SSGCID: Target Page

BupsA.17323.a

Flagellar basal-body rod protein FlgB

CENTER ID:	BupsA.17323.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17323.a.A1.GE31146	Full length( BupsA.17323.a )		1	163

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.463
RefSeq:	YP_331876.1
UniProt:	Q3JX27

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLDKLDAEFA FGRQALDVRA YRQELLSSNI ANADTPGYRA RDVDFSSTLA RALKQDGAAS AGNAAQLPLA QPAGVASGMS MATTSGAHLA GNVKLIPTGG PSDDYGRAQY RIPQQPALDG NTVDLDTERV QFADNAVHYQ AGMTVMTQQI KTMIAAITSN SSS

NT Sequence

ATGCTGGACA AACTCGATGC CGAATTCGCG TTCGGCCGCC AGGCGCTCGA CGTGCGCGCA TATCGGCAGG AACTGCTGTC GTCGAACATC GCGAACGCCG ATACCCCCGG CTACCGCGCG CGCGACGTCG ACTTCTCGTC GACGCTCGCC CGCGCGCTCA AGCAGGACGG CGCGGCGAGC GCCGGCAACG CGGCGCAGTT GCCGCTCGCG CAGCCGGCGG GCGTCGCGAG CGGGATGTCG ATGGCGACGA CCTCGGGCGC GCACCTCGCG GGCAACGTGA AGCTGATTCC GACGGGCGGC CCGAGCGACG ACTACGGCCG CGCGCAGTAC CGGATCCCGC AGCAGCCCGC GCTCGACGGC AACACGGTCG ATCTCGACAC CGAGCGCGTG CAGTTCGCCG ACAACGCGGT GCACTACCAG GCCGGGATGA CGGTGATGAC GCAGCAGATC AAGACGATGA TCGCCGCGAT CACGTCGAAT TCGAGTTCG

Details for BupsA.17323.a.A1.GE31146

HARVESTED ON:	12/21/2010
SEQUENCED ON:	12/27/2010
EXPECTED MW:	19kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLDKLDAEF AFGRQALDVR AYRQELLSSN IANADTPGYR ARDVDFSSTL ARALKQDGAA SAGNAAQLPL AQPAGVASGM SMATTSGAHL AGNVKLIPTG GPSDDYGRAQ YRIPQQPALD GNTVDLDTER VQFADNAVHY QAGMTVMTQQ IKTMIAAITS NSSS

Validated NT Sequence

ttattacgaa ctcgaattcg acgtgatcgc ggcgatcatc gtcttgatct gctgcgtcat caccgtcatc ccggcctggt agtgcaccgc gttgtcggcg aactgcacgc gctcggtgtc gagatcgacc gtgttgccgt cgagcgcggg ctgctgcggg atccggtact gcgcgcggcc gtagtcgtcg ctcgggccgc ccgtcggaat cagcttcacg ttgcccgcga ggtgcgcgcc cgaggtcgtc gccatcgaca tcccgctcgc gacgcccgcc ggctgcgcga gcggcaactg cgccgcgttg ccggcgctcg ccgcgccgtc ctgcttgagc gcgcgggcga gcgtcgacga gaagtcgacg tcgcgcgcgc ggtagccggg ggtatcggcg ttcgcgatgt tcgacgacag cagttcctgc cgatatgcgc gcacgtcgag cgcctggcgg ccgaacgcga attcggcatc gagtttgtcc agcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnananngnn gtgntctnnn tnnnnnnnnn nnnnnnnnnn nngtaccgag canannttcn ggctccngnc ntcgcgtcna cgtgggntgc caccgagacc ggaagcccca aggcgtctgc cagcgcgggc cccannggtg cctgccgnca gccgantcgt tggtggtcga ngtggccgct ggnntcatcc accgtgccac cgatcgcgac ccccacccac aggg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctggacaaa ctcgatgccg aattcgcgtt cggccgccag gcgctcgacg tgcgcgcata tcggcaggaa ctgctgtcgt cgaacatcgc gaacgccgat acccccggct accgcgcgcg cgacgtcgac ttctcgtcga cgctcgcccg cgcgctcaag caggacggcg cggcgagcgc cggcaacgcg gcgcagttgc cgctcgcgca gccggcgggc gtcgcgagcg ggatgtcgat ggcgacgacc tcgggcgcgc acctcgcggg caacgtgaag ctgattccga cgggcggccc gagcgacgac tacggccgcg cgcagtaccg gatcccgcag cagcccgcgc tcgacggcaa cacggtcgat ctcgacaccg agcgcgtgca gttcgccgac aacgcggtgc actaccaggc cgggatgacg gtgatgacgc agcagatcaa gacgatgatc gccgcgatca cgtcgaattc gagttcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa