BupsA.17338.b

Capsule polysaccharide biosynthesis protein family

CENTER ID: BupsA.17338.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17338.b.A1.GE31164 Full length( BupsA.17338.b ) 1 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3405
RefSeq: YP_334640.1
UniProt: Q3JP63

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIVVVVDSME RYYFAVRLVK AVRKEFDFLF AASEPLAHLM ALAAGFRSVY LRRGAHAPAM LDAAAGIRSD ASIEVLNGQM TPERARADAQ AVFAAMSGVF RRHLVSQCLM WNGQQLVCRA VAHACAAHGV PTKFVEISNL PDKLFVDRLG VNALSSISRN PAVIDGLPMP TEGEHRRWFA RYEAYKARPL PQSRTSWARK AMSAANYALK LATQGVARKR LDAARATNGA RAPAQAKVLS TKELSALRYV FLPLQVSGDT QIKLHSDVDN LKAIRLAFEH AANENADLIV KLHPAERDVA VIDEVVRMQR VYHFDLVTSP TTDLIKHAHS VVTINSTVGL EALLYGKPVV SLGRCFYKEF DRARLLKYIH AFLIDGIDYF GRADIAPRAA RNVFSMKH
NT Sequence
ATGATTGTCG TCGTCGTCGA TTCGATGGAG CGGTACTACT TTGCCGTGCG GCTCGTGAAG GCGGTCAGAA AGGAATTCGA CTTTCTGTTC GCCGCGAGCG AGCCGCTCGC GCACCTGATG GCACTGGCCG CGGGCTTTCG GTCGGTCTAT CTGCGGCGCG GGGCGCATGC GCCCGCCATG CTCGATGCGG CGGCCGGGAT TCGGTCCGAT GCATCGATCG AGGTGCTGAA CGGACAGATG ACGCCCGAGC GCGCGCGCGC CGACGCGCAG GCGGTCTTCG CCGCGATGTC CGGCGTGTTC CGCCGTCATC TCGTGTCGCA GTGCCTGATG TGGAACGGCC AGCAGCTCGT TTGCCGCGCG GTCGCGCACG CGTGCGCGGC GCACGGGGTA CCGACGAAAT TCGTCGAGAT CTCGAATTTG CCGGACAAGC TGTTCGTCGA TCGGCTCGGC GTCAACGCGC TGTCGTCGAT CAGCCGCAAT CCGGCCGTCA TCGACGGCTT GCCGATGCCG ACCGAAGGCG AGCATCGCCG CTGGTTCGCG CGCTACGAGG CGTACAAGGC GCGGCCGCTG CCGCAGTCGC GCACGTCGTG GGCGCGCAAG GCGATGTCGG CCGCGAACTA TGCGCTGAAG CTCGCCACGC AAGGCGTGGC GCGCAAACGT TTGGACGCGG CGCGCGCGAC GAACGGCGCG CGCGCGCCGG CGCAGGCGAA AGTGCTGAGC ACGAAGGAAC TCTCGGCGCT GCGCTACGTG TTCCTGCCGC TGCAGGTGTC GGGCGACACG CAGATCAAGC TGCATTCGGA TGTCGACAAC CTGAAGGCGA TCCGGCTCGC GTTCGAGCAC GCGGCGAACG AGAACGCGGA CCTGATCGTC AAGCTGCATC CGGCCGAGCG TGACGTGGCC GTGATCGACG AGGTGGTGCG GATGCAGCGC GTCTATCACT TCGACCTCGT GACGTCGCCG ACCACCGATC TGATCAAGCA CGCGCATTCG GTCGTCACGA TCAATTCGAC GGTCGGCCTC GAGGCGCTGC TGTACGGCAA GCCGGTCGTG TCGCTCGGCC GGTGCTTCTA CAAGGAGTTC GATCGCGCGA GGCTGCTCAA GTACATCCAT GCGTTCCTGA TCGACGGCAT CGATTACTTC GGTCGAGCGG ACATCGCGCC GCGCGCCGCG CGAAACGTGT TCTCGATGAA GCAC
Details for BupsA.17338.b.A1.GE31164
HARVESTED ON: 12/21/2010
SEQUENCED ON: 12/27/2010
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 61
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIVVVVDSM ERYYFAVRLV KAVRKEFDFL FAASEPLAHL MALAAGFRSV YLRRGAHAPA MLDAAAGIRS DASIEVLNGQ MTPERARADA QAVFAAMSGV FRRHLVSQCL MWNGQQLVCR AVAHACAAHG VPTKFVEISN LPDKLFVDRL GVNALSSISR NPAVIDGLPM PTEGEHRRWF ARYEAYKARP LPQSRTSWAR KAMSAANYAL KLATQGVARK RLDAARATNA RARXRXRKC
Validated NT Sequence
annnnnnnnn nnnnancttt aagaaggaga tataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgattgtcgt cgtcgtcgat tcgatggagc ggtactactt tgccgtgcgg ctcgtgaagg cggtcagaaa ggaattcgac tttctgttcg ccgcgagcga gccgctcgcg cacctgatgg cactggccgc gggctttcgg tcggtctatc tgcggcgcgg ggcgcatgcg cccgccatgc tcgatgcggc ggccgggatt cggtccgatg catcgatcga ggtgctgaac ggacagatga cgcccgagcg cgcgcgcgcc gacgcgcagg cggtcttcgc cgcgatgtcc ggcgtgttcc gccgtcatct cgtgtcgcag tgcctgatgt ggaacggcca gcagctcgtt tgccgcgcgg tcgcgcacgc gtgcgcggcg cacggggtac cgacgaaatt cgtcgagatc tcgaatttgc cggacaagct gttcgtcgat cggctcggcg tcaacgcgct gtcgtcgatc agccgcaatc cggccgtcat cgacggcttg ccgatgccga ccgaaggcga gcatcgccgc tggttcgcgc gctacgaggc gtacaaggcg cggccgctgc cgcagtcgcg cacgtcgtgg gcgcgcaagg cgatgtcggc cgcgaactat gcgctgaagc tcgccacgca aggcgtggcg cgcaaacgtt tggacgcggc gcgcgcgacg aacgcgcgcg cgcgccnncg cangcgaaag tgctgagcac gaaggaactc tcggcgctgc gctacgngnn nntgcngcng nnnnngtcng gcgacncgcc nagctgcatt cnnatgncga cnnnntgaag gcnntncngn ntcgcgttcn agcacnnngn cnnnnannnn nngannnatc ntnnnntncn tnnnnnnacg tgacnnnnnn gatcgacnan nnnngnnnnn ncannncntn tnnnnntnnn nnnnnnnncg nnnnnnnnnc nnntnngatn nnnnnnnnnn nnnnngcnnn nngnnnnnnn nnnnnnnnng nnnnnnnnnn nnnnnnnnnn gnnnnnnnnn nnnntng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIVVVVDSM ERYYFAVRLV KAVRKEFDFL FAASEPLAHL MALAAGFRSV YLRRGAHAPA MLDAAAGIRS DASIEVLNGQ MTPERARADA QAVFAAMSGV FRRHLVSQCL MWNGQQLVCR AVAHACAAHG VPTKFVEISN LPDKLFVDRL GVNALSSISR NPAVIDGLPM PTEGEHRRWF ARYEAYKARP LPQSRTSWAR KAMSAANYAL KLATQGVARK RLDAARATNG ARAPAQAKVL STKELSALRY VFLPLQVSGD TQIKLHSDVD NLKAIRLAFE HAANENADLI VKLHPAERDV AVIDEVVRMQ RVYHFDLVTS PTTDLIKHAH SVVTINSTVG LEALLYGKPV VSLGRCFYKE FDRARLLKYI HAFLIDGIDY FGRADIAPRA ARNVFSMKH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattgtcgtc gtcgtcgatt cgatggagcg gtactacttt gccgtgcggc tcgtgaaggc ggtcagaaag gaattcgact ttctgttcgc cgcgagcgag ccgctcgcgc acctgatggc actggccgcg ggctttcggt cggtctatct gcggcgcggg gcgcatgcgc ccgccatgct cgatgcggcg gccgggattc ggtccgatgc atcgatcgag gtgctgaacg gacagatgac gcccgagcgc gcgcgcgccg acgcgcaggc ggtcttcgcc gcgatgtccg gcgtgttccg ccgtcatctc gtgtcgcagt gcctgatgtg gaacggccag cagctcgttt gccgcgcggt cgcgcacgcg tgcgcggcgc acggggtacc gacgaaattc gtcgagatct cgaatttgcc ggacaagctg ttcgtcgatc ggctcggcgt caacgcgctg tcgtcgatca gccgcaatcc ggccgtcatc gacggcttgc cgatgccgac cgaaggcgag catcgccgct ggttcgcgcg ctacgaggcg tacaaggcgc ggccgctgcc gcagtcgcgc acgtcgtggg cgcgcaaggc gatgtcggcc gcgaactatg cgctgaagct cgccacgcaa ggcgtggcgc gcaaacgttt ggacgcggcg cgcgcgacga acggcgcgcg cgcgccggcg caggcgaaag tgctgagcac gaaggaactc tcggcgctgc gctacgtgtt cctgccgctg caggtgtcgg gcgacacgca gatcaagctg cattcggatg tcgacaacct gaaggcgatc cggctcgcgt tcgagcacgc ggcgaacgag aacgcggacc tgatcgtcaa gctgcatccg gccgagcgtg acgtggccgt gatcgacgag gtggtgcgga tgcagcgcgt ctatcacttc gacctcgtga cgtcgccgac caccgatctg atcaagcacg cgcattcggt cgtcacgatc aattcgacgg tcggcctcga ggcgctgctg tacggcaagc cggtcgtgtc gctcggccgg tgcttctaca aggagttcga tcgcgcgagg ctgctcaagt acatccatgc gttcctgatc gacggcatcg attacttcgg tcgagcggac atcgcgccgc gcgccgcgcg aaacgtgttc tcgatgaagc actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa