BupsA.17337.a

3-deoxy-manno-octulosonate-8-phosphatase

CENTER ID: BupsA.17337.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17337.a.A1.GE31161 Full length( BupsA.17337.a ) 1 183
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17337.a.A1.PS01007 Full length( BupsA.17337.a ) 1 183

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3400
RefSeq: YP_334634.1
UniProt: Q3JP69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAPAYRGAI RLVLFDVDGV LTDGRLHVTG DGECMKSFHA KDGIAVALLH AHGIRSGILS GRHSEALAWR ARQLGFDVCV SGCGDKRAGY ARIKSEQALA DHAIAYVGDD VNDLPVIDAV GVSYAPADAH VLVRRRVDYV VSRAGGEGAA REVAEHVLLR SGLSLDDAYR PLLDEWARHA VVQ
NT Sequence
ATGAGCGCGC CGGCGTATCG CGGCGCGATT CGGCTCGTGC TGTTCGACGT CGACGGCGTG CTGACCGACG GGCGGCTGCA TGTGACGGGC GACGGAGAGT GCATGAAGAG CTTCCATGCG AAGGACGGCA TCGCCGTTGC GCTGCTGCAC GCGCACGGCA TTCGCAGCGG CATTCTGTCG GGCCGCCACA GCGAGGCGCT CGCATGGCGC GCGCGGCAGC TCGGTTTCGA CGTGTGCGTG TCGGGCTGCG GCGACAAGCG CGCCGGCTAC GCGCGCATCA AGTCCGAACA GGCGCTCGCG GACCATGCGA TCGCGTATGT CGGCGACGAC GTCAACGATC TGCCCGTGAT CGACGCGGTC GGCGTCTCGT ATGCGCCCGC CGACGCGCAC GTGCTCGTCA GGCGGCGCGT CGACTACGTC GTCTCGCGCG CGGGCGGCGA GGGCGCCGCG CGGGAGGTCG CCGAGCACGT GCTGTTGCGC TCGGGGTTGT CGCTCGACGA CGCATATCGG CCGTTGCTCG ACGAATGGGC TCGGCATGCC GTCGTCCAA
Details for BupsA.17337.a.A1.GE31161
HARVESTED ON: 12/21/2010
SEQUENCED ON: 12/27/2010
EXPECTED MW: 21kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPAYRGA IRLVLFDVDG VLTDGRLHVT GDGECMKSFH AKDGIAVALL HAHGIRSGIL SGRHSEALAW RARQLGFDVC VSGCGDKRAG YARIKSEQAL ADHAIAYVGD DVNDLPVIDA VGVSYAPADA HVLVRRRVDY VVSRAGGEGA AREVAEHVLL RSGLSLDDAY RPLLDEWARH AVVQ
Validated NT Sequence
ttattattgg acgacggcat gccgagccca ttcgtcgagc aacggccgat atgcgtcgtc gagcgacaac cccgagcgca acagcacgtg ctcggcgacc tcccgcgcgg cgccctcgcc gcccgcgcgc gagacgacgt agtcgacgcg ccgcctgacg agcacgtgcg cgtcggcggg cgcatacgag acgccgaccg cgtcgatcac gggcagatcg ttgacgtcgt cgccgacata cgcgatcgca tggtccgcga gcgcctgttc ggacttgatg cgcgcgtagc cggcgcgctt gtcgccgcag cccgacacgc acacgtcgaa accgagctgc cgcgcgcgcc atgcgagcgc ctcgctgtgg cggcccgaca gaatgccgct gcgaatgccg tgcgcgtgca gcagcgcaac ggcgatgccg tccttcgcat ggaagctctt catgcactct ccgtcgcccg tcacatgcag ccgcccgtcg gtcagcacgc cgtcgacgtc gaacagcacg agccgaatcg cgccgcgata cgccggcgcg ctcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPAYRGA IRLVLFDVDG VLTDGRLHVT GDGECMKSFH AKDGIAVALL HAHGIRSGIL SGRHSEALAW RARQLGFDVC VSGCGDKRAG YARIKSEQAL ADHAIAYVGD DVNDLPVIDA VGVSYAPADA HVLVRRRVDY VVSRAGGEGA AREVAEHVLL RSGLSLDDAY RPLLDEWARH AVVQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcgccg gcgtatcgcg gcgcgattcg gctcgtgctg ttcgacgtcg acggcgtgct gaccgacggg cggctgcatg tgacgggcga cggagagtgc atgaagagct tccatgcgaa ggacggcatc gccgttgcgc tgctgcacgc gcacggcatt cgcagcggca ttctgtcggg ccgccacagc gaggcgctcg catggcgcgc gcggcagctc ggtttcgacg tgtgcgtgtc gggctgcggc gacaagcgcg ccggctacgc gcgcatcaag tccgaacagg cgctcgcgga ccatgcgatc gcgtatgtcg gcgacgacgt caacgatctg cccgtgatcg acgcggtcgg cgtctcgtat gcgcccgccg acgcgcacgt gctcgtcagg cggcgcgtcg actacgtcgt ctcgcgcgcg ggcggcgagg gcgccgcgcg ggaggtcgcc gagcacgtgc tgttgcgctc ggggttgtcg ctcgacgacg catatcggcc gttgctcgac gaatgggctc ggcatgccgt cgtccaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BupsA.17337.a.A1.PS01007
PURIFICATION DATe: 3/18/2011
CONCENTRATION: 10.54mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPAYRGA IRLVLFDVDG VLTDGRLHVT GDGECMKSFH AKDGIAVALL HAHGIRSGIL SGRHSEALAW RARQLGFDVC VSGCGDKRAG YARIKSEQAL ADHAIAYVGD DVNDLPVIDA VGVSYAPADA HVLVRRRVDY VVSRAGGEGA AREVAEHVLL RSGLSLDDAY RPLLDEWARH AVVQ
Validated NT Sequence
ttattattgg acgacggcat gccgagccca ttcgtcgagc aacggccgat atgcgtcgtc gagcgacaac cccgagcgca acagcacgtg ctcggcgacc tcccgcgcgg cgccctcgcc gcccgcgcgc gagacgacgt agtcgacgcg ccgcctgacg agcacgtgcg cgtcggcggg cgcatacgag acgccgaccg cgtcgatcac gggcagatcg ttgacgtcgt cgccgacata cgcgatcgca tggtccgcga gcgcctgttc ggacttgatg cgcgcgtagc cggcgcgctt gtcgccgcag cccgacacgc acacgtcgaa accgagctgc cgcgcgcgcc atgcgagcgc ctcgctgtgg cggcccgaca gaatgccgct gcgaatgccg tgcgcgtgca gcagcgcaac ggcgatgccg tccttcgcat ggaagctctt catgcactct ccgtcgcccg tcacatgcag ccgcccgtcg gtcagcacgc cgtcgacgtc gaacagcacg agccgaatcg cgccgcgata cgccggcgcg ctcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPAYRGA IRLVLFDVDG VLTDGRLHVT GDGECMKSFH AKDGIAVALL HAHGIRSGIL SGRHSEALAW RARQLGFDVC VSGCGDKRAG YARIKSEQAL ADHAIAYVGD DVNDLPVIDA VGVSYAPADA HVLVRRRVDY VVSRAGGEGA AREVAEHVLL RSGLSLDDAY RPLLDEWARH AVVQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGCGCCG GCGTATCGCG GCGCGATTCG GCTCGTGCTG TTCGACGTCG ACGGCGTGCT GACCGACGGG CGGCTGCATG TGACGGGCGA CGGAGAGTGC ATGAAGAGCT TCCATGCGAA GGACGGCATC GCCGTTGCGC TGCTGCACGC GCACGGCATT CGCAGCGGCA TTCTGTCGGG CCGCCACAGC GAGGCGCTCG CATGGCGCGC GCGGCAGCTC GGTTTCGACG TGTGCGTGTC GGGCTGCGGC GACAAGCGCG CCGGCTACGC GCGCATCAAG TCCGAACAGG CGCTCGCGGA CCATGCGATC GCGTATGTCG GCGACGACGT CAACGATCTG CCCGTGATCG ACGCGGTCGG CGTCTCGTAT GCGCCCGCCG ACGCGCACGT GCTCGTCAGG CGGCGCGTCG ACTACGTCGT CTCGCGCGCG GGCGGCGAGG GCGCCGCGCG GGAGGTCGCC GAGCACGTGC TGTTGCGCTC GGGGTTGTCG CTCGACGACG CATATCGGCC GTTGCTCGAC GAATGGGCTC GGCATGCCGT CGTCCAATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA