BupsA.17300.a

Type I secretion outer membrane protein, TolC family

CENTER ID: BupsA.17300.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17300.a.B2.GE36556 Mature protein( BupsA.17300.a ) 23 479
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3774
RefSeq: YP_334993.1
UniProt: Q3JN60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKPARTLAMA GALALFASQA THAQWLDLYG TRKNVSASPA APMLKNATCQ PELADRPIEL EDAILQAICA NPQARRAWAD ARAQAAQLGV KEAAYLPTLN ATAGIQRNWQ TTTYEQDLGP ITLTADQKQI QTSRYGALNL SWVLFDFGKR SAALRQAREL LAAANATQDD TLQTILFNAA QAYYNLRDTQ AALDAARTTE RVAQDSLTAA KAKHDAGAGT LSDQLQAQTS YRRAVLDRVS AEGDVRNATG VLAVAMGLDA NTPLRIATAE PPVDRNAFAE GIAQLIDEAK RQHPKLVAAR AKLDAARANV DAVRAQGRPT ISLTGSLSRN SPSYQQQPGI QTTASHGSMI GVQVTIPLFE GFASGYRVAA AQAQADAQEA DVQNTELNVS LDVWKSYQSL QTDTANLDNS KDLLGDAQRS LDIARGRYKA GVGTFTELLN AQTALADAQK QRVQALSKWR TARLKLAASL GSIGLWSAH
NT Sequence
ATGAAGCCCG CACGCACCCT CGCCATGGCC GGCGCGCTCG CGCTCTTCGC ATCGCAAGCG ACGCACGCGC AATGGCTCGA TCTCTACGGA ACCCGAAAGA ATGTGTCGGC TTCTCCCGCC GCGCCGATGC TGAAGAACGC GACATGCCAG CCCGAGCTCG CGGACCGTCC GATCGAGCTC GAAGACGCGA TCCTGCAGGC GATCTGCGCG AACCCGCAGG CACGGCGCGC GTGGGCCGAT GCGCGCGCGC AGGCCGCCCA GCTCGGCGTG AAGGAGGCGG CGTATCTGCC GACGCTCAAT GCAACAGCGG GCATTCAGCG CAACTGGCAA ACGACGACGT ATGAGCAGGA CCTCGGCCCG ATCACGCTCA CCGCCGACCA GAAGCAGATC CAGACGAGCC GCTACGGCGC GTTGAACCTG AGCTGGGTGC TGTTCGATTT CGGCAAGCGC AGCGCGGCGC TCAGGCAGGC GCGCGAGCTC CTCGCCGCCG CCAACGCGAC GCAGGACGAC ACGCTCCAGA CCATCCTGTT CAATGCCGCC CAGGCGTATT ACAACCTGCG CGACACGCAG GCGGCGCTCG ACGCGGCGCG CACGACCGAA CGCGTCGCGC AGGATAGCCT CACCGCGGCG AAGGCGAAGC ACGACGCCGG CGCCGGCACG CTGAGCGATC AACTGCAGGC GCAAACGAGC TATCGCCGCG CGGTGCTCGA TCGCGTGAGC GCCGAAGGCG ACGTGCGGAA CGCGACGGGC GTGCTCGCCG TCGCGATGGG GCTCGACGCG AACACGCCGC TGCGGATCGC GACCGCCGAG CCGCCGGTCG ATCGCAACGC GTTCGCCGAA GGCATCGCGC AATTGATCGA CGAGGCGAAG CGGCAGCATC CGAAGCTCGT CGCCGCGCGC GCGAAACTCG ACGCGGCGCG CGCGAACGTC GATGCGGTGC GTGCGCAGGG CCGCCCGACC ATCTCGCTGA CCGGCAGCCT CAGCCGCAAC AGCCCGTCGT ATCAGCAGCA GCCGGGCATC CAGACCACCG CGAGCCACGG CAGCATGATC GGCGTGCAGG TGACGATCCC GCTCTTCGAA GGCTTCGCGT CCGGCTACCG CGTCGCGGCC GCGCAGGCAC AGGCGGATGC GCAGGAGGCC GACGTGCAGA ACACCGAGCT GAACGTCTCG CTCGACGTGT GGAAGAGCTA TCAGAGCCTG CAGACCGACA CCGCGAATCT CGACAATTCG AAGGACCTGC TCGGCGATGC GCAACGCTCG CTCGACATCG CACGGGGCCG GTACAAGGCG GGCGTCGGCA CGTTCACGGA GTTGCTCAAT GCGCAGACCG CGCTCGCGGA CGCGCAGAAG CAGCGCGTGC AGGCGCTGTC GAAATGGCGC ACCGCGCGGC TCAAGCTCGC GGCGAGCCTC GGCAGCATCG GCCTGTGGTC CGCCCAT
Details for BupsA.17300.a.B2.GE36556
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 50kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Validated AA Sequence
MLKNATCQPE LADRPIELED AILQAICANP QARRAWADAR AQAAQLGVKE AAYLPTLNAT AGIQRNWQTT TYEQDLGPIT LTADQKQIQT SRYGALNLSW VLFDFGKRSA ALRQARELLA AANATQDDTL QTILFNAAQA YYNLRDTQAA LDAARTTERV AQDSLTAAKA KHDAGAGTLS DQLQAQTSYR RAVLDRVSAE GDVRNATGVL AVAMGLDANT PLRIATAEPP VDRNAFAEGI AQLIDEAKRQ HPKLVAARAK LDAARANVDA VRAQGRPTIS LTGSLSRNSP SYQQQPGIQT TASHGSMIGV QVTIPLFEGF ASGYRVAAAQ AQADAQEADV QNTELNVSLD VWKSYQSLQT DTANLDNSKD LLGDAQRSLD IARGRYKAGV GTFTELLNAQ TALADAQKQR VQALSKWRTA RLKLAASL
Validated NT Sequence
gccgaggctc gccgcgagct tgagccgcgc ggtgcgccat ttcgacagcg cctgcacgcg ctgcttctgc gcgtccgcga gcgcggtctg cgcattgagc aactccgtga acgtgccgac gcccgccttg taccggcccc gtgcgatgtc gagcgagcgt tgcgcatcgc cgagcaggtc cttcgaattg tcgagattcg cggtgtcggt ctgcaggctc tgatagctct tccacacgtc gagcgagacg ttcagctcgg tgttctgcac gtcggcctcc tgcgcatccg cctgtgcctg cgcggccgcg acgcggtagc cggacgcgaa gccttcgaag agcgggatcg tcacctgcac gccgatcatg ctgccgtggc tcgcggtggt ctggatgccc ggctgctgct gatacgacgg gctgttgcgg ctgaggctgc cggtcagcga gatggtcggg cggccctgcg cacgcaccgc atcgacgttc gcgcgcgccg cgtcgagttt cgcgcgcgcg gcgacgagct tcggatgctg ccgcttcgcc tcgtcgatca attgcgcgat gccttcggcg aacgcgttgc gatcgaccgg cggctcggcg gtcgcgatcc gcagcggcgt gttcgcgtcg agccccatcg cgacggcgag cacgcccgtc gcgttccgca cgtcgccttc ggcgctcacg cgatcgagca ccgcgcggcg atagctcgtt tgcgcctgca gttgatcgct cagcgtgccg gcgccggcgt cgtgcttcgc cttcgccgcg gtgaggctat cctgcgcgac gcgttcggtc gtgcgcgccg cgtcgagcgc cgcctgcgtg tcgcgcaggt tgtaatacgc ctgggcggca ttgaacagga tggtctggag cgtgtcgtcc tgcgtcgcgt tggcggcggc gaggagctcg cgcgcctgcc tgagcgccgc gctgcgcttg ccgaaatcga acagcaccca gctcaggttc aacgcgccgt agcggctcgt ctggatctgc ttctggtcgg cggtgagcgt gatcgggccg aggtcctgct catacgtcgt cgtttgccag ttgcgctgaa tgcccgctgt tgcattgagc gtcggcagat acgccgcctc cttcacgccg agctgggcgg cctgcgcgcg cgcatcggcc cacgcgcgcc gtgcctgcgg gttcgcgcag atcgcctgca ggatcgcgtc ttcgagctcg atcggacggt ccgcgagctc gggctggcat gtcgcgttct tcagcatcgg cgcggcggga gaagccgaca cattctttcg ggttccgtag agatcgagcc att
Expected Protein Sequence
MAHHHHHHMA QWLDLYGTRK NVSASPAAPM LKNATCQPEL ADRPIELEDA ILQAICANPQ ARRAWADARA QAAQLGVKEA AYLPTLNATA GIQRNWQTTT YEQDLGPITL TADQKQIQTS RYGALNLSWV LFDFGKRSAA LRQARELLAA ANATQDDTLQ TILFNAAQAY YNLRDTQAAL DAARTTERVA QDSLTAAKAK HDAGAGTLSD QLQAQTSYRR AVLDRVSAEG DVRNATGVLA VAMGLDANTP LRIATAEPPV DRNAFAEGIA QLIDEAKRQH PKLVAARAKL DAARANVDAV RAQGRPTISL TGSLSRNSPS YQQQPGIQTT ASHGSMIGVQ VTIPLFEGFA SGYRVAAAQA QADAQEADVQ NTELNVSLDV WKSYQSLQTD TANLDNSKDL LGDAQRSLDI ARGRYKAGVG TFTELLNAQT ALADAQKQRV QALSKWRTAR LKLAASLGSI GLWSAH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc aatggctcga tctctacgga acccgaaaga atgtgtcggc ttctcccgcc gcgccgatgc tgaagaacgc gacatgccag cccgagctcg cggaccgtcc gatcgagctc gaagacgcga tcctgcaggc gatctgcgcg aacccgcagg cacggcgcgc gtgggccgat gcgcgcgcgc aggccgccca gctcggcgtg aaggaggcgg cgtatctgcc gacgctcaat gcaacagcgg gcattcagcg caactggcaa acgacgacgt atgagcagga cctcggcccg atcacgctca ccgccgacca gaagcagatc cagacgagcc gctacggcgc gttgaacctg agctgggtgc tgttcgattt cggcaagcgc agcgcggcgc tcaggcaggc gcgcgagctc ctcgccgccg ccaacgcgac gcaggacgac acgctccaga ccatcctgtt caatgccgcc caggcgtatt acaacctgcg cgacacgcag gcggcgctcg acgcggcgcg cacgaccgaa cgcgtcgcgc aggatagcct caccgcggcg aaggcgaagc acgacgccgg cgccggcacg ctgagcgatc aactgcaggc gcaaacgagc tatcgccgcg cggtgctcga tcgcgtgagc gccgaaggcg acgtgcggaa cgcgacgggc gtgctcgccg tcgcgatggg gctcgacgcg aacacgccgc tgcggatcgc gaccgccgag ccgccggtcg atcgcaacgc gttcgccgaa ggcatcgcgc aattgatcga cgaggcgaag cggcagcatc cgaagctcgt cgccgcgcgc gcgaaactcg acgcggcgcg cgcgaacgtc gatgcggtgc gtgcgcaggg ccgcccgacc atctcgctga ccggcagcct cagccgcaac agcccgtcgt atcagcagca gccgggcatc cagaccaccg cgagccacgg cagcatgatc ggcgtgcagg tgacgatccc gctcttcgaa ggcttcgcgt ccggctaccg cgtcgcggcc gcgcaggcac aggcggatgc gcaggaggcc gacgtgcaga acaccgagct gaacgtctcg ctcgacgtgt ggaagagcta tcagagcctg cagaccgaca ccgcgaatct cgacaattcg aaggacctgc tcggcgatgc gcaacgctcg ctcgacatcg cacggggccg gtacaaggcg ggcgtcggca cgttcacgga gttgctcaat gcgcagaccg cgctcgcgga cgcgcagaag cagcgcgtgc aggcgctgtc gaaatggcgc accgcgcggc tcaagctcgc ggcgagcctc ggcagcatcg gcctgtggtc cgcccattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at