BupsA.17331.a

GntS

CENTER ID: BupsA.17331.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17331.a.A1.GE31151 Full length( BupsA.17331.a ) 1 210
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17331.a.A1.PS01030 Full length( BupsA.17331.a ) 1 210

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.359
RefSeq: YP_331771.1
UniProt: Q3JXD2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYTVKEIFY TLQGEGANAG RPAVFCRFAG CNLWTGREAD RDGAVCRFCD TDFVGTDGEN GGKFKDAASL AARIASLWPA GEAYRFVVCT GGEPMLQLDQ PLVDALHAAG FTIAIETNGS LPVLESIDWI CVSPKADAPL VVTKGNELKV VVPQDNQRLA DYAKLDFEHF LVQPMDGPSR ELNTRLAIDW CKRHPRWRLS MQTHKYLNIP
NT Sequence
ATGACGTACA CGGTCAAGGA AATTTTCTAT ACGTTGCAGG GCGAGGGCGC GAACGCCGGC CGTCCGGCCG TGTTCTGCCG GTTCGCCGGC TGCAACCTCT GGACGGGGCG CGAAGCGGAC CGCGACGGCG CGGTGTGCCG CTTCTGCGAT ACCGACTTCG TCGGCACCGA CGGCGAGAAC GGCGGCAAGT TCAAGGATGC CGCTTCGCTC GCGGCGAGGA TCGCGTCGCT CTGGCCGGCG GGCGAGGCGT ATCGCTTCGT CGTCTGCACG GGCGGCGAGC CGATGCTGCA GCTCGATCAG CCGCTCGTCG ACGCGCTGCA CGCGGCGGGC TTCACGATCG CGATCGAGAC GAACGGCTCG CTGCCCGTGC TCGAATCGAT CGACTGGATC TGCGTGAGCC CGAAGGCGGA TGCGCCGCTC GTCGTCACGA AGGGCAACGA ACTGAAGGTG GTGGTGCCGC AGGACAACCA GCGCCTTGCC GATTACGCGA AGCTCGACTT CGAGCATTTC CTCGTGCAGC CGATGGACGG CCCGTCGCGC GAGCTCAACA CCCGGCTCGC GATCGATTGG TGCAAGCGTC ATCCGCGGTG GCGGCTGTCG ATGCAGACGC ACAAGTACCT GAACATTCCC
Details for BupsA.17331.a.A1.GE31151
HARVESTED ON: 12/21/2010
SEQUENCED ON: 12/27/2010
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTYTVKEIF YTLQGEGANA GRPAVFCRFA GCNLWTGREA DRDGAVCRFC DTDFVGTDGE NGGKFKDAAS LAARIASLWP AGEAYRFVVC TGGEPMLQLD QPLVDALHAA GFTIAIETNG SLPVLESIDW ICVSPKADAP LVVTKGNELK VVVPQDNQRL ADYAKLDFEH FLVQPMDGPS RELNTRLAID WCKRHPRWRL SMQTHKYLNI P
Validated NT Sequence
ttattaggga atgttcaggt acttgtgcgt ctgcatcgac agccgccacc gcggatgacg cttgcaccaa tcgatcgcga gccgggtgtt gagctcgcgc gacgggccgt ccatcggctg cacgaggaaa tgctcgaagt cgagcttcgc gtaatcggca aggcgctggt tgtcctgcgg caccaccacc ttcagttcgt tgcccttcgt gacgacgagc ggcgcatccg ccttcgggct cacgcagatc cagtcgatcg attcgagcac gggcagcgag ccgttcgtct cgatcgcgat cgtgaagccc gccgcgtgca gcgcgtcgac gagcggctga tcgagctgca gcatcggctc gccgcccgtg cagacgacga agcgatacgc ctcgcccgcc ggccagagcg acgcgatcct cgccgcgagc gaagcggcat ccttgaactt gccgccgttc tcgccgtcgg tgccgacgaa gtcggtatcg cagaagcggc acaccgcgcc gtcgcggtcc gcttcgcgcc ccgtccagag gttgcagccg gcgaaccggc agaacacggc cggacggccg gcgttcgcgc cctcgccctg caacgtatag aaaatttcct tgaccgtgta cgtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYTVKEIF YTLQGEGANA GRPAVFCRFA GCNLWTGREA DRDGAVCRFC DTDFVGTDGE NGGKFKDAAS LAARIASLWP AGEAYRFVVC TGGEPMLQLD QPLVDALHAA GFTIAIETNG SLPVLESIDW ICVSPKADAP LVVTKGNELK VVVPQDNQRL ADYAKLDFEH FLVQPMDGPS RELNTRLAID WCKRHPRWRL SMQTHKYLNI P
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgtacacg gtcaaggaaa ttttctatac gttgcagggc gagggcgcga acgccggccg tccggccgtg ttctgccggt tcgccggctg caacctctgg acggggcgcg aagcggaccg cgacggcgcg gtgtgccgct tctgcgatac cgacttcgtc ggcaccgacg gcgagaacgg cggcaagttc aaggatgccg cttcgctcgc ggcgaggatc gcgtcgctct ggccggcggg cgaggcgtat cgcttcgtcg tctgcacggg cggcgagccg atgctgcagc tcgatcagcc gctcgtcgac gcgctgcacg cggcgggctt cacgatcgcg atcgagacga acggctcgct gcccgtgctc gaatcgatcg actggatctg cgtgagcccg aaggcggatg cgccgctcgt cgtcacgaag ggcaacgaac tgaaggtggt ggtgccgcag gacaaccagc gccttgccga ttacgcgaag ctcgacttcg agcatttcct cgtgcagccg atggacggcc cgtcgcgcga gctcaacacc cggctcgcga tcgattggtg caagcgtcat ccgcggtggc ggctgtcgat gcagacgcac aagtacctga acattcccta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BupsA.17331.a.A1.PS01030
PURIFICATION DATe: 4/7/2011
CONCENTRATION: 24.98mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTYTVKEIF YTLQGEGANA GRPAVFCRFA GCNLWTGREA DRDGAVCRFC DTDFVGTDGE NGGKFKDAAS LAARIASLWP AGEAYRFVVC TGGEPMLQLD QPLVDALHAA GFTIAIETNG SLPVLESIDW ICVSPKADAP LVVTKGNELK VVVPQDNQRL ADYAKLDFEH FLVQPMDGPS RELNTRLAID WCKRHPRWRL SMQTHKYLNI P
Validated NT Sequence
ttattaggga atgttcaggt acttgtgcgt ctgcatcgac agccgccacc gcggatgacg cttgcaccaa tcgatcgcga gccgggtgtt gagctcgcgc gacgggccgt ccatcggctg cacgaggaaa tgctcgaagt cgagcttcgc gtaatcggca aggcgctggt tgtcctgcgg caccaccacc ttcagttcgt tgcccttcgt gacgacgagc ggcgcatccg ccttcgggct cacgcagatc cagtcgatcg attcgagcac gggcagcgag ccgttcgtct cgatcgcgat cgtgaagccc gccgcgtgca gcgcgtcgac gagcggctga tcgagctgca gcatcggctc gccgcccgtg cagacgacga agcgatacgc ctcgcccgcc ggccagagcg acgcgatcct cgccgcgagc gaagcggcat ccttgaactt gccgccgttc tcgccgtcgg tgccgacgaa gtcggtatcg cagaagcggc acaccgcgcc gtcgcggtcc gcttcgcgcc ccgtccagag gttgcagccg gcgaaccggc agaacacggc cggacggccg gcgttcgcgc cctcgccctg caacgtatag aaaatttcct tgaccgtgta cgtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYTVKEIF YTLQGEGANA GRPAVFCRFA GCNLWTGREA DRDGAVCRFC DTDFVGTDGE NGGKFKDAAS LAARIASLWP AGEAYRFVVC TGGEPMLQLD QPLVDALHAA GFTIAIETNG SLPVLESIDW ICVSPKADAP LVVTKGNELK VVVPQDNQRL ADYAKLDFEH FLVQPMDGPS RELNTRLAID WCKRHPRWRL SMQTHKYLNI P
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGTACACG GTCAAGGAAA TTTTCTATAC GTTGCAGGGC GAGGGCGCGA ACGCCGGCCG TCCGGCCGTG TTCTGCCGGT TCGCCGGCTG CAACCTCTGG ACGGGGCGCG AAGCGGACCG CGACGGCGCG GTGTGCCGCT TCTGCGATAC CGACTTCGTC GGCACCGACG GCGAGAACGG CGGCAAGTTC AAGGATGCCG CTTCGCTCGC GGCGAGGATC GCGTCGCTCT GGCCGGCGGG CGAGGCGTAT CGCTTCGTCG TCTGCACGGG CGGCGAGCCG ATGCTGCAGC TCGATCAGCC GCTCGTCGAC GCGCTGCACG CGGCGGGCTT CACGATCGCG ATCGAGACGA ACGGCTCGCT GCCCGTGCTC GAATCGATCG ACTGGATCTG CGTGAGCCCG AAGGCGGATG CGCCGCTCGT CGTCACGAAG GGCAACGAAC TGAAGGTGGT GGTGCCGCAG GACAACCAGC GCCTTGCCGA TTACGCGAAG CTCGACTTCG AGCATTTCCT CGTGCAGCCG ATGGACGGCC CGTCGCGCGA GCTCAACACC CGGCTCGCGA TCGATTGGTG CAAGCGTCAT CCGCGGTGGC GGCTGTCGAT GCAGACGCAC AAGTACCTGA ACATTCCCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A