SSGCID: Target Page

BupsA.17293.b

ISBma2, transposase

CENTER ID:	BupsA.17293.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17293.b.A1.GE32572	Full length( BupsA.17293.b )		1	487

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17293.b.A1.PS01353	Full length( BupsA.17293.b )		1	487

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.3476
RefSeq:	YP_334708.1
UniProt:	Q3JNZ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKTPMPTQH ELEMVTLEEL VPKDHLLRQI DAAVDFEFIR AKVAHLYCAD NGRPALDPVV MFKLLFIGYL FGVRSERQLM REVQVNVAYR WFARFRLTDK VPDASTFSQN RRRRFTDTTV YQEIFDEIVR QAIKRGLVDG RVLYTDSTHL KANANKGKFD VVKLEQTPAA YTEALNAAVD ADRAAHGKKP LDRDDDEPPS SKDTKISRTD PDSGYMVRDD KPKGFFYLDH RTVDAKHAII TDTHVTPASV HDSQPYLDRL DRQRERFEFK VEAMGLDAGY FTPAVCQGLE ERGIAGVMGY RTPNHKPGMF YKRQFKYDAY RNEYVCPQGQ ALPYSTTNRL GYREYKSNAQ ICGRCPVRSQ CTNSAIAVKV VTRHVWERAK ERVDARRLTE WGQRIYARRK QTVERSFADA KQLHGHRYAR MRGLRKVAEQ CLLAAAAQNI KKIAMLLARK RKKGPAGPDW RFVRMLLRLV SGLRCSFDYP LAANPQS

NT Sequence

ATGCTGAAGA CGCCCATGCC CACGCAGCAC GAACTCGAGA TGGTGACGCT CGAGGAACTC GTGCCGAAGG ACCACCTACT GCGCCAGATC GATGCGGCGG TGGATTTCGA GTTCATCCGC GCGAAGGTGG CGCATCTGTA TTGCGCGGAC AACGGGCGGC CGGCGCTCGA TCCCGTGGTG ATGTTCAAGC TGTTGTTCAT CGGCTACCTG TTCGGGGTGC GCAGCGAGCG GCAACTGATG CGTGAGGTCC AGGTCAACGT CGCCTATCGC TGGTTCGCCC GGTTCCGGCT GACCGACAAG GTGCCGGATG CGTCAACGTT CTCGCAGAAT CGCCGCCGAC GCTTCACGGA CACGACGGTG TATCAGGAGA TCTTCGACGA GATCGTGCGG CAGGCGATCA AGCGCGGGCT GGTCGACGGT CGGGTGCTGT ACACGGACAG CACGCACCTG AAGGCGAACG CGAACAAGGG CAAGTTCGAT GTGGTGAAGC TGGAGCAGAC GCCGGCGGCC TACACGGAGG CATTGAACGC GGCCGTGGAT GCGGACCGGG CCGCGCATGG CAAGAAGCCG CTGGATCGCG ACGACGATGA GCCGCCGTCG AGCAAGGACA CCAAGATCAG CCGGACCGAT CCGGACAGCG GCTACATGGT GCGGGACGAC AAGCCGAAGG GGTTCTTCTA TCTGGACCAC CGCACGGTGG ATGCCAAGCA CGCGATCATC ACCGATACGC ATGTGACGCC GGCCTCGGTG CATGACAGCC AGCCGTATCT GGATCGGCTG GATCGCCAGC GCGAGCGCTT TGAGTTCAAG GTCGAGGCGA TGGGGCTGGA TGCGGGCTAC TTCACGCCGG CGGTGTGCCA GGGGCTGGAG GAGCGAGGGA TTGCCGGGGT GATGGGCTAT CGCACGCCGA ACCACAAGCC GGGCATGTTC TACAAACGGC AGTTCAAGTA CGACGCGTAT CGCAACGAAT ACGTGTGCCC GCAGGGGCAG GCCCTGCCGT ACAGCACGAC CAATCGGCTC GGCTATCGGG AATACAAATC CAATGCGCAG ATCTGCGGGC GCTGCCCGGT ACGATCGCAG TGCACGAACA GTGCGATCGC GGTGAAGGTG GTAACGCGCC ACGTGTGGGA GCGCGCCAAG GAGCGGGTGG ACGCGCGGCG CTTGACCGAA TGGGGCCAAC GCATTTACGC GCGGCGCAAG CAGACGGTGG AGCGCAGCTT CGCCGATGCC AAGCAGTTGC ATGGGCACCG TTATGCCCGT ATGCGTGGGC TACGCAAGGT GGCCGAGCAG TGCTTGCTGG CCGCGGCGGC ACAGAACATC AAGAAGATTG CGATGCTGCT GGCGCGGAAG CGGAAAAAGG GGCCAGCGGG TCCCGATTGG CGCTTCGTGC GCATGCTGCT GCGTCTGGTG AGCGGTTTGC GCTGCAGCTT CGACTACCCG CTCGCGGCGA ACCCGCAATC C

Details for BupsA.17293.b.A1.GE32572

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	58kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Validated AA Sequence

MLKTPMPTQH ELEMVTLEEL VPKDHLLRQI DAAVDFEFIR AKVAHLYCAD NGRPALDPVV MFKLLLIGYL FGVRSERQLM REVQVNVAYR WFARFRLTDK VPDASTFSQN RRRRFTDTTV YQEIFDEIVR QAIKRGLVDG RVLYTDSTHL KANANKGKFD VVKLEQTPAA YTEALNAAVD ADRAAHGKKP LDRDDDEPPS SKDTKISRTD PDSGYMVRDD KPKGFFYLDH RTVDAKHAII TDTHVTPASV HDSQPYLDRL DRQRERFEFK VEAMGLDAGY FTXAVCQGLE ERGIAGVMGY RTPNHKPGMF YKRQFKYDAY RNEYVCPQGQ ALPYSTTNRL GYREYKSNAQ ICGRCPVRSQ CTNSAIAVKV VTRHVWERAK ERVDARRLTE WGQRIYARRK QTVERSFADA KQLHGHRYAR MRGLRKVAEQ CLLAAAAQNI KKIAMLLARK RKKGPAGPDW RFVRMLLRLV SGLRCSFDYP LAAN

Validated NT Sequence

cgggttcgcc gcgagcgggt agtcgaagct gcagcgcaaa ccgctcacca gacgcagcag catgcgcacg aagcgccaat cgggacccgc tggccccttt ttccgcttcc gcgccagcag catcgcaatc ttcttgatgt tctgtgccgc cgcggccagc aagcactgct cggccacctt gcgtagccca cgcatacggg cataacggtg cccatgcagc tgcttggcat cggcgaagct gcgctccacc gtctgcttgc gccgcgcgta aatgcgttgg ccccattcgg tcaagcgccg cgcgtccacc cgctccttgg cgcgctccca cacgtggcgc gttaccacct tcaccgcgat cgcactgttc gtgcactgcg atcgtaccgg gcagcgcccg cagatctgcg cattggattt gtattcccga tagccgagcc gattggtcgt gctgtacggc agggcctgcc cctgcgggca cacgtattcg ttgcgatacg cgtcgtactt gaactgccgt ttgtagaaca tgcccggctt gtggttcggc gtgcgatagc ccatcacccc ggcaatccct cgctcctcca gcccctggca caccgccngc gtgaagtagc ccgcatccag ccccatcgcc tcgaccttga actcaaagcg ctcgcgctgg cgatccagcc gatccagata cggctggctg tcatgcaccg aggccggcgt cacatgcgta tcggtgatga tcgcgtgctt ggcatccacc gtgcggtggt ccagatagaa gaaccccttc ggcttgtcgt cccgcaccat gtagccgctg tccggatcgg tccggctgat cttggtgtcc ttgctcgacg gcggctcatc gtcgtcgcga tccagcggct tcttgccatg cgcggcccgg tccgcatcca cggccgcgtt caatgcctcc gtgtaggccg ccggcgtctg ctccagcttc accacatcga acttgccctt gttcgcgttc gccttcaggt gcgtgctgtc cgtgtacagc acccgaccgt cgaccagccc gcgcttgatc gcctgccgca cgatctcgtc gaagatctcc tgatacaccg tcgtgtccgt gaagcgtcgg cggcgattct gcgagaacgt tgacgcatcc ggcaccttgt cggtcagccg gaaccgggcg aaccagcgat aggcgacgtt gacctggacc tcacgcatca gttgccgctc gctgcgcacc ccgaacaggt agccgatgag caacagcttg aacatcacca cgggatcgag cgccggccgc ccgttgtccg cgcaatacag atgcgccacc ttcgcgcgga tgaactcgaa atccaccgcc gcatcgatct ggcgcagcag gtggtccttc ggcacgagtt cctcgagcgt caccatctcg agttcgtgct gcgtgggcat gggcgtcttc agcatcgaac caggaccctg ggtctgagct tccagggtac cca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLKTPMPTQ HELEMVTLEE LVPKDHLLRQ IDAAVDFEFI RAKVAHLYCA DNGRPALDPV VMFKLLFIGY LFGVRSERQL MREVQVNVAY RWFARFRLTD KVPDASTFSQ NRRRRFTDTT VYQEIFDEIV RQAIKRGLVD GRVLYTDSTH LKANANKGKF DVVKLEQTPA AYTEALNAAV DADRAAHGKK PLDRDDDEPP SSKDTKISRT DPDSGYMVRD DKPKGFFYLD HRTVDAKHAI ITDTHVTPAS VHDSQPYLDR LDRQRERFEF KVEAMGLDAG YFTPAVCQGL EERGIAGVMG YRTPNHKPGM FYKRQFKYDA YRNEYVCPQG QALPYSTTNR LGYREYKSNA QICGRCPVRS QCTNSAIAVK VVTRHVWERA KERVDARRLT EWGQRIYARR KQTVERSFAD AKQLHGHRYA RMRGLRKVAE QCLLAAAAQN IKKIAMLLAR KRKKGPAGPD WRFVRMLLRL VSGLRCSFDY PLAANPQS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaagacg cccatgccca cgcagcacga actcgagatg gtgacgctcg aggaactcgt gccgaaggac cacctactgc gccagatcga tgcggcggtg gatttcgagt tcatccgcgc gaaggtggcg catctgtatt gcgcggacaa cgggcggccg gcgctcgatc ccgtggtgat gttcaagctg ttgttcatcg gctacctgtt cggggtgcgc agcgagcggc aactgatgcg tgaggtccag gtcaacgtcg cctatcgctg gttcgcccgg ttccggctga ccgacaaggt gccggatgcg tcaacgttct cgcagaatcg ccgccgacgc ttcacggaca cgacggtgta tcaggagatc ttcgacgaga tcgtgcggca ggcgatcaag cgcgggctgg tcgacggtcg ggtgctgtac acggacagca cgcacctgaa ggcgaacgcg aacaagggca agttcgatgt ggtgaagctg gagcagacgc cggcggccta cacggaggca ttgaacgcgg ccgtggatgc ggaccgggcc gcgcatggca agaagccgct ggatcgcgac gacgatgagc cgccgtcgag caaggacacc aagatcagcc ggaccgatcc ggacagcggc tacatggtgc gggacgacaa gccgaagggg ttcttctatc tggaccaccg cacggtggat gccaagcacg cgatcatcac cgatacgcat gtgacgccgg cctcggtgca tgacagccag ccgtatctgg atcggctgga tcgccagcgc gagcgctttg agttcaaggt cgaggcgatg gggctggatg cgggctactt cacgccggcg gtgtgccagg ggctggagga gcgagggatt gccggggtga tgggctatcg cacgccgaac cacaagccgg gcatgttcta caaacggcag ttcaagtacg acgcgtatcg caacgaatac gtgtgcccgc aggggcaggc cctgccgtac agcacgacca atcggctcgg ctatcgggaa tacaaatcca atgcgcagat ctgcgggcgc tgcccggtac gatcgcagtg cacgaacagt gcgatcgcgg tgaaggtggt aacgcgccac gtgtgggagc gcgccaagga gcgggtggac gcgcggcgct tgaccgaatg gggccaacgc atttacgcgc ggcgcaagca gacggtggag cgcagcttcg ccgatgccaa gcagttgcat gggcaccgtt atgcccgtat gcgtgggcta cgcaaggtgg ccgagcagtg cttgctggcc gcggcggcac agaacatcaa gaagattgcg atgctgctgg cgcggaagcg gaaaaagggg ccagcgggtc ccgattggcg cttcgtgcgc atgctgctgc gtctggtgag cggtttgcgc tgcagcttcg actacccgct cgcggcgaac ccgcaatcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BupsA.17293.b.A1.PS01353

PURIFICATION DATe:	1/21/2012
CONCENTRATION:	15.84mg/ml
OBSERVED MW:	26kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTGAAGACG CCCATGCCCA CGCAGCACGA ACTCGAGATG GTGACGCTCG AGGAACTCGT GCCGAAGGAC CACCTACTGC GCCAGATCGA TGCGGCGGTG GATTTCGAGT TCATCCGCGC GAAGGTGGCG CATCTGTATT GCGCGGACAA CGGGCGGCCG GCGCTCGATC CCGTGGTGAT GTTCAAGCTG TTGTTCATCG GCTACCTGTT CGGGGTGCGC AGCGAGCGGC AACTGATGCG TGAGGTCCAG GTCAACGTCG CCTATCGCTG GTTCGCCCGG TTCCGGCTGA CCGACAAGGT GCCGGATGCG TCAACGTTCT CGCAGAATCG CCGCCGACGC TTCACGGACA CGACGGTGTA TCAGGAGATC TTCGACGAGA TCGTGCGGCA GGCGATCAAG CGCGGGCTGG TCGACGGTCG GGTGCTGTAC ACGGACAGCA CGCACCTGAA GGCGAACGCG AACAAGGGCA AGTTCGATGT GGTGAAGCTG GAGCAGACGC CGGCGGCCTA CACGGAGGCA TTGAACGCGG CCGTGGATGC GGACCGGGCC GCGCATGGCA AGAAGCCGCT GGATCGCGAC GACGATGAGC CGCCGTCGAG CAAGGACACC AAGATCAGCC GGACCGATCC GGACAGCGGC TACATGGTGC GGGACGACAA GCCGAAGGGG TTCTTCTATC TGGACCACCG CACGGTGGAT GCCAAGCACG CGATCATCAC CGATACGCAT GTGACGCCGG CCTCGGTGCA TGACAGCCAG CCGTATCTGG ATCGGCTGGA TCGCCAGCGC GAGCGCTTTG AGTTCAAGGT CGAGGCGATG GGGCTGGATG CGGGCTACTT CACGCCGGCG GTGTGCCAGG GGCTGGAGGA GCGAGGGATT GCCGGGGTGA TGGGCTATCG CACGCCGAAC CACAAGCCGG GCATGTTCTA CAAACGGCAG TTCAAGTACG ACGCGTATCG CAACGAATAC GTGTGCCCGC AGGGGCAGGC CCTGCCGTAC AGCACGACCA ATCGGCTCGG CTATCGGGAA TACAAATCCA ATGCGCAGAT CTGCGGGCGC TGCCCGGTAC GATCGCAGTG CACGAACAGT GCGATCGCGG TGAAGGTGGT AACGCGCCAC GTGTGGGAGC GCGCCAAGGA GCGGGTGGAC GCGCGGCGCT TGACCGAATG GGGCCAACGC ATTTACGCGC GGCGCAAGCA GACGGTGGAG CGCAGCTTCG CCGATGCCAA GCAGTTGCAT GGGCACCGTT ATGCCCGTAT GCGTGGGCTA CGCAAGGTGG CCGAGCAGTG CTTGCTGGCC GCGGCGGCAC AGAACATCAA GAAGATTGCG ATGCTGCTGG CGCGGAAGCG GAAAAAGGGG CCAGCGGGTC CCGATTGGCG CTTCGTGCGC ATGCTGCTGC GTCTGGTGAG CGGTTTGCGC TGCAGCTTCG ACTACCCGCT CGCGGCGAAC CCGCAATCCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA