SSGCID: Target Page

BupsA.17325.a

Flagellar basal-body rod protein FlgG

CENTER ID:	BupsA.17325.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17325.a.A1.GE31147	Full length( BupsA.17325.a )		1	262

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.468
RefSeq:	YP_331881.1
UniProt:	Q3JX22

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNRSLYIAAT GMNAQQAQMD VISNNLANVS TNGFKGSRAV FEDLLYQTVR QPGANSTQQT ELPSGLQLGT GVQQVATERL YTQGSLQQTG NSKDVAINGQ GFFQVLMPDG TNAYTRDGSF QTNAQGQLVT SSGYQVIPAI TIPQNANSLT IGSDGVVSVT QPGSNNAVQI GALQIATFIN PAGLDAKGEN LFAETTSSGA PNVSQPGLNG AGTLKQGYVE SSNVNVVQEL VNMIQTQRAY EINSKAVTTS DQMLQTVTQM SR

NT Sequence

GTGAACCGTT CCCTCTACAT CGCCGCGACC GGCATGAACG CGCAGCAAGC GCAGATGGAC GTGATCTCGA ACAACCTCGC GAACGTGAGC ACCAACGGCT TCAAGGGCTC GCGCGCGGTG TTCGAGGATC TGCTGTACCA GACCGTGCGC CAGCCCGGCG CGAACTCGAC GCAGCAGACC GAGCTGCCGT CCGGCCTGCA GCTCGGCACG GGCGTCCAGC AGGTCGCCAC CGAGCGCCTG TACACGCAGG GCAGCCTGCA GCAGACCGGC AACTCGAAGG ACGTCGCGAT CAACGGCCAG GGCTTCTTCC AGGTGCTGAT GCCGGACGGC ACGAACGCGT ACACGCGCGA CGGCTCGTTC CAGACCAACG CGCAGGGCCA GCTCGTCACC TCGAGCGGCT ATCAGGTGAT TCCGGCGATC ACGATCCCGC AGAACGCGAA CTCGCTCACG ATCGGCAGCG ACGGCGTCGT GTCCGTCACG CAGCCTGGCT CGAACAACGC GGTGCAGATC GGCGCGCTGC AGATCGCGAC GTTCATCAAT CCGGCCGGCC TCGACGCGAA GGGCGAGAAC TTGTTCGCCG AGACGACCTC GTCGGGCGCG CCGAACGTAT CGCAGCCGGG CCTGAACGGC GCGGGCACGC TCAAGCAGGG CTACGTCGAA TCGTCGAACG TGAACGTCGT GCAGGAGCTC GTCAACATGA TCCAGACGCA GCGCGCGTAC GAAATCAACA GCAAGGCCGT GACGACGTCC GACCAGATGC TGCAGACCGT CACGCAGATG TCGCGC

Details for BupsA.17325.a.A1.GE31147

HARVESTED ON:	12/21/2010
SEQUENCED ON:	12/27/2010
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVNRSLYIA ATGMNAQQAQ MDVISNNLAN VSTNGFKGSR AVFEDLLYQT VRQPGANSTQ QTELPSGLQL GTGVQQVATE RLYTQGSLQQ TGNSKDVAIN GQGFFQVLMP DGTNAYTRDG SFQTNAQGQL VTSSGYQVIP AITIPQNANS LTIGSDGVVS VTQPGSNNAV QIGALQIATF INPAGLDAKG ENLFAETTSS GAPNVSQPGL NGAGTLKQGY VESSNVNVVQ ELVNMIQTQR AYEINSKAVT TSDQMLQTVT QMSR

Validated NT Sequence

ttattagcgc gacatctgcg tgacggtctg cagcatctgg tcggacgtcg tcacggcctt gctgttgatt tcgtacgcgc gctgcgtctg gatcatgttg acgagctcct gcacgacgtt cacgttcgac gattcgacgt agccctgctt gagcgtgccc gcgccgttca ggcccggctg cgatacgttc ggcgcgcccg acgaggtcgt ctcggcgaac aagttctcgc ccttcgcgtc gaggccggcc ggattgatga acgtcgcgat ctgcagcgcg ccgatctgca ccgcgttgtt cgagccaggc tgcgtgacgg acacgacgcc gtcgctgccg atcgtgagcg agttcgcgtt ctgcgggatc gtgatcgccg gaatcacctg atagccgctc gaggtgacga gctggccctg cgcgttggtc tggaacgagc cgtcgcgcgt gtacgcgttc gtgccgtccg gcatcagcac ctggaagaag ccctggccgt tgatcgcgac gtccttcgag ttgccggtct gctgcaggct gccctgcgtg tacaggcgct cggtggcgac ctgctggacg cccgtgccga gctgcaggcc ggacggcagc tcggtctgct gcgtcgagtt cgcgccgggc tggcgcacgg tctggtacag cagatcctcg aacaccgcgc gcgagccctt gaagccgttg gtgctcacgt tcgcgaggtt gttcgagatc acgtccatct gcgcttgctg cgcgttcatg ccggtcgcgg cgatgtagag ggaacggttc accatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaaccgt tccctctaca tcgccgcgac cggcatgaac gcgcagcaag cgcagatgga cgtgatctcg aacaacctcg cgaacgtgag caccaacggc ttcaagggct cgcgcgcggt gttcgaggat ctgctgtacc agaccgtgcg ccagcccggc gcgaactcga cgcagcagac cgagctgccg tccggcctgc agctcggcac gggcgtccag caggtcgcca ccgagcgcct gtacacgcag ggcagcctgc agcagaccgg caactcgaag gacgtcgcga tcaacggcca gggcttcttc caggtgctga tgccggacgg cacgaacgcg tacacgcgcg acggctcgtt ccagaccaac gcgcagggcc agctcgtcac ctcgagcggc tatcaggtga ttccggcgat cacgatcccg cagaacgcga actcgctcac gatcggcagc gacggcgtcg tgtccgtcac gcagcctggc tcgaacaacg cggtgcagat cggcgcgctg cagatcgcga cgttcatcaa tccggccggc ctcgacgcga agggcgagaa cttgttcgcc gagacgacct cgtcgggcgc gccgaacgta tcgcagccgg gcctgaacgg cgcgggcacg ctcaagcagg gctacgtcga atcgtcgaac gtgaacgtcg tgcaggagct cgtcaacatg atccagacgc agcgcgcgta cgaaatcaac agcaaggccg tgacgacgtc cgaccagatg ctgcagaccg tcacgcagat gtcgcgctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa