SSGCID: Target Page

BupsA.17348.b

Predicted esterase of the alpha/beta hydrolase fold

CENTER ID:	BupsA.17348.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17348.b.A1.GE31221	Full length( BupsA.17348.b )		1	209

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.548
RefSeq:	YP_331960.1
UniProt:	Q3JWU3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRSCSKSTWP PRLVTAPGLH GSEGAHWQTW LERQFARALR VEQDDWDAPH VERWAQKVRD LLARERGPFV IAAHSFGCLA TAHALAQAAQ TRAADVVGVL FVAPANPRKF AFAGDFDARR LNVPSIVVGS ESDPWMTLAD AREFAQRLGS AFVNLGDAGH INTAAGYGPW PRAKYFVDTL VHCAAPLRLR DDDAPASGAP ARQPLVTAA

NT Sequence

ATGCGCTCAT GCAGCAAATC GACGTGGCCG CCGCGGCTCG TCACGGCGCC CGGCCTGCAC GGCAGCGAAG GCGCGCACTG GCAGACCTGG CTCGAGAGGC AGTTCGCGCG TGCGCTGCGC GTCGAGCAGG ACGACTGGGA CGCGCCGCAT GTCGAGCGCT GGGCGCAGAA GGTGCGCGAT CTGCTCGCGC GCGAGCGCGG GCCGTTCGTG ATCGCCGCGC ACAGCTTCGG CTGCCTCGCG ACCGCGCATG CGCTCGCGCA GGCCGCGCAG ACGCGGGCGG CGGACGTCGT CGGCGTGCTG TTCGTCGCGC CGGCGAATCC GCGCAAGTTC GCGTTCGCGG GCGATTTCGA CGCGCGGCGC CTGAACGTGC CGTCGATCGT GGTCGGCAGC GAAAGCGATC CATGGATGAC GCTCGCCGAC GCGCGCGAGT TCGCGCAACG GCTCGGCAGC GCGTTCGTCA ATCTCGGCGA CGCGGGGCAC ATCAACACCG CGGCGGGTTA CGGTCCGTGG CCGCGCGCGA AATACTTCGT CGACACGCTC GTGCACTGCG CGGCGCCGCT CAGGTTGCGC GACGACGATG CGCCGGCGTC CGGCGCGCCC GCGCGGCAGC CGCTCGTGAC GGCCGCT

Details for BupsA.17348.b.A1.GE31221

HARVESTED ON:	1/11/2011
SEQUENCED ON:	1/18/2011
EXPECTED MW:	24kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRSCSKSTW PPRLVTAPGL HGSEGAHWQT WLERQFARAL RVEQDDWDAP HVERWAQKVR DLLARERGPF VIAAHSFGCL ATAHALAQAA QTRAADVVGV LFVAPANPRK FAFAGDFDAR RLNVPSIVVG SESDPWMTLA DAREFAQRLG SAFVNLGDAG HINTAAGYGP WPRAKYFVDT LVHCAAPLRL RDDDAPASGA PARQPLVTAA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgct catgcagcaa atcgacgtgg ccgccgcggc tcgtcacggc gcccggcctg cacggcagcg aaggcgcgca ctggcagacc tggctcgaga ggcagttcgc gcgtgcgctg cgcgtcgagc aggacgactg ggacgcgccg catgtcgagc gctgggcgca gaaggtgcgc gatctgctcg cgcgcgagcg cgggccgttc gtgatcgccg cgcacagctt cggctgcctc gcgaccgcgc atgcgctcgc gcaggccgcg cagacgcggg cggcggacgt cgtcggcgtg ctgttcgtcg cgccggcgaa tccgcgcaag ttcgcgttcg cgggcgattt cgacgcgcgg cgcctgaacg tgccgtcgat cgtggtcggc agcgaaagcg atccatggat gacgctcgcc gacgcgcgcg agttcgcgca acggctcggc agcgcgttcg tcaatctcgg cgacgcgggg cacatcaaca ccgcggcggg ttacggtccg tggccgcgcg cgaaatactt cgtcgacacg ctcgtgcact gcgcggcgcc gctcaggttg cgcgacgacg atgcgccggc gtccggcgcg cccgcgcggc agccgctcgt gacggccgct taat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgctcatgc agcaaatcga cgtggccgcc gcggctcgtc acggcgcccg gcctgcacgg cagcgaaggc gcgcactggc agacctggct cgagaggcag ttcgcgcgtg cgctgcgcgt cgagcaggac gactgggacg cgccgcatgt cgagcgctgg gcgcagaagg tgcgcgatct gctcgcgcgc gagcgcgggc cgttcgtgat cgccgcgcac agcttcggct gcctcgcgac cgcgcatgcg ctcgcgcagg ccgcgcagac gcgggcggcg gacgtcgtcg gcgtgctgtt cgtcgcgccg gcgaatccgc gcaagttcgc gttcgcgggc gatttcgacg cgcggcgcct gaacgtgccg tcgatcgtgg tcggcagcga aagcgatcca tggatgacgc tcgccgacgc gcgcgagttc gcgcaacggc tcggcagcgc gttcgtcaat ctcggcgacg cggggcacat caacaccgcg gcgggttacg gtccgtggcc gcgcgcgaaa tacttcgtcg acacgctcgt gcactgcgcg gcgccgctca ggttgcgcga cgacgatgcg ccggcgtccg gcgcgcccgc gcggcagccg ctcgtgacgg ccgcttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa