BupsA.17293.d

ISBma2, transposase

CENTER ID: BupsA.17293.d
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17293.d.A1.GE32574 Full length( BupsA.17293.d ) 1 487
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17293.d.A1.PS01355 Full length( BupsA.17293.d ) 1 487

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2866
RefSeq: YP_334126.1
UniProt: Q3JQM7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKTPMPTQH ELEMVTLEEL VPKDHLLRQI DAAVDFEFIR AKVAHLYCAD NGRPALDPVV MFKLLFIGYL FGVRSERQLM REVQVNVAYR WFARFRLTDK VPDASTFSQN RRRRFTDTTV YQEIFDEIVR QAIKRGLVDG RVLYTDSTHL KANANKGKFD VVKLEQTPAA YTEALNAAVD ADRAAHGKKP LDRDDDEPPS SKDTKISRTD PDSGYMVRDD KPKGFFYLDH RTVDAKHAII TDTHVTPASV HDSQPYLDRL DRQRERFEFK VEAVGLDAGY FTPAVCQGLE ERGIAGVMGY RTPNHKPGMF YKRQFKYDAY RNEYVCPQGQ ALPYSTTNRL GYREYKSNAQ ICGRCPVRSQ CTNSAIAVKV VTRHVWERAK ERVDARRLTE WGQRIYARRK QTVERSFADA KQLHGHRYAR MRGLRKVAEQ CLLAAAAQNI KKIAMLLARK RKKGPAGPDW RFVRMLLRLV SGLRCSFDYP LAANPQS
NT Sequence
ATGCTGAAGA CGCCCATGCC CACGCAGCAC GAACTCGAGA TGGTGACGCT CGAGGAACTC GTGCCGAAGG ACCACCTGCT GCGCCAGATC GATGCGGCGG TGGATTTCGA GTTCATCCGC GCGAAGGTGG CGCATCTGTA TTGCGCGGAC AACGGGCGGC CGGCGCTCGA TCCCGTGGTG ATGTTCAAGC TGTTGTTCAT CGGCTACCTG TTCGGGGTGC GCAGCGAGCG GCAACTGATG CGTGAGGTCC AGGTCAACGT CGCCTATCGC TGGTTCGCCC GGTTCCGGCT GACCGACAAG GTGCCGGATG CGTCAACGTT CTCGCAGAAT CGCCGCCGAC GCTTCACGGA CACGACGGTG TATCAGGAGA TCTTCGACGA GATCGTGCGG CAGGCGATCA AGCGCGGGCT GGTCGACGGT CGGGTGCTGT ACACGGACAG CACGCACCTG AAGGCGAACG CGAACAAGGG CAAGTTCGAT GTGGTGAAGC TGGAGCAGAC GCCGGCGGCC TACACGGAGG CATTGAACGC GGCAGTGGAT GCGGACCGGG CCGCGCATGG CAAGAAGCCG CTGGATCGCG ACGACGATGA GCCGCCGTCG AGCAAGGACA CCAAGATCAG CCGGACCGAT CCGGACAGCG GCTACATGGT GCGGGACGAC AAGCCGAAGG GGTTCTTCTA TCTGGACCAC CGCACGGTGG ATGCCAAGCA CGCGATCATC ACCGATACGC ATGTGACGCC GGCCTCGGTG CATGACAGCC AGCCGTATCT GGATCGGCTG GATCGCCAGC GCGAGCGCTT TGAGTTCAAG GTCGAGGCGG TGGGGCTGGA TGCGGGCTAC TTCACGCCGG CGGTGTGCCA GGGGCTGGAG GAGCGAGGGA TTGCCGGGGT GATGGGCTAT CGCACGCCGA ACCACAAGCC GGGCATGTTC TACAAACGGC AGTTCAAGTA CGACGCGTAT CGCAACGAAT ACGTGTGCCC GCAGGGGCAG GCCCTGCCGT ACAGCACGAC CAATCGGCTC GGCTATCGGG AATACAAATC CAATGCGCAG ATCTGCGGGC GCTGCCCGGT ACGATCGCAG TGCACGAACA GTGCGATCGC GGTGAAGGTG GTAACGCGCC ACGTGTGGGA GCGCGCCAAG GAGCGGGTGG ACGCGCGGCG CTTGACCGAA TGGGGCCAAC GCATTTACGC GCGGCGCAAG CAGACGGTGG AGCGCAGCTT CGCCGATGCC AAGCAGTTGC ATGGGCACCG TTATGCCCGT ATGCGTGGGC TACGCAAGGT GGCCGAGCAG TGCTTGCTGG CCGCGGCGGC ACAGAACATC AAGAAGATTG CGATGCTGCT GGCGCGGAAG CGGAAAAAGG GGCCAGCGGG TCCCGATTGG CGCTTCGTGC GCATGCTGCT GCGTCTGGTG AGCGGTTTGC GCTGCAGCTT CGACTACCCG CTCGCGGCGA ACCCGCAATC C
Details for BupsA.17293.d.A1.GE32574
HARVESTED ON: 5/17/2011
SEQUENCED ON: 10/13/2014
EXPECTED MW: 58kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLKTPMPTQ HELEMVTLEE LVPKDHLLRQ IDAAVDFEFI RAKVAHLYCA DNGRPALDPV VMFKLLFIGY LFGVRSERQL MREVQVNVAY RWFARFRLTD KVPDASTFSQ NRRRRFTDTT VYQEIFDEIV RQAIKRGLVD GRVLYTDSTH LKANANKGKF DVVKLEQTPA AYTEALNAAV DADRAAHGKK PLDRDDDEPP SSKDTKISRT DPDSGYMVRD DKPKGFFYLD HRTVDAKHAI ITDTHVTPAS VHDSQPYLDR LDRQRERFEF KVEAVGLDAG YFTPAVCQGL EERGIAGVMG YRTPNHKPGM FYKRQFKYDA YRNEYVCPQG QALPYSTTNR LGYREYKSNA QICGRCPVRS QCTNSAIAVK VVTRHVWERA KERVDARRLT EWGQRIYARR KQTVERSFAD AKQLHGHRYA RMRGLRKVAE QCLLAAAAQN IKKIAMLLAR KRKKGPAGPD WRFVRMLLRL VSGLRCSFDY PLAAN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctga agacgcccat gcccacgcag cacgaactcg agatggtgac gctcgaggaa ctcgtgccga aggaccacct gctgcgccag atcgatgcgg cggtggattt cgagttcatc cgcgcgaagg tggcgcatct gtattgcgcg gacaacgggc ggccggcgct cgatcccgtg gtgatgttca agctgttgtt catcggctac ctgttcgggg tgcgcagcga gcggcaactg atgcgtgagg tccaggtcaa cgtcgcctat cgctggttcg cccggttccg gctgaccgac aaggtgccgg atgcgtcaac gttctcgcag aatcgccgcc gacgcttcac ggacacgacg gtgtatcagg agatcttcga cgagatcgtg cggcaggcga tcaagcgcgg gctggtcgac ggtcgggtgc tgtacacgga cagcacgcac ctgaaggcga acgcgaacaa gggcaagttc gatgtggtga agctggagca gacgccggcg gcctacacgg aggcattgaa cgcggcagtg gatgcggacc gggccgcgca tggcaagaag ccgctggatc gcgacgacga tgagccgccg tcgagcaagg acaccaagat cagccggacc gatccggaca gcggctacat ggtgcgggac gacaagccga aggggttctt ctatctggac caccgcacgg tggatgccaa gcacgcgatc atcaccgata cgcatgtgac gccggcctcg gtgcatgaca gccagccgta tctggatcgg ctggatcgcc agcgcgagcg ctttgagttc aaggtcgagg cggtggggct ggatgcgggc tacttcacgc cggcggtgtg ccaggggctg gaggagcgag ggattgccgg ggtgatgggc tatcgcacgc cgaaccacaa gccgggcatg ttctacaaac ggcagttcaa gtacgacgcg tatcgcaacg aatacgtgtg cccgcagggg caggccctgc cgtacagcac gaccaatcgg ctcggctatc gggaatacaa atccaatgcg cagatctgcg ggcgctgccc ggtacgatcg cagtgcacga acagtgcgat cgcggtgaag gtggtaacgc gccacgtgtg ggagcgcgcc aaggagcggg tggacgcgcg gcgcttgacc gaatggggcc aacgcattta cgcgcggcgc aagcagacgg tggagcgcag cttcgccgat gccaagcagc tgcatgggca ccgttatgcc cgtatgcgtg ggctacgcaa ggtggccgag cagtgcttgc tggccgcggc ggcacagaac atcaagaaga ttgcgatgct gctggcgcgg aagcggaaaa aggggccagc gggtcccgat tggcgcttcg tgcgcatgct gctgcgtctg gtgagcggtt tgcgctgcag cttcgactac ccgctcgcgg cgaacccg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKTPMPTQ HELEMVTLEE LVPKDHLLRQ IDAAVDFEFI RAKVAHLYCA DNGRPALDPV VMFKLLFIGY LFGVRSERQL MREVQVNVAY RWFARFRLTD KVPDASTFSQ NRRRRFTDTT VYQEIFDEIV RQAIKRGLVD GRVLYTDSTH LKANANKGKF DVVKLEQTPA AYTEALNAAV DADRAAHGKK PLDRDDDEPP SSKDTKISRT DPDSGYMVRD DKPKGFFYLD HRTVDAKHAI ITDTHVTPAS VHDSQPYLDR LDRQRERFEF KVEAVGLDAG YFTPAVCQGL EERGIAGVMG YRTPNHKPGM FYKRQFKYDA YRNEYVCPQG QALPYSTTNR LGYREYKSNA QICGRCPVRS QCTNSAIAVK VVTRHVWERA KERVDARRLT EWGQRIYARR KQTVERSFAD AKQLHGHRYA RMRGLRKVAE QCLLAAAAQN IKKIAMLLAR KRKKGPAGPD WRFVRMLLRL VSGLRCSFDY PLAANPQS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaagacg cccatgccca cgcagcacga actcgagatg gtgacgctcg aggaactcgt gccgaaggac cacctgctgc gccagatcga tgcggcggtg gatttcgagt tcatccgcgc gaaggtggcg catctgtatt gcgcggacaa cgggcggccg gcgctcgatc ccgtggtgat gttcaagctg ttgttcatcg gctacctgtt cggggtgcgc agcgagcggc aactgatgcg tgaggtccag gtcaacgtcg cctatcgctg gttcgcccgg ttccggctga ccgacaaggt gccggatgcg tcaacgttct cgcagaatcg ccgccgacgc ttcacggaca cgacggtgta tcaggagatc ttcgacgaga tcgtgcggca ggcgatcaag cgcgggctgg tcgacggtcg ggtgctgtac acggacagca cgcacctgaa ggcgaacgcg aacaagggca agttcgatgt ggtgaagctg gagcagacgc cggcggccta cacggaggca ttgaacgcgg cagtggatgc ggaccgggcc gcgcatggca agaagccgct ggatcgcgac gacgatgagc cgccgtcgag caaggacacc aagatcagcc ggaccgatcc ggacagcggc tacatggtgc gggacgacaa gccgaagggg ttcttctatc tggaccaccg cacggtggat gccaagcacg cgatcatcac cgatacgcat gtgacgccgg cctcggtgca tgacagccag ccgtatctgg atcggctgga tcgccagcgc gagcgctttg agttcaaggt cgaggcggtg gggctggatg cgggctactt cacgccggcg gtgtgccagg ggctggagga gcgagggatt gccggggtga tgggctatcg cacgccgaac cacaagccgg gcatgttcta caaacggcag ttcaagtacg acgcgtatcg caacgaatac gtgtgcccgc aggggcaggc cctgccgtac agcacgacca atcggctcgg ctatcgggaa tacaaatcca atgcgcagat ctgcgggcgc tgcccggtac gatcgcagtg cacgaacagt gcgatcgcgg tgaaggtggt aacgcgccac gtgtgggagc gcgccaagga gcgggtggac gcgcggcgct tgaccgaatg gggccaacgc atttacgcgc ggcgcaagca gacggtggag cgcagcttcg ccgatgccaa gcagttgcat gggcaccgtt atgcccgtat gcgtgggcta cgcaaggtgg ccgagcagtg cttgctggcc gcggcggcac agaacatcaa gaagattgcg atgctgctgg cgcggaagcg gaaaaagggg ccagcgggtc ccgattggcg cttcgtgcgc atgctgctgc gtctggtgag cggtttgcgc tgcagcttcg actacccgct cgcggcgaac ccgcaatcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.17293.d.A1.PS01355
PURIFICATION DATe: 1/18/2012
CONCENTRATION: 24.56mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLKTPMPTQ HELEMVTLEE LVPKDHLLRQ IDAAVDFEFI RAKVAHLYCA DNGRPALDPV VMFKLLFIGY LFGVRSERQL MREVQVNVAY RWFARFRLTD KVPDASTFSQ NRRRRFTDTT VYQEIFDEIV RQAIKRGLVD GRVLYTDSTH LKANANKGKF DVVKLEQTPA AYTEALNAAV DADRAAHGKK PLDRDDDEPP SSKDTKISRT DPDSGYMVRD DKPKGFFYLD HRTVDAKHAI ITDTHVTPAS VHDSQPYLDR LDRQRERFEF KVEAVGLDAG YFTPAVCQGL EERGIAGVMG YRTPNHKPGM FYKRQFKYDA YRNEYVCPQG QALPYSTTNR LGYREYKSNA QICGRCPVRS QCTNSAIAVK VVTRHVWERA KERVDARRLT EWGQRIYARR KQTVERSFAD AKQLHGHRYA RMRGLRKVAE QCLLAAAAQN IKKIAMLLAR KRKKGPAGPD WRFVRMLLRL VSGLRCSFDY PLAAN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctga agacgcccat gcccacgcag cacgaactcg agatggtgac gctcgaggaa ctcgtgccga aggaccacct gctgcgccag atcgatgcgg cggtggattt cgagttcatc cgcgcgaagg tggcgcatct gtattgcgcg gacaacgggc ggccggcgct cgatcccgtg gtgatgttca agctgttgtt catcggctac ctgttcgggg tgcgcagcga gcggcaactg atgcgtgagg tccaggtcaa cgtcgcctat cgctggttcg cccggttccg gctgaccgac aaggtgccgg atgcgtcaac gttctcgcag aatcgccgcc gacgcttcac ggacacgacg gtgtatcagg agatcttcga cgagatcgtg cggcaggcga tcaagcgcgg gctggtcgac ggtcgggtgc tgtacacgga cagcacgcac ctgaaggcga acgcgaacaa gggcaagttc gatgtggtga agctggagca gacgccggcg gcctacacgg aggcattgaa cgcggcagtg gatgcggacc gggccgcgca tggcaagaag ccgctggatc gcgacgacga tgagccgccg tcgagcaagg acaccaagat cagccggacc gatccggaca gcggctacat ggtgcgggac gacaagccga aggggttctt ctatctggac caccgcacgg tggatgccaa gcacgcgatc atcaccgata cgcatgtgac gccggcctcg gtgcatgaca gccagccgta tctggatcgg ctggatcgcc agcgcgagcg ctttgagttc aaggtcgagg cggtggggct ggatgcgggc tacttcacgc cggcggtgtg ccaggggctg gaggagcgag ggattgccgg ggtgatgggc tatcgcacgc cgaaccacaa gccgggcatg ttctacaaac ggcagttcaa gtacgacgcg tatcgcaacg aatacgtgtg cccgcagggg caggccctgc cgtacagcac gaccaatcgg ctcggctatc gggaatacaa atccaatgcg cagatctgcg ggcgctgccc ggtacgatcg cagtgcacga acagtgcgat cgcggtgaag gtggtaacgc gccacgtgtg ggagcgcgcc aaggagcggg tggacgcgcg gcgcttgacc gaatggggcc aacgcattta cgcgcggcgc aagcagacgg tggagcgcag cttcgccgat gccaagcagc tgcatgggca ccgttatgcc cgtatgcgtg ggctacgcaa ggtggccgag cagtgcttgc tggccgcggc ggcacagaac atcaagaaga ttgcgatgct gctggcgcgg aagcggaaaa aggggccagc gggtcccgat tggcgcttcg tgcgcatgct gctgcgtctg gtgagcggtt tgcgctgcag cttcgactac ccgctcgcgg cgaacccg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKTPMPTQ HELEMVTLEE LVPKDHLLRQ IDAAVDFEFI RAKVAHLYCA DNGRPALDPV VMFKLLFIGY LFGVRSERQL MREVQVNVAY RWFARFRLTD KVPDASTFSQ NRRRRFTDTT VYQEIFDEIV RQAIKRGLVD GRVLYTDSTH LKANANKGKF DVVKLEQTPA AYTEALNAAV DADRAAHGKK PLDRDDDEPP SSKDTKISRT DPDSGYMVRD DKPKGFFYLD HRTVDAKHAI ITDTHVTPAS VHDSQPYLDR LDRQRERFEF KVEAVGLDAG YFTPAVCQGL EERGIAGVMG YRTPNHKPGM FYKRQFKYDA YRNEYVCPQG QALPYSTTNR LGYREYKSNA QICGRCPVRS QCTNSAIAVK VVTRHVWERA KERVDARRLT EWGQRIYARR KQTVERSFAD AKQLHGHRYA RMRGLRKVAE QCLLAAAAQN IKKIAMLLAR KRKKGPAGPD WRFVRMLLRL VSGLRCSFDY PLAANPQS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTGAAGACG CCCATGCCCA CGCAGCACGA ACTCGAGATG GTGACGCTCG AGGAACTCGT GCCGAAGGAC CACCTGCTGC GCCAGATCGA TGCGGCGGTG GATTTCGAGT TCATCCGCGC GAAGGTGGCG CATCTGTATT GCGCGGACAA CGGGCGGCCG GCGCTCGATC CCGTGGTGAT GTTCAAGCTG TTGTTCATCG GCTACCTGTT CGGGGTGCGC AGCGAGCGGC AACTGATGCG TGAGGTCCAG GTCAACGTCG CCTATCGCTG GTTCGCCCGG TTCCGGCTGA CCGACAAGGT GCCGGATGCG TCAACGTTCT CGCAGAATCG CCGCCGACGC TTCACGGACA CGACGGTGTA TCAGGAGATC TTCGACGAGA TCGTGCGGCA GGCGATCAAG CGCGGGCTGG TCGACGGTCG GGTGCTGTAC ACGGACAGCA CGCACCTGAA GGCGAACGCG AACAAGGGCA AGTTCGATGT GGTGAAGCTG GAGCAGACGC CGGCGGCCTA CACGGAGGCA TTGAACGCGG CAGTGGATGC GGACCGGGCC GCGCATGGCA AGAAGCCGCT GGATCGCGAC GACGATGAGC CGCCGTCGAG CAAGGACACC AAGATCAGCC GGACCGATCC GGACAGCGGC TACATGGTGC GGGACGACAA GCCGAAGGGG TTCTTCTATC TGGACCACCG CACGGTGGAT GCCAAGCACG CGATCATCAC CGATACGCAT GTGACGCCGG CCTCGGTGCA TGACAGCCAG CCGTATCTGG ATCGGCTGGA TCGCCAGCGC GAGCGCTTTG AGTTCAAGGT CGAGGCGGTG GGGCTGGATG CGGGCTACTT CACGCCGGCG GTGTGCCAGG GGCTGGAGGA GCGAGGGATT GCCGGGGTGA TGGGCTATCG CACGCCGAAC CACAAGCCGG GCATGTTCTA CAAACGGCAG TTCAAGTACG ACGCGTATCG CAACGAATAC GTGTGCCCGC AGGGGCAGGC CCTGCCGTAC AGCACGACCA ATCGGCTCGG CTATCGGGAA TACAAATCCA ATGCGCAGAT CTGCGGGCGC TGCCCGGTAC GATCGCAGTG CACGAACAGT GCGATCGCGG TGAAGGTGGT AACGCGCCAC GTGTGGGAGC GCGCCAAGGA GCGGGTGGAC GCGCGGCGCT TGACCGAATG GGGCCAACGC ATTTACGCGC GGCGCAAGCA GACGGTGGAG CGCAGCTTCG CCGATGCCAA GCAGTTGCAT GGGCACCGTT ATGCCCGTAT GCGTGGGCTA CGCAAGGTGG CCGAGCAGTG CTTGCTGGCC GCGGCGGCAC AGAACATCAA GAAGATTGCG ATGCTGCTGG CGCGGAAGCG GAAAAAGGGG CCAGCGGGTC CCGATTGGCG CTTCGTGCGC ATGCTGCTGC GTCTGGTGAG CGGTTTGCGC TGCAGCTTCG ACTACCCGCT CGCGGCGAAC CCGCAATCCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA