BupsA.17360.a

AotO

CENTER ID: BupsA.17360.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17360.a.A1.GE31233 Full length( BupsA.17360.a ) 1 426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.5949
RefSeq: YP_336961.1
UniProt: Q3JHJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILTRRARRF RRKSLIGREN DYRTAQVWCV SRRSTYNRSF TDIERRAAAA PPPPIMQTQT HPLISPAVGT ARHITSFHYG PRGGKKVYIQ ASLHADELPG MLVATLLRRK LAALEAAGRL RDEIVVVPVA NPIGLAQHVF GDHLGRFELG SMQNFNRNFH DLAALVIPRI EGRLTHDAAA NLAAVRGAMR EALAEQKPRT ELESQRLALQ RLSYDADIVL DLHCDCDAVM HIYTNPDLWD DVEPLSRYLG AKASLLALNS VGNPFDEIHS FCWSELRGRF GERHPIPNGT ISVTVELRSE RDVSYELAEH DAQALVEYLT LRGAIDGTPA PQPPLEFAAT PLAGTDPLVA PVSGVIVFHT PVGVWIEAGQ DVADIVDPLT DRVVTLKSSV SGVLYARQIA RFATAGMEVA RIAGATPIRT GSLLSA
NT Sequence
ATGATTTTGA CTCGGCGGGC ACGCCGATTT CGACGCAAGT CATTGATCGG TCGAGAAAAC GACTATCGAA CGGCACAGGT TTGGTGCGTT TCGCGACGAT CCACGTACAA TCGTTCATTC ACCGACATCG AACGACGCGC GGCGGCTGCG CCGCCGCCTC CCATCATGCA AACGCAGACC CATCCGCTGA TCTCGCCGGC CGTCGGCACG GCGCGCCACA TCACGAGTTT CCATTACGGC CCGCGCGGCG GGAAGAAGGT GTACATCCAG GCGTCGCTGC ACGCGGACGA GCTGCCCGGC ATGCTCGTCG CCACGCTGCT GCGCCGCAAG CTCGCGGCGC TCGAGGCGGC GGGCAGGCTG CGCGACGAGA TCGTCGTCGT GCCGGTCGCG AACCCGATCG GCCTCGCGCA GCACGTGTTC GGCGATCATC TCGGCCGCTT CGAGCTCGGC TCGATGCAGA ACTTCAACCG CAATTTCCAC GATCTCGCCG CGCTCGTGAT TCCGCGCATC GAAGGGCGCC TCACGCACGA CGCGGCCGCG AACCTCGCCG CCGTGCGCGG CGCGATGCGC GAGGCGCTTG CCGAGCAGAA GCCGCGCACC GAGCTCGAAT CGCAGCGGCT CGCGCTGCAG CGGCTGTCGT ATGACGCGGA CATCGTGCTC GATCTGCACT GCGACTGCGA CGCGGTGATG CACATCTACA CGAATCCGGA CCTGTGGGAC GACGTCGAGC CGCTGTCGCG CTATCTGGGC GCGAAGGCGT CGCTGCTCGC GCTGAACTCG GTCGGCAATC CGTTCGACGA AATCCACAGC TTCTGCTGGT CCGAGCTGCG CGGCCGCTTC GGCGAACGTC ATCCGATTCC GAACGGCACG ATCTCGGTGA CGGTCGAGCT GCGCAGCGAG CGCGACGTGT CGTACGAGCT CGCCGAGCAC GACGCGCAGG CGCTCGTCGA ATACCTGACG CTGCGCGGCG CGATCGACGG CACGCCCGCG CCGCAGCCGC CGCTCGAATT CGCGGCCACG CCGCTCGCGG GCACCGATCC GCTCGTCGCG CCGGTGTCGG GCGTGATCGT GTTCCACACG CCGGTCGGCG TATGGATCGA GGCGGGCCAG GACGTGGCCG ACATCGTCGA TCCGCTGACC GATCGCGTCG TCACGTTGAA GAGCAGCGTG TCCGGCGTGC TGTATGCGCG GCAGATCGCG CGCTTCGCGA CGGCCGGCAT GGAAGTCGCG CGGATCGCCG GCGCGACGCC GATCCGCACC GGATCGCTGC TGTCGGCT
Details for BupsA.17360.a.A1.GE31233
HARVESTED ON: 1/11/2011
SEQUENCED ON: 1/18/2011
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MILTRRARRF RRKSLIGREN DYRTAQVWCV SRRSTYNRSF TDIERRAAAA PPPPIMQTQT HPLISPAVGT ARHITSFHYG PRGGKKVYIQ ASLHADELPG MLVATLLRRK LAALEAAGRL RDEIVVVPVA NPIGLAQHVF GDHLGRFELG SMQNFNRNFH DLAALVIPRI EGRLTHDAXA NLAAVRGAMR EALAEQKPRT ELESQRLALQ RLSYDADIVL DXHCDCDAVM HIYTNPDLWD DVEPLSRYLG AKASLLALNS VGNPFDEIHS FCWSELRGRF GERHPIPNGT ISVTVELRSE RDVSYELAEH DAQALVEYLT LRGAIDGTPA PQPPLEFAAT PLAGTDPLVA PVSGVIVFHT PVGVWIEAGQ DVADIVDPLT DRVVTLKSSV SGVLYARQIA RFATAGMEVA RIAGATPIRT GSLLSA
Validated NT Sequence
ggtaccctgg aagctcagac ccagggtcct ggttcgatga ttttgactcg gcgggcacgc cgatttcgac gcaagtcatt gatcggtcga gaaaacgact atcgaacggc acaggtttgg tgcgtttcgc gacgatccac gtacaatcgt tcattcaccg acatcgaacg acgcgcggcg gctgcgccgc cgcctcccat catgcaaacg cagacccatc cgctgatctc gccggccgtc ggcacggcgc gccacatcac gagtttccat tacggcccgc gcggcgggaa gaaggtgtac atccaggcgt cgctgcacgc ggacgagctg cccggcatgc tcgtcgccac gctgctgcgc cgcaagctcg cggcgctcga ggcggcgggc aggctgcgcg acgagatcgt cgtcgtgccg gtcgcgaacc cgatcggcct cgcgcagcac gtgttcggcg atcatctcgg ccgcttcgag ctcggctcga tgcagaactt caaccgcaat ttccacgatc tcgccgcgct cgtgattccg cgcatcgaag ggcgcctcac gcacgacgcg nccgcgaacc tcgccgccgt gcgcggcgcg atgcgcgagg cgcttgccga gcagaagccg cgcaccgagc tcgaatcgca gcggctcgcg ctgcagcggc tgtcgtatga cgcggacatc gtgctcgatn tgcactgcga ctgcgacgcg gtgatgcaca tctacacgaa tccggacctg tgggacgacg tcgagccgct gtcgcgctat ctgggcgcga aggcgtcgct gctcgcgctg aactcggtcg gcaatccgtt cgacgaaatc cacagcttct gctggtccga gctgcgcggc cgcttcggcg aacgtcatcc gattccgaac ggcacgatct cggtgacggt cgagctgcgc agcgagcgcg acgtgtcgta cgagctcgcc gagcacgacg cgcaggcgct cgtcgaatac ctgacgctgc gcggcgcgat cgacggcacg cccgcgccgc agccgccgct cgaattcgcg gccacgccgc tcgcgggcac cgatccgctc gtcgcgccgg tgtcgggcgt gatcgtgttc cacacgccgg tcggcgtatg gatcgaggcg ggccaggacg tggccgacat cgtcgatccg ctgaccgatc gcgtcgtcac gttgaagagc agcgtgtccg gcgtgctgta tgcgcggcag atcgcgcgct tcgcgacggc cggcatggaa gtcgcgcgga tcgccggcgc gacgccgatc cgcaccggat cgctgctgtc ggctt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILTRRARR FRRKSLIGRE NDYRTAQVWC VSRRSTYNRS FTDIERRAAA APPPPIMQTQ THPLISPAVG TARHITSFHY GPRGGKKVYI QASLHADELP GMLVATLLRR KLAALEAAGR LRDEIVVVPV ANPIGLAQHV FGDHLGRFEL GSMQNFNRNF HDLAALVIPR IEGRLTHDAA ANLAAVRGAM REALAEQKPR TELESQRLAL QRLSYDADIV LDLHCDCDAV MHIYTNPDLW DDVEPLSRYL GAKASLLALN SVGNPFDEIH SFCWSELRGR FGERHPIPNG TISVTVELRS ERDVSYELAE HDAQALVEYL TLRGAIDGTP APQPPLEFAA TPLAGTDPLV APVSGVIVFH TPVGVWIEAG QDVADIVDPL TDRVVTLKSS VSGVLYARQI ARFATAGMEV ARIAGATPIR TGSLLSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattttgact cggcgggcac gccgatttcg acgcaagtca ttgatcggtc gagaaaacga ctatcgaacg gcacaggttt ggtgcgtttc gcgacgatcc acgtacaatc gttcattcac cgacatcgaa cgacgcgcgg cggctgcgcc gccgcctccc atcatgcaaa cgcagaccca tccgctgatc tcgccggccg tcggcacggc gcgccacatc acgagtttcc attacggccc gcgcggcggg aagaaggtgt acatccaggc gtcgctgcac gcggacgagc tgcccggcat gctcgtcgcc acgctgctgc gccgcaagct cgcggcgctc gaggcggcgg gcaggctgcg cgacgagatc gtcgtcgtgc cggtcgcgaa cccgatcggc ctcgcgcagc acgtgttcgg cgatcatctc ggccgcttcg agctcggctc gatgcagaac ttcaaccgca atttccacga tctcgccgcg ctcgtgattc cgcgcatcga agggcgcctc acgcacgacg cggccgcgaa cctcgccgcc gtgcgcggcg cgatgcgcga ggcgcttgcc gagcagaagc cgcgcaccga gctcgaatcg cagcggctcg cgctgcagcg gctgtcgtat gacgcggaca tcgtgctcga tctgcactgc gactgcgacg cggtgatgca catctacacg aatccggacc tgtgggacga cgtcgagccg ctgtcgcgct atctgggcgc gaaggcgtcg ctgctcgcgc tgaactcggt cggcaatccg ttcgacgaaa tccacagctt ctgctggtcc gagctgcgcg gccgcttcgg cgaacgtcat ccgattccga acggcacgat ctcggtgacg gtcgagctgc gcagcgagcg cgacgtgtcg tacgagctcg ccgagcacga cgcgcaggcg ctcgtcgaat acctgacgct gcgcggcgcg atcgacggca cgcccgcgcc gcagccgccg ctcgaattcg cggccacgcc gctcgcgggc accgatccgc tcgtcgcgcc ggtgtcgggc gtgatcgtgt tccacacgcc ggtcggcgta tggatcgagg cgggccagga cgtggccgac atcgtcgatc cgctgaccga tcgcgtcgtc acgttgaaga gcagcgtgtc cggcgtgctg tatgcgcggc agatcgcgcg cttcgcgacg gccggcatgg aagtcgcgcg gatcgccggc gcgacgccga tccgcaccgg atcgctgctg tcggcttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa