BupsA.17357.a
Polysaccharide deacetylase family protein
| CENTER ID: | BupsA.17357.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.17357.a.A1.GE31231 | Full length( BupsA.17357.a ) | 1 | 317 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.17357.a.A1.PS01018 | Full length( BupsA.17357.a ) | 1 | 317 |
Structures
3S6O
DEPOSITED: 5/25/2011
DETERMINATION: XRay
CLONE: BupsA.17357.a.A1.GE31231
PROTEIN: BupsA.17357.a.A1.PS01018
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_146602 |
| PATRIC ID: | fig|320372.6.peg.2655 |
| RefSeq: | YP_333925.1 |
| UniProt: | Q3JR78 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAFDPNYPRD LIGYGRHPVQ ANWPGRARVA VQFVLNYEEG GENCVLHGDP ASEQFLSEIV GAAAYPARHM SMESIYEYGS RAGVWRILRE FDKRGLPLTV FGVGMAIERH PELARAFVEL GHEIACHGWR WIHYQDMTPE REAEHMRLGM EAIERVTGVR PLGWYTGRDS PNTHRLVAEY GGFLYDSDHY GDDLPFWMDV EVSGGASVPQ LIVPYTLDAN DMRFATPQGF NTADHFFHYL RDAFDVLYEE GDEAPKMMSI GMHCRLLGRP GRFRALQRFL DHIERHDRVW VARRVEIARH WREHHPYRPH ARREGDA
NT Sequence
ATGGCATTCG ATCCCAACTA TCCACGCGAT CTGATCGGCT ACGGCCGACA TCCGGTGCAG GCGAACTGGC CGGGCCGCGC GCGCGTCGCG GTGCAGTTCG TGCTCAACTA CGAGGAAGGC GGCGAGAACT GCGTGCTGCA CGGCGACCCC GCCTCCGAGC AGTTCCTGTC CGAGATCGTC GGCGCGGCCG CGTATCCGGC GCGCCACATG AGCATGGAGT CGATCTACGA ATACGGCTCG CGCGCGGGCG TGTGGCGCAT CCTGCGCGAG TTCGACAAGC GCGGGCTGCC GCTCACGGTG TTCGGCGTCG GCATGGCGAT CGAGCGGCAT CCGGAGCTCG CGCGCGCGTT CGTCGAGCTC GGCCACGAGA TCGCGTGTCA CGGCTGGCGC TGGATTCACT ATCAGGACAT GACGCCCGAG CGCGAAGCCG AGCACATGCG GCTCGGAATG GAGGCGATCG AGCGCGTGAC GGGCGTGCGC CCGCTCGGCT GGTATACGGG CCGCGACAGT CCGAACACGC ATCGGCTCGT CGCCGAGTAC GGCGGCTTCC TGTACGACTC CGATCATTAC GGCGACGATC TGCCGTTCTG GATGGACGTC GAGGTGTCGG GCGGCGCGAG CGTGCCGCAA CTGATCGTCC CGTACACGCT CGACGCGAAC GACATGCGTT TCGCGACGCC GCAGGGCTTC AACACCGCCG ATCATTTCTT CCACTATCTG CGCGACGCGT TCGACGTGCT GTACGAGGAA GGCGACGAAG CGCCCAAGAT GATGTCGATC GGCATGCACT GCCGCCTGCT CGGCCGGCCG GGGCGCTTTC GCGCGCTGCA GCGCTTTCTC GATCACATCG AGCGGCACGA TCGCGTGTGG GTCGCGCGCC GCGTCGAGAT CGCGCGGCAC TGGCGCGAGC ATCATCCGTA TCGGCCGCAC GCCCGGCGCG AGGGGGACGC A
Details for BupsA.17357.a.A1.GE31231
| HARVESTED ON: | 1/11/2011 |
| SEQUENCED ON: | 1/18/2011 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAFDPNYPR DLIGYGRHPV QANWPGRARV AVQFVLNYEE GGENCVLHGD PASEQFLSEI VGAAAYPARH MSMESIYEYG SRAGVWRILR EFDKRGLPLT VFGVGMAIER HPELARAFVE LGHEIACHGW RWIHYQDMTP EREAEHMRLG MEAIERVTGV RPLGWYTGRD SPNTHRLVAE YGGFLYDSDH YGDDLPFWMD VEVSGGASVP QLIVPYTLDA NDMRFATPQG FNTADHFFHY LRDAFDVLYE EGDEAPKMMS IGMHCRLLGR PGRFRALQRF LDHIERHDRV WVARRVEIAR HWREHHPYRP HARREGDA
Validated NT Sequence
ccccctcgcg ccgggcgtgc ggccgatacg gatgatgctc gcgccagtgc cgcgcgatct cgacgcggcg cgcgacccac acgcgatcgt gccgctcgat gtgatcgaga aagcgctgca gcgcgcgaaa gcgccccggc cggccgagca ggcggcagtg catgccgatc gacatcatct tgggcgcttc gtcgccttcc tcgtacagca cgtcgaacgc gtcgcgcaga tagtggaaga aatgatcggc ggtgttgaag ccctgcggcg tcgcgaaacg catgtcgttc gcgtcgagcg tgtacgggac gatcagttgc ggcacgctcg cgccgcccga cacctcgacg tccatccaga acggcagatc gtcgccgtaa tgatcggagt cgtacaggaa gccgccgtac tcggcgacga gccgatgcgt gttcggactg tcgcggcccg tataccagcc gagcgggcgc acgcccgtca cgcgctcgat cgcctccatt ccgagccgca tgtgctcggc ttcgcgctcg ggcgtcatgt cctgatagtg aatccagcgc cagccgtgac acgcgatctc gtggccgagc tcgacgaacg cgcgcgcgag ctccggatgc cgctcgatcg ccatgccgac gccgaacacc gtgagcggca gcccgcgctt gtcgaactcg cgcaggatgc gccacacgcc cgcgcgcgag ccgtattcgt agatcgactc catgctcatg tggcgcgccg gatacgcggc cgcgccgacg atctcggaca ggaactgctc ggaggcgggg tcgccgtgca gcacgcagtt ctcgccgcct tcctcgtagt tgagcacgaa ctgcaccgcg acgcgcgcgc ggcccggcca gttcgcctgc accggatgtc ggccgtagcc gatcagatcg cgtggatagt tgggatcgaa tgccatcgaa ccaggaccct gggtctgagc ttccagggta ccca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFDPNYPR DLIGYGRHPV QANWPGRARV AVQFVLNYEE GGENCVLHGD PASEQFLSEI VGAAAYPARH MSMESIYEYG SRAGVWRILR EFDKRGLPLT VFGVGMAIER HPELARAFVE LGHEIACHGW RWIHYQDMTP EREAEHMRLG MEAIERVTGV RPLGWYTGRD SPNTHRLVAE YGGFLYDSDH YGDDLPFWMD VEVSGGASVP QLIVPYTLDA NDMRFATPQG FNTADHFFHY LRDAFDVLYE EGDEAPKMMS IGMHCRLLGR PGRFRALQRF LDHIERHDRV WVARRVEIAR HWREHHPYRP HARREGDA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcattcgat cccaactatc cacgcgatct gatcggctac ggccgacatc cggtgcaggc gaactggccg ggccgcgcgc gcgtcgcggt gcagttcgtg ctcaactacg aggaaggcgg cgagaactgc gtgctgcacg gcgaccccgc ctccgagcag ttcctgtccg agatcgtcgg cgcggccgcg tatccggcgc gccacatgag catggagtcg atctacgaat acggctcgcg cgcgggcgtg tggcgcatcc tgcgcgagtt cgacaagcgc gggctgccgc tcacggtgtt cggcgtcggc atggcgatcg agcggcatcc ggagctcgcg cgcgcgttcg tcgagctcgg ccacgagatc gcgtgtcacg gctggcgctg gattcactat caggacatga cgcccgagcg cgaagccgag cacatgcggc tcggaatgga ggcgatcgag cgcgtgacgg gcgtgcgccc gctcggctgg tatacgggcc gcgacagtcc gaacacgcat cggctcgtcg ccgagtacgg cggcttcctg tacgactccg atcattacgg cgacgatctg ccgttctgga tggacgtcga ggtgtcgggc ggcgcgagcg tgccgcaact gatcgtcccg tacacgctcg acgcgaacga catgcgtttc gcgacgccgc agggcttcaa caccgccgat catttcttcc actatctgcg cgacgcgttc gacgtgctgt acgaggaagg cgacgaagcg cccaagatga tgtcgatcgg catgcactgc cgcctgctcg gccggccggg gcgctttcgc gcgctgcagc gctttctcga tcacatcgag cggcacgatc gcgtgtgggt cgcgcgccgc gtcgagatcg cgcggcactg gcgcgagcat catccgtatc ggccgcacgc ccggcgcgag ggggacgcat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.17357.a.A1.PS01018
| PURIFICATION DATe: | 3/25/2011 |
| CONCENTRATION: | 110.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAFDPNYPR DLIGYGRHPV QANWPGRARV AVQFVLNYEE GGENCVLHGD PASEQFLSEI VGAAAYPARH MSMESIYEYG SRAGVWRILR EFDKRGLPLT VFGVGMAIER HPELARAFVE LGHEIACHGW RWIHYQDMTP EREAEHMRLG MEAIERVTGV RPLGWYTGRD SPNTHRLVAE YGGFLYDSDH YGDDLPFWMD VEVSGGASVP QLIVPYTLDA NDMRFATPQG FNTADHFFHY LRDAFDVLYE EGDEAPKMMS IGMHCRLLGR PGRFRALQRF LDHIERHDRV WVARRVEIAR HWREHHPYRP HARREGDA
Validated NT Sequence
ccccctcgcg ccgggcgtgc ggccgatacg gatgatgctc gcgccagtgc cgcgcgatct cgacgcggcg cgcgacccac acgcgatcgt gccgctcgat gtgatcgaga aagcgctgca gcgcgcgaaa gcgccccggc cggccgagca ggcggcagtg catgccgatc gacatcatct tgggcgcttc gtcgccttcc tcgtacagca cgtcgaacgc gtcgcgcaga tagtggaaga aatgatcggc ggtgttgaag ccctgcggcg tcgcgaaacg catgtcgttc gcgtcgagcg tgtacgggac gatcagttgc ggcacgctcg cgccgcccga cacctcgacg tccatccaga acggcagatc gtcgccgtaa tgatcggagt cgtacaggaa gccgccgtac tcggcgacga gccgatgcgt gttcggactg tcgcggcccg tataccagcc gagcgggcgc acgcccgtca cgcgctcgat cgcctccatt ccgagccgca tgtgctcggc ttcgcgctcg ggcgtcatgt cctgatagtg aatccagcgc cagccgtgac acgcgatctc gtggccgagc tcgacgaacg cgcgcgcgag ctccggatgc cgctcgatcg ccatgccgac gccgaacacc gtgagcggca gcccgcgctt gtcgaactcg cgcaggatgc gccacacgcc cgcgcgcgag ccgtattcgt agatcgactc catgctcatg tggcgcgccg gatacgcggc cgcgccgacg atctcggaca ggaactgctc ggaggcgggg tcgccgtgca gcacgcagtt ctcgccgcct tcctcgtagt tgagcacgaa ctgcaccgcg acgcgcgcgc ggcccggcca gttcgcctgc accggatgtc ggccgtagcc gatcagatcg cgtggatagt tgggatcgaa tgccatcgaa ccaggaccct gggtctgagc ttccagggta ccca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFDPNYPR DLIGYGRHPV QANWPGRARV AVQFVLNYEE GGENCVLHGD PASEQFLSEI VGAAAYPARH MSMESIYEYG SRAGVWRILR EFDKRGLPLT VFGVGMAIER HPELARAFVE LGHEIACHGW RWIHYQDMTP EREAEHMRLG MEAIERVTGV RPLGWYTGRD SPNTHRLVAE YGGFLYDSDH YGDDLPFWMD VEVSGGASVP QLIVPYTLDA NDMRFATPQG FNTADHFFHY LRDAFDVLYE EGDEAPKMMS IGMHCRLLGR PGRFRALQRF LDHIERHDRV WVARRVEIAR HWREHHPYRP HARREGDA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCATTCGAT CCCAACTATC CACGCGATCT GATCGGCTAC GGCCGACATC CGGTGCAGGC GAACTGGCCG GGCCGCGCGC GCGTCGCGGT GCAGTTCGTG CTCAACTACG AGGAAGGCGG CGAGAACTGC GTGCTGCACG GCGACCCCGC CTCCGAGCAG TTCCTGTCCG AGATCGTCGG CGCGGCCGCG TATCCGGCGC GCCACATGAG CATGGAGTCG ATCTACGAAT ACGGCTCGCG CGCGGGCGTG TGGCGCATCC TGCGCGAGTT CGACAAGCGC GGGCTGCCGC TCACGGTGTT CGGCGTCGGC ATGGCGATCG AGCGGCATCC GGAGCTCGCG CGCGCGTTCG TCGAGCTCGG CCACGAGATC GCGTGTCACG GCTGGCGCTG GATTCACTAT CAGGACATGA CGCCCGAGCG CGAAGCCGAG CACATGCGGC TCGGAATGGA GGCGATCGAG CGCGTGACGG GCGTGCGCCC GCTCGGCTGG TATACGGGCC GCGACAGTCC GAACACGCAT CGGCTCGTCG CCGAGTACGG CGGCTTCCTG TACGACTCCG ATCATTACGG CGACGATCTG CCGTTCTGGA TGGACGTCGA GGTGTCGGGC GGCGCGAGCG TGCCGCAACT GATCGTCCCG TACACGCTCG ACGCGAACGA CATGCGTTTC GCGACGCCGC AGGGCTTCAA CACCGCCGAT CATTTCTTCC ACTATCTGCG CGACGCGTTC GACGTGCTGT ACGAGGAAGG CGACGAAGCG CCCAAGATGA TGTCGATCGG CATGCACTGC CGCCTGCTCG GCCGGCCGGG GCGCTTTCGC GCGCTGCAGC GCTTTCTCGA TCACATCGAG CGGCACGATC GCGTGTGGGT CGCGCGCCGC GTCGAGATCG CGCGGCACTG GCGCGAGCAT CATCCGTATC GGCCGCACGC CCGGCGCGAG GGGGACGCAT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA