BupsA.17344.b

HrpF

CENTER ID: BupsA.17344.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17344.b.A1.GE31214 Full length( BupsA.17344.b ) 1 289
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.4427
RefSeq: YP_335582.1
UniProt: Q3JLH1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIWLRRPRI AEMGPITPNS CARLGFSGDV VPRECFGELM TIESAYAALD ADRDAVLAAA RQEADRVMAE AVARTEEMIA AAQSEYDAAA ERGYRDGYDQ AFGKWMDRLA DVADAQNRLQ LRMRERLADI VASAVEQIVR AESRETLFKR ALASVERIVD GATYLRVVVH ENDLDQARAI FGTLEARWRE FGRPIAMSVV ADKRLAPGSC VCESDFGAID ASLDTQLRAM RGAVARALKR SIEEAGANEC DATRRDESIG IETDRDGERD ARNAEQDAGD AGASAGGRA
NT Sequence
ATGGCTATTT GGTTGAGACG TCCCCGGATC GCTGAAATGG GGCCGATCAC GCCGAACAGC TGCGCTCGAC TCGGCTTTTC CGGCGATGTT GTGCCGCGCG AATGCTTTGG TGAACTGATG ACGATCGAGT CCGCCTATGC GGCGCTCGAC GCGGACCGCG ACGCCGTGCT CGCTGCCGCC CGTCAGGAAG CCGACAGAGT GATGGCGGAG GCAGTGGCGA GAACGGAAGA GATGATCGCC GCCGCACAGA GCGAATACGA TGCGGCTGCC GAGCGCGGCT ATCGAGACGG CTACGACCAA GCGTTCGGCA AGTGGATGGA TCGCTTAGCC GATGTCGCGG ACGCGCAGAA CCGACTGCAA CTGCGAATGC GCGAGCGGCT GGCCGACATC GTCGCATCGG CGGTCGAGCA AATCGTGCGC GCCGAAAGCC GCGAGACGCT ATTCAAGCGC GCGTTGGCAT CGGTCGAGCG AATCGTGGAC GGCGCGACAT ATTTGCGTGT CGTCGTGCAC GAGAACGATC TGGATCAGGC GAGAGCGATC TTCGGCACGC TCGAAGCGCG CTGGCGCGAG TTCGGCCGCC CGATCGCGAT GTCGGTGGTG GCGGACAAGC GGCTTGCTCC AGGCAGTTGC GTATGCGAGT CCGATTTCGG CGCAATCGAT GCGAGCCTGG ATACGCAGCT GCGCGCGATG CGCGGCGCCG TCGCGCGTGC GCTCAAGCGT TCGATTGAAG AAGCCGGCGC GAACGAGTGC GATGCGACAC GCCGTGACGA ATCGATCGGC ATCGAGACGG ATCGGGACGG CGAGCGTGAC GCACGGAACG CCGAGCAGGA TGCGGGGGAC GCGGGCGCGA GCGCGGGGGG GCGGGCA
Details for BupsA.17344.b.A1.GE31214
HARVESTED ON: 1/11/2011
SEQUENCED ON: 1/18/2011
EXPECTED MW: 33kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMAIWLRR PRIAEMGPIT PNSCARLGFS GDVVPRECFG ELMTIESAYA ALDADRDAVL AAARQEADRV MAEAVARTEE MIAAAQSEYD AAAERGYRDG YDQAFGKWMD RLADVADAQN RLQLRMRERL ADIVASAVEQ IVRAESRETL FKRALASVER IVDGATYLRV VVHENDLDQA RAIFGTLEAR WREFGRPIAM SVVADKRLAP GSCVCESDFG AIDASLDTQL RAMRGAVARA LKRSIEEAGA NECDATRRDE SIGIETDRDG ERDARNAEQD AGDAGASAGG RA
Validated NT Sequence
ttattatgcc cgcccccccg cgctcgcgcc cgcgtccccc gcatcctgct cggcgttccg tgcgtcacgc tcgccgtccc gatccgtctc gatgccgatc gattcgtcac ggcgtgtcgc atcgcactcg ttcgcgccgg cttcttcaat cgaacgcttg agcgcacgcg cgacggcgcc gcgcatcgcg cgcagctgcg tatccaggct cgcatcgatt gcgccgaaat cggactcgca tacgcaactg cctggagcaa gccgcttgtc cgccaccacc gacatcgcga tcgggcggcc gaactcgcgc cagcgcgctt cgagcgtgcc gaagatcgct ctcgcctgat ccagatcgtt ctcgtgcacg acgacacgca aatatgtcgc gccgtccacg attcgctcga ccgatgccaa cgcgcgcttg aatagcgtct cgcggctttc ggcgcgcacg atttgctcga ccgccgatgc gacgatgtcg gccagccgct cgcgcattcg cagttgcagt cggttctgcg cgtccgcgac atcggctaag cgatccatcc acttgccgaa cgcttggtcg tagccgtctc gatagccgcg ctcggcagcc gcatcgtatt cgctctgtgc ggcggcgatc atctcttccg ttctcgccac tgcctccgcc atcactctgt cggcttcctg acgggcggca gcgagcacgg cgtcgcggtc cgcgtcgagc gccgcatagg cggactcgat cgtcatcagt tcaccaaagc attcgcgcgg cacaacatcg ccggaaaagc cgagtcgagc gcagctgttc ggcgtgatcg gccccatttc agcgatccgg ggacgtctca accaaatagc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggnga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAIWLRRPR IAEMGPITPN SCARLGFSGD VVPRECFGEL MTIESAYAAL DADRDAVLAA ARQEADRVMA EAVARTEEMI AAAQSEYDAA AERGYRDGYD QAFGKWMDRL ADVADAQNRL QLRMRERLAD IVASAVEQIV RAESRETLFK RALASVERIV DGATYLRVVV HENDLDQARA IFGTLEARWR EFGRPIAMSV VADKRLAPGS CVCESDFGAI DASLDTQLRA MRGAVARALK RSIEEAGANE CDATRRDESI GIETDRDGER DARNAEQDAG DAGASAGGRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctatttgg ttgagacgtc cccggatcgc tgaaatgggg ccgatcacgc cgaacagctg cgctcgactc ggcttttccg gcgatgttgt gccgcgcgaa tgctttggtg aactgatgac gatcgagtcc gcctatgcgg cgctcgacgc ggaccgcgac gccgtgctcg ctgccgcccg tcaggaagcc gacagagtga tggcggaggc agtggcgaga acggaagaga tgatcgccgc cgcacagagc gaatacgatg cggctgccga gcgcggctat cgagacggct acgaccaagc gttcggcaag tggatggatc gcttagccga tgtcgcggac gcgcagaacc gactgcaact gcgaatgcgc gagcggctgg ccgacatcgt cgcatcggcg gtcgagcaaa tcgtgcgcgc cgaaagccgc gagacgctat tcaagcgcgc gttggcatcg gtcgagcgaa tcgtggacgg cgcgacatat ttgcgtgtcg tcgtgcacga gaacgatctg gatcaggcga gagcgatctt cggcacgctc gaagcgcgct ggcgcgagtt cggccgcccg atcgcgatgt cggtggtggc ggacaagcgg cttgctccag gcagttgcgt atgcgagtcc gatttcggcg caatcgatgc gagcctggat acgcagctgc gcgcgatgcg cggcgccgtc gcgcgtgcgc tcaagcgttc gattgaagaa gccggcgcga acgagtgcga tgcgacacgc cgtgacgaat cgatcggcat cgagacggat cgggacggcg agcgtgacgc acggaacgcc gagcaggatg cgggggacgc gggcgcgagc gcgggggggc gggcataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa