BupsA.17295.b

Glycosyl transferase, group 1 family protein

CENTER ID: BupsA.17295.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17295.b.A1.GE31060 Full length( BupsA.17295.b ) 1 400
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.80
RefSeq: YP_331500.1
UniProt: Q3JY53

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRNARSGDV RRVPPRLPAG RRSPLTGAKL APPLGEFLPL RNGETLMRIA QIAPLYEAVP PKLYGGTERV VSYLTEALVE MGHDVTLFAS GDSVTSARLE AAWPRALRLD PSIRDAMAPH MRLLEQVARA AHTFDILHFH LDYLPFPLLS RLDAPFVTTL HGRLDLPELQ PVFDAFPNAP VVSISDSQRT PLPQAGWAGT VYHGLPDTLL TPQAGRKPEY LAFLGRICPE KRVDTAIRIA AQSGLPLKIA AKVDKVDEDY FKAEIEPLLD SAHVEFIGEI NEAQKPAFLS GAKALLFPID WPEPFGLVMI EAMACGTPVV AFNRGSVPEV IDDGLTGFIV EDVQGAVGAL HRIDELSRDA IRAQFEQRFS SHAMARRYID IYETLRGATK QPQWQRVAAG
NT Sequence
ATGAATCGGA ACGCCCGGTC CGGCGACGTT CGCCGCGTAC CGCCCCGTCT GCCCGCCGGC AGGCGCTCAC CGCTTACCGG AGCGAAGCTC GCACCGCCCC TCGGCGAGTT CCTCCCTCTT CGCAACGGAG AAACGCTCAT GCGCATCGCC CAAATCGCCC CGCTCTACGA AGCCGTCCCG CCCAAACTCT ACGGGGGCAC CGAGCGGGTC GTGTCCTACC TGACCGAGGC GCTCGTCGAA ATGGGGCACG ACGTCACGCT GTTCGCAAGC GGGGATTCCG TCACGTCGGC GCGGCTGGAA GCCGCATGGC CGCGCGCGCT GCGCCTCGAT CCGTCGATCC GCGACGCGAT GGCGCCCCAT ATGCGCTTGC TCGAGCAGGT CGCACGGGCC GCGCACACGT TCGACATCCT GCACTTCCAC CTCGACTACC TGCCGTTCCC ACTGCTGTCG CGCCTCGACG CGCCGTTCGT CACGACGTTG CACGGCCGCC TCGATCTGCC GGAGCTGCAG CCCGTGTTCG ACGCGTTCCC GAACGCGCCC GTCGTGTCGA TCTCGGATTC GCAGCGCACG CCGCTGCCGC AAGCCGGATG GGCCGGCACC GTCTACCACG GGCTGCCCGA CACGCTGCTC ACGCCGCAAG CCGGCCGCAA GCCTGAATAT CTCGCGTTTC TCGGCCGCAT CTGCCCGGAA AAACGCGTCG ACACCGCAAT CCGGATCGCC GCGCAAAGCG GCCTGCCGCT GAAGATTGCT GCGAAAGTCG ACAAGGTCGA CGAGGATTAT TTCAAGGCCG AGATCGAACC GCTGCTCGAT TCCGCGCACG TCGAGTTCAT CGGCGAGATC AACGAAGCAC AGAAGCCCGC CTTCCTGTCG GGCGCGAAGG CGCTGCTGTT TCCGATCGAT TGGCCCGAGC CGTTCGGCCT CGTGATGATC GAGGCGATGG CATGCGGCAC GCCCGTCGTC GCGTTCAACC GCGGCTCCGT GCCGGAAGTC ATCGATGACG GCCTGACGGG CTTCATCGTC GAGGACGTAC AGGGCGCGGT GGGCGCGCTG CACCGGATCG ACGAATTGTC ACGCGATGCA ATTCGTGCGC AATTCGAGCA ACGTTTCAGC TCGCATGCAA TGGCGCGGCG CTATATCGAC ATCTATGAAA CGCTGCGCGG CGCAACCAAG CAACCGCAAT GGCAGCGCGT AGCGGCGGGT
Details for BupsA.17295.b.A1.GE31060
HARVESTED ON: 12/14/2010
SEQUENCED ON: 1/13/2011
EXPECTED MW: 46kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 8
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSHDAQRTS VGFSYGT
Validated NT Sequence
gnnnnnnctn tntannnnaa ggagnnatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgagc cacgacgcgc aacgaacaag cgtcggattc agctatggca cttaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgcnnn nnnntaacta gcaannntct acgaagccgt cccgcccaaa ctctacgggg gcaccgagcg ggtcgtgtcc tacctgaccg aggcgctcgt cgaaatgggg cacgacgtca cgctgttcgc aagcggggat tccgtcacgt cggcgcggct ggaagccgca tggccgcgcg cgctgcgcct cgatccgtcg atccgcgacg cgatggcgcc ccatatgcgc ttgctcgagc aggtcgcacg ggccgcgcac acgttcgaca tcctgcactt ccacctcgac tacctgccgt tcccactgct gtcgcgcctc gacgcgccgt tcgtcacgac gttgcacggc cgcctcgatc tgccggagct gcagcccgtg ttcgacgcgt tcccgaacgc gcccgtcgtg tcgatctcgg attcgcagcg cacgccgctg ccgcaagccg gatgggccgg caccgtctac cacgggctgc ccgacacgct gctcacgccg cangccggcc gcaagcctga atatctcgcg tttctcggcc gcatctgccc ggaaaaacgc gtcgacaccg caatccggat cgccgcgcaa agcggcctgc cgctgaagan tgctgcgaaa gtcgacaang tcgannanga ttatttcaag ggccganatc gaaccgctgc tcgattccgc gcacgtcgag ttcatcngnn nagatcaacg aagcncagaa nnccgncttc ctgtcgggcg cgangcnctg ctgtttnnga tcgatnnnnc cgnnncgntc ggnctcntga tgatcgannn nannggcatg nnnnacgncc gncnnnnnnn tcnnccnnnn ccnnnnngna nntcancgan nnnnnnnnnn nnnnnnnnnt tnntcnnnnn ngnacagnnn nnnnnnnnnn nnnnncnnnc nnnnnnnnnn ngnnnntnnn nnnnnnnnnn nnnnnnannn nnntt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNRNARSGD VRRVPPRLPA GRRSPLTGAK LAPPLGEFLP LRNGETLMRI AQIAPLYEAV PPKLYGGTER VVSYLTEALV EMGHDVTLFA SGDSVTSARL EAAWPRALRL DPSIRDAMAP HMRLLEQVAR AAHTFDILHF HLDYLPFPLL SRLDAPFVTT LHGRLDLPEL QPVFDAFPNA PVVSISDSQR TPLPQAGWAG TVYHGLPDTL LTPQAGRKPE YLAFLGRICP EKRVDTAIRI AAQSGLPLKI AAKVDKVDED YFKAEIEPLL DSAHVEFIGE INEAQKPAFL SGAKALLFPI DWPEPFGLVM IEAMACGTPV VAFNRGSVPE VIDDGLTGFI VEDVQGAVGA LHRIDELSRD AIRAQFEQRF SSHAMARRYI DIYETLRGAT KQPQWQRVAA G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatcggaac gcccggtccg gcgacgttcg ccgcgtaccg ccccgtctgc ccgccggcag gcgctcaccg cttaccggag cgaagctcgc accgcccctc ggcgagttcc tccctcttcg caacggagaa acgctcatgc gcatcgccca aatcgccccg ctctacgaag ccgtcccgcc caaactctac gggggcaccg agcgggtcgt gtcctacctg accgaggcgc tcgtcgaaat ggggcacgac gtcacgctgt tcgcaagcgg ggattccgtc acgtcggcgc ggctggaagc cgcatggccg cgcgcgctgc gcctcgatcc gtcgatccgc gacgcgatgg cgccccatat gcgcttgctc gagcaggtcg cacgggccgc gcacacgttc gacatcctgc acttccacct cgactacctg ccgttcccac tgctgtcgcg cctcgacgcg ccgttcgtca cgacgttgca cggccgcctc gatctgccgg agctgcagcc cgtgttcgac gcgttcccga acgcgcccgt cgtgtcgatc tcggattcgc agcgcacgcc gctgccgcaa gccggatggg ccggcaccgt ctaccacggg ctgcccgaca cgctgctcac gccgcaagcc ggccgcaagc ctgaatatct cgcgtttctc ggccgcatct gcccggaaaa acgcgtcgac accgcaatcc ggatcgccgc gcaaagcggc ctgccgctga agattgctgc gaaagtcgac aaggtcgacg aggattattt caaggccgag atcgaaccgc tgctcgattc cgcgcacgtc gagttcatcg gcgagatcaa cgaagcacag aagcccgcct tcctgtcggg cgcgaaggcg ctgctgtttc cgatcgattg gcccgagccg ttcggcctcg tgatgatcga ggcgatggca tgcggcacgc ccgtcgtcgc gttcaaccgc ggctccgtgc cggaagtcat cgatgacggc ctgacgggct tcatcgtcga ggacgtacag ggcgcggtgg gcgcgctgca ccggatcgac gaattgtcac gcgatgcaat tcgtgcgcaa ttcgagcaac gtttcagctc gcatgcaatg gcgcggcgct atatcgacat ctatgaaacg ctgcgcggcg caaccaagca accgcaatgg cagcgcgtag cggcgggtta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a