SSGCID: Target Page

BupsA.17294.d

Outative extracellular ligand binding protein

CENTER ID:	BupsA.17294.d
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17294.d.A2.GE31058	Mature protein( BupsA.17294.d )		27	380

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17294.d.A2.PS00973	Mature protein( BupsA.17294.d )		27	380

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.50
RefSeq:	YP_331468.1
UniProt:	Q3JY85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNIKMQKLLP ISAAAMLLAA AATNAAADQV VKIGHVAPLT GGIAHLGKDN ENGARLAVEE INAKGLTIGG QKVTLQLDAQ DDAADPRTAT QVAQKLVDDK VVAVVGHLNS GTSIPASKIY SDAGIVQISP SATNPAYTQQ GFKTTYRVVA TDAQQGPALA NYARSKGVKS VAVVDDSTAY GQGLANEFEK KAKALGLKVV SHDATNDKAV DFRAILTKIK GENPDAVMYG GMDATGGPFA KQAKQLGLRA KIFAGDGVCT EKLADLAGDA TDNVVCSEAG ASLEKMPGGA AFKAKYEKRF GQPIQIYAPF TYDAVYIIVD AMKRANSTDP AKIVAAMPKT NYTGVIGTTT FDSKGDLQHG VISLYDYKGG KKTFLDEVKM

NT Sequence

ATGAATATCA AGATGCAAAA GCTGCTGCCG ATCAGCGCGG CGGCTATGCT GTTGGCCGCA GCCGCCACGA ACGCTGCCGC CGACCAGGTC GTCAAGATCG GCCATGTCGC ACCGTTGACG GGCGGGATCG CCCACCTTGG TAAGGACAAC GAAAATGGTG CGCGCCTCGC GGTCGAGGAG ATCAACGCGA AGGGACTCAC GATCGGCGGC CAGAAGGTGA CGCTCCAGCT CGATGCACAG GACGACGCGG CCGACCCGCG CACCGCGACG CAGGTCGCGC AGAAGCTCGT CGACGACAAG GTCGTCGCGG TCGTCGGCCA CCTGAACTCG GGTACCTCGA TTCCGGCCTC GAAGATCTAC AGCGATGCGG GCATCGTGCA GATCTCGCCG TCGGCGACGA ACCCGGCTTA CACGCAGCAA GGTTTCAAGA CGACCTACCG CGTCGTCGCG ACCGACGCGC AGCAGGGCCC GGCGCTCGCG AACTACGCGC GCTCGAAGGG CGTGAAGAGC GTCGCCGTCG TCGACGATTC GACGGCCTAC GGCCAGGGCC TCGCGAACGA ATTCGAGAAG AAGGCGAAGG CGCTCGGCCT GAAGGTGGTG TCGCACGACG CGACGAACGA CAAGGCGGTC GACTTCCGCG CGATCCTGAC GAAGATCAAG GGTGAAAATC CGGACGCCGT GATGTACGGC GGCATGGACG CGACGGGCGG CCCGTTCGCG AAGCAGGCGA AGCAGCTCGG CCTGCGCGCG AAGATCTTCG CGGGCGACGG CGTCTGCACG GAAAAGCTCG CGGACCTCGC GGGCGATGCG ACCGACAACG TCGTGTGCTC GGAGGCGGGC GCGTCGCTCG AGAAGATGCC GGGCGGCGCG GCATTCAAGG CGAAGTACGA GAAGCGTTTC GGCCAGCCGA TCCAGATCTA CGCGCCGTTC ACGTATGACG CGGTGTACAT CATCGTCGAC GCGATGAAGC GCGCGAACTC GACGGATCCG GCGAAGATCG TCGCGGCGAT GCCGAAGACG AACTACACCG GCGTGATCGG CACGACGACC TTCGATTCGA AGGGCGACCT GCAGCACGGC GTGATCTCGC TGTACGACTA CAAGGGCGGC AAGAAGACGT TCCTGGATGA AGTGAAGATG

Details for BupsA.17294.d.A2.GE31058

HARVESTED ON:	12/14/2010
SEQUENCED ON:	12/20/2010
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	40

Validated AA Sequence

MYGGMDATGG PFAKQAKQLG LRAKIFAGDG VCTEKLADLA GDATDNVVCS EAGASLEKMP GGAAFKAKYE KRFGQPIQIY APFTYDAVYI IVDAMKRANS TDPAKIVAAM PKTNYTGVIG TTTFDSKGDL QHGVISLYDY KGGKKTFLDE V

Validated NT Sequence

tcacttcatc caggaacgtc ttcttgccgc ccttgtagtc gtacagcgag atcacgccgt gctgcaggtc gcccttcgaa tcgaaggtcg tcgtgccgat cacgccggtg tagttcgtct tcggcatcgc cgcgacgatc ttcgccggat ccgtcgagtt cgcgcgcttc atcgcgtcga cgatgatgta caccgcgtca tacgtgaacg gcgcgtagat ctggatcggc tggccgaaac gcttctcgta cttcgccttg aatgccgcgc cgcccggcat cttctcgagc gacgcgcccg cctccgagca cacgacgttg tcggtcgcat cgcccgcgag gtccgcgagc ttttccgtgc agacgccgtc gcccgcgaag atcttcgcgc gcaggccgag ctgcttcgcc tgcttcgcga acgggccgcc cgtcgcgtcc atgccgccgt acatcacggc gtccggattt tcacccttga tcttcgtcag gatcgcgcgg aagtcgaccg ccttgtcgtt cgtcgcgtcg tgcgacacca ccttcaggcc gagcgccttc gccttcttct cgaattcgtt cgcgaggccc tggccgtagg ccgtcgaatc gtcgacgacg gcgacgctct tcacgccctt cgagcgcgcg tagttcgcga gcgccgggcc ctgctgcgcg tcggtcgcga cgacgcggta ggtcgtcttg aaaccttgct gcgtgtaagc cgggttcgtc gccgacggcg agatctgcac gatgcccgca tcgctgtaga tcttcgaggc cggaatcnag gtacccgant tcnggtggcc gacgaccgcg acgaccntgt cgtcnacgag cttctgcgcg acntgcgtcg cggngcnnnn nngg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMADQVVKIG HVAPLTGGIA HLGKDNENGA RLAVEEINAK GLTIGGQKVT LQLDAQDDAA DPRTATQVAQ KLVDDKVVAV VGHLNSGTSI PASKIYSDAG IVQISPSATN PAYTQQGFKT TYRVVATDAQ QGPALANYAR SKGVKSVAVV DDSTAYGQGL ANEFEKKAKA LGLKVVSHDA TNDKAVDFRA ILTKIKGENP DAVMYGGMDA TGGPFAKQAK QLGLRAKIFA GDGVCTEKLA DLAGDATDNV VCSEAGASLE KMPGGAAFKA KYEKRFGQPI QIYAPFTYDA VYIIVDAMKR ANSTDPAKIV AAMPKTNYTG VIGTTTFDSK GDLQHGVISL YDYKGGKKTF LDEVKM

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgaccag gtcgtcaaga tcggccatgt cgcaccgttg acgggcggga tcgcccacct tggtaaggac aacgaaaatg gtgcgcgcct cgcggtcgag gagatcaacg cgaagggact cacgatcggc ggccagaagg tgacgctcca gctcgatgca caggacgacg cggccgaccc gcgcaccgcg acgcaggtcg cgcagaagct cgtcgacgac aaggtcgtcg cggtcgtcgg ccacctgaac tcgggtacct cgattccggc ctcgaagatc tacagcgatg cgggcatcgt gcagatctcg ccgtcggcga cgaacccggc ttacacgcag caaggtttca agacgaccta ccgcgtcgtc gcgaccgacg cgcagcaggg cccggcgctc gcgaactacg cgcgctcgaa gggcgtgaag agcgtcgccg tcgtcgacga ttcgacggcc tacggccagg gcctcgcgaa cgaattcgag aagaaggcga aggcgctcgg cctgaaggtg gtgtcgcacg acgcgacgaa cgacaaggcg gtcgacttcc gcgcgatcct gacgaagatc aagggtgaaa atccggacgc cgtgatgtac ggcggcatgg acgcgacggg cggcccgttc gcgaagcagg cgaagcagct cggcctgcgc gcgaagatct tcgcgggcga cggcgtctgc acggaaaagc tcgcggacct cgcgggcgat gcgaccgaca acgtcgtgtg ctcggaggcg ggcgcgtcgc tcgagaagat gccgggcggc gcggcattca aggcgaagta cgagaagcgt ttcggccagc cgatccagat ctacgcgccg ttcacgtatg acgcggtgta catcatcgtc gacgcgatga agcgcgcgaa ctcgacggat ccggcgaaga tcgtcgcggc gatgccgaag acgaactaca ccggcgtgat cggcacgacg accttcgatt cgaagggcga cctgcagcac ggcgtgatct cgctgtacga ctacaagggc ggcaagaaga cgttcctgga tgaagtgaag atgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BupsA.17294.d.A2.PS00973

PURIFICATION DATe:	2/11/2011
CONCENTRATION:	9.02mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	40

Protocol Notes

notes unavailable

Validated AA Sequence

MYGGMDATGG PFAKQAKQLG LRAKIFAGDG VCTEKLADLA GDATDNVVCS EAGASLEKMP GGAAFKAKYE KRFGQPIQIY APFTYDAVYI IVDAMKRANS TDPAKIVAAM PKTNYTGVIG TTTFDSKGDL QHGVISLYDY KGGKKTFLDE V

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGACCAG GTCGTCAAGA TCGGCCATGT CGCACCGTTG ACGGGCGGGA TCGCCCACCT TGGTAAGGAC AACGAAAATG GTGCGCGCCT CGCGGTCGAG GAGATCAACG CGAAGGGACT CACGATCGGC GGCCAGAAGG TGACGCTCCA GCTCGATGCA CAGGACGACG CGGCCGACCC GCGCACCGCG ACGCAGGTCG CGCAGAAGCT CGTCGACGAC AAGGTCGTCG CGGTCGTCGG CCACCTGAAC TCGGGTACCT CGATTCCGGC CTCGAAGATC TACAGCGATG CGGGCATCGT GCAGATCTCG CCGTCGGCGA CGAACCCGGC TTACACGCAG CAAGGTTTCA AGACGACCTA CCGCGTCGTC GCGACCGACG CGCAGCAGGG CCCGGCGCTC GCGAACTACG CGCGCTCGAA GGGCGTGAAG AGCGTCGCCG TCGTCGACGA TTCGACGGCC TACGGCCAGG GCCTCGCGAA CGAATTCGAG AAGAAGGCGA AGGCGCTCGG CCTGAAGGTG GTGTCGCACG ACGCGACGAA CGACAAGGCG GTCGACTTCC GCGCGATCCT GACGAAGATC AAGGGTGAAA ATCCGGACGC CGTGATGTAC GGCGGCATGG ACGCGACGGG CGGCCCGTTC GCGAAGCAGG CGAAGCAGCT CGGCCTGCGC GCGAAGATCT TCGCGGGCGA CGGCGTCTGC ACGGAAAAGC TCGCGGACCT CGCGGGCGAT GCGACCGACA ACGTCGTGTG CTCGGAGGCG GGCGCGTCGC TCGAGAAGAT GCCGGGCGGC GCGGCATTCA AGGCGAAGTA CGAGAAGCGT TTCGGCCAGC CGATCCAGAT CTACGCGCCG TTCACGTATG ACGCGGTGTA CATCATCGTC GACGCGATGA AGCGCGCGAA CTCGACGGAT CCGGCGAAGA TCGTCGCGGC GATGCCGAAG ACGAACTACA CCGGCGTGAT CGGCACGACG ACCTTCGATT CGAAGGGCGA CCTGCAGCAC GGCGTGATCT CGCTGTACGA CTACAAGGGC GGCAAGAAGA CGTTCCTGGA TGAAGTGAAG ATGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA