SSGCID: Target Page

BupsA.17307.b

ISBma1, transposase

CENTER ID:	BupsA.17307.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17307.b.A1.GE31125	Full length( BupsA.17307.b )		1	191

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.5568
RefSeq:	YP_336617.1
UniProt:	Q3JII6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTAYELEIK EQCPQAEIVF DLYHVVAKYG REVIDRVRVD QANQLRHDKP ARKVLKSSRW LLLRNRHNLK PEQAVHLKEL LAANQSLLCV YVLRDELKRL WFYRKPACAE KAWGQWFEQA QQSGIAALQK FAQRLQGYWH GIVARCRHPL NTSVVEGINN TIKVIKRRAY GYRDEQYFFL KIRAAFPGIP R

NT Sequence

ATGACCACGG CCTATGAGCT GGAGATCAAG GAACAGTGCC CACAGGCGGA AATCGTCTTT GACCTGTACC ACGTCGTGGC CAAGTACGGT CGCGAGGTGA TCGATCGGGT ACGGGTGGAT CAGGCCAACC AACTGCGACA TGACAAGCCG GCCCGCAAGG TTCTGAAGTC CAGTCGCTGG TTGCTGCTGC GCAACCGTCA TAACCTGAAG CCAGAACAGG CCGTGCATCT GAAGGAACTG CTGGCGGCCA ATCAGTCGCT GTTATGCGTC TATGTGCTGC GCGACGAGCT CAAACGGCTC TGGTTCTACC GCAAGCCGGC CTGCGCGGAA AAGGCTTGGG GGCAATGGTT CGAACAGGCT CAGCAAAGCG GGATCGCCGC CTTGCAAAAG TTCGCCCAGC GCTTGCAGGG TTACTGGCAC GGAATCGTGG CCCGCTGCCG CCATCCGCTC AATACCAGCG TCGTCGAAGG CATCAACAAC ACGATCAAGG TCATCAAGCG CCGAGCTTAC GGGTACCGCG ACGAGCAATA CTTCTTCCTC AAGATCCGCG CCGCGTTCCC CGGGATTCCG CGA

Details for BupsA.17307.b.A1.GE31125

HARVESTED ON:	12/21/2010
SEQUENCED ON:	12/27/2010
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTTAYELEI KEQCPQAEIV FDLYHVVAKY GREVIDRVRV DQANQLRHDK PARKVLKSSR WLLLRNRHNL KPEQAVHLKE LLAANQSLLC VYVLRDELKR LWFYRKPAWA EKAWGQWFEQ AQQSGIAALQ KFAQRLQGYW HGIVARCRHP LNTSVVEGIN NTIKVIKRRA YGYRDEQYFF LKIRAAFPGI PR

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca cggcctatga gctggagatc aaggaacagt gcccgcaggc ggaaatcgtc tttgacctgt accacgtcgt ggccaagtac ggtcgcgagg tgatcgatcg ggtacgggtg gatcaggcca accaactgcg acatgacaag ccggcccgca aggttctgaa gtccagtcgc tggttgctgc tgcgcaaccg tcataacctg aagccagaac aggccgtgca tctgaaggaa ctgctggcgg ccaatcagtc gctgttatgc gtctatgtgc tgcgcgacga gctcaaacgg ctctggttct accgcaagcc ggcctgggcg gaaaaggctt gggggcaatg gttcgaacag gctcagcaaa gcgggatcgc cgccttgcaa aagttcgccc agcgcttgca gggttactgg cacggaatcg tggcccgctg ccgccatccg ctcaatacca gcgtcgtcga aggcatcaac aacacgatca aggtcatcaa gcgccgagct tacgggtacc gcgacgagca atacttcttc ctcaagatcc gcgccgcgtt ccccgggatt ccgcgataat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg cnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTTAYELEI KEQCPQAEIV FDLYHVVAKY GREVIDRVRV DQANQLRHDK PARKVLKSSR WLLLRNRHNL KPEQAVHLKE LLAANQSLLC VYVLRDELKR LWFYRKPACA EKAWGQWFEQ AQQSGIAALQ KFAQRLQGYW HGIVARCRHP LNTSVVEGIN NTIKVIKRRA YGYRDEQYFF LKIRAAFPGI PR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccacggcc tatgagctgg agatcaagga acagtgccca caggcggaaa tcgtctttga cctgtaccac gtcgtggcca agtacggtcg cgaggtgatc gatcgggtac gggtggatca ggccaaccaa ctgcgacatg acaagccggc ccgcaaggtt ctgaagtcca gtcgctggtt gctgctgcgc aaccgtcata acctgaagcc agaacaggcc gtgcatctga aggaactgct ggcggccaat cagtcgctgt tatgcgtcta tgtgctgcgc gacgagctca aacggctctg gttctaccgc aagccggcct gcgcggaaaa ggcttggggg caatggttcg aacaggctca gcaaagcggg atcgccgcct tgcaaaagtt cgcccagcgc ttgcagggtt actggcacgg aatcgtggcc cgctgccgcc atccgctcaa taccagcgtc gtcgaaggca tcaacaacac gatcaaggtc atcaagcgcc gagcttacgg gtaccgcgac gagcaatact tcttcctcaa gatccgcgcc gcgttccccg ggattccgcg ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa