BupsA.17370.a

Branched-chain amino acid ABC transporter, periplasmic branched-chain amino acid-binding protein

CENTER ID: BupsA.17370.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17370.a.A2.GE31242 Mature protein( BupsA.17370.a ) 24 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.192
RefSeq: YP_331614.1
UniProt: Q3JXT9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLKTLAHAC LAVAAAWSVG AAQAADSVKI GFITDMSGLY ADIDGQGGLE AIKMAVADFG GKVNGKPIEV VYADHQNKAD IAASKAREWM DRGGLDLLVG GTNSATALSM NQVAAEKKKV YINIGAGADT LTNEQCTPYT VHYAYDTMAL AKGTGSAVVK QGGKTWFFLT ADYAFGKALE KNTADVVKAN GGKVLGEVRH PLSASDFSSF LLQAQSSKAQ ILGLANAGGD TVNAIKAAKE FGITKTMKLA ALLMFINDVH ALGLETTQGL VLTDSWYWNR DQASRQWAQR YFAKMKKMPS SLQAADYSSV TTYLKAVQAA GSTDSDKVMA QLKKMKIDDF YAKGSIRTDG SMIHDMYLME VKKPSESKEP WDYYKVVATI PGEQAFTTKQ ETRCALWK
NT Sequence
ATGAAATTGA AGACCCTTGC GCACGCCTGT CTTGCCGTCG CCGCCGCGTG GTCGGTGGGC GCCGCGCAGG CCGCGGATTC CGTGAAGATC GGCTTCATCA CCGACATGTC CGGCCTCTAT GCCGACATCG ACGGGCAGGG CGGCCTCGAG GCGATCAAGA TGGCGGTGGC CGACTTCGGC GGCAAGGTCA ACGGCAAGCC GATCGAGGTC GTGTATGCGG ACCACCAGAA CAAGGCGGAC ATCGCCGCAT CGAAGGCGCG CGAATGGATG GACCGCGGCG GGCTCGACCT GCTCGTCGGC GGCACGAACT CGGCGACCGC GCTGTCGATG AACCAGGTCG CGGCCGAGAA GAAGAAGGTC TACATCAACA TCGGCGCGGG CGCGGACACG CTGACGAACG AGCAGTGCAC GCCGTACACG GTCCACTACG CGTACGACAC GATGGCGCTC GCGAAGGGCA CGGGCTCGGC GGTGGTGAAG CAGGGCGGCA AGACGTGGTT CTTCCTGACC GCCGATTACG CGTTCGGCAA GGCGCTCGAG AAGAACACCG CGGACGTCGT CAAGGCCAAC GGCGGCAAGG TGCTCGGCGA AGTGCGCCAT CCGCTGTCGG CGTCGGATTT CTCGTCGTTC CTGTTGCAGG CGCAGTCGTC GAAGGCGCAG ATCCTCGGCC TCGCGAACGC GGGGGGCGAC ACGGTGAACG CGATCAAGGC GGCGAAGGAA TTCGGCATCA CGAAGACGAT GAAGCTCGCC GCGCTGCTGA TGTTCATCAA CGATGTCCAC GCGCTCGGCC TCGAGACGAC GCAAGGTCTC GTGCTGACGG ACAGTTGGTA CTGGAACCGC GATCAGGCGT CGCGGCAGTG GGCGCAGCGC TATTTCGCGA AGATGAAGAA GATGCCGTCG AGCCTGCAGG CGGCGGACTA TTCGTCGGTG ACGACTTACC TGAAGGCGGT GCAGGCGGCG GGCTCGACCG ATTCCGACAA GGTGATGGCG CAGCTCAAGA AGATGAAGAT CGACGACTTC TATGCGAAGG GCTCCATCCG CACGGACGGC AGCATGATTC ACGACATGTA TCTGATGGAA GTGAAGAAGC CGTCCGAATC GAAGGAGCCG TGGGACTACT ACAAGGTCGT CGCGACGATT CCGGGCGAGC AGGCATTCAC GACGAAGCAG GAGACGCGCT GCGCGCTCTG GAAG
Details for BupsA.17370.a.A2.GE31242
HARVESTED ON: 1/11/2011
SEQUENCED ON: 1/18/2011
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MAADSVKIGF ITDMSGLYAD IDGQGGLEAI KMAVADFGGK VNGKPIEVVY ADHQNKADIA ASKAREWMDR GGLDLLVGGT NSATALSMNQ VAAEKKKVYI NIGAGADTLT NEQCTPYTVH YAYDTMALAK GTGSAVVKQG GKTWFFLTAD YAFGKALEKN TADVVKANGG KVLGEVRHPL SASDFSSFLL QAQSSKAQIL GLANAGGDTV NAIKAAKEFG ITKTMKLAAL LMFINDVHAL GLETTQGLVL TDSWYWNRDQ ASRQWAQRYF AKMKKMPSSL QAADYSSVTT YLKAVQAAGS TDSDKVMAQL KKMKIDDFYA KGSIRTDGSM IHDMYLMEVK KPSESKEPWD YYKVVATIPG EQAFTTKQET RCALWK
Validated NT Sequence
ttattacttc cagagcgcgc agcgcgtctc ctgcttcgtc gtgaatgcct gctcgcccgg aatcgtcgcg acgaccttgt agtagtccca cggctccttc gattcggacg gcttcttcac ttccatcaga tacatgtcgt gaatcatgct gccgtccgtg cggatggagc ccttcgcata gaagtcgtcg atcttcatct tcttgagctg cgccatcacc ttgtcggaat cggtcgagcc cgccgcctgc accgccttca ggtaagtcgt caccgacgaa tagtccgccg cctgcaggct cgacggcatc ttcttcatct tcgcgaaata ncgctgcgcc cactgccgcg acgcctgatc gcggttccag taccaactgt ccgtcagcac gagaccttgc gtcgtctcga ggccgagcgc gtggacatcg ttgatgaaca tcagcagcgc ggcgagcttc atcgtcttcg tgatgccgaa ttccttcgcc gccttgatcg cgttcaccgt gtcgcccccc gcgttcgcga ggccgaggat ctgcgccttc gacgactgcg cctgcaacag gaacgacgag aaatcngacg ccgacagcgg atggcgcact tcgccgagca ccttgccgcc gttggccttg acgacgtccg cggtgttctt ctcgagcgcc ttgccgaacg cgtaatcggc ggtcaggaag aaccacgtct tgccgccctg cttcaccacc gccgagcccg tgcccttcgc gagcgccatc gtgtcgtacg cgtagtggac cgtgtacggc gtgcactgct cgttcgtcag cgtgtccgcg cccgcgccga tgttgatgta gaccttcttc ttctcggccg cgacctggtt catcgacagc gcggtcgccg agttcgtgcc gccgacgagc aggtcgagcc cgccgcggtc catccattcg cgcgccttcg atgcggcgat gtccgccttg ttctggtggt ccgcatacac gacctcgatc ggcttgccgt tgaccttgcc gccgaagtcg gccaccgcca tcttgatcgc ctcgaggccg ccctgcccgt cgatgtcggc atagaggccg gacatgtcgg tgatgaagcc gatcttcacg gaatccgcgg ccatcgaacc aggaccctgg gtctgagctt ccagggtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAADSVKIG FITDMSGLYA DIDGQGGLEA IKMAVADFGG KVNGKPIEVV YADHQNKADI AASKAREWMD RGGLDLLVGG TNSATALSMN QVAAEKKKVY INIGAGADTL TNEQCTPYTV HYAYDTMALA KGTGSAVVKQ GGKTWFFLTA DYAFGKALEK NTADVVKANG GKVLGEVRHP LSASDFSSFL LQAQSSKAQI LGLANAGGDT VNAIKAAKEF GITKTMKLAA LLMFINDVHA LGLETTQGLV LTDSWYWNRD QASRQWAQRY FAKMKKMPSS LQAADYSSVT TYLKAVQAAG STDSDKVMAQ LKKMKIDDFY AKGSIRTDGS MIHDMYLMEV KKPSESKEPW DYYKVVATIP GEQAFTTKQE TRCALWK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgcggat tccgtgaaga tcggcttcat caccgacatg tccggcctct atgccgacat cgacgggcag ggcggcctcg aggcgatcaa gatggcggtg gccgacttcg gcggcaaggt caacggcaag ccgatcgagg tcgtgtatgc ggaccaccag aacaaggcgg acatcgccgc atcgaaggcg cgcgaatgga tggaccgcgg cgggctcgac ctgctcgtcg gcggcacgaa ctcggcgacc gcgctgtcga tgaaccaggt cgcggccgag aagaagaagg tctacatcaa catcggcgcg ggcgcggaca cgctgacgaa cgagcagtgc acgccgtaca cggtccacta cgcgtacgac acgatggcgc tcgcgaaggg cacgggctcg gcggtggtga agcagggcgg caagacgtgg ttcttcctga ccgccgatta cgcgttcggc aaggcgctcg agaagaacac cgcggacgtc gtcaaggcca acggcggcaa ggtgctcggc gaagtgcgcc atccgctgtc ggcgtcggat ttctcgtcgt tcctgttgca ggcgcagtcg tcgaaggcgc agatcctcgg cctcgcgaac gcggggggcg acacggtgaa cgcgatcaag gcggcgaagg aattcggcat cacgaagacg atgaagctcg ccgcgctgct gatgttcatc aacgatgtcc acgcgctcgg cctcgagacg acgcaaggtc tcgtgctgac ggacagttgg tactggaacc gcgatcaggc gtcgcggcag tgggcgcagc gctatttcgc gaagatgaag aagatgccgt cgagcctgca ggcggcggac tattcgtcgg tgacgactta cctgaaggcg gtgcaggcgg cgggctcgac cgattccgac aaggtgatgg cgcagctcaa gaagatgaag atcgacgact tctatgcgaa gggctccatc cgcacggacg gcagcatgat tcacgacatg tatctgatgg aagtgaagaa gccgtccgaa tcgaaggagc cgtgggacta ctacaaggtc gtcgcgacga ttccgggcga gcaggcattc acgacgaagc aggagacgcg ctgcgcgctc tggaagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa