BupsA.17298.b

Oxygen-independent coproporphyrinogen III oxidase

CENTER ID: BupsA.17298.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17298.b.A1.GE32583 Full length( BupsA.17298.b ) 1 481
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2949
RefSeq: YP_334202.1
UniProt: Q3JQF1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTQSNDGAD TTAPAPQHDV SAFADVQISE ALIRRFDRQG PRYTSYPTAD RFSDAFDERA YREHLSRRAS AERNPPLSVY LHLPFCESLC YFCACNKIIT QDHTRTSAYV DYLIREMELV APDLGRDRRT TQLHLGGGSP TFFAIDELAR LMRALREHFD FAPHAELGVE IDPRTVNERT LQSLAALGFN RTSFGVQDFD PSVQEAVHRI QPLPMVERAL EASRAAGFES VNIDLIYGLP RQTPASFSRT LDEVIRLSPE RIAVYNYAHL PSRFKAQRLI VEAQLPPAED RLRIFIESTR RLLDAGYVYI GLDHFAKPND ELGNALRERS LHRNFQGYTT QAECDLVGFG VSAIGKVGAS YSQSTRSLKT YYRQLDAGRL PIERGFALTA DDLLRREVIM TVMCSTPVDF AEISHRHGID FARYFAPELA QLEPYRDAGL LTIDAQRIAV TPKGRMFVRA IGMVFDAYLG RSAAASYSKL I
NT Sequence
ATGACAACGC AATCGAACGA CGGCGCGGAT ACGACCGCGC CCGCTCCCCA ACACGACGTG TCCGCGTTCG CGGACGTTCA GATCTCCGAA GCGCTGATTC GGCGCTTCGA CCGGCAAGGG CCGCGCTATA CGTCCTATCC GACGGCCGAC CGGTTCTCCG ACGCATTCGA CGAGCGCGCG TACCGCGAAC ATCTGTCGCG CCGCGCGTCA GCCGAGCGTA ATCCGCCGCT GTCGGTCTAT CTGCATCTGC CGTTTTGCGA GTCGCTCTGC TACTTCTGCG CGTGCAACAA GATCATCACG CAGGATCACA CCCGCACGAG CGCGTACGTC GACTATCTGA TCCGCGAAAT GGAGCTCGTC GCGCCGGATC TCGGCCGCGA TCGGCGGACG ACGCAACTGC ATCTGGGCGG CGGCTCGCCG ACGTTCTTCG CGATCGACGA GCTCGCGCGC CTGATGCGCG CGCTGCGCGA GCACTTCGAC TTCGCGCCGC ACGCGGAGCT CGGCGTCGAG ATCGATCCGC GCACGGTCAA CGAGCGCACC CTGCAGTCGC TCGCGGCGCT CGGCTTCAAC CGGACGAGCT TCGGCGTGCA GGACTTCGAT CCGTCGGTGC AGGAGGCGGT GCATCGGATC CAGCCGCTGC CGATGGTCGA GCGCGCGCTC GAGGCGAGCC GCGCGGCCGG TTTCGAATCG GTCAACATCG ATCTGATCTA CGGGTTGCCG CGGCAGACGC CCGCGAGCTT TTCGCGCACG CTCGACGAGG TGATCCGGCT GTCGCCCGAG CGCATCGCGG TCTACAACTA CGCGCATTTG CCGAGCCGCT TCAAGGCGCA GCGCCTGATC GTCGAAGCGC AGCTGCCGCC CGCGGAAGAC CGGCTGCGGA TCTTCATCGA ATCGACGCGG CGGCTGCTCG ACGCGGGCTA CGTGTACATC GGGCTCGATC ACTTCGCGAA GCCGAACGAC GAGCTCGGCA ACGCGCTGCG CGAGCGCAGC CTGCACCGCA ATTTCCAGGG CTATACGACG CAGGCCGAAT GCGATCTCGT CGGCTTCGGC GTATCGGCGA TCGGCAAGGT CGGCGCCTCG TACAGCCAGT CGACGCGCTC GCTGAAGACC TATTACCGCC AGCTCGACGC GGGGCGCCTG CCGATCGAGC GGGGCTTCGC GCTGACAGCC GACGATTTGC TGCGCCGCGA AGTCATCATG ACGGTGATGT GCAGCACACC CGTCGATTTC GCGGAGATCA GCCACAGGCA CGGCATCGAT TTCGCCCGGT ATTTCGCGCC CGAGCTCGCG CAGCTCGAGC CGTATCGCGA CGCGGGGCTG CTCACGATCG ATGCGCAGCG CATCGCCGTC ACGCCGAAGG GGCGCATGTT CGTGCGCGCG ATCGGCATGG TGTTCGACGC GTATCTCGGC CGCAGCGCCG CGGCGTCTTA TTCGAAATTG ATC
Details for BupsA.17298.b.A1.GE32583
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MTTQSNDGAD TTAPAPQHDV SAFADVQISE ALIRRFDRQG PRYTSYPTAD RFSDAFDERA YREHLSRRAS AERNPPLSVY LHLPFCESLC YFCACNKIIT QDHTRTSAYV DYLIREMELV APDLGRDRRT TQLHLGGGSP TFFAIDELAR LMRALREHFD FAPHAELGVE IDPRTVNERT LQSLAALGFN RTSFGVQDFD PSVQEAVHRI QPLPMVERAL EASRAAGFES VNIDLIYGLP RQTPASFSRT LDEVIRLSPE RIAVYNYAHL PSRFKAQRLI VEAQLPPAED RLRIFIESTR RLLDAGYVYI GLDHFAKPND ELGNALRERS LHRNFQGYTT QAECDLVGFG VSAIGKVGAS YSQSTRSLKT YYRQLDAGRL PIERGFALTA DDLLRREVIM TVMCSTPVDF AEISHRHGID FARYFAPELA QLEPYRDAGL LTIDAQRIAV TPKGRMFVRA IGMVFDAYLG RSAAASYSKL I
Validated NT Sequence
ttattagatc aatttcgaat aagacgccgc ggcgctgcgg ccgagatacg cgtcgaacac catgccgatc gcgcgcacga acatgcgccc cttcggcgtg acggcgatgc gctgcgcatc gatcgtgagc agccccgcgt cgcgatacgg ctcgagctgc gcgagctcgg gcgcgaaata ccgggcgaaa tcgatgccgt gcctgtggct gatctccgcg aaatcgacgg gtgtgctgca catcaccgtc atgatgactt cgcggcgcag caaatcgtcg gctgtcagcg cgaagccccg ctcgatcggc aggcgccccg cgtcgagctg gcggtaatag gtcttcagcg agcgcgtcga ctggctgtac gaggcgccga ccttgccgat cgccgatacg ccgaagccga cgagatcgca ttcggcctgc gtcgtatagc cctggaaatt gcggtgcagg ctgcgctcgc gcagcgcgtt gccgagctcg tcgttcggct tcgcgaagtg atcgagcccg atgtacacgt agcccgcgtc gagcagccgc cgcgtcgatt cgatgaagat ccgcagccgg tcttccgcgg gcggcagctg cgcttcgacg atcaggcgct gcgccttgaa gcggctcggc aaatgcgcgt agttgtagac cgcgatgcgc tcgggcgaca gccggatcac ctcgtcgagc gtgcgcgaaa agctcgcggg cgtctgccgc ggcaacccgt agatcagatc gatgttgacc gattcgaaac cggccgcgcg gctcgcctcg agcgcgcgct cgaccatcgg cagcggctgg atccgatgca ccgcctcctg caccgacgga tcgaagtcct gcacgccgaa gctcgtccgg ttgaagccga gcgccgcgag cgactgcagg gtgcgctcgt tgaccgtgcg cggatcgatc tcgacgccga gctccgcgtg cggcgcgaag tcgaagtgct cgcgcagcgc gcgcatcagg cgcgcgagct cgtcgatcgc gaagaacgtc ggcgagccgc cgcccagatg cagttgcgtc gtccgccgat cgcggccgag atccggcgcg acgagctcca tttcgcggat cagatagtcg acgtacgcgc tcgtgcgggt gtgatcctgc gtgatgatct tgttgcacgc gcagaagtag cagagcgact cgcaaaacgg cagatgcaga tagaccgaca gcggcggatt acgctcggct gacgcgcggc gcgacagatg ttcgcggtac gcgcgctcgt cgaatgcgtc ggagaaccgg tcggccgtcg gataggacgt atagcgcggc ccttgccggt cgaagcgccg aatcagcgct tcggagatct gaacgtccgc gaacgcggac acgtcgtgtt ggggagcggg cgcggtcgta tccgcgccgt cgttcgattg cgttgtcatc gaaccaggac cctgggtctg agcttccagg gtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTQSNDGA DTTAPAPQHD VSAFADVQIS EALIRRFDRQ GPRYTSYPTA DRFSDAFDER AYREHLSRRA SAERNPPLSV YLHLPFCESL CYFCACNKII TQDHTRTSAY VDYLIREMEL VAPDLGRDRR TTQLHLGGGS PTFFAIDELA RLMRALREHF DFAPHAELGV EIDPRTVNER TLQSLAALGF NRTSFGVQDF DPSVQEAVHR IQPLPMVERA LEASRAAGFE SVNIDLIYGL PRQTPASFSR TLDEVIRLSP ERIAVYNYAH LPSRFKAQRL IVEAQLPPAE DRLRIFIEST RRLLDAGYVY IGLDHFAKPN DELGNALRER SLHRNFQGYT TQAECDLVGF GVSAIGKVGA SYSQSTRSLK TYYRQLDAGR LPIERGFALT ADDLLRREVI MTVMCSTPVD FAEISHRHGI DFARYFAPEL AQLEPYRDAG LLTIDAQRIA VTPKGRMFVR AIGMVFDAYL GRSAAASYSK LI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaacgcaa tcgaacgacg gcgcggatac gaccgcgccc gctccccaac acgacgtgtc cgcgttcgcg gacgttcaga tctccgaagc gctgattcgg cgcttcgacc ggcaagggcc gcgctatacg tcctatccga cggccgaccg gttctccgac gcattcgacg agcgcgcgta ccgcgaacat ctgtcgcgcc gcgcgtcagc cgagcgtaat ccgccgctgt cggtctatct gcatctgccg ttttgcgagt cgctctgcta cttctgcgcg tgcaacaaga tcatcacgca ggatcacacc cgcacgagcg cgtacgtcga ctatctgatc cgcgaaatgg agctcgtcgc gccggatctc ggccgcgatc ggcggacgac gcaactgcat ctgggcggcg gctcgccgac gttcttcgcg atcgacgagc tcgcgcgcct gatgcgcgcg ctgcgcgagc acttcgactt cgcgccgcac gcggagctcg gcgtcgagat cgatccgcgc acggtcaacg agcgcaccct gcagtcgctc gcggcgctcg gcttcaaccg gacgagcttc ggcgtgcagg acttcgatcc gtcggtgcag gaggcggtgc atcggatcca gccgctgccg atggtcgagc gcgcgctcga ggcgagccgc gcggccggtt tcgaatcggt caacatcgat ctgatctacg ggttgccgcg gcagacgccc gcgagctttt cgcgcacgct cgacgaggtg atccggctgt cgcccgagcg catcgcggtc tacaactacg cgcatttgcc gagccgcttc aaggcgcagc gcctgatcgt cgaagcgcag ctgccgcccg cggaagaccg gctgcggatc ttcatcgaat cgacgcggcg gctgctcgac gcgggctacg tgtacatcgg gctcgatcac ttcgcgaagc cgaacgacga gctcggcaac gcgctgcgcg agcgcagcct gcaccgcaat ttccagggct atacgacgca ggccgaatgc gatctcgtcg gcttcggcgt atcggcgatc ggcaaggtcg gcgcctcgta cagccagtcg acgcgctcgc tgaagaccta ttaccgccag ctcgacgcgg ggcgcctgcc gatcgagcgg ggcttcgcgc tgacagccga cgatttgctg cgccgcgaag tcatcatgac ggtgatgtgc agcacacccg tcgatttcgc ggagatcagc cacaggcacg gcatcgattt cgcccggtat ttcgcgcccg agctcgcgca gctcgagccg tatcgcgacg cggggctgct cacgatcgat gcgcagcgca tcgccgtcac gccgaagggg cgcatgttcg tgcgcgcgat cggcatggtg ttcgacgcgt atctcggccg cagcgccgcg gcgtcttatt cgaaattgat ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa