BupsA.17346.a

MlrC C-terminus family

CENTER ID: BupsA.17346.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17346.a.A1.GE31217 Full length( BupsA.17346.a ) 1 518
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.5425
RefSeq: YP_336483.1
UniProt: Q3JIX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MERRRIMKIL VAGFRHESNT FAPSKATYAS FAADGGRYPL SRGAEIGRLK RMNLPVAGAL AALADAGHVA LPAVWADATP SGRVESVAFE RIAGEIVDAA KRYDADGVYV DLHGAMATER YDDGEGELLR RLRETVGARV PIVASLDLHA NVTQRMLDSA DGLVTYRTYP HVDMADTGRR AVALLDTLLG RRGRHRHFRS ARRVPFLIPV NAMCTSLEPS KSLFRLLERL ETGAVRSLSF APGFPAADFP ECGPTIWGYG ADPVQLARAV DALYEHVVST EAQWSVPFMS ADDAVTEAIR IARGADKPVV IADTQDNPGA GGGSNTTGLL RALVRHRAPD AALGLFFDPA AACAAHAAGR GATVEITLGA DSGLPFTGTF RVESLSNGRC HCNGPMLKGA TFELGPTACL RIGDVRVVVT SARVQMTDRS FYRIAGIAPE TMKILVNKSS VHFRADFDAI ADCVLIAKAG GWMAADPADL RWTSLADGIR TSPCGSPFFG CGGRRAPHAD GIAGEMRI
NT Sequence
ATGGAGCGCC GTCGCATCAT GAAGATCCTG GTCGCCGGCT TTCGGCACGA GTCGAACACG TTCGCACCCA GCAAGGCGAC CTACGCGAGC TTCGCGGCGG ACGGCGGCCG CTATCCGCTG TCGCGCGGCG CCGAGATCGG CCGGCTCAAG CGGATGAACC TGCCCGTCGC GGGCGCGCTC GCCGCGCTCG CCGACGCCGG CCACGTCGCG CTGCCCGCGG TCTGGGCCGA TGCGACGCCG TCGGGCCGCG TCGAATCGGT GGCGTTCGAG CGGATCGCGG GCGAGATCGT CGATGCCGCG AAGCGCTACG ACGCCGACGG CGTCTACGTC GATCTGCACG GCGCGATGGC GACCGAGCGC TACGACGACG GCGAGGGCGA GCTGCTGCGC CGGCTGCGCG AGACGGTGGG CGCGCGCGTG CCGATCGTCG CGTCGCTCGA TCTGCACGCG AACGTCACGC AGCGCATGCT CGACAGCGCG GACGGGCTCG TCACGTACCG CACGTATCCG CACGTCGACA TGGCCGATAC CGGGCGCCGC GCGGTCGCGC TGCTCGACAC GCTGCTCGGC AGGCGCGGCC GCCACCGCCA TTTCCGCAGC GCGCGGCGCG TGCCGTTCCT GATCCCGGTG AACGCGATGT GCACGTCGCT CGAGCCGTCG AAGAGCCTGT TCAGGCTGCT CGAGCGGCTC GAGACGGGCG CCGTGCGCTC GCTGTCGTTC GCGCCGGGCT TTCCGGCCGC GGACTTCCCG GAATGCGGGC CGACGATCTG GGGTTACGGC GCGGACCCCG TCCAGCTCGC GCGCGCGGTC GACGCGCTGT ACGAGCACGT CGTGTCGACC GAGGCGCAGT GGTCGGTGCC GTTCATGTCG GCGGACGACG CGGTGACCGA GGCGATCCGG ATCGCGCGCG GCGCGGACAA GCCGGTCGTG ATCGCCGACA CGCAGGACAA CCCGGGCGCG GGCGGCGGCT CGAACACGAC GGGGCTGTTG CGCGCGCTCG TGCGGCACCG CGCGCCCGAT GCGGCGCTCG GGCTGTTCTT CGATCCGGCG GCCGCGTGCG CCGCGCATGC GGCAGGCCGC GGCGCGACGG TCGAGATCAC GCTCGGCGCG GACAGCGGGC TGCCGTTTAC CGGGACGTTT CGCGTCGAAT CGCTGTCGAA CGGCCGCTGC CATTGCAACG GCCCGATGCT CAAGGGCGCG ACGTTCGAGC TCGGCCCGAC CGCGTGCCTG CGGATCGGCG ACGTGCGCGT CGTCGTCACG TCGGCGCGCG TGCAGATGAC CGACCGGAGC TTCTATCGGA TCGCGGGCAT CGCGCCCGAG ACGATGAAGA TCCTGGTCAA CAAGAGCTCC GTTCATTTTC GGGCGGATTT CGATGCGATC GCAGATTGCG TGCTGATCGC GAAAGCGGGC GGCTGGATGG CCGCCGACCC GGCCGATCTG CGCTGGACGT CGCTTGCCGA CGGGATACGC ACGAGCCCGT GCGGCTCGCC GTTCTTCGGC TGCGGCGGGC GGCGCGCGCC GCATGCGGAC GGGATCGCGG GAGAGATGCG GATA
Details for BupsA.17346.a.A1.GE31217
HARVESTED ON: 1/11/2011
SEQUENCED ON: 1/18/2011
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 28
PERCENT COVERAGE: 33
Validated AA Sequence
MFTXTPXXXX XXXQLXXXXX QXXXXXXXXX XXAXPXXGXX XFXXXXSXGX XCXXXXXXXX XXXXXXXXXX XXXXXLXVAG ALAXXXDAXH XALPAVWADA TPSGXVXXVA FXXIAGEIVX AAKXXXXXGX XXXLXXXMXT XXXXXXXXSX CSGCARRWAR XXXXSXXSXC TXXXXXXX
Validated NT Sequence
gcggnncagc catgtttacg nnnactcctn nnnnnancca nnnnnnnnna caactngnan nnannannat ncaannnnnn nnntnnnnnn nnnnnnnnnc nnccgnngcn gncccnngtg ncggcnntan nnanttctnn nnccannttt cncnnggnnc cgnntgctnn nagcnnnnan gnngnnngnn nnngnnnnnn nnnnnntnac nnnnnnnnng gntcnngngg angancctgn ccgtcgcggg cgcgctcgcc gngnnnnncg acgccngcca cgncgcgctg cccgcggtct gggccgatgc gacgccgtcg ggccnngtcn annnngtggc gttcnancnn atcgcgggcg agatcgtcna tgccgcnaag cncnnnnacg ncganggcnt ctnnnnnnat ctgcncngcn cgatgncnac ngannnnnnn nacnacgnnn nnnngagctg ntgctcnggn tgcgcgagac ggtgggcncg cnngnnnnnn nnntcnnntc nntcgntctg cacnnnncnn nnnnnnnnnn nngntcga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMERRRIMKI LVAGFRHESN TFAPSKATYA SFAADGGRYP LSRGAEIGRL KRMNLPVAGA LAALADAGHV ALPAVWADAT PSGRVESVAF ERIAGEIVDA AKRYDADGVY VDLHGAMATE RYDDGEGELL RRLRETVGAR VPIVASLDLH ANVTQRMLDS ADGLVTYRTY PHVDMADTGR RAVALLDTLL GRRGRHRHFR SARRVPFLIP VNAMCTSLEP SKSLFRLLER LETGAVRSLS FAPGFPAADF PECGPTIWGY GADPVQLARA VDALYEHVVS TEAQWSVPFM SADDAVTEAI RIARGADKPV VIADTQDNPG AGGGSNTTGL LRALVRHRAP DAALGLFFDP AAACAAHAAG RGATVEITLG ADSGLPFTGT FRVESLSNGR CHCNGPMLKG ATFELGPTAC LRIGDVRVVV TSARVQMTDR SFYRIAGIAP ETMKILVNKS SVHFRADFDA IADCVLIAKA GGWMAADPAD LRWTSLADGI RTSPCGSPFF GCGGRRAPHA DGIAGEMRI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagcgccgt cgcatcatga agatcctggt cgccggcttt cggcacgagt cgaacacgtt cgcacccagc aaggcgacct acgcgagctt cgcggcggac ggcggccgct atccgctgtc gcgcggcgcc gagatcggcc ggctcaagcg gatgaacctg cccgtcgcgg gcgcgctcgc cgcgctcgcc gacgccggcc acgtcgcgct gcccgcggtc tgggccgatg cgacgccgtc gggccgcgtc gaatcggtgg cgttcgagcg gatcgcgggc gagatcgtcg atgccgcgaa gcgctacgac gccgacggcg tctacgtcga tctgcacggc gcgatggcga ccgagcgcta cgacgacggc gagggcgagc tgctgcgccg gctgcgcgag acggtgggcg cgcgcgtgcc gatcgtcgcg tcgctcgatc tgcacgcgaa cgtcacgcag cgcatgctcg acagcgcgga cgggctcgtc acgtaccgca cgtatccgca cgtcgacatg gccgataccg ggcgccgcgc ggtcgcgctg ctcgacacgc tgctcggcag gcgcggccgc caccgccatt tccgcagcgc gcggcgcgtg ccgttcctga tcccggtgaa cgcgatgtgc acgtcgctcg agccgtcgaa gagcctgttc aggctgctcg agcggctcga gacgggcgcc gtgcgctcgc tgtcgttcgc gccgggcttt ccggccgcgg acttcccgga atgcgggccg acgatctggg gttacggcgc ggaccccgtc cagctcgcgc gcgcggtcga cgcgctgtac gagcacgtcg tgtcgaccga ggcgcagtgg tcggtgccgt tcatgtcggc ggacgacgcg gtgaccgagg cgatccggat cgcgcgcggc gcggacaagc cggtcgtgat cgccgacacg caggacaacc cgggcgcggg cggcggctcg aacacgacgg ggctgttgcg cgcgctcgtg cggcaccgcg cgcccgatgc ggcgctcggg ctgttcttcg atccggcggc cgcgtgcgcc gcgcatgcgg caggccgcgg cgcgacggtc gagatcacgc tcggcgcgga cagcgggctg ccgtttaccg ggacgtttcg cgtcgaatcg ctgtcgaacg gccgctgcca ttgcaacggc ccgatgctca agggcgcgac gttcgagctc ggcccgaccg cgtgcctgcg gatcggcgac gtgcgcgtcg tcgtcacgtc ggcgcgcgtg cagatgaccg accggagctt ctatcggatc gcgggcatcg cgcccgagac gatgaagatc ctggtcaaca agagctccgt tcattttcgg gcggatttcg atgcgatcgc agattgcgtg ctgatcgcga aagcgggcgg ctggatggcc gccgacccgg ccgatctgcg ctggacgtcg cttgccgacg ggatacgcac gagcccgtgc ggctcgccgt tcttcggctg cggcgggcgg cgcgcgccgc atgcggacgg gatcgcggga gagatgcgga tataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa