SSGCID: Target Page

BupsA.17294.a

Outative extracellular ligand binding protein

CENTER ID:	BupsA.17294.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17294.a.A2.GE32575	Mature protein( BupsA.17294.a )		19	372

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17294.a.A2.PW32495	Mature protein( BupsA.17294.a )		19	372

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.6587
RefSeq:	YP_337539.1
UniProt:	Q3JFW4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPISAAAMVM AAASTHALAD QVVKIGSAEP LTGGIAHLGK DNENGARLAV EEINAKGLTI GGQKVTLQLD AQDDAADPRT ATQVAQKLVD GKVVAVIGHL NSGTSIPASK IYSDAGILQI SPSATNPAYT QQGFKTTYRV VATDAQQGPA LADYAKQKGI KTVAVVDDST AYGQGLANEF EKKAKALGLK VVSHDATNDK AVDFRAILTK IKGTNPDAIM YGGMDATGGP FAKQAKQLGL RAKIFAGDGV CTEQLPALAG NAAENVVCSQ AGAALEKMPG GATFQAKYEK RFNQPIRFDA PFTYDAVYII VDAMKRANST DPAKILAVMP KTNYTGVIGT TVFDSKGDLK HGVISLYDFK GGKKTFLDQV TM

NT Sequence

TTGCCGATCA GCGCGGCCGC GATGGTGATG GCCGCGGCCT CGACGCATGC GCTCGCCGAT CAGGTCGTGA AGATCGGCAG CGCCGAGCCC TTGACGGGCG GCATCGCCCA CCTCGGCAAG GACAACGAGA ACGGCGCGCG TCTCGCGGTC GAGGAGATCA ATGCGAAGGG CCTCACGATC GGCGGCCAGA AGGTCACGCT CCAGCTCGAC GCGCAGGACG ACGCGGCCGA CCCGCGCACC GCGACGCAGG TCGCGCAGAA GCTCGTCGAC GGCAAGGTCG TCGCGGTGAT CGGCCACCTG AATTCGGGAA CGTCGATTCC GGCCTCGAAG ATCTACAGCG ATGCCGGCAT CCTGCAGATC TCGCCGTCGG CGACGAACCC GGCCTACACG CAACAAGGCT TCAAGACGAC CTACCGCGTC GTCGCGACCG ACGCGCAGCA AGGCCCGGCG CTCGCCGACT ACGCGAAGCA GAAGGGCATC AAGACGGTGG CCGTCGTCGA CGATTCGACG GCCTACGGCC AGGGCCTCGC GAACGAATTC GAGAAGAAGG CGAAGGCGCT CGGCCTGAAG GTGGTGTCGC ACGACGCGAC CAACGACAAG GCGGTCGATT TCCGCGCGAT CCTGACGAAG ATCAAGGGTA CGAACCCGGA CGCGATCATG TACGGCGGCA TGGACGCGAC GGGCGGCCCG TTCGCGAAGC AGGCGAAGCA GCTCGGCCTG CGCGCGAAGA TCTTCGCGGG CGACGGCGTG TGCACCGAGC AGTTGCCGGC GCTCGCCGGC AACGCGGCCG AGAACGTCGT GTGCTCGCAG GCGGGCGCGG CGCTCGAGAA GATGCCGGGC GGCGCGACGT TCCAGGCCAA GTACGAGAAG CGCTTCAACC AGCCGATCCG CTTCGACGCG CCGTTCACGT ATGACGCGGT GTACATCATC GTCGACGCGA TGAAGCGCGC GAACTCGACG GACCCGGCGA AGATCCTCGC GGTGATGCCG AAGACGAACT ACACCGGCGT GATCGGCACG ACGGTCTTCG ATTCGAAGGG TGACCTGAAG CACGGCGTGA TTTCGCTGTA CGACTTCAAG GGCGGCAAGA AGACCTTCCT CGATCAGGTG ACGATG

Details for BupsA.17294.a.A2.GE32575

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Validated AA Sequence

MADQVVKIGS AEPLTGGIAH LGKDNENGAR LAVEEINAKG LTIGGQKVTL QLDAQDDAAD PRTATQVAQK LVDGKVVAVI GHLNSGTSIP ASKIYSDAGI LQISPSATNP AYTQQGFKTT YRVVATDAQQ GPALADYAKQ KGIKTVAVVD DSTAYGQGLA NEFEKKAKAL GLKVVSHDAT NDKAVDFRAI LTKIKGTNPD AIMYGGMDAT GGPFAKQAKQ LGLRAKIFAG DGVCTEQLPA LAGNAAENVV CSQAGAALEK MPGGATFQAK YEKRFNQPIR FDAPFTYDAV YIIVDAMKRA NSTDPAKILA VMPKTNYTGV IGTTVFDSKG DLKHGVISLY DFKGGKKT

Validated NT Sequence

aggtcttctt gccgcccttg aagtcgtaca gcgaaatcac gccgtgcttc aggtcaccct tcgaatcgaa gaccgtcgtg ccgatcacgc cggtgtagtt cgtcttcggc atcaccgcga ggatcttcgc cgggtccgtc gagttcgcgc gcttcatcgc gtcgacgatg atgtacaccg cgtcatacgt gaacggcgcg tcgaagcgga tcggctggtt gaagcgcttc tcgtacttgg cctggaacgt cgcgccgccc ggcatcttct cgagcgccgc gcccgcctgc gagcacacga cgttctcggc cgcgttgccg gcgagcgccg gcaactgctc ggtgcacacg ccgtcgcccg cgaagatctt cgcgcgcagg ccgagctgct tcgcctgctt cgcgaacggg ccgcccgtcg cgtccatgcc gccgtacatg atcgcgtccg ggttcgtacc cttgatcttc gtcaggatcg cgcggaaatc gaccgccttg tcgttggtcg cgtcgtgcga caccaccttc aggccgagcg ccttcgcctt cttctcgaat tcgttcgcga ggccctggcc gtaggccgtc gaatcgtcga cgacggccac cgtcttgatg cccttctgct tcgcgtagtc ggcgagcgcc gggccttgct gcgcgtcggt cgcgacgacg cggtaggtcg tcttgaagcc ttgttgcgtg taggccgggt tcgtcgccga cggcgagatc tgcaggatgc cggcatcgct gtagatcttc gaggccggaa tcgacgttcc cgaattcagg tggccgatca ccgcgacgac cttgccgtcg acgagcttct gcgcgacctg cgtcgcggtg cgcgggtcgg ccgcgtcgtc ctgcgcgtcg agctggagcg tgaccttctg gccgccgatc gtgaggccct tcgcattgat ctcctcgacc gcgagacgcg cgccgttctc gttgtccttg ccgaggtggg cgatgccgcc cgtcaagggc tcggcgctgc cgatcttcac gacctgatcg gccatcgaac caggaccctg ggtctgagct tccagggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMADQVVKIG SAEPLTGGIA HLGKDNENGA RLAVEEINAK GLTIGGQKVT LQLDAQDDAA DPRTATQVAQ KLVDGKVVAV IGHLNSGTSI PASKIYSDAG ILQISPSATN PAYTQQGFKT TYRVVATDAQ QGPALADYAK QKGIKTVAVV DDSTAYGQGL ANEFEKKAKA LGLKVVSHDA TNDKAVDFRA ILTKIKGTNP DAIMYGGMDA TGGPFAKQAK QLGLRAKIFA GDGVCTEQLP ALAGNAAENV VCSQAGAALE KMPGGATFQA KYEKRFNQPI RFDAPFTYDA VYIIVDAMKR ANSTDPAKIL AVMPKTNYTG VIGTTVFDSK GDLKHGVISL YDFKGGKKTF LDQVTM

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgatcag gtcgtgaaga tcggcagcgc cgagcccttg acgggcggca tcgcccacct cggcaaggac aacgagaacg gcgcgcgtct cgcggtcgag gagatcaatg cgaagggcct cacgatcggc ggccagaagg tcacgctcca gctcgacgcg caggacgacg cggccgaccc gcgcaccgcg acgcaggtcg cgcagaagct cgtcgacggc aaggtcgtcg cggtgatcgg ccacctgaat tcgggaacgt cgattccggc ctcgaagatc tacagcgatg ccggcatcct gcagatctcg ccgtcggcga cgaacccggc ctacacgcaa caaggcttca agacgaccta ccgcgtcgtc gcgaccgacg cgcagcaagg cccggcgctc gccgactacg cgaagcagaa gggcatcaag acggtggccg tcgtcgacga ttcgacggcc tacggccagg gcctcgcgaa cgaattcgag aagaaggcga aggcgctcgg cctgaaggtg gtgtcgcacg acgcgaccaa cgacaaggcg gtcgatttcc gcgcgatcct gacgaagatc aagggtacga acccggacgc gatcatgtac ggcggcatgg acgcgacggg cggcccgttc gcgaagcagg cgaagcagct cggcctgcgc gcgaagatct tcgcgggcga cggcgtgtgc accgagcagt tgccggcgct cgccggcaac gcggccgaga acgtcgtgtg ctcgcaggcg ggcgcggcgc tcgagaagat gccgggcggc gcgacgttcc aggccaagta cgagaagcgc ttcaaccagc cgatccgctt cgacgcgccg ttcacgtatg acgcggtgta catcatcgtc gacgcgatga agcgcgcgaa ctcgacggac ccggcgaaga tcctcgcggt gatgccgaag acgaactaca ccggcgtgat cggcacgacg gtcttcgatt cgaagggtga cctgaagcac ggcgtgattt cgctgtacga cttcaagggc ggcaagaaga ccttcctcga tcaggtgacg atgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BupsA.17294.a.A2.PW32495

PURIFICATION DATe:	7/2/2011
CONCENTRATION:	26.23mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	32
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGATCAG GTCGTGAAGA TCGGCAGCGC CGAGCCCTTG ACGGGCGGCA TCGCCCACCT CGGCAAGGAC AACGAGAACG GCGCGCGTCT CGCGGTCGAG GAGATCAATG CGAAGGGCCT CACGATCGGC GGCCAGAAGG TCACGCTCCA GCTCGACGCG CAGGACGACG CGGCCGACCC GCGCACCGCG ACGCAGGTCG CGCAGAAGCT CGTCGACGGC AAGGTCGTCG CGGTGATCGG CCACCTGAAT TCGGGAACGT CGATTCCGGC CTCGAAGATC TACAGCGATG CCGGCATCCT GCAGATCTCG CCGTCGGCGA CGAACCCGGC CTACACGCAA CAAGGCTTCA AGACGACCTA CCGCGTCGTC GCGACCGACG CGCAGCAAGG CCCGGCGCTC GCCGACTACG CGAAGCAGAA GGGCATCAAG ACGGTGGCCG TCGTCGACGA TTCGACGGCC TACGGCCAGG GCCTCGCGAA CGAATTCGAG AAGAAGGCGA AGGCGCTCGG CCTGAAGGTG GTGTCGCACG ACGCGACCAA CGACAAGGCG GTCGATTTCC GCGCGATCCT GACGAAGATC AAGGGTACGA ACCCGGACGC GATCATGTAC GGCGGCATGG ACGCGACGGG CGGCCCGTTC GCGAAGCAGG CGAAGCAGCT CGGCCTGCGC GCGAAGATCT TCGCGGGCGA CGGCGTGTGC ACCGAGCAGT TGCCGGCGCT CGCCGGCAAC GCGGCCGAGA ACGTCGTGTG CTCGCAGGCG GGCGCGGCGC TCGAGAAGAT GCCGGGCGGC GCGACGTTCC AGGCCAAGTA CGAGAAGCGC TTCAACCAGC CGATCCGCTT CGACGCGCCG TTCACGTATG ACGCGGTGTA CATCATCGTC GACGCGATGA AGCGCGCGAA CTCGACGGAC CCGGCGAAGA TCCTCGCGGT GATGCCGAAG ACGAACTACA CCGGCGTGAT CGGCACGACG GTCTTCGATT CGAAGGGTGA CCTGAAGCAC GGCGTGATTT CGCTGTACGA CTTCAAGGGC GGCAAGAAGA CCTTCCTCGA TCAGGTGACG ATGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA