SSGCID: Target Page

BupsA.17350.b

Flagellar hook-associated protein

CENTER ID:	BupsA.17350.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17350.b.A1.GE31225	Full length( BupsA.17350.b )		1	506

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17350.b.A1.PS01161	Full length( BupsA.17350.b )		1	506

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.88
RefSeq:	YP_331509.1
UniProt:	Q3JY44

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTPVTSTTQ QQTNSALQQA AQSIISGSTG NSSMDVNSLV TALVNAKTAG QSAALSTSIA TDQTTLSALG TLKAALTALQ AGIGSLSDGT LTQKFTATAT GTGLTATTGA GAVAGSYSVA VTQIATSQTL SSGAFNATQQ LGTGTLTLSV GGKSTSISID STNNTLSGIA AAINSASNNP GVTATIVTGT DGAHLVLRSA STGAANVINV GVSNLSGDNG LSSLAVTSTA STTGGQSTIR SGGSVAWTQS TSAQDAEFTV GGIAASSASN AVSGAIAGVT LNLTQAAVGA TQTLNVTTDT TAQATAITNV VNLYNTVITT MSSLSSLSGA GTSSQSAGPL LGDSTLNMIR NSLARVVGAG VTTGGSTTSL ASIGIKFADG SSSSQTDGAL TIDTAKLNAA LQNSPSTVAA LFNSTNGIGA QLNTTIQNYV QTGGVFDTRS NALNQDLKSL AQQQTRLASY ASQLTSQYNA QFTALNTLMA QMNSNSNYLT QLFGGSNSSG AMANNK

NT Sequence

ATGTCCACGC CCGTCACCAG CACGACGCAG CAGCAAACCA ACTCGGCGCT GCAGCAAGCA GCGCAGTCGA TCATCAGCGG CTCGACGGGC AATTCGTCGA TGGACGTCAA CTCGCTCGTC ACCGCGCTCG TCAACGCGAA GACGGCCGGC CAGAGCGCGG CGCTGTCGAC TTCGATCGCG ACCGACCAGA CGACGCTTTC CGCGCTCGGC ACGCTGAAGG CCGCGCTCAC CGCGCTGCAA GCGGGGATCG GCTCGCTCAG CGACGGCACG CTGACCCAGA AATTCACCGC CACGGCAACG GGCACCGGGC TCACCGCGAC GACGGGCGCG GGTGCGGTGG CCGGCAGCTA CTCGGTCGCC GTCACGCAGA TCGCCACGTC GCAGACGCTG TCTTCCGGCG CATTCAATGC GACGCAGCAG CTCGGCACCG GCACGCTGAC GCTGAGCGTC GGCGGCAAAT CGACGTCGAT CTCGATCGAT TCGACGAACA ACACGCTTTC CGGCATCGCC GCCGCGATCA ACTCCGCGTC GAACAACCCC GGCGTGACGG CGACGATCGT CACGGGCACC GACGGCGCGC ACCTCGTGCT GCGCTCGGCA AGCACGGGCG CGGCCAACGT GATCAACGTC GGCGTGAGCA ACCTGTCCGG CGACAACGGG CTGTCGAGCC TCGCCGTCAC GTCGACGGCG AGCACGACGG GCGGCCAGTC GACGATCCGC TCGGGCGGCA GCGTCGCATG GACGCAAAGC ACCTCCGCTC AGGACGCCGA ATTCACGGTG GGCGGCATCG CCGCGTCGAG CGCGAGCAAC GCAGTGTCGG GTGCGATCGC CGGCGTCACG CTGAACCTCA CGCAAGCCGC CGTCGGCGCC ACGCAGACGC TGAACGTGAC CACCGACACC ACCGCGCAGG CCACCGCGAT CACGAACGTC GTCAACCTGT ACAACACGGT GATCACGACG ATGTCGTCGC TGTCGTCGCT ATCCGGCGCG GGCACCAGCT CGCAAAGCGC GGGGCCGCTC CTCGGCGACT CCACGCTCAA CATGATCCGC AACTCGCTCG CGCGCGTGGT GGGCGCGGGC GTGACGACGG GCGGCTCGAC CACGTCGCTC GCGTCGATCG GCATCAAGTT CGCCGACGGC TCGTCGTCGT CGCAGACGGA CGGCGCACTG ACCATCGATA CGGCCAAGCT CAACGCCGCG CTGCAAAACA GCCCGTCGAC CGTCGCCGCG CTCTTCAATT CGACGAACGG CATCGGCGCG CAGCTGAACA CCACGATCCA GAACTATGTG CAGACGGGCG GCGTCTTCGA TACGCGCTCG AACGCGCTGA ACCAGGACCT GAAGAGCCTC GCGCAGCAGC AGACGCGGCT CGCGTCGTAC GCGTCGCAAC TCACGTCGCA ATACAACGCG CAGTTCACCG CGCTCAACAC GCTGATGGCG CAGATGAACA GCAACTCGAA CTACCTGACG CAGCTGTTCG GCGGCAGCAA CAGCTCGGGC GCGATGGCGA ACAACAAG

Details for BupsA.17350.b.A1.GE31225

HARVESTED ON:	1/11/2011
SEQUENCED ON:	1/18/2011
EXPECTED MW:	51kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	29

Validated AA Sequence

MIRNSLARVV GAGVTTGGST TSLASIGIKF ADGSSSSQTD GALTIDTAKL NAALQNSPST VAALFNSTNG IGAQLNTTIQ NYVQTGGVFD TRSNALNQDL KSLAQQQTRL ASYASQLTSQ YNAQFTALNT LMAQMNSNSN YLTQLFGGSN SSGAMAN

Validated NT Sequence

tgttcgccat cgcgcccgag ctgttgctgc cgccgaacag ctgcgtcagg tagttcgagt tgctgttcat ctgcgccatc agcgtgttga gcgcggtgaa ctgcgcgttg tattgcgacg tgagttgcga cgcgtacgac gcgagccgcg tctgctgctg cgcgaggctc ttcaggtcct ggttcagcgc gttcgagcgc gtatcgaaga cgccgcccgt ctgcacatag ttctggatcg tggtgttcag ctgcgcgccg atgccgttcg tcgaattgaa gagcgcggcg acggtcgacg ggctgttttg cagcgcggcg ttgagcttgg ccgtatcgat ggtcagtgcg ccgtccgtct gcgacgacga cgagccgtcg gcgaacttga tgccgatcga cgcgagcgac gtggtcgagc cgcccgtcgt cacgcccgcg cccaccacgc gcgcgagcga gttgcggatc atgttgagcg tggagtcgcc gaggagcggc cccgcgcttt gcgagctggt gcccgcgccg gatagcgacg acngcgacga cntcgtcgtg atcaccgtgt tgtacaggtt gacgacgttc gtgatcgcgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTPVTSTT QQQTNSALQQ AAQSIISGST GNSSMDVNSL VTALVNAKTA GQSAALSTSI ATDQTTLSAL GTLKAALTAL QAGIGSLSDG TLTQKFTATA TGTGLTATTG AGAVAGSYSV AVTQIATSQT LSSGAFNATQ QLGTGTLTLS VGGKSTSISI DSTNNTLSGI AAAINSASNN PGVTATIVTG TDGAHLVLRS ASTGAANVIN VGVSNLSGDN GLSSLAVTST ASTTGGQSTI RSGGSVAWTQ STSAQDAEFT VGGIAASSAS NAVSGAIAGV TLNLTQAAVG ATQTLNVTTD TTAQATAITN VVNLYNTVIT TMSSLSSLSG AGTSSQSAGP LLGDSTLNMI RNSLARVVGA GVTTGGSTTS LASIGIKFAD GSSSSQTDGA LTIDTAKLNA ALQNSPSTVA ALFNSTNGIG AQLNTTIQNY VQTGGVFDTR SNALNQDLKS LAQQQTRLAS YASQLTSQYN AQFTALNTLM AQMNSNSNYL TQLFGGSNSS GAMANNK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccacgccc gtcaccagca cgacgcagca gcaaaccaac tcggcgctgc agcaagcagc gcagtcgatc atcagcggct cgacgggcaa ttcgtcgatg gacgtcaact cgctcgtcac cgcgctcgtc aacgcgaaga cggccggcca gagcgcggcg ctgtcgactt cgatcgcgac cgaccagacg acgctttccg cgctcggcac gctgaaggcc gcgctcaccg cgctgcaagc ggggatcggc tcgctcagcg acggcacgct gacccagaaa ttcaccgcca cggcaacggg caccgggctc accgcgacga cgggcgcggg tgcggtggcc ggcagctact cggtcgccgt cacgcagatc gccacgtcgc agacgctgtc ttccggcgca ttcaatgcga cgcagcagct cggcaccggc acgctgacgc tgagcgtcgg cggcaaatcg acgtcgatct cgatcgattc gacgaacaac acgctttccg gcatcgccgc cgcgatcaac tccgcgtcga acaaccccgg cgtgacggcg acgatcgtca cgggcaccga cggcgcgcac ctcgtgctgc gctcggcaag cacgggcgcg gccaacgtga tcaacgtcgg cgtgagcaac ctgtccggcg acaacgggct gtcgagcctc gccgtcacgt cgacggcgag cacgacgggc ggccagtcga cgatccgctc gggcggcagc gtcgcatgga cgcaaagcac ctccgctcag gacgccgaat tcacggtggg cggcatcgcc gcgtcgagcg cgagcaacgc agtgtcgggt gcgatcgccg gcgtcacgct gaacctcacg caagccgccg tcggcgccac gcagacgctg aacgtgacca ccgacaccac cgcgcaggcc accgcgatca cgaacgtcgt caacctgtac aacacggtga tcacgacgat gtcgtcgctg tcgtcgctat ccggcgcggg caccagctcg caaagcgcgg ggccgctcct cggcgactcc acgctcaaca tgatccgcaa ctcgctcgcg cgcgtggtgg gcgcgggcgt gacgacgggc ggctcgacca cgtcgctcgc gtcgatcggc atcaagttcg ccgacggctc gtcgtcgtcg cagacggacg gcgcactgac catcgatacg gccaagctca acgccgcgct gcaaaacagc ccgtcgaccg tcgccgcgct cttcaattcg acgaacggca tcggcgcgca gctgaacacc acgatccaga actatgtgca gacgggcggc gtcttcgata cgcgctcgaa cgcgctgaac caggacctga agagcctcgc gcagcagcag acgcggctcg cgtcgtacgc gtcgcaactc acgtcgcaat acaacgcgca gttcaccgcg ctcaacacgc tgatggcgca gatgaacagc aactcgaact acctgacgca gctgttcggc ggcagcaaca gctcgggcgc gatggcgaac aacaagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BupsA.17350.b.A1.PS01161

PURIFICATION DATe:	7/20/2011
CONCENTRATION:	21.4mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	29

Protocol Notes

notes unavailable

Validated AA Sequence

MIRNSLARVV GAGVTTGGST TSLASIGIKF ADGSSSSQTD GALTIDTAKL NAALQNSPST VAALFNSTNG IGAQLNTTIQ NYVQTGGVFD TRSNALNQDL KSLAQQQTRL ASYASQLTSQ YNAQFTALNT LMAQMNSNSN YLTQLFGGSN SSGAMAN

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCACGCCC GTCACCAGCA CGACGCAGCA GCAAACCAAC TCGGCGCTGC AGCAAGCAGC GCAGTCGATC ATCAGCGGCT CGACGGGCAA TTCGTCGATG GACGTCAACT CGCTCGTCAC CGCGCTCGTC AACGCGAAGA CGGCCGGCCA GAGCGCGGCG CTGTCGACTT CGATCGCGAC CGACCAGACG ACGCTTTCCG CGCTCGGCAC GCTGAAGGCC GCGCTCACCG CGCTGCAAGC GGGGATCGGC TCGCTCAGCG ACGGCACGCT GACCCAGAAA TTCACCGCCA CGGCAACGGG CACCGGGCTC ACCGCGACGA CGGGCGCGGG TGCGGTGGCC GGCAGCTACT CGGTCGCCGT CACGCAGATC GCCACGTCGC AGACGCTGTC TTCCGGCGCA TTCAATGCGA CGCAGCAGCT CGGCACCGGC ACGCTGACGC TGAGCGTCGG CGGCAAATCG ACGTCGATCT CGATCGATTC GACGAACAAC ACGCTTTCCG GCATCGCCGC CGCGATCAAC TCCGCGTCGA ACAACCCCGG CGTGACGGCG ACGATCGTCA CGGGCACCGA CGGCGCGCAC CTCGTGCTGC GCTCGGCAAG CACGGGCGCG GCCAACGTGA TCAACGTCGG CGTGAGCAAC CTGTCCGGCG ACAACGGGCT GTCGAGCCTC GCCGTCACGT CGACGGCGAG CACGACGGGC GGCCAGTCGA CGATCCGCTC GGGCGGCAGC GTCGCATGGA CGCAAAGCAC CTCCGCTCAG GACGCCGAAT TCACGGTGGG CGGCATCGCC GCGTCGAGCG CGAGCAACGC AGTGTCGGGT GCGATCGCCG GCGTCACGCT GAACCTCACG CAAGCCGCCG TCGGCGCCAC GCAGACGCTG AACGTGACCA CCGACACCAC CGCGCAGGCC ACCGCGATCA CGAACGTCGT CAACCTGTAC AACACGGTGA TCACGACGAT GTCGTCGCTG TCGTCGCTAT CCGGCGCGGG CACCAGCTCG CAAAGCGCGG GGCCGCTCCT CGGCGACTCC ACGCTCAACA TGATCCGCAA CTCGCTCGCG CGCGTGGTGG GCGCGGGCGT GACGACGGGC GGCTCGACCA CGTCGCTCGC GTCGATCGGC ATCAAGTTCG CCGACGGCTC GTCGTCGTCG CAGACGGACG GCGCACTGAC CATCGATACG GCCAAGCTCA ACGCCGCGCT GCAAAACAGC CCGTCGACCG TCGCCGCGCT CTTCAATTCG ACGAACGGCA TCGGCGCGCA GCTGAACACC ACGATCCAGA ACTATGTGCA GACGGGCGGC GTCTTCGATA CGCGCTCGAA CGCGCTGAAC CAGGACCTGA AGAGCCTCGC GCAGCAGCAG ACGCGGCTCG CGTCGTACGC GTCGCAACTC ACGTCGCAAT ACAACGCGCA GTTCACCGCG CTCAACACGC TGATGGCGCA GATGAACAGC AACTCGAACT ACCTGACGCA GCTGTTCGGC GGCAGCAACA GCTCGGGCGC GATGGCGAAC AACAAGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA