SSGCID: Target Page

BupsA.17362.a

Flagellar rod assembly protein/muramidase FlgJ

CENTER ID:	BupsA.17362.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17362.a.A1.GE31235	Full length( BupsA.17362.a )		1	311

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.471
RefSeq:	YP_331885.1
UniProt:	Q3JX18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTFTKPNDL SQRFALDVQG FDALRSKATA AAPREGVKMV AGQFDAMFTQ MMLKSMRDAT PSDGLLDSSS SKMYTSMLDQ QLAQQMSSKG IGVADALTKQ LLRNANVAPD AQGEGGLAAM NALAKAYANS NGAPGNGALA GTRGYSAASA LTPPLKGNGN SAQADAFVEK MALAAQAASA TTGIPARFIV GQAALESGWG KREIRGANGE SSYNVFGIKA TKGWTGRTVS AVTTEYVNGK PHRVVAQFRA YDSYEHAMTD YANLLKNNPR YASVLNAGHN AEGFAHGMQK AGYATDPHYA KKLISIMQQI G

NT Sequence

GTGACGACTT TCACGAAACC GAACGATCTG TCGCAGCGCT TCGCGCTCGA CGTTCAGGGG TTCGACGCGC TGCGCTCGAA GGCCACGGCG GCTGCGCCGC GCGAGGGTGT GAAGATGGTC GCGGGCCAGT TCGACGCGAT GTTCACGCAG ATGATGCTGA AGAGCATGCG CGACGCGACG CCTTCGGACG GCCTGCTCGA TTCGAGCTCG TCGAAGATGT ACACGTCGAT GCTCGATCAG CAGCTCGCGC AGCAGATGTC GTCGAAGGGC ATCGGCGTGG CCGACGCGCT CACCAAGCAG TTGCTGCGCA ACGCGAACGT CGCGCCCGAC GCGCAGGGCG AAGGCGGCCT CGCGGCGATG AACGCGCTCG CGAAGGCGTA TGCGAACTCG AATGGGGCGC CAGGCAACGG CGCGCTCGCG GGCACGCGCG GCTATTCGGC GGCGAGCGCG CTCACGCCGC CGCTGAAGGG CAACGGCAAC TCCGCGCAGG CGGATGCGTT CGTCGAGAAG ATGGCGCTCG CCGCGCAGGC GGCGAGCGCG ACCACCGGCA TTCCGGCGCG CTTCATCGTC GGCCAGGCGG CGCTCGAATC CGGCTGGGGC AAGCGCGAGA TCCGCGGCGC GAACGGCGAA TCGAGCTACA ACGTGTTCGG CATCAAGGCG ACGAAGGGCT GGACCGGCCG CACGGTATCG GCCGTCACCA CCGAATACGT GAACGGCAAG CCGCACCGCG TGGTCGCGCA GTTCCGCGCA TACGACTCGT ACGAGCATGC GATGACCGAT TACGCGAACC TGCTGAAAAA CAATCCGCGC TACGCGAGCG TGCTGAACGC GGGCCACAAC GCGGAAGGCT TCGCGCATGG AATGCAGAAG GCCGGCTACG CAACCGATCC GCACTACGCG AAGAAGCTGA TTTCGATCAT GCAGCAGATC GGC

Details for BupsA.17362.a.A1.GE31235

HARVESTED ON:	1/11/2011
SEQUENCED ON:	1/18/2011
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMVTTFTK PNDLSQRFAL DVQGFDALRS KATAAAPREG VKMVAGQFDA MFTQMMLKSM RDATPSDGLL DSSSSKMYTS MLDQQLAQQM SSKGIGVADA LTKQLLRNAN VAPDAQGEGG LAAMNALAKA YANSNGAPGN GALAGTRGYS AASALTPPLK GNGNSAQADA FVEKMALAAQ AASATTGIPA RFIVGQAALE SGWGKREIRG ANGESSYNVF GIKATKGWTG RTVSAVTTEY VNGKPHRVVA QFRAYDSYEH AMTDYANLLK NNPRYASVLN AGHNAEGFAH GMQKAGYATD PHYAKKLISI MQQIG

Validated NT Sequence

ttattagccg atctgctgca tgatcgaaat cagcttcttc gcgtagtgcg gatcggttgc gtagccggcc ttctgcattc catgcgcgaa gccttccgcg ttgtggcccg cgttcagcac gctcgcgtag cgcggattgt ttttcagcag gttcgcgtaa tcggtcatcg catgctcgta cgagtcgtat gcgcggaact gcgcgaccac gcggtgcggc ttgccgttca cgtattcggt ggtgacggcc gataccgtgc ggccggtcca gcccttcgtc gccttgatgc cgaacacgtt gtagctcgat tcgccgttcg cgccgcggat ctcgcgcttg ccccagccgg attcgagcgc cgcctggccg acgatgaagc gcgccggaat gccggtggtc gcgctcgccg cctgcgcggc gagcgccatc ttctcgacga acgcatccgc ctgcgcggag ttgccgttgc ccttcagcgg cggcgtgagc gcgctcgccg ccgaatagcc gcgcgtgccc gcgagcgcgc cgttgcctgg cgccccattc gagttcgcat acgccttcgc gagcgcgttc atcgccgcga ggccgccttc gccctgcgcg tcgggcgcga cgttcgcgtt gcgcagcaac tgcttggtga gcgcgtcggc cacgccgatg cccttcgacg acatctgctg cgcgagctgc tgatcgagca tcgacgtgta catcttcgac gagctcgaat cgagcaggcc gtccgaaggc gtcgcgtcgc gcatgctctt cagcatcatc tgcgtgaaca tcgcgtcgaa ctggcccgcg accatcttca caccctcgcg cggcgcagcc gccgtggcct tcgagcgcag cgcgtcgaac ccctgaacgt cgagcgcgaa gcgctgcgac agatcgttcg gtttcgtgaa agtcgtcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga g

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTTFTKPN DLSQRFALDV QGFDALRSKA TAAAPREGVK MVAGQFDAMF TQMMLKSMRD ATPSDGLLDS SSSKMYTSML DQQLAQQMSS KGIGVADALT KQLLRNANVA PDAQGEGGLA AMNALAKAYA NSNGAPGNGA LAGTRGYSAA SALTPPLKGN GNSAQADAFV EKMALAAQAA SATTGIPARF IVGQAALESG WGKREIRGAN GESSYNVFGI KATKGWTGRT VSAVTTEYVN GKPHRVVAQF RAYDSYEHAM TDYANLLKNN PRYASVLNAG HNAEGFAHGM QKAGYATDPH YAKKLISIMQ QIG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacgact ttcacgaaac cgaacgatct gtcgcagcgc ttcgcgctcg acgttcaggg gttcgacgcg ctgcgctcga aggccacggc ggctgcgccg cgcgagggtg tgaagatggt cgcgggccag ttcgacgcga tgttcacgca gatgatgctg aagagcatgc gcgacgcgac gccttcggac ggcctgctcg attcgagctc gtcgaagatg tacacgtcga tgctcgatca gcagctcgcg cagcagatgt cgtcgaaggg catcggcgtg gccgacgcgc tcaccaagca gttgctgcgc aacgcgaacg tcgcgcccga cgcgcagggc gaaggcggcc tcgcggcgat gaacgcgctc gcgaaggcgt atgcgaactc gaatggggcg ccaggcaacg gcgcgctcgc gggcacgcgc ggctattcgg cggcgagcgc gctcacgccg ccgctgaagg gcaacggcaa ctccgcgcag gcggatgcgt tcgtcgagaa gatggcgctc gccgcgcagg cggcgagcgc gaccaccggc attccggcgc gcttcatcgt cggccaggcg gcgctcgaat ccggctgggg caagcgcgag atccgcggcg cgaacggcga atcgagctac aacgtgttcg gcatcaaggc gacgaagggc tggaccggcc gcacggtatc ggccgtcacc accgaatacg tgaacggcaa gccgcaccgc gtggtcgcgc agttccgcgc atacgactcg tacgagcatg cgatgaccga ttacgcgaac ctgctgaaaa acaatccgcg ctacgcgagc gtgctgaacg cgggccacaa cgcggaaggc ttcgcgcatg gaatgcagaa ggccggctac gcaaccgatc cgcactacgc gaagaagctg atttcgatca tgcagcagat cggctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa