SSGCID: Target Page

BupsA.17307.a

Transposase

CENTER ID:	BupsA.17307.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17307.a.A1.GE31124	Full length( BupsA.17307.a )		1	174

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.5569
RefSeq:	YP_336618.1
UniProt:	Q3JII5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYCEQCGARC QQIHETTVRR VRDLPLFEYR VVLHVPRRRV WCERCGAARL EKLDWLGRYQ RVTERFAKAC EKLLQAASVQ AVAAFYELGW HTVKSIDKMR LRARVAEPDW STIRYLAMDE FALHKGHRYA TVVVDPIGRQ VLWVGPGRSR ETARASSNNS PKAWPSASKR SQST

NT Sequence

ATGTACTGCG AGCAATGCGG TGCGCGTTGC CAGCAGATTC ATGAAACGAC CGTACGGCGG GTACGTGATC TGCCGTTGTT CGAGTACCGG GTGGTGCTGC ACGTGCCTCG ACGCCGAGTC TGGTGCGAAC GCTGCGGCGC AGCGCGGCTG GAGAAGCTGG ACTGGCTGGG CCGCTACCAG CGGGTGACGG AGCGGTTTGC CAAGGCCTGC GAGAAGTTGC TGCAGGCCGC CAGCGTACAG GCCGTGGCGG CCTTCTACGA ACTGGGCTGG CACACGGTCA AATCGATCGA CAAGATGCGC TTGCGCGCGC GCGTGGCCGA ACCGGACTGG TCGACGATCC GTTATCTGGC GATGGACGAG TTCGCGCTCC ATAAAGGCCA TCGCTACGCC ACGGTGGTGG TTGATCCGAT CGGCCGACAG GTCCTCTGGG TTGGGCCCGG ACGGTCACGC GAGACGGCGC GCGCTTCTTC GAACAACTCC CCGAAGGCGT GGCCGAGCGC ATCGAAGCGG TCGCAATCGA CA

Details for BupsA.17307.a.A1.GE31124

HARVESTED ON:	12/21/2010
SEQUENCED ON:	12/27/2010
EXPECTED MW:	22kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	91
PERCENT COVERAGE:	109

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMYCEQCGAR CQQIHETTVR RVRDLPLFEY RVVLHVPRRR VWCERCGAAR LEKLDWLGRY QRVTERFAKA CEKLLQAASV QAVAAFYELG WHTVKSIDKM RLRARVAEPD WSTIRYLAMD EFALHKGHRY ATVVVDPIGR QVLWVGPGRS RETARAFFEQ LPEGVAERIE AVAIDIINST NKFCSQASRG SGC

Validated NT Sequence

ttnnntttaa gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg tactgcgagc aatgcggtgc gcgttgccag cagattcatg aaacgaccgt acggcgggta cgtgatctgc cgttgttcga gtaccgggtg gtgctgcacg tgcctcgacg ccgagtctgg tgcgaacgct gcggcgcagc gcggctggag aagctggact ggctgggccg ctaccagcgg gtgacggagc ggtttgccaa ggcctgcgag aagttgctgc aggccgccag cgtacaggcc gtggcggcct tctacgaact gggctggcac acggtcaaat cgatcgacaa gatgcgcttg cgcgcgcgcg tggccgaacc ggactggtcg acgatccgtt atctggcgat ggacgagttc gcgctccata aaggccatcg ctacgccacg gtggtggttg atccgatcgg ccgacaggtc ctctgggttg ggcccggacg gtcacgcgag acggcgcgcg ccttcttcga acaactcccc gaaggcgtgg ccgagcgcat cgaagcggtc gcaatcgaca taataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc nnnnnnnnnn aaaaaaaaan nnaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtactgcgag caatgcggtg cgcgttgcca gcagattcat gaaacgaccg tacggcgggt acgtgatctg ccgttgttcg agtaccgggt ggtgctgcac gtgcctcgac gccgagtctg gtgcgaacgc tgcggcgcag cgcggctgga gaagctggac tggctgggcc gctaccagcg ggtgacggag cggtttgcca aggcctgcga gaagttgctg caggccgcca gcgtacaggc cgtggcggcc ttctacgaac tgggctggca cacggtcaaa tcgatcgaca agatgcgctt gcgcgcgcgc gtggccgaac cggactggtc gacgatccgt tatctggcga tggacgagtt cgcgctccat aaaggccatc gctacgccac ggtggtggtt gatccgatcg gccgacaggt cctctgggtt gggcccggac ggtcacgcga gacggcgcgc gcttcttcga acaactcccc gaaggcgtgg ccgagcgcat cgaagcggtc gcaatcgaca taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa