BupsA.17294.c

Outative extracellular ligand binding protein

CENTER ID: BupsA.17294.c
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17294.c.A2.GE32577 Mature protein( BupsA.17294.c ) 25 379
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17294.c.A2.PW32497 Mature protein( BupsA.17294.c ) 25 379

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.159
RefSeq: YP_331581.1
UniProt: Q3JXX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQHTMKKLAG ATFVAVMSLA GTAHADDVKI GFAAPMTGAQ AHYGKDMQNG IVLAIEDINA TKPTIGGKPV KFVLDTQDDQ ADPRTGTTVA QKLVDDGIKG MLGHFNSGTT IPASRIYANA GIPQIAMATA PEYTTQGYKT TFRMMTSDTQ QGSVAGTFAV KDLGMKKIAI VDDRTAYGQG LADQFEKAAK AAGATIVDRE FTNDKAVDFK AILTKLKATK PDLVYYGGAD SQAAPMAKQM KSLGVTAPLM GGEMVHTPTF LKIAGDAAEG SIASLAGLPL AEMPGGKAYA DKYKKRFGED VQTYSPYAYD GAMAMFSAMK KANSTDPAKY LPLLAKTDMA GVTSTHIAYD AKGDLRNGGI TMYKVEKGEW KPLKSIGGK
NT Sequence
ATGCAACACA CGATGAAAAA GCTGGCAGGC GCGACGTTCG TCGCGGTCAT GTCGCTCGCG GGGACGGCGC ACGCGGATGA CGTCAAGATC GGCTTTGCCG CGCCGATGAC GGGCGCGCAG GCGCACTACG GCAAGGATAT GCAGAACGGG ATCGTGCTCG CGATCGAGGA CATCAACGCG ACGAAGCCGA CGATCGGCGG CAAGCCGGTG AAGTTCGTGC TCGACACGCA GGACGATCAG GCCGACCCGC GCACCGGCAC GACGGTCGCG CAGAAGCTCG TCGACGACGG CATCAAGGGC ATGCTCGGCC ACTTCAACTC GGGCACGACG ATTCCGGCTT CGCGCATCTA CGCGAACGCG GGCATCCCGC AGATCGCGAT GGCGACGGCG CCCGAGTACA CGACGCAGGG CTACAAGACG ACCTTCCGGA TGATGACGTC CGACACGCAG CAGGGCTCGG TCGCGGGCAC GTTCGCGGTG AAGGATCTCG GCATGAAGAA GATCGCGATC GTCGACGATC GCACCGCTTA CGGCCAGGGC CTTGCCGACC AGTTCGAGAA GGCGGCGAAG GCGGCGGGCG CGACGATCGT CGATCGTGAA TTCACGAACG ACAAGGCTGT CGACTTCAAG GCGATCCTGA CGAAGCTCAA GGCGACGAAG CCGGACCTCG TCTACTACGG CGGCGCGGAT TCGCAGGCCG CGCCGATGGC CAAGCAGATG AAGTCGCTCG GCGTCACGGC GCCGCTGATG GGCGGCGAGA TGGTGCACAC GCCGACCTTC CTGAAGATCG CGGGCGACGC GGCCGAAGGC TCGATCGCTT CGCTCGCCGG CCTGCCGCTC GCCGAAATGC CCGGCGGCAA GGCGTACGCG GACAAGTACA AGAAGCGCTT CGGCGAAGAC GTGCAGACGT ACTCGCCGTA TGCGTACGAC GGCGCGATGG CGATGTTCAG CGCGATGAAG AAGGCGAACT CGACGGACCC GGCGAAGTAT CTGCCGCTGC TCGCGAAGAC CGACATGGCG GGCGTGACGT CGACGCACAT CGCGTATGAC GCGAAGGGCG ACCTGAGGAA CGGCGGCATC ACGATGTACA AGGTCGAGAA GGGCGAATGG AAGCCCCTGA AGAGCATCGG CGGCAAG
Details for BupsA.17294.c.A2.GE32577
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 39kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MADDVKIGFA APMTGAQAHY GKDMQNGIVL AIEDINATKP TIGGKPVKFV LDTQDDQADP RTGTTVAQKL VDDGIKGMLG HFNSGTTIPA SRIYANAGIP QIAMATAPEY TTQGYKTTFR MMTSDTQQGS VAGTFAVKDL GMKKIAIVDD RTAYGQGLAD QFEKAAKAAG ATIVDREFTN DKAVDFKAIL TKLKATKPDL VYYGGADSQA APMAKQMKSL GVTAPLMGGE MVHTPTFLKI AGDAAEGSIA SLAGLPLAEM PGGKAYADKY KKRFGEDVQT YSPYAYDGAM AMFSAMKKAN STDPAKYLPL LAKTDMAGVT STHIAYDAKG DLRNGGITMY KVEKGEWKPL KSIG
Validated NT Sequence
ccgccgatgc tcttcagggg cttccattcg cccttctcga ccttgtacat cgtgatgccg ccgttcctca ggtcgccctt cgcgtcatac gcgatgtgcg tcgacgtcac gcccgccatg tcggtcttcg cgagcagcgg cagatacttc gccgggtccg tcgagttcgc cttcttcatc gcgctgaaca tcgccatcgc gccgtcgtac gcatacggcg agtacgtctg cacgtcttcg ccgaagcgct tcttgtactt gtccgcgtac gccttgccgc cgggcatttc ggcgagcggc aggccggcga gcgaagcgat cgagccttcg gccgcgtcgc ccgcgatctt caggaaggtc ggcgtgtgca ccatctcgcc gcccatcagc ggcgccgtga cgccgagcga cttcatctgc ttggccatcg gcgcggcctg cgaatccgcg ccgccgtagt agacgaggtc cggcttcgtc gccttgagct tcgtcaggat cgccttgaag tcgacagcct tgtcgttcgt gaattcacga tcgacgatcg tcgcgcccgc cgccttcgcc gccttctcga actggtcggc aaggccctgg ccgtaagcgg tgcgatcgtc gacgatcgcg atcttcttca tgccgagatc cttcaccgcg aacgtgcccg cgaccgagcc ctgctgcgtg tcggacgtca tcatccggaa ggtcgtcttg tagccctgcg tcgtgtactc gggcgccgtc gccatcgcga tctgcgggat gcccgcgttc gcgtagatgc gcgaagccgg aatcgtcgtg cccgagttga agtggccgag catgcccttg atgccgtcgt cgacgagctt ctgcgcgacc gtcgtgccgg tgcgcgggtc ggcctgatcg tcctgcgtgt cgagcacgaa cttcaccggc ttgccgccga tcgtcggctt cgtcgcgttg atgtcctcga tcgcgagcac gatcccgttc tgcatatcct tgccgtagtg cgcctgcgcg cccgtcatcg gcgcggcaaa gccgatcttg acgtcatccg ccatcgaacc aggaccctgg gtctgagctt ccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADDVKIGF AAPMTGAQAH YGKDMQNGIV LAIEDINATK PTIGGKPVKF VLDTQDDQAD PRTGTTVAQK LVDDGIKGML GHFNSGTTIP ASRIYANAGI PQIAMATAPE YTTQGYKTTF RMMTSDTQQG SVAGTFAVKD LGMKKIAIVD DRTAYGQGLA DQFEKAAKAA GATIVDREFT NDKAVDFKAI LTKLKATKPD LVYYGGADSQ AAPMAKQMKS LGVTAPLMGG EMVHTPTFLK IAGDAAEGSI ASLAGLPLAE MPGGKAYADK YKKRFGEDVQ TYSPYAYDGA MAMFSAMKKA NSTDPAKYLP LLAKTDMAGV TSTHIAYDAK GDLRNGGITM YKVEKGEWKP LKSIGGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggatgac gtcaagatcg gctttgccgc gccgatgacg ggcgcgcagg cgcactacgg caaggatatg cagaacggga tcgtgctcgc gatcgaggac atcaacgcga cgaagccgac gatcggcggc aagccggtga agttcgtgct cgacacgcag gacgatcagg ccgacccgcg caccggcacg acggtcgcgc agaagctcgt cgacgacggc atcaagggca tgctcggcca cttcaactcg ggcacgacga ttccggcttc gcgcatctac gcgaacgcgg gcatcccgca gatcgcgatg gcgacggcgc ccgagtacac gacgcagggc tacaagacga ccttccggat gatgacgtcc gacacgcagc agggctcggt cgcgggcacg ttcgcggtga aggatctcgg catgaagaag atcgcgatcg tcgacgatcg caccgcttac ggccagggcc ttgccgacca gttcgagaag gcggcgaagg cggcgggcgc gacgatcgtc gatcgtgaat tcacgaacga caaggctgtc gacttcaagg cgatcctgac gaagctcaag gcgacgaagc cggacctcgt ctactacggc ggcgcggatt cgcaggccgc gccgatggcc aagcagatga agtcgctcgg cgtcacggcg ccgctgatgg gcggcgagat ggtgcacacg ccgaccttcc tgaagatcgc gggcgacgcg gccgaaggct cgatcgcttc gctcgccggc ctgccgctcg ccgaaatgcc cggcggcaag gcgtacgcgg acaagtacaa gaagcgcttc ggcgaagacg tgcagacgta ctcgccgtat gcgtacgacg gcgcgatggc gatgttcagc gcgatgaaga aggcgaactc gacggacccg gcgaagtatc tgccgctgct cgcgaagacc gacatggcgg gcgtgacgtc gacgcacatc gcgtatgacg cgaagggcga cctgaggaac ggcggcatca cgatgtacaa ggtcgagaag ggcgaatgga agcccctgaa gagcatcggc ggcaagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BupsA.17294.c.A2.PW32497
PURIFICATION DATe: 7/2/2011
CONCENTRATION: 33.12mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MADDVKIGFA APMTGAQAHY GKDMQNGIVL AIEDINATKP TIGGKPVKFV LDTQDDQADP RTGTTVAQKL VDDGIKGMLG HFNSGTTIPA SRIYANAGIP QIAMATAPEY TTQGYKTTFR MMTSDTQQGS VAGTFAVKDL GMKKIAIVDD RTAYGQGLAD QFEKAAKAAG ATIVDREFTN DKAVDFKAIL TKLKATKPDL VYYGGADSQA APMAKQMKSL GVTAPLMGGE MVHTPTFLKI AGDAAEGSIA SLAGLPLAEM PGGKAYADKY KKRFGEDVQT YSPYAYDGAM AMFSAMKKAN STDPAKYLPL LAKTDMAGVT STHIAYDAKG DLRNGGITMY KVEKGEWKPL KSIG
Validated NT Sequence
ccgccgatgc tcttcagggg cttccattcg cccttctcga ccttgtacat cgtgatgccg ccgttcctca ggtcgccctt cgcgtcatac gcgatgtgcg tcgacgtcac gcccgccatg tcggtcttcg cgagcagcgg cagatacttc gccgggtccg tcgagttcgc cttcttcatc gcgctgaaca tcgccatcgc gccgtcgtac gcatacggcg agtacgtctg cacgtcttcg ccgaagcgct tcttgtactt gtccgcgtac gccttgccgc cgggcatttc ggcgagcggc aggccggcga gcgaagcgat cgagccttcg gccgcgtcgc ccgcgatctt caggaaggtc ggcgtgtgca ccatctcgcc gcccatcagc ggcgccgtga cgccgagcga cttcatctgc ttggccatcg gcgcggcctg cgaatccgcg ccgccgtagt agacgaggtc cggcttcgtc gccttgagct tcgtcaggat cgccttgaag tcgacagcct tgtcgttcgt gaattcacga tcgacgatcg tcgcgcccgc cgccttcgcc gccttctcga actggtcggc aaggccctgg ccgtaagcgg tgcgatcgtc gacgatcgcg atcttcttca tgccgagatc cttcaccgcg aacgtgcccg cgaccgagcc ctgctgcgtg tcggacgtca tcatccggaa ggtcgtcttg tagccctgcg tcgtgtactc gggcgccgtc gccatcgcga tctgcgggat gcccgcgttc gcgtagatgc gcgaagccgg aatcgtcgtg cccgagttga agtggccgag catgcccttg atgccgtcgt cgacgagctt ctgcgcgacc gtcgtgccgg tgcgcgggtc ggcctgatcg tcctgcgtgt cgagcacgaa cttcaccggc ttgccgccga tcgtcggctt cgtcgcgttg atgtcctcga tcgcgagcac gatcccgttc tgcatatcct tgccgtagtg cgcctgcgcg cccgtcatcg gcgcggcaaa gccgatcttg acgtcatccg ccatcgaacc aggaccctgg gtctgagctt ccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADDVKIGF AAPMTGAQAH YGKDMQNGIV LAIEDINATK PTIGGKPVKF VLDTQDDQAD PRTGTTVAQK LVDDGIKGML GHFNSGTTIP ASRIYANAGI PQIAMATAPE YTTQGYKTTF RMMTSDTQQG SVAGTFAVKD LGMKKIAIVD DRTAYGQGLA DQFEKAAKAA GATIVDREFT NDKAVDFKAI LTKLKATKPD LVYYGGADSQ AAPMAKQMKS LGVTAPLMGG EMVHTPTFLK IAGDAAEGSI ASLAGLPLAE MPGGKAYADK YKKRFGEDVQ TYSPYAYDGA MAMFSAMKKA NSTDPAKYLP LLAKTDMAGV TSTHIAYDAK GDLRNGGITM YKVEKGEWKP LKSIGGK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGATGAC GTCAAGATCG GCTTTGCCGC GCCGATGACG GGCGCGCAGG CGCACTACGG CAAGGATATG CAGAACGGGA TCGTGCTCGC GATCGAGGAC ATCAACGCGA CGAAGCCGAC GATCGGCGGC AAGCCGGTGA AGTTCGTGCT CGACACGCAG GACGATCAGG CCGACCCGCG CACCGGCACG ACGGTCGCGC AGAAGCTCGT CGACGACGGC ATCAAGGGCA TGCTCGGCCA CTTCAACTCG GGCACGACGA TTCCGGCTTC GCGCATCTAC GCGAACGCGG GCATCCCGCA GATCGCGATG GCGACGGCGC CCGAGTACAC GACGCAGGGC TACAAGACGA CCTTCCGGAT GATGACGTCC GACACGCAGC AGGGCTCGGT CGCGGGCACG TTCGCGGTGA AGGATCTCGG CATGAAGAAG ATCGCGATCG TCGACGATCG CACCGCTTAC GGCCAGGGCC TTGCCGACCA GTTCGAGAAG GCGGCGAAGG CGGCGGGCGC GACGATCGTC GATCGTGAAT TCACGAACGA CAAGGCTGTC GACTTCAAGG CGATCCTGAC GAAGCTCAAG GCGACGAAGC CGGACCTCGT CTACTACGGC GGCGCGGATT CGCAGGCCGC GCCGATGGCC AAGCAGATGA AGTCGCTCGG CGTCACGGCG CCGCTGATGG GCGGCGAGAT GGTGCACACG CCGACCTTCC TGAAGATCGC GGGCGACGCG GCCGAAGGCT CGATCGCTTC GCTCGCCGGC CTGCCGCTCG CCGAAATGCC CGGCGGCAAG GCGTACGCGG ACAAGTACAA GAAGCGCTTC GGCGAAGACG TGCAGACGTA CTCGCCGTAT GCGTACGACG GCGCGATGGC GATGTTCAGC GCGATGAAGA AGGCGAACTC GACGGACCCG GCGAAGTATC TGCCGCTGCT CGCGAAGACC GACATGGCGG GCGTGACGTC GACGCACATC GCGTATGACG CGAAGGGCGA CCTGAGGAAC GGCGGCATCA CGATGTACAA GGTCGAGAAG GGCGAATGGA AGCCCCTGAA GAGCATCGGC GGCAAGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA