BupsA.17298.a

Oxygen-independent coproporphyrinogen III oxidase

CENTER ID: BupsA.17298.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17298.a.A1.GE32582 Full length( BupsA.17298.a ) 1 464
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3257
RefSeq: YP_334494.1
UniProt: Q3JPK9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQPIPSEAL FRPDLLAKYT ANGPRYTSYP TAPQFRDNFD PADYIRAAAD PGASASDLSL YFHIPFCATA CFYCGCNKIA TRNRKRARPY LDQLKHEIAL HAALFDPERT VTQLHWGGGT PTFLSNAETT ELMAATREYF TLASDRTGEF SIEIDPRTAS PATLVHLRTI GFNRVSLGVQ DFDPDVQRAI NRVQPLKMTS DLVRAARATG YHSIGIDLIY GLPHQTVSSF ARTLDTIVEL EPDRLSVFGY AHLPHLFKMQ RQIDAAALPP PATRIALLGL AIERLTRAGY VYIGMDHFAR PGDELVRAQR NGTLQRNFQG YSTRADADLI GIGASSIGKV GDVYAQNAKD LPRYGAALAD QRLPIARGVK LSADDRLRRD VITHLMCNLV LPFSHVEAAY GIRFANKFAR ELDTLRGFER DGLLSIGADR LTIHPAGRLY VRNIAMVFDA YLSGASEHRY SKTV
NT Sequence
ATGACGCAGC CGATTCCGTC TGAGGCGCTG TTTCGCCCCG ACCTGCTCGC CAAGTACACG GCGAACGGCC CTCGCTATAC GTCCTACCCC ACCGCCCCTC AGTTCCGCGA CAACTTCGAT CCCGCCGATT ACATCCGCGC GGCGGCCGAT CCCGGCGCGT CGGCGAGCGA TCTGTCGCTC TATTTCCACA TTCCGTTCTG CGCGACCGCC TGCTTCTATT GCGGCTGCAA CAAGATCGCG ACGCGCAACC GCAAGCGCGC GCGCCCGTAT CTCGACCAGT TGAAGCACGA GATCGCGCTG CACGCCGCGC TCTTCGATCC GGAGCGGACC GTCACGCAGC TCCACTGGGG CGGCGGCACG CCGACTTTCC TGTCGAACGC CGAGACGACC GAGCTGATGG CGGCCACGCG CGAGTACTTC ACGCTCGCGT CGGATCGCAC CGGAGAATTC TCGATCGAGA TCGATCCGCG CACGGCGTCG CCCGCGACGC TCGTCCATCT GCGCACGATC GGCTTCAATC GCGTAAGCCT CGGCGTGCAG GACTTCGACC CCGACGTGCA ACGCGCGATC AATCGCGTGC AGCCGCTCAA GATGACGTCC GACCTCGTGC GCGCCGCGCG CGCGACCGGC TATCACTCGA TCGGCATCGA TCTCATCTAC GGGCTGCCGC ACCAGACGGT ATCGAGCTTC GCGCGCACGC TCGACACGAT CGTCGAGCTC GAGCCCGACC GGCTCTCCGT GTTCGGCTAC GCGCACCTGC CGCACCTGTT CAAGATGCAG CGGCAGATCG ACGCCGCCGC GCTGCCGCCG CCCGCGACGC GCATCGCGCT GCTCGGCCTC GCGATCGAGC GGCTCACGCG CGCGGGCTAT GTGTACATCG GCATGGACCA TTTCGCGCGG CCGGGCGACG AGCTCGTCCG CGCGCAGCGC AACGGCACGC TGCAGCGCAA CTTCCAGGGC TACAGCACGC GGGCGGACGC CGATCTGATC GGCATCGGCG CGTCCTCGAT CGGCAAGGTC GGCGACGTCT ACGCGCAGAA CGCGAAGGAT CTGCCGCGTT ACGGCGCGGC GCTCGCCGAC CAGCGTCTGC CGATCGCGCG CGGCGTGAAG CTCTCGGCCG ACGACCGGCT GCGGCGCGAC GTGATCACGC ACCTGATGTG CAACCTCGTG CTGCCGTTCT CGCACGTCGA GGCCGCGTAC GGCATCCGCT TCGCGAACAA GTTCGCGCGC GAGCTCGACA CGCTGCGCGG CTTCGAGCGC GACGGCCTGC TGTCGATCGG CGCCGATCGT CTGACGATTC ATCCGGCCGG GCGCCTCTAC GTGCGCAACA TCGCGATGGT GTTCGACGCG TACCTGTCGG GCGCGTCCGA ACACCGCTAT TCGAAGACGG TC
Details for BupsA.17298.a.A1.GE32582
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQPIPSEA LFRPDLLAKY TANGPRYTSY PTAPQFRDNF DPADYIRAAA DPGASASDLS LYFHIPFCAT ACFYCGCNKI ATRNRKRARP YLDQLKHEIA LHAALFDPER TVTQLHWGGG TPTFLSNAET TELMAATREY FTLASDRTGE FSIEIDPRTA SPATLVHLRT IGFNRVSLGV QDFDPDVQRA INRVQPLKMT SDLVRAARAT GYHSIGIDLI YGLPHQTVSS FARTLDTIVE LEPDRLSVFX YXHLPHLXKM QRQIDAAALP PPATRIALLX XAIERLXRAG XVXXGMXHFA XXXXLVXXXX RCXXXXLXXX GXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
gnttnnnttt aanaaggaga nataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgacgcagcc gattccgtct gaggcgctgt ttcgccccga cctgctcgcc aagtacacgg cgaacggccc tcgctatacg tcctacccca ccgcccctca gttccgcgac aacttcgatc ccgccgatta catccgcgcg gcggccgatc ccggcgcgtc ggcgagcgat ctgtcgctct atttccacat tccgttctgc gcgaccgcct gcttctattg cggctgcaac aagatcgcga cgcgcaaccg caagcgcgcg cgcccgtatc tcgaccagtt gaagcacgag atcgcgctgc acgccgcgct cttcgatccg gagcggaccg tcacgcagct ccactggggc ggcggcacgc cgactttcct gtcgaacgcc gagacgaccg agctgatggc ggccacgcgc gagtacttca cgctcgcgtc ggatcgcacc ggagaattct cgatcgagat cgatccgcgc acggcgtcgc ccgcgacgct cgtccatctg cgcacgatcg gcttcaatcg cgtaagcctc ggcgtgcagg acttcgaccc cgacgtgcaa cgcgcgatca atcgcgtgca gccgctcaag atgacgtccg acctcgtgcg cgccgcgcgc gcgaccggct atcactcgat cggcatcgat ctcatctacg ggctgccgca ccagacggta tcgagcttcg cgcgcacgct cgacacgatc gtcgagctcg agcccgaccg gctctccgtg ttcgnctacn cgcacctgcc gcacctgntc aagatgcagc ggcagatcga cgccgccgcg ctgccgccgc ccgcgacgcg catcgcgctg ctcngncncg cgatcgagcg gctcangcgc gcgggctang tnnacntcgg natggnncat ttcgcnnnnn nnnacnagct cgtnnnnngc ancgnacgct gcancgnacn nnggctacng nnnncnggnn gnngccnann nnatntngnn nnnnnnnnan nnnnnngnnn gnnannnntn nnnnncnnna nnnnnnnnnn nnnnnnnngn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQPIPSEA LFRPDLLAKY TANGPRYTSY PTAPQFRDNF DPADYIRAAA DPGASASDLS LYFHIPFCAT ACFYCGCNKI ATRNRKRARP YLDQLKHEIA LHAALFDPER TVTQLHWGGG TPTFLSNAET TELMAATREY FTLASDRTGE FSIEIDPRTA SPATLVHLRT IGFNRVSLGV QDFDPDVQRA INRVQPLKMT SDLVRAARAT GYHSIGIDLI YGLPHQTVSS FARTLDTIVE LEPDRLSVFG YAHLPHLFKM QRQIDAAALP PPATRIALLG LAIERLTRAG YVYIGMDHFA RPGDELVRAQ RNGTLQRNFQ GYSTRADADL IGIGASSIGK VGDVYAQNAK DLPRYGAALA DQRLPIARGV KLSADDRLRR DVITHLMCNL VLPFSHVEAA YGIRFANKFA RELDTLRGFE RDGLLSIGAD RLTIHPAGRL YVRNIAMVFD AYLSGASEHR YSKTV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcagccg attccgtctg aggcgctgtt tcgccccgac ctgctcgcca agtacacggc gaacggccct cgctatacgt cctaccccac cgcccctcag ttccgcgaca acttcgatcc cgccgattac atccgcgcgg cggccgatcc cggcgcgtcg gcgagcgatc tgtcgctcta tttccacatt ccgttctgcg cgaccgcctg cttctattgc ggctgcaaca agatcgcgac gcgcaaccgc aagcgcgcgc gcccgtatct cgaccagttg aagcacgaga tcgcgctgca cgccgcgctc ttcgatccgg agcggaccgt cacgcagctc cactggggcg gcggcacgcc gactttcctg tcgaacgccg agacgaccga gctgatggcg gccacgcgcg agtacttcac gctcgcgtcg gatcgcaccg gagaattctc gatcgagatc gatccgcgca cggcgtcgcc cgcgacgctc gtccatctgc gcacgatcgg cttcaatcgc gtaagcctcg gcgtgcagga cttcgacccc gacgtgcaac gcgcgatcaa tcgcgtgcag ccgctcaaga tgacgtccga cctcgtgcgc gccgcgcgcg cgaccggcta tcactcgatc ggcatcgatc tcatctacgg gctgccgcac cagacggtat cgagcttcgc gcgcacgctc gacacgatcg tcgagctcga gcccgaccgg ctctccgtgt tcggctacgc gcacctgccg cacctgttca agatgcagcg gcagatcgac gccgccgcgc tgccgccgcc cgcgacgcgc atcgcgctgc tcggcctcgc gatcgagcgg ctcacgcgcg cgggctatgt gtacatcggc atggaccatt tcgcgcggcc gggcgacgag ctcgtccgcg cgcagcgcaa cggcacgctg cagcgcaact tccagggcta cagcacgcgg gcggacgccg atctgatcgg catcggcgcg tcctcgatcg gcaaggtcgg cgacgtctac gcgcagaacg cgaaggatct gccgcgttac ggcgcggcgc tcgccgacca gcgtctgccg atcgcgcgcg gcgtgaagct ctcggccgac gaccggctgc ggcgcgacgt gatcacgcac ctgatgtgca acctcgtgct gccgttctcg cacgtcgagg ccgcgtacgg catccgcttc gcgaacaagt tcgcgcgcga gctcgacacg ctgcgcggct tcgagcgcga cggcctgctg tcgatcggcg ccgatcgtct gacgattcat ccggccgggc gcctctacgt gcgcaacatc gcgatggtgt tcgacgcgta cctgtcgggc gcgtccgaac accgctattc gaagacggtc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa