BupsA.17292.c

Type IV fimbrial biogenesis protein,PilB

CENTER ID: BupsA.17292.c
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17292.c.A1.GE31049 Full length( BupsA.17292.c ) 1 419
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3669
RefSeq: YP_334897.1
UniProt: Q3JNF6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSATPSNPSF PAVRSAASAL PRPRAPELAD DAPAVGIVAD MLRAAHERDA SDIHVEPGES GWRVRLRIDG VLHEFARPPA HLRDACVTRI KVLARMDIAE RRVPQDGRLR LPLAPGRAGD YRVSSLPTLH GEKLVLRRLE TLPADLSLAA LGFDAAQARG VEAAIGAPHG LILVTGPTGS GKTLSLYCFL QMLNDVSRNV CTVEDPAEIE LDGINQVGVR EKAGLTFAVA LRAFLRQDPD VIMVGEIRDA QTADVALKAA QTGHLVLSTL HTNDAPAAVA RLLDIGVAPY NLAAALRLVT AQRLVRRLCP ACRAPSDAPP ATLRAAGFDA ASIDAGWRPY AANGCAACHG IGYRGRIGIH QVMPLSAELR NLTVARASSA ELARQARAEG MASLRDAALA RVRDGTTSLS EALATTEAA
NT Sequence
ATGTCCGCAA CGCCCTCCAA CCCTTCTTTC CCCGCCGTGC GGTCCGCAGC ATCCGCGCTG CCCCGGCCGC GCGCGCCCGA GCTCGCCGAC GACGCACCCG CGGTCGGTAT CGTCGCCGAC ATGCTGCGCG CGGCCCACGA GCGCGATGCT TCCGACATCC ATGTCGAGCC CGGTGAATCC GGCTGGCGCG TGCGTCTGCG CATCGACGGC GTGCTGCACG AGTTCGCGCG GCCGCCCGCT CATTTGCGCG ACGCATGCGT GACGCGCATC AAGGTACTCG CGCGGATGGA CATCGCCGAG CGCCGCGTGC CGCAGGACGG CCGGCTGCGG CTCCCGCTCG CGCCGGGGCG CGCGGGCGAC TACCGCGTGA GCTCGCTGCC GACGCTGCAT GGCGAGAAAC TCGTGCTGCG GCGTCTGGAG ACGCTGCCCG CCGATCTGTC GCTCGCCGCG CTCGGCTTCG ACGCGGCTCA GGCACGCGGC GTCGAGGCGG CGATCGGCGC GCCGCACGGT CTCATCCTCG TCACGGGGCC GACGGGCAGC GGCAAGACGC TGTCACTGTA TTGCTTCCTG CAAATGCTGA ACGACGTGTC GCGCAACGTC TGCACGGTCG AGGATCCGGC CGAGATCGAG CTCGACGGCA TCAACCAGGT CGGCGTGCGC GAGAAGGCGG GCCTCACCTT CGCGGTCGCG CTGCGCGCGT TCCTGCGGCA GGATCCCGAC GTCATCATGG TCGGCGAGAT ACGCGATGCG CAGACCGCCG ACGTCGCGCT GAAAGCCGCG CAGACCGGGC ACCTCGTGCT GTCGACGCTG CACACGAATG ACGCGCCCGC CGCGGTGGCC CGCCTGCTCG ACATCGGCGT CGCGCCGTAC AACCTCGCCG CCGCGCTGCG GCTCGTGACA GCGCAGCGGC TCGTGCGACG CCTGTGCCCC GCTTGCCGCG CGCCGTCGGA CGCGCCGCCC GCCACACTGC GCGCCGCGGG ATTCGACGCC GCGTCGATCG ACGCCGGCTG GCGCCCGTAT GCCGCGAACG GATGCGCCGC ATGCCATGGA ATCGGTTATC GGGGGCGCAT CGGCATCCAT CAGGTGATGC CGCTGTCCGC CGAGTTGCGC AATCTGACCG TCGCGCGCGC GAGCAGCGCC GAACTCGCGC GACAAGCGCG CGCCGAAGGC ATGGCGAGCC TGCGCGATGC GGCACTCGCC CGCGTTCGCG ACGGCACGAC GAGCTTGAGC GAAGCGCTCG CCACGACGGA GGCGGCA
Details for BupsA.17292.c.A1.GE31049
HARVESTED ON: 12/14/2010
SEQUENCED ON: 12/20/2010
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 66
PERCENT COVERAGE: 12
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSAXXXXXX FPAXXXAXSA LPRXXXPXLX XXAPAXX
Validated NT Sequence
tttgtttaac tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtccgc tgngcnntnn ntnncanntt tccccgccgn gnggnccgca naatccgcnc tgccccgnnc gnnanngccc ganctcgncn acnacgcacc cgctgancnn taactannnn ncntancgnn nnncnncann tnnnnnangc tntnnnnnnc catgtncagn ccggtgaatc cggctggncg tgcgtctgcn catcgacggc gtgctgcacn agttcnngnn nnnncccgct canntnngcg acgcatgcgt gacgcgcatc aaggtactcg ngcggatgga catcgccgan nnncgcgngc cnaacgacgg ccggntgcgg ctcccgtcgc gccggggcgn gcggnngact accgcgtgag ctcgctgccg acgctgcatg nnnagannnn nnngnngcgg cntctggaga cgctgcccgc cgatctgtcg ctcgcnncgc tcggnnttcn acgcggctca ngnacgcnnn nnnnaggcgg cgatnngcgc gccgcanggn cnnntnctcg tcacggggnc gacgggcagc ggnnagacnc tgtcactgta ttnntnccng caaatgctnn nnnacngncn nnnnacgtct gnncgngtcn aggantccgg nngagatcna nntcgacggn atcaancang nnggcgtngn nnnanaaggc nngnnntnnn ntnngcgnnn gnnntgcgnn nnnnnntgcn ggnnnnnntn nnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSATPSNPS FPAVRSAASA LPRPRAPELA DDAPAVGIVA DMLRAAHERD ASDIHVEPGE SGWRVRLRID GVLHEFARPP AHLRDACVTR IKVLARMDIA ERRVPQDGRL RLPLAPGRAG DYRVSSLPTL HGEKLVLRRL ETLPADLSLA ALGFDAAQAR GVEAAIGAPH GLILVTGPTG SGKTLSLYCF LQMLNDVSRN VCTVEDPAEI ELDGINQVGV REKAGLTFAV ALRAFLRQDP DVIMVGEIRD AQTADVALKA AQTGHLVLST LHTNDAPAAV ARLLDIGVAP YNLAAALRLV TAQRLVRRLC PACRAPSDAP PATLRAAGFD AASIDAGWRP YAANGCAACH GIGYRGRIGI HQVMPLSAEL RNLTVARASS AELARQARAE GMASLRDAAL ARVRDGTTSL SEALATTEAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgcaacg ccctccaacc cttctttccc cgccgtgcgg tccgcagcat ccgcgctgcc ccggccgcgc gcgcccgagc tcgccgacga cgcacccgcg gtcggtatcg tcgccgacat gctgcgcgcg gcccacgagc gcgatgcttc cgacatccat gtcgagcccg gtgaatccgg ctggcgcgtg cgtctgcgca tcgacggcgt gctgcacgag ttcgcgcggc cgcccgctca tttgcgcgac gcatgcgtga cgcgcatcaa ggtactcgcg cggatggaca tcgccgagcg ccgcgtgccg caggacggcc ggctgcggct cccgctcgcg ccggggcgcg cgggcgacta ccgcgtgagc tcgctgccga cgctgcatgg cgagaaactc gtgctgcggc gtctggagac gctgcccgcc gatctgtcgc tcgccgcgct cggcttcgac gcggctcagg cacgcggcgt cgaggcggcg atcggcgcgc cgcacggtct catcctcgtc acggggccga cgggcagcgg caagacgctg tcactgtatt gcttcctgca aatgctgaac gacgtgtcgc gcaacgtctg cacggtcgag gatccggccg agatcgagct cgacggcatc aaccaggtcg gcgtgcgcga gaaggcgggc ctcaccttcg cggtcgcgct gcgcgcgttc ctgcggcagg atcccgacgt catcatggtc ggcgagatac gcgatgcgca gaccgccgac gtcgcgctga aagccgcgca gaccgggcac ctcgtgctgt cgacgctgca cacgaatgac gcgcccgccg cggtggcccg cctgctcgac atcggcgtcg cgccgtacaa cctcgccgcc gcgctgcggc tcgtgacagc gcagcggctc gtgcgacgcc tgtgccccgc ttgccgcgcg ccgtcggacg cgccgcccgc cacactgcgc gccgcgggat tcgacgccgc gtcgatcgac gccggctggc gcccgtatgc cgcgaacgga tgcgccgcat gccatggaat cggttatcgg gggcgcatcg gcatccatca ggtgatgccg ctgtccgccg agttgcgcaa tctgaccgtc gcgcgcgcga gcagcgccga actcgcgcga caagcgcgcg ccgaaggcat ggcgagcctg cgcgatgcgg cactcgcccg cgttcgcgac ggcacgacga gcttgagcga agcgctcgcc acgacggagg cggcataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa