SSGCID: Target Page

BupsA.17335.b

Putative kinase

CENTER ID:	BupsA.17335.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17335.b.A1.GE31158	Full length( BupsA.17335.b )		1	329

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.2239
RefSeq:	YP_333549.1
UniProt:	Q3JSA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRPGARRAA RAASLDAGRA SSRPMTARAL AMSEPMRSLI PAARTASGIS YCSFGELLQG VLLEDDADFL VTLPIQKYSV STFVPDPGST EIVTQPSGKT KAAALAKVIL GRYGHNVGGT FYIDCDIPIG KGLSSSSADL LATARAIEVY MGRELPLGEL CRDMSGIEPT DGVMFAESVV YLQRKGVLWS RLGRLSGIQI LSLDEGGTID TLEYHRRARA HRHNAEHRSE FNELLERIVA AFGTRDLDEI GRVSTRSAYI NQKVNPKRHL ASVHDVCQAT RGLGLVTAHS GTCIGILYET GRAGHRENLA RAADALSGYG NIKVYDTIQ

NT Sequence

GTGCGGCGAC CAGGCGCTCG GCGAGCTGCT CGCGCAGCTT CGCTCGATGC TGGACGCGCA TCGTCCCGGC CGATGACCGC TCGAGCACTG GCCATGTCGG AGCCCATGCG TTCACTCATC CCGGCCGCGC GAACGGCGTC GGGCATCTCG TACTGCTCGT TCGGCGAACT GCTGCAGGGC GTGCTGCTCG AGGACGACGC CGATTTTCTC GTCACGCTGC CGATCCAGAA ATACTCGGTG AGCACGTTCG TGCCGGACCC CGGCAGCACC GAGATCGTCA CGCAGCCGAG CGGCAAGACG AAGGCCGCGG CGCTCGCGAA GGTGATTCTC GGGCGCTACG GGCACAACGT CGGCGGGACG TTCTACATCG ACTGCGACAT CCCGATCGGC AAGGGGCTCA GCAGCTCGTC CGCCGACCTG CTCGCGACCG CGCGCGCGAT CGAGGTCTAC ATGGGCCGCG AGCTGCCGCT CGGCGAGCTG TGCCGCGACA TGAGCGGCAT CGAGCCGACC GACGGCGTGA TGTTCGCGGA ATCGGTCGTC TACCTGCAGC GCAAGGGGGT GCTGTGGTCG CGGCTCGGGC GCCTGTCCGG CATCCAGATC CTGTCGCTCG ACGAGGGCGG CACGATCGAC ACGCTCGAGT ATCACCGCCG CGCGCGCGCG CATCGGCACA ACGCCGAGCA TCGCAGCGAG TTCAACGAAC TGCTCGAGCG GATCGTCGCG GCGTTCGGCA CGCGCGATCT CGACGAGATC GGCAGGGTGT CGACCCGCAG CGCGTACATC AACCAGAAGG TCAATCCGAA GCGCCATCTG GCCTCGGTTC ACGACGTATG CCAGGCGACG CGGGGGCTCG GGCTCGTGAC GGCGCACAGC GGCACGTGCA TCGGCATCCT GTACGAAACC GGGCGGGCGG GGCACCGCGA AAACCTTGCG CGGGCGGCCG ATGCGCTCTC GGGATACGGA AACATCAAGG TCTATGACAC GATCCAA

Details for BupsA.17335.b.A1.GE31158

HARVESTED ON:	12/21/2010
SEQUENCED ON:	12/27/2010
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	84
PERCENT COVERAGE:	104

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVRRPGARR AARAASLDAG RASSRPMTAR ALAMSEPMRS LIPAARTASG ISYCSFGELL QGVLLEDDAD FLVTLPIQKY SVSTFVPDPG STEIVTQPSG KTKAAALAKV ILGRYGHNVG GTFYIDCDIP IGKGLSSSSA DLLATARAIE VYMGRELPLG ELCRDMSGIE PTDGVMFAES VVYLQRKGVL WSRLGRLSGI QILSLDEGGT IDTLEYHRRA RAHRHNAEHR SEFNELLERI VAAFGTRDLD XIGRVSTRSA YXNQKVNPKR HLXLGXRRMP XDAGARARXG AXXHVHXHPX RXXAGGAXXK XXGXXXXLSX XXTXXXXXDX XXXXXXXXXX XXXXXXXX

Validated NT Sequence

annnttnnnn nnntacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcggcga ccaggcgctc ggcgagctgc tcgcgcagct tcgctcgatg ctggacgcgc atcgtcccgg ccgatgaccg ctcgagcact ggccatgtcg gagcccatgc gttcactcat cccggccgcg cgaacggcgt cgggcatctc gtactgctcg ttcggcgaac tgctgcaggg cgtgctgctc gaggacgacg ccgattttct cgtcacgctg ccgatccaga aatactcggt gagcacgttc gtgccggacc ccggcagcac cgagatcgtc acgcagccga gcggcaagac gaaggccgcg gcgctcgcga aggtgattct cgggcgctac gggcacaacg tcggcgggac gttctacatc gactgcgaca tcccgatcgg caaggggctc agcagctcgt ccgccgacct gctcgcgacc gcgcgcgcga tcgaggtcta catgggccgc gagctgccgc tcggcgagct gtgccgcgac atgagcggca tcgagccgac cgacggcgtg atgttcgcgg aatcggtcgt ctacctgcag cgcaaggggg tgctgtggtc gcggctcggg cgcctgtccg gcatccagat cctgtcgctc gacgagggcg gcacgatcga cacgctcgag tatcaccgcc gcgcgcgcgc gcatcggcac aacgccgagc atcgcagcga gttcaacgaa ctgctcgagc ggatcgtcgc ggcgttcggc acgcgcgatc tcgacganat cggcagggtg tcnacccgca gcgcgtacnt caaccagaag gtcaatccga agcgccatct ggncctcggn ncacgacgta tgccangnga cgcgggggct cgggctcgtn anggcgcaca nnngcacgtg catcngcatc ctgnacgaan nngggcgggn ggggcancnn naaaancntn cgggnngncn nanncnctct cnnnnannna aacatnnnnn nnnnnanncg atnnnnnant nannngnnnn nnnnnnnnnn nnnnnnnntn nnnnnnnnnn nnnnnnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVRRPGARR AARAASLDAG RASSRPMTAR ALAMSEPMRS LIPAARTASG ISYCSFGELL QGVLLEDDAD FLVTLPIQKY SVSTFVPDPG STEIVTQPSG KTKAAALAKV ILGRYGHNVG GTFYIDCDIP IGKGLSSSSA DLLATARAIE VYMGRELPLG ELCRDMSGIE PTDGVMFAES VVYLQRKGVL WSRLGRLSGI QILSLDEGGT IDTLEYHRRA RAHRHNAEHR SEFNELLERI VAAFGTRDLD EIGRVSTRSA YINQKVNPKR HLASVHDVCQ ATRGLGLVTA HSGTCIGILY ETGRAGHREN LARAADALSG YGNIKVYDTI Q

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcggcga ccaggcgctc ggcgagctgc tcgcgcagct tcgctcgatg ctggacgcgc atcgtcccgg ccgatgaccg ctcgagcact ggccatgtcg gagcccatgc gttcactcat cccggccgcg cgaacggcgt cgggcatctc gtactgctcg ttcggcgaac tgctgcaggg cgtgctgctc gaggacgacg ccgattttct cgtcacgctg ccgatccaga aatactcggt gagcacgttc gtgccggacc ccggcagcac cgagatcgtc acgcagccga gcggcaagac gaaggccgcg gcgctcgcga aggtgattct cgggcgctac gggcacaacg tcggcgggac gttctacatc gactgcgaca tcccgatcgg caaggggctc agcagctcgt ccgccgacct gctcgcgacc gcgcgcgcga tcgaggtcta catgggccgc gagctgccgc tcggcgagct gtgccgcgac atgagcggca tcgagccgac cgacggcgtg atgttcgcgg aatcggtcgt ctacctgcag cgcaaggggg tgctgtggtc gcggctcggg cgcctgtccg gcatccagat cctgtcgctc gacgagggcg gcacgatcga cacgctcgag tatcaccgcc gcgcgcgcgc gcatcggcac aacgccgagc atcgcagcga gttcaacgaa ctgctcgagc ggatcgtcgc ggcgttcggc acgcgcgatc tcgacgagat cggcagggtg tcgacccgca gcgcgtacat caaccagaag gtcaatccga agcgccatct ggcctcggtt cacgacgtat gccaggcgac gcgggggctc gggctcgtga cggcgcacag cggcacgtgc atcggcatcc tgtacgaaac cgggcgggcg gggcaccgcg aaaaccttgc gcgggcggcc gatgcgctct cgggatacgg aaacatcaag gtctatgaca cgatccaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a