BupsA.17333.a

Aminotransferase, DegT/DnrJ/EryC1/StrS family

CENTER ID: BupsA.17333.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17333.a.A1.GE31153 Full length( BupsA.17333.a ) 1 383
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2510
RefSeq: YP_333796.1
UniProt: Q3JRK7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQTSVPFLP FVKPEIDEET IQGVADVLRS GWITTGPQCQ RFEAALSEYC GGRPVRVFNS GTATLEIGLR IAGVGPGDEV ITTPASWVAT SNVILETGAT PVFVDIDPVT RNLDLDLVEK AITPRTKAII PVYLAGLPVD MDRLYAIARA HELRVIEDAA QALGSTWRGQ RIGAIGDIVS FSFHPNKNIT SIEGGALVLN DEAEATLAQK YRLQGITRTG LDGMDCDVLG GKYNLTDVAA RVGWGQLAHL ERFNATRHAL ARRYFDAFAN GPACALGVGL PVADFENTNW HMFQITLPLE KLSISRGEFM EQLKQRGIGS GVHYPAIHLF TLYRARGFAQ GMFPHAERFG ASTVTLPLFT QMSEAHVQRV VDAVNQICEQ YGK
NT Sequence
ATGAGCCAGA CTTCCGTTCC TTTCCTGCCG TTCGTCAAAC CCGAGATCGA CGAGGAAACC ATTCAGGGCG TGGCCGACGT GCTGCGCTCC GGCTGGATCA CGACCGGCCC GCAGTGCCAG CGCTTCGAGG CCGCGCTGTC CGAATACTGC GGCGGGCGTC CCGTACGCGT GTTCAACTCG GGCACGGCGA CGCTCGAGAT CGGCCTGCGG ATCGCGGGCG TCGGCCCCGG CGACGAAGTG ATCACGACGC CCGCGTCGTG GGTCGCGACG AGCAACGTGA TCCTCGAGAC GGGCGCGACG CCCGTGTTCG TCGACATCGA TCCCGTCACG CGCAACCTCG ATCTCGACCT CGTCGAAAAG GCGATCACGC CGCGCACGAA GGCGATCATC CCGGTGTACC TCGCGGGCCT GCCCGTCGAC ATGGACCGCC TCTACGCGAT CGCGCGCGCG CACGAGTTGC GCGTGATCGA GGACGCCGCG CAGGCGCTCG GCTCGACGTG GCGCGGCCAG CGCATCGGCG CGATCGGCGA CATCGTGTCG TTCAGCTTCC ATCCGAACAA GAACATCACG TCGATCGAAG GCGGCGCGCT CGTGCTCAAC GACGAGGCCG AGGCGACGCT CGCGCAGAAG TACCGGCTGC AGGGCATCAC GCGCACGGGC CTCGACGGGA TGGACTGCGA CGTGCTCGGC GGCAAGTACA ATCTGACCGA CGTCGCCGCG CGCGTCGGCT GGGGCCAGCT CGCGCACCTC GAACGCTTCA ACGCGACGCG GCACGCGCTC GCGCGCCGCT ACTTCGACGC GTTCGCGAAC GGGCCCGCGT GCGCGCTCGG CGTCGGGCTG CCCGTCGCGG ATTTCGAAAA CACCAACTGG CACATGTTCC AGATCACGCT GCCGCTCGAG AAGCTGTCGA TCTCGCGCGG CGAATTCATG GAGCAGTTGA AGCAGCGCGG CATCGGCTCG GGCGTCCATT ACCCGGCCAT TCATCTTTTC ACGCTGTACC GCGCGCGCGG CTTTGCGCAG GGCATGTTCC CCCACGCCGA GCGCTTCGGC GCGTCGACCG TCACGCTGCC GCTCTTCACG CAGATGAGCG AGGCGCACGT GCAACGGGTC GTCGACGCCG TCAACCAGAT TTGCGAACAA TACGGAAAA
Details for BupsA.17333.a.A1.GE31153
HARVESTED ON: 12/21/2010
SEQUENCED ON: 12/27/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMSQTSVP FLPFVKPEID EETIQGVADV LRSGWITTGP QCQRFEAALS EYCGGRPVRV FNSGTATLEI GLRIAGVGPG DEVITTPASW VATSNVILET GATPVFVDID PVTRNLDLDL VEKAITPRTK AIIPVYLAGL PVDMDRLYAI ARAHELRVIE DAAQALGSTW RGQRIGAIGD IVSFSFHPNK NITSIEGGAL VLNDEAEATL AQKYRLQGIT RTGLDGMDCD VLGGKYNLTD VAARVGWGQL AHLERFNATR HALARRYFDA FANGPACALG VGLPVADFEN TNWHMFQITL PLEKLSISRG EFMEQLKQRG IGSGVHYPAI HLFTLYRARG FAQGMFPHAE RFGASTVTLP LFTQMSEAHV QRVVDAVNQI CEQYGK
Validated NT Sequence
ttattatttt ccgtattgtt cgcaaatctg gttgacggcg tcgacgaccc gttgcacgtg cgcctcgctc atctgcgtga agagcggcag cgtgacggtc gacgcgccga agcgctcggc gtgggggaac atgccctgcg caaagccgcg cgcgcggtac agcgtgaaaa gatgaatggc cgggtaatgg acgcccgagc cgatgccgcg ctgcttcaac tgctccatga attcgccgcg cgagatcgac agcttctcga gcggcagcgt gatctggaac atgtgccagt tggtgttttc gaaatccgcg acgggcagcc cgacgccgag cgcgcacgcg ggcccgttcg cgaacgcgtc gaagtagcgg cgcgcgagcg cgtgccgcgt cgcgttgaag cgttcgaggt gcgcgagctg gccccagccg acgcgcgcgg cgacgtcggt cagattgtac ttgccgccga gcacgtcgca gtccatcccg tcgaggcccg tgcgcgtgat gccctgcagc cggtacttct gcgcgagcgt cgcctcggcc tcgtcgttga gcacgagcgc gccgccttcg atcgacgtga tgttcttgtt cggatggaag ctgaacgaca cgatgtcgcc gatcgcgccg atgcgctggc cgcgccacgt cgagccgagc gcctgcgcgg cgtcctcgat cacgcgcaac tcgtgcgcgc gcgcgatcgc gtagaggcgg tccatgtcga cgggcaggcc cgcgaggtac accgggatga tcgccttcgt gcgcggcgtg atcgcctttt cgacgaggtc gagatcgagg ttgcgcgtga cgggatcgat gtcgacgaac acgggcgtcg cgcccgtctc gaggatcacg ttgctcgtcg cgacccacga cgcgggcgtc gtgatcactt cgtcgccggg gccgacgccc gcgatccgca ggccgatctc gagcgtcgcc gtgcccgagt tgaacacgcg tacgggacgc ccgccgcagt attcggacag cgcggcctcg aagcgctggc actgcgggcc ggtcgtgatc cagccggagc gcagcacgtc ggccacgccc tgaatggttt cctcgtcgat ctcgggtttg acgaacggca ggaaaggaac ggaagtctgg ctcatcgaac caggaccctg ggtctgagct tccagggtac ccata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQTSVPFL PFVKPEIDEE TIQGVADVLR SGWITTGPQC QRFEAALSEY CGGRPVRVFN SGTATLEIGL RIAGVGPGDE VITTPASWVA TSNVILETGA TPVFVDIDPV TRNLDLDLVE KAITPRTKAI IPVYLAGLPV DMDRLYAIAR AHELRVIEDA AQALGSTWRG QRIGAIGDIV SFSFHPNKNI TSIEGGALVL NDEAEATLAQ KYRLQGITRT GLDGMDCDVL GGKYNLTDVA ARVGWGQLAH LERFNATRHA LARRYFDAFA NGPACALGVG LPVADFENTN WHMFQITLPL EKLSISRGEF MEQLKQRGIG SGVHYPAIHL FTLYRARGFA QGMFPHAERF GASTVTLPLF TQMSEAHVQR VVDAVNQICE QYGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagccagact tccgttcctt tcctgccgtt cgtcaaaccc gagatcgacg aggaaaccat tcagggcgtg gccgacgtgc tgcgctccgg ctggatcacg accggcccgc agtgccagcg cttcgaggcc gcgctgtccg aatactgcgg cgggcgtccc gtacgcgtgt tcaactcggg cacggcgacg ctcgagatcg gcctgcggat cgcgggcgtc ggccccggcg acgaagtgat cacgacgccc gcgtcgtggg tcgcgacgag caacgtgatc ctcgagacgg gcgcgacgcc cgtgttcgtc gacatcgatc ccgtcacgcg caacctcgat ctcgacctcg tcgaaaaggc gatcacgccg cgcacgaagg cgatcatccc ggtgtacctc gcgggcctgc ccgtcgacat ggaccgcctc tacgcgatcg cgcgcgcgca cgagttgcgc gtgatcgagg acgccgcgca ggcgctcggc tcgacgtggc gcggccagcg catcggcgcg atcggcgaca tcgtgtcgtt cagcttccat ccgaacaaga acatcacgtc gatcgaaggc ggcgcgctcg tgctcaacga cgaggccgag gcgacgctcg cgcagaagta ccggctgcag ggcatcacgc gcacgggcct cgacgggatg gactgcgacg tgctcggcgg caagtacaat ctgaccgacg tcgccgcgcg cgtcggctgg ggccagctcg cgcacctcga acgcttcaac gcgacgcggc acgcgctcgc gcgccgctac ttcgacgcgt tcgcgaacgg gcccgcgtgc gcgctcggcg tcgggctgcc cgtcgcggat ttcgaaaaca ccaactggca catgttccag atcacgctgc cgctcgagaa gctgtcgatc tcgcgcggcg aattcatgga gcagttgaag cagcgcggca tcggctcggg cgtccattac ccggccattc atcttttcac gctgtaccgc gcgcgcggct ttgcgcaggg catgttcccc cacgccgagc gcttcggcgc gtcgaccgtc acgctgccgc tcttcacgca gatgagcgag gcgcacgtgc aacgggtcgt cgacgccgtc aaccagattt gcgaacaata cggaaaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa