SSGCID: Target Page

BupsA.17351.a

Indole-3-glycerol phosphate synthase

CENTER ID:	BupsA.17351.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17351.a.A1.GE31226	Full length( BupsA.17351.a )		1	261

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17351.a.A1.PS01073	Full length( BupsA.17351.a )		1	261

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.3717
RefSeq:	YP_334943.1
UniProt:	Q3JNB0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDILDKIIT VKREEIAAAL ESAPLEELKV QASARDSRDF VGALRDKHAA GHAAVIAEVK KASPSKGVLR KHFVPADIAR SYAQHGAACL SVLTDERFFQ GSARYLEQAR AACALPVLRK DFIVDAYQVL EARAMGADAI LLIAAALDTP LMIDLEAYAH SLGLAVLVEV HNRGELDEAL KLKTPLVGIN NRNLRTFETT IDTTLGMLDA IPDDRIVVTE SGILSRADVE RMEAAGVHTF LVGEAFMRAE NPGAELARMF F

NT Sequence

ATGAGCGACA TCCTCGACAA AATCATCACC GTCAAGCGCG AAGAGATCGC CGCCGCGCTC GAGAGCGCGC CGCTCGAAGA ACTGAAGGTG CAGGCGTCGG CGCGCGATTC GCGCGACTTC GTCGGCGCGC TGCGCGACAA GCACGCGGCC GGACACGCCG CCGTCATCGC CGAGGTGAAG AAGGCGAGCC CGTCGAAAGG CGTGCTGCGC AAGCATTTCG TGCCGGCCGA CATCGCCCGC TCGTACGCGC AGCACGGCGC CGCATGCCTG TCGGTGCTGA CCGACGAGCG GTTCTTCCAG GGCAGCGCGC GCTATCTCGA ACAGGCGCGC GCCGCGTGCG CCCTGCCCGT GCTGCGCAAG GACTTCATCG TCGACGCGTA CCAGGTGCTC GAAGCGCGCG CGATGGGCGC GGACGCGATC CTGCTGATCG CCGCGGCGCT CGACACGCCG CTGATGATCG ATCTCGAGGC GTACGCACAT TCGCTCGGGC TCGCGGTGCT CGTCGAAGTG CACAACCGCG GCGAACTGGA CGAAGCGTTG AAACTGAAGA CGCCGCTCGT CGGCATCAAC AACCGCAACC TGCGTACATT CGAGACGACG ATCGACACGA CGCTCGGCAT GCTCGACGCG ATTCCGGACG ATCGGATCGT CGTCACCGAA TCCGGCATCC TGTCGCGCGC GGACGTCGAG CGGATGGAGG CGGCGGGCGT GCATACGTTC CTCGTCGGCG AAGCGTTCAT GCGCGCCGAG AACCCGGGCG CGGAACTCGC GCGGATGTTC TTC

Details for BupsA.17351.a.A1.GE31226

HARVESTED ON:	1/11/2011
SEQUENCED ON:	1/18/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MXHHHHHHMG TLEAQTQGPG SMSDILDKII TVKREEIAAA LESAPLEELK VQASARDSRD FVGALRDKHA AGHAAVIAEV KKASPSKGVL RKHFVPADIA RSYAQHGAAC LSVLTDERFF QGSARYLEQA RAACALPVLR KDFIVDAYQV LEARAMGADA ILLIAAALDT PLMIDLEAYA HSLGLAVLVE VHNRGELDEA LKLKTPLVGI NNRNLRTFET TIDTTLGMLD AIPDDRIVVT ESGILSRADV ERMEAAGVHT FLVGEAFMRA ENPGAELARM FF

Validated NT Sequence

ttattagaag aacatccgcg cgagttccgc gcccgggttc tcggcgcgca tgaacgcttc gccgacgagg aacgtatgca cgcccgccgc ctccatccgc tcgacgtccg cgcgcgacag gatgccggat tcggtgacga cgatccgatc gtccggaatc gcgtcgagca tgccgagcgt cgtgtcgatc gtcgtctcga atgtacgcag gttgcggttg ttgatgccga cgagcggcgt cttcagtttc aacgcttcgt ccagttcgcc gcggttgtgc acttcgacga gcaccgcgag cccgagcgaa tgtgcgtacg cctcgagatc gatcatcagc ggcgtgtcga gcgccgcggc gatcagcagg atcgcgtccg cgcccatcgc gcgcgcttcg agcacctggt acgcgtcgac gatgaagtcc ttgcgcagca cgggcagggc gcacgcggcg cgcgcctgtt cgagatagcg cgcgctgccc tggaagaacc gctcgtcggt cagcaccgac aggcatgcgg cgccgtgctg cgcgtacgag cgggcgatgt cggccggcac gaaatgcttg cgcagcacgc ctttcgacgg gctcgccttc ttcacctcgg cgatgacggc ggcgtgtccg gccgcgtgct tgtcgcgcag cgcgccgacg aagtcgcgcg aatcgcgcgc cgacgcctgc accttcagtt cttcgagcgg cgcgctctcg agcgcggcgg cgatctcttc gcgcttgacg gtgatgattt tgtcgaggat gtcgctcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgancc at

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSDILDKII TVKREEIAAA LESAPLEELK VQASARDSRD FVGALRDKHA AGHAAVIAEV KKASPSKGVL RKHFVPADIA RSYAQHGAAC LSVLTDERFF QGSARYLEQA RAACALPVLR KDFIVDAYQV LEARAMGADA ILLIAAALDT PLMIDLEAYA HSLGLAVLVE VHNRGELDEA LKLKTPLVGI NNRNLRTFET TIDTTLGMLD AIPDDRIVVT ESGILSRADV ERMEAAGVHT FLVGEAFMRA ENPGAELARM FF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgacatc ctcgacaaaa tcatcaccgt caagcgcgaa gagatcgccg ccgcgctcga gagcgcgccg ctcgaagaac tgaaggtgca ggcgtcggcg cgcgattcgc gcgacttcgt cggcgcgctg cgcgacaagc acgcggccgg acacgccgcc gtcatcgccg aggtgaagaa ggcgagcccg tcgaaaggcg tgctgcgcaa gcatttcgtg ccggccgaca tcgcccgctc gtacgcgcag cacggcgccg catgcctgtc ggtgctgacc gacgagcggt tcttccaggg cagcgcgcgc tatctcgaac aggcgcgcgc cgcgtgcgcc ctgcccgtgc tgcgcaagga cttcatcgtc gacgcgtacc aggtgctcga agcgcgcgcg atgggcgcgg acgcgatcct gctgatcgcc gcggcgctcg acacgccgct gatgatcgat ctcgaggcgt acgcacattc gctcgggctc gcggtgctcg tcgaagtgca caaccgcggc gaactggacg aagcgttgaa actgaagacg ccgctcgtcg gcatcaacaa ccgcaacctg cgtacattcg agacgacgat cgacacgacg ctcggcatgc tcgacgcgat tccggacgat cggatcgtcg tcaccgaatc cggcatcctg tcgcgcgcgg acgtcgagcg gatggaggcg gcgggcgtgc atacgttcct cgtcggcgaa gcgttcatgc gcgccgagaa cccgggcgcg gaactcgcgc ggatgttctt ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for BupsA.17351.a.A1.PS01073

PURIFICATION DATe:	5/5/2011
CONCENTRATION:	43.28mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGACATC CTCGACAAAA TCATCACCGT CAAGCGCGAA GAGATCGCCG CCGCGCTCGA GAGCGCGCCG CTCGAAGAAC TGAAGGTGCA GGCGTCGGCG CGCGATTCGC GCGACTTCGT CGGCGCGCTG CGCGACAAGC ACGCGGCCGG ACACGCCGCC GTCATCGCCG AGGTGAAGAA GGCGAGCCCG TCGAAAGGCG TGCTGCGCAA GCATTTCGTG CCGGCCGACA TCGCCCGCTC GTACGCGCAG CACGGCGCCG CATGCCTGTC GGTGCTGACC GACGAGCGGT TCTTCCAGGG CAGCGCGCGC TATCTCGAAC AGGCGCGCGC CGCGTGCGCC CTGCCCGTGC TGCGCAAGGA CTTCATCGTC GACGCGTACC AGGTGCTCGA AGCGCGCGCG ATGGGCGCGG ACGCGATCCT GCTGATCGCC GCGGCGCTCG ACACGCCGCT GATGATCGAT CTCGAGGCGT ACGCACATTC GCTCGGGCTC GCGGTGCTCG TCGAAGTGCA CAACCGCGGC GAACTGGACG AAGCGTTGAA ACTGAAGACG CCGCTCGTCG GCATCAACAA CCGCAACCTG CGTACATTCG AGACGACGAT CGACACGACG CTCGGCATGC TCGACGCGAT TCCGGACGAT CGGATCGTCG TCACCGAATC CGGCATCCTG TCGCGCGCGG ACGTCGAGCG GATGGAGGCG GCGGGCGTGC ATACGTTCCT CGTCGGCGAA GCGTTCATGC GCGCCGAGAA CCCGGGCGCG GAACTCGCGC GGATGTTCTT CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA