BupsA.17307.c

Transposase

CENTER ID: BupsA.17307.c
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17307.c.A1.GE31126 Full length( BupsA.17307.c ) 1 406
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.496
RefSeq: YP_331910.1
UniProt: Q3JWZ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLDRKALQAL GCWTGYRLER VEWPQGDSRT LSLYLKPVSQ IMYCEQCGAR CQQIHETTVR RVRDLPLFEY RVVLHVPRRR VWCERCGAAR LEKLDWLGRY QRVTERFAKA CEKLLQAASV QAVAAFYELG WHTVKSIDKM RLRARVAEPD WSTIRYLAMD EFALHKGHRY ATVVVDPIGR QVLWVGPGRS RETARAFFEQ LPEGVAERIE AVAIDMTTAY ELEIKEQCPQ AEIVFDLYHV VAKYGREVID RVRVDQANQL RHDKPARKVL KSSRWLLLRN RHNLKPEQAV HLKELLAANQ SLLCVYVLRD ELKRLWFYRK PAWAEKAWGQ WFEQAQQSGI AALQKFAQRL QGYWHGIVAR CRHPLNTSVV EGINNTIKVI KRRAYGYRDE QYFFLKIRAA FPGIPR
NT Sequence
TTGCTTGATC GCAAGGCACT TCAGGCACTA GGTTGCTGGA CAGGCTATCG GCTGGAGCGG GTGGAGTGGC CGCAGGGCGA TAGCCGCACG CTGTCGCTCT ACCTGAAGCC GGTCAGTCAG ATCATGTACT GCGAGCAATG CGGTGCGCGT TGCCAGCAGA TTCATGAAAC GACCGTACGG CGGGTACGTG ATCTGCCGTT GTTCGAGTAC CGGGTGGTGC TGCACGTGCC TCGACGCCGA GTCTGGTGCG AACGCTGCGG CGCAGCGCGG CTGGAGAAGC TGGACTGGCT GGGCCGCTAC CAGCGGGTGA CGGAGCGGTT TGCCAAGGCC TGCGAGAAGT TGCTGCAGGC CGCCAGCGTA CAGGCCGTGG CGGCCTTCTA CGAACTGGGC TGGCACACGG TCAAATCGAT CGACAAGATG CGCTTGCGCG CGCGCGTGGC CGAACCGGAC TGGTCGACGA TCCGTTATCT GGCGATGGAC GAGTTCGCGC TCCATAAAGG CCATCGCTAC GCCACGGTGG TGGTTGATCC GATCGGCCGA CAGGTCCTCT GGGTTGGGCC CGGACGGTCA CGCGAGACGG CGCGCGCCTT CTTCGAACAA CTCCCCGAAG GCGTGGCCGA GCGCATCGAA GCGGTCGCAA TCGACATGAC CACGGCCTAT GAGCTGGAGA TCAAGGAACA GTGCCCGCAG GCGGAAATCG TCTTTGACCT GTACCACGTC GTGGCCAAGT ACGGTCGCGA GGTGATCGAT CGGGTACGGG TGGATCAGGC CAACCAACTG CGACATGACA AGCCGGCCCG CAAGGTTCTG AAGTCCAGTC GCTGGTTGCT GCTGCGCAAC CGTCATAACC TGAAGCCAGA ACAGGCCGTG CATCTGAAGG AACTGCTGGC GGCCAATCAG TCGCTGTTAT GCGTCTATGT GCTGCGCGAC GAGCTCAAAC GGCTCTGGTT CTACCGCAAG CCGGCCTGGG CGGAAAAGGC TTGGGGGCAA TGGTTCGAAC AGGCTCAGCA AAGCGGGATC GCCGCCTTGC AAAAGTTCGC CCAGCGCTTG CAGGGTTACT GGCACGGAAT CGTGGCCCGC TGCCGCCATC CGCTCAATAC CAGCGTCGTC GAAGGCATCA ACAACACGAT CAAGGTCATC AAGCGCCGAG CTTACGGGTA CCGCGACGAG CAATACTTCT TCCTCAAGAT CCGCGCCGCG TTCCCCGGGA TTCCGCGA
Details for BupsA.17307.c.A1.GE31126
HARVESTED ON: 12/21/2010
SEQUENCED ON: 12/27/2010
EXPECTED MW: 50kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 62
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLLDRKALQ ALGCWTGYRL ERVEWPQGDS RTLSLYLKPV SQIMYCEQCG ARCQQIHETT VRRVRDLPLF EYRVVLHVPR RXVWCERCGA ARLEKLDWLG RYQRVTERFA KACEKLLQAA SVQAVAAFYE LGWHTVKSID KMRLRARVAE PDWSTIRYLA MDEFALHKGH RYXTVVVDPI GRQVLWVGPG RSRETXRAFF XQLPEGVAXR IXXVAIDMTT AYXLEIXEXC PXXEIVFDLY HXXXXYXAX
Validated NT Sequence
annnnnnnnn nnntacttta agaaggagan ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcttgat cgcaaggcac ttcaggcact aggttgctgg acaggctatc ggctggagcg ggtggagtgg ccgcagggcg atagccgcac gctgtcgctc tacctgaagc cggtcagtca gatcatgtac tgcgagcaat gcggtgcgcg ttgccagcag attcatgaaa cgaccgtacg gcgggtacgt gatctgccgt tgttcgagta ccgggtggtg ctgcacgtgc ctcgacgccn agtctggtgc gaacgctgcg gcgcagcgcg gctggagaag ctggactggc tgggccgcta ccagcgggtg acggagcggt ttgccaaggc ctgcgagaag ttgctgcagg ccgccagcgt acaggccgtg gcggccttct acgaactggg ctggcacacg gtcaaatcga tcgacaagat gcgcttgcgc gcgcgcgtgg ccgaaccgga ctggtcgacg atccgttatc tggcgatgga cgagttcgcg ctccataaag gccatcgcta cnccacggtg gtggttgatc cgatcggccg acaggtcctc tgggttgggc ccggacggtc acgcgagacg gngcgcgcct tcttcnaaca actccccgaa ggcgtggccn agcgcatcnn ancggtcgca atcgacatga ccacggccta tganctggag atcaangaac ngtgcccnna nncggaaatc gtctttgacc tgtaccacnt cnngnncnan tacntcgcnn ngtgancnat cnggntnnng gnnnggatca ngnnnaanct nncnnnntga nnngnnnncc cgnnnngnnn cnnaagnncn nncgnnntgc tncgcannnn nncatnnnnn nnannnnnnn nnnnnnnnng cntctgnann nnnnnngnnn gnnnnnnnnt cnnnnnntnn nnnncnntgn nnnannnnnn nnannnnnnn ntnnnnnnnn nnnnnnnnnn nngnnnnnnn nnntnnnnnn nnnnnnnnna annnnncncn ncnnnnnnan nnnnncnnnn nnnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLLDRKALQ ALGCWTGYRL ERVEWPQGDS RTLSLYLKPV SQIMYCEQCG ARCQQIHETT VRRVRDLPLF EYRVVLHVPR RRVWCERCGA ARLEKLDWLG RYQRVTERFA KACEKLLQAA SVQAVAAFYE LGWHTVKSID KMRLRARVAE PDWSTIRYLA MDEFALHKGH RYATVVVDPI GRQVLWVGPG RSRETARAFF EQLPEGVAER IEAVAIDMTT AYELEIKEQC PQAEIVFDLY HVVAKYGREV IDRVRVDQAN QLRHDKPARK VLKSSRWLLL RNRHNLKPEQ AVHLKELLAA NQSLLCVYVL RDELKRLWFY RKPAWAEKAW GQWFEQAQQS GIAALQKFAQ RLQGYWHGIV ARCRHPLNTS VVEGINNTIK VIKRRAYGYR DEQYFFLKIR AAFPGIPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcttgat cgcaaggcac ttcaggcact aggttgctgg acaggctatc ggctggagcg ggtggagtgg ccgcagggcg atagccgcac gctgtcgctc tacctgaagc cggtcagtca gatcatgtac tgcgagcaat gcggtgcgcg ttgccagcag attcatgaaa cgaccgtacg gcgggtacgt gatctgccgt tgttcgagta ccgggtggtg ctgcacgtgc ctcgacgccg agtctggtgc gaacgctgcg gcgcagcgcg gctggagaag ctggactggc tgggccgcta ccagcgggtg acggagcggt ttgccaaggc ctgcgagaag ttgctgcagg ccgccagcgt acaggccgtg gcggccttct acgaactggg ctggcacacg gtcaaatcga tcgacaagat gcgcttgcgc gcgcgcgtgg ccgaaccgga ctggtcgacg atccgttatc tggcgatgga cgagttcgcg ctccataaag gccatcgcta cgccacggtg gtggttgatc cgatcggccg acaggtcctc tgggttgggc ccggacggtc acgcgagacg gcgcgcgcct tcttcgaaca actccccgaa ggcgtggccg agcgcatcga agcggtcgca atcgacatga ccacggccta tgagctggag atcaaggaac agtgcccgca ggcggaaatc gtctttgacc tgtaccacgt cgtggccaag tacggtcgcg aggtgatcga tcgggtacgg gtggatcagg ccaaccaact gcgacatgac aagccggccc gcaaggttct gaagtccagt cgctggttgc tgctgcgcaa ccgtcataac ctgaagccag aacaggccgt gcatctgaag gaactgctgg cggccaatca gtcgctgtta tgcgtctatg tgctgcgcga cgagctcaaa cggctctggt tctaccgcaa gccggcctgg gcggaaaagg cttgggggca atggttcgaa caggctcagc aaagcgggat cgccgccttg caaaagttcg cccagcgctt gcagggttac tggcacggaa tcgtggcccg ctgccgccat ccgctcaata ccagcgtcgt cgaaggcatc aacaacacga tcaaggtcat caagcgccga gcttacgggt accgcgacga gcaatacttc ttcctcaaga tccgcgccgc gttccccggg attccgcgat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa