SSGCID: Target Page

BupsA.17372.a

Transposase

CENTER ID:	BupsA.17372.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17372.a.A1.GE31244	Full length( BupsA.17372.a )		1	154

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
RefSeq:	YP_333564.1
UniProt:	Q3JS89

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAWGSPLNCR IQSSLKMASR HTTVFSTNNK QEIMEVLMGP ERRWRWSVKE KLATARESFE PGKSVSTVAR RHRVNPDQLL HWRKLYQDGS LSAVSAGEEV VPTSNLSNAL KQIRELQQLL GKKTVDNEIP REAPEAAKSQ ARIARLLSMP GDEQ

NT Sequence

ATGGCTTGGG GCAGCCCCCT GAATTGCCGA ATACAGTCCT CACTTAAAAT GGCGAGTAGG CATACGACGG TGTTTTCGAC TAACAACAAA CAAGAAATAA TGGAAGTGTT GATGGGACCG GAGCGACGAT GGCGATGGTC AGTGAAGGAG AAGCTGGCGA CGGCGCGAGA GAGCTTCGAG CCGGGCAAGT CGGTCTCAAC GGTTGCACGG CGGCACCGTG TCAATCCCGA CCAGTTGCTC CATTGGCGCA AGCTGTATCA GGATGGCAGT CTGTCGGCAG TCAGTGCCGG CGAAGAAGTT GTGCCAACGT CGAATCTGAG CAATGCCCTC AAGCAAATTC GTGAGCTTCA GCAACTGCTC GGCAAAAAGA CCGTAGATAA CGAAATTCCT CGTGAGGCAC CGGAGGCGGC GAAGTCGCAA GCAAGGATTG CGCGCTTGCT CTCCATGCCG GGGGACGAAC AG

Details for BupsA.17372.a.A1.GE31244

HARVESTED ON:	1/11/2011
SEQUENCED ON:	1/18/2011
EXPECTED MW:	19kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAWGSPLNC RIQSSLKMAS RHTTVFSTNN KQEIMEVLMG PERRWRWSVK EKLATARESF EPGKSVSTVA RRHRVNPDQL LHWRKLYQDG SLSAVSAGEE VVPTSNLSNA LKQIRELQQL LGKKTVDNEI PREAPEAAKS QARIARLLSM PGD

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctt ggggcagccc cctgaattgc cgaatacagt cctcacttaa aatggcgagt aggcatacga cggtgttttc gactaacaac aaacaagaaa taatggaagt gttgatggga ccggagcgac gatggcgatg gtcagtgaag gagaagctgg cgacggcgcg agagagcttc gagccgggca agtcggtctc aacggttgca cggcggcacc gtgtcaatcc cgaccagttg ctccattggc gcaagctgta tcaggatggc agtctgtcgg cagtcagtgc cggcgaagaa gttgtgccaa cgtcgaatct gagcaatgcc ctcaagcaaa ttcgtgagct tcagcaactg ctcggcaaaa agaccgtaga taacgaaatt cctcgtgagg caccggaggc ggcgaagtcg caagcaagga ttgcgcgctt gctctccatg ccgggggacg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAWGSPLNC RIQSSLKMAS RHTTVFSTNN KQEIMEVLMG PERRWRWSVK EKLATARESF EPGKSVSTVA RRHRVNPDQL LHWRKLYQDG SLSAVSAGEE VVPTSNLSNA LKQIRELQQL LGKKTVDNEI PREAPEAAKS QARIARLLSM PGDEQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcttggggc agccccctga attgccgaat acagtcctca cttaaaatgg cgagtaggca tacgacggtg ttttcgacta acaacaaaca agaaataatg gaagtgttga tgggaccgga gcgacgatgg cgatggtcag tgaaggagaa gctggcgacg gcgcgagaga gcttcgagcc gggcaagtcg gtctcaacgg ttgcacggcg gcaccgtgtc aatcccgacc agttgctcca ttggcgcaag ctgtatcagg atggcagtct gtcggcagtc agtgccggcg aagaagttgt gccaacgtcg aatctgagca atgccctcaa gcaaattcgt gagcttcagc aactgctcgg caaaaagacc gtagataacg aaattcctcg tgaggcaccg gaggcggcga agtcgcaagc aaggattgcg cgcttgctct ccatgccggg ggacgaacag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa