC CCAGCCTTCC CGCATGACAG CGCTGTTCGC CGGAAACGAC ACCGTCGCTT TTGGAGCAAT GGCCGCAATC CGCGATGTCG GATTGCGCGT TCCGGACGAC CTGGCGGTCG TCGGTTACGA CGACATTCCG CTCGCGGCAT TCGCGACGCC CCCCCTTACG ACGGTTCGCA CCGAACCCTT CAAGGAGGGA AATGACGCGG CGGCGACGTT GCTGGGGCTT ATCGCCGGCA ACCCGGTCGA CGGGCTTCAT TCCCGCGGAC TTGCGCCACT GATTGTTCGC CAATCCTGCG GCGCAAGCGG TCCAAATATC GAGAAACCAA AC
Details for BupsA.17377.a.A1.GE31246
HARVESTED ON: 1/11/2011
SEQUENCED ON: 1/18/2011
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MRASILKRVS IAQDSFSFRI CSGPNSNREQ SPVSRITSIQ VAQLAGVSRT TVSFVLNGIT GMGISEETRN RVLAAARKLG YVPNAMARSL ASGSTKTIAL LMPYGDHIHV DVDAYLPRFL AGLSAACHAT GYKVILEPVK ESHRPGAFLD LVNSGRIDGL VVLNPRKLDD GHFHELAHKG FPLVVFGSNL TETKSMCAIG IDNRSAAQRA TEHLLHLGHE RIAHIGFASD DYQVVRDRLD GFRLAMETRG LDVRPEWIAF ADYSAQSGYE AMRKMLAQPS RMTALFAGND TVAFGAMAAI RDVGLRVPDD LAVVGYDDIP LAAFATPPLT TVRTEPFKEG NDAAATLLGL IAGNPVDGLH SRGLAPLIVR QSCGASGPNI EKPN
Validated NT Sequence
gggtaccctg gaagctcaga cccagggtcc tggttcgatg cgcgcttcta tactaaagcg cgttagtatc gcgcaggatt catttagctt tcgcatttgc tctggaccta attcgaatcg ggagcaatct ccagtgagtc gcataaccag catccaagta gcccaacttg ccggcgtgtc gagaacaacg gtgtcgtttg tgttgaacgg catcaccggc atgggaatta gcgaagaaac tcgcaatcgt gtgctcgcgg cggcccggaa gctcggttat gttccgaacg ccatggctcg gtcactcgcc agtggctcga caaagacgat tgctctgctc atgccttacg gcgaccacat ccatgtcgat gtcgatgcct atctccctcg gttcctcgca ggattgagtg ccgcctgtca tgcaaccggt tacaaggtga tactcgagcc tgttaaggaa tcgcaccgcc ccggggcatt ccttgatctc gtgaatagtg ggcgcattga cggattggtg gttttaaatc ctcgaaagct agacgatggt cactttcatg agctcgctca caaaggcttt ccgctcgttg tcttcggctc gaacctaacc gagacgaaat cgatgtgcgc cattggcatc gacaatcgat ctgcggcgca acgggcaacg gaacatctac tccatctcgg ccatgagcgg attgctcata tcggcttcgc gtccgatgac tatcaggtag tgcgcgatcg actggatggc ttccggctgg caatggaaac acgtggcctc gatgtacgtc ctgaatggat tgcatttgcc gattacagcg cacaaagcgg atatgaagcc atgcggaaaa tgctcgccca gccttcccgc atgacagcgc tgttcgccgg aaacgacacc gtcgcttttg gagcaatggc cgcaatccgc gatgtcggat tgcgcgttcc ggacgacctg gcggtcgtcg gttacgacga cattccgctc gcggcattcg cgacgccccc ccttacgacg gttcgcaccg aacccttcaa ggagggaaat gacgcggcgg cgacgttgct ggggcttatc gccggcaacc cggtcgacgg gcttcattcc cgcggacttg cgccactgat tgttcgccaa tcctgcggcg caagcggtcc aaatatcgag aaaccaaact a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRASILKRV SIAQDSFSFR ICSGPNSNRE QSPVSRITSI QVAQLAGVSR TTVSFVLNGI TGMGISEETR NRVLAAARKL GYVPNAMARS LASGSTKTIA LLMPYGDHIH VDVDAYLPRF LAGLSAACHA TGYKVILEPV KESHRPGAFL DLVNSGRIDG LVVLNPRKLD DGHFHELAHK GFPLVVFGSN LTETKSMCAI GIDNRSAAQR ATEHLLHLGH ERIAHIGFAS DDYQVVRDRL DGFRLAMETR GLDVRPEWIA FADYSAQSGY EAMRKMLAQP SRMTALFAGN DTVAFGAMAA IRDVGLRVPD DLAVVGYDDI PLAAFATPPL TTVRTEPFKE GNDAAATLLG LIAGNPVDGL HSRGLAPLIV RQSCGASGPN IEKPN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgcttct atactaaagc gcgttagtat cgcgcaggat tcatttagct ttcgcatttg ctctggacct aattcgaatc gggagcaatc tccagtgagt cgcataacca gcatccaagt agcccaactt gccggcgtgt cgagaacaac ggtgtcgttt gtgttgaacg gcatcaccgg catgggaatt agcgaagaaa ctcgcaatcg tgtgctcgcg gcggcccgga agctcggtta tgttccgaac gccatggctc ggtcactcgc cagtggctcg acaaagacga ttgctctgct catgccttac ggcgaccaca tccatgtcga tgtcgatgcc tatctccctc ggttcctcgc aggattgagt gccgcctgtc atgcaaccgg ttacaaggtg atactcgagc ctgttaagga atcgcaccgc cccggggcat tccttgatct cgtgaatagt gggcgcattg acggattggt ggttttaaat cctcgaaagc tagacgatgg tcactttcat gagctcgctc acaaaggctt tccgctcgtt gtcttcggct cgaacctaac cgagacgaaa tcgatgtgcg ccattggcat cgacaatcga tctgcggcgc aacgggcaac ggaacatcta ctccatctcg gccatgagcg gattgctcat atcggcttcg cgtccgatga ctatcaggta gtgcgcgatc gactggatgg cttccggctg gcaatggaaa cacgtggcct cgatgtacgt cctgaatgga ttgcatttgc cgattacagc gcacaaagcg gatatgaagc catgcggaaa atgctcgccc agccttcccg catgacagcg ctgttcgccg gaaacgacac cgtcgctttt ggagcaatgg ccgcaatccg cgatgtcgga ttgcgcgttc cggacgacct ggcggtcgtc ggttacgacg acattccgct cgcggcattc gcgacgcccc cccttacgac ggttcgcacc gaacccttca aggagggaaa tgacgcggcg gcgacgttgc tggggcttat cgccggcaac ccggtcgacg ggcttcattc ccgcggactt gcgccactga ttgttcgcca atcctgcggc gcaagcggtc caaatatcga gaaaccaaac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa