SSGCID: Target Page

BupsA.17311.a

ABC transporter, periplasmic glycine/betaine-binding protein

CENTER ID:	BupsA.17311.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17311.a.A2.GE31136	Mature protein( BupsA.17311.a )		25	316

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17311.a.A2.PW31027	Mature protein( BupsA.17311.a )		25	316

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.6291
RefSeq:	YP_337278.1
UniProt:	Q3JGM5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKSTTTFVFG AALAAACALP SQSFAQDSAA CRNVRFADIG WTDITSTTAL ASLLFDGLGY KPTTTIASVP ISFAGLKNRQ LDVSLGYWWP VQQHQLQPFL DSKSISVVEP PNLSGAKATL AVPSYVYQAG LKSFDDIAKH RAELDGKIYG IEPGSSANAT IQKMIDTNQY GLGGFKLVES SEAGMLVTVE RAIRDKKWVV FLGWEPHPMN IQIGMNYLSG GDAAFGPNYG EARVYTLTSP DYMARCPNAG KLVGNLRFTT QMENQLMQAV MNKVKPAEAA KAYIRKNPQV LDAWLAGVKT YDGKDGLAAV KAYLGL

NT Sequence

ATGAAGTCCA CCACGACATT CGTATTCGGC GCGGCGCTCG CCGCGGCATG CGCGCTGCCG TCGCAATCCT TCGCGCAGGA CTCGGCCGCC TGCCGCAACG TGCGCTTCGC GGACATCGGC TGGACCGACA TCACGTCGAC GACGGCGCTC GCGTCGCTGC TGTTCGACGG TCTAGGCTAC AAGCCGACGA CGACGATCGC GTCCGTGCCG ATTTCGTTCG CAGGACTCAA GAACAGGCAG CTCGACGTAT CGCTCGGCTA CTGGTGGCCG GTGCAGCAGC ATCAGTTGCA GCCGTTCCTC GATTCGAAAT CGATCTCGGT GGTCGAGCCG CCGAACCTGT CGGGCGCGAA GGCGACGCTC GCGGTGCCGA GCTACGTGTA CCAGGCCGGG CTGAAATCGT TCGACGACAT CGCGAAGCAT CGCGCCGAGC TCGACGGCAA GATCTACGGG ATCGAGCCCG GCAGCAGCGC GAACGCGACG ATCCAGAAGA TGATCGACAC GAACCAGTAC GGGCTCGGCG GTTTCAAGCT CGTCGAATCG AGCGAGGCGG GGATGCTCGT CACGGTCGAG CGCGCGATTC GCGACAAGAA GTGGGTCGTG TTCCTCGGCT GGGAGCCGCA TCCGATGAAC ATCCAGATCG GCATGAACTA CCTGTCGGGC GGCGACGCGG CGTTCGGCCC GAACTACGGC GAAGCGCGCG TGTACACGCT GACGTCGCCC GATTACATGG CGCGCTGCCC GAACGCGGGC AAGCTCGTCG GCAATCTGCG CTTCACCACG CAAATGGAAA ACCAGCTGAT GCAGGCGGTG ATGAACAAGG TGAAGCCCGC GGAAGCGGCG AAGGCGTACA TCCGAAAGAA TCCGCAAGTG CTCGATGCGT GGCTTGCCGG CGTGAAGACC TACGACGGCA AGGACGGGCT GGCTGCGGTG AAGGCTTATC TGGGGCTC

Details for BupsA.17311.a.A2.GE31136

HARVESTED ON:	12/21/2010
SEQUENCED ON:	12/27/2010
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMAQDSAA CRNVRFADIG WTDITSTTAL ASLLFDGLGY KPTTTIASVP ISFAGLKNRQ LDVSLGYWWP VQQHQLQPFL DSKSISVVEP PNLSGAKATL AVPSYVYQAG LKSFDDIAKH RAELDGKIYG IEPGSSANAT IQKMIDTNQY GLGGFKLVES SEAGMLVTVE RAIRDKKWVV FLGWEPHPMN IQIGMNYLSG GDAAFGPNYG EARVYTLTSP DYMARCPNAG KLVGNLRFTT QMENQLMQAV MNKVKPAEAA KAYIRKNPQV LDAWLAGVKT YDGKDGLAAV KAYLGL

Validated NT Sequence

ttattagagc cccagataag ccttcaccgc agccagcccg tccttgccgt cgtaggtctt cacgccggca agccacgcat cgagcacttg cggattcttt cggatgtacg ccttcgccgc ttccgcgggc ttcaccttgt tcatcaccgc ctgcatcagc tggttttcca tttgcgtggt gaagcgcaga ttgccgacga gcttgcccgc gttcgggcag cgcgccatgt aatcgggcga cgtcagcgtg tacacgcgcg cttcgccgta gttcgggccg aacgccgcgt cgccgcccga caggtagttc atgccgatct ggatgttcat cggatgcggc tcccagccga ggaacacgac ccacttcttg tcgcgaatcg cgcgctcgac cgtgacgagc atccccgcct cgctcgattc gacgagcttg aaaccgccga gcccgtactg gttcgtgtcg atcatcttct ggatcgtcgc gttcgcgctg ctgccgggct cgatcccgta gatcttgccg tcgagctcgg cgcgatgctt cgcgatgtcg tcgaacgatt tcagcccggc ctggtacacg tagctcggca ccgcgagcgt cgccttcgcg cccgacaggt tcggcggctc gaccaccgag atcgatttcg aatcgaggaa cggctgcaac tgatgctgct gcaccggcca ccagtagccg agcgatacgt cgagctgcct gttcttgagt cctgcgaacg aaatcggcac ggacgcgatc gtcgtcgtcg gcttgtagcc tagaccgtcg aacagcagcg acgcgagcgc cgtcgtcgac gtgatgtcgg tccagccgat gtccgcgaag cgcacgttgc ggcaggcggc cgagtcctgc gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagcc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAQDSAACR NVRFADIGWT DITSTTALAS LLFDGLGYKP TTTIASVPIS FAGLKNRQLD VSLGYWWPVQ QHQLQPFLDS KSISVVEPPN LSGAKATLAV PSYVYQAGLK SFDDIAKHRA ELDGKIYGIE PGSSANATIQ KMIDTNQYGL GGFKLVESSE AGMLVTVERA IRDKKWVVFL GWEPHPMNIQ IGMNYLSGGD AAFGPNYGEA RVYTLTSPDY MARCPNAGKL VGNLRFTTQM ENQLMQAVMN KVKPAEAAKA YIRKNPQVLD AWLAGVKTYD GKDGLAAVKA YLGL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcaggac tcggccgcct gccgcaacgt gcgcttcgcg gacatcggct ggaccgacat cacgtcgacg acggcgctcg cgtcgctgct gttcgacggt ctaggctaca agccgacgac gacgatcgcg tccgtgccga tttcgttcgc aggactcaag aacaggcagc tcgacgtatc gctcggctac tggtggccgg tgcagcagca tcagttgcag ccgttcctcg attcgaaatc gatctcggtg gtcgagccgc cgaacctgtc gggcgcgaag gcgacgctcg cggtgccgag ctacgtgtac caggccgggc tgaaatcgtt cgacgacatc gcgaagcatc gcgccgagct cgacggcaag atctacggga tcgagcccgg cagcagcgcg aacgcgacga tccagaagat gatcgacacg aaccagtacg ggctcggcgg tttcaagctc gtcgaatcga gcgaggcggg gatgctcgtc acggtcgagc gcgcgattcg cgacaagaag tgggtcgtgt tcctcggctg ggagccgcat ccgatgaaca tccagatcgg catgaactac ctgtcgggcg gcgacgcggc gttcggcccg aactacggcg aagcgcgcgt gtacacgctg acgtcgcccg attacatggc gcgctgcccg aacgcgggca agctcgtcgg caatctgcgc ttcaccacgc aaatggaaaa ccagctgatg caggcggtga tgaacaaggt gaagcccgcg gaagcggcga aggcgtacat ccgaaagaat ccgcaagtgc tcgatgcgtg gcttgccggc gtgaagacct acgacggcaa ggacgggctg gctgcggtga aggcttatct ggggctctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BupsA.17311.a.A2.PW31027

PURIFICATION DATe:	2/25/2011
CONCENTRATION:	38.25mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCAGGAC TCGGCCGCCT GCCGCAACGT GCGCTTCGCG GACATCGGCT GGACCGACAT CACGTCGACG ACGGCGCTCG CGTCGCTGCT GTTCGACGGT CTAGGCTACA AGCCGACGAC GACGATCGCG TCCGTGCCGA TTTCGTTCGC AGGACTCAAG AACAGGCAGC TCGACGTATC GCTCGGCTAC TGGTGGCCGG TGCAGCAGCA TCAGTTGCAG CCGTTCCTCG ATTCGAAATC GATCTCGGTG GTCGAGCCGC CGAACCTGTC GGGCGCGAAG GCGACGCTCG CGGTGCCGAG CTACGTGTAC CAGGCCGGGC TGAAATCGTT CGACGACATC GCGAAGCATC GCGCCGAGCT CGACGGCAAG ATCTACGGGA TCGAGCCCGG CAGCAGCGCG AACGCGACGA TCCAGAAGAT GATCGACACG AACCAGTACG GGCTCGGCGG TTTCAAGCTC GTCGAATCGA GCGAGGCGGG GATGCTCGTC ACGGTCGAGC GCGCGATTCG CGACAAGAAG TGGGTCGTGT TCCTCGGCTG GGAGCCGCAT CCGATGAACA TCCAGATCGG CATGAACTAC CTGTCGGGCG GCGACGCGGC GTTCGGCCCG AACTACGGCG AAGCGCGCGT GTACACGCTG ACGTCGCCCG ATTACATGGC GCGCTGCCCG AACGCGGGCA AGCTCGTCGG CAATCTGCGC TTCACCACGC AAATGGAAAA CCAGCTGATG CAGGCGGTGA TGAACAAGGT GAAGCCCGCG GAAGCGGCGA AGGCGTACAT CCGAAAGAAT CCGCAAGTGC TCGATGCGTG GCTTGCCGGC GTGAAGACCT ACGACGGCAA GGACGGGCTG GCTGCGGTGA AGGCTTATCT GGGGCTCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA